Patents by Examiner Dennis M White
  • Patent number: 9568404
    Abstract: Disclosed are devices or apparatus and methods for membrane-, bead- or cell-based biomolecule analysis, where a detectable signal can be measured after affinity binding and/or enzymatic reaction occurs on the membranes, beads or cells. The apparatus or device can rapidly separate the beads or cells from the liquid solution without using centrifugation, vacuum, or magnetic force. The apparatus or device includes a sample well for receiving a sample containing cells or beads, as well as other aqueous solutions, a porous filter membrane at the bottom of the well which is capable of retaining the cells or beads on its upper surface by size exclusion, and an absorbing plug in touch with the filter membrane for removing the liquid solution. Multiple devices can be arranged in a multi-well array in a plate so that signals from multiple assays can be directly analyzed in a plate reader or an imaging instrument.
    Type: Grant
    Filed: March 18, 2015
    Date of Patent: February 14, 2017
    Inventor: Junyu Mai
  • Patent number: 9561506
    Abstract: Fluidic devices and methods including those that provide storage and/or facilitate fluid handling of reagents are provided. Fluidic devices described herein may include channel segments positioned on two sides of an article, optionally connected by an intervening channel passing through the article. The channel segments may be used to store reagents in the device prior to first use by an end user. The stored reagents may include fluid plugs positioned in linear order so that during use, as fluids flow to a reaction site, they are delivered in a predetermined sequence. The specific geometries of the channel segments and the positions of the channel segments within the fluidic devices described herein may allow fluid reagents to be stored for extended periods of time without mixing, even during routine handling of the devices such as during shipping of the devices, and when the devices are subjected to physical shock or vibration.
    Type: Grant
    Filed: September 24, 2013
    Date of Patent: February 7, 2017
    Assignee: OPKO Diagnostics, LLC
    Inventors: Jason Taylor, Vincent Linder
  • Patent number: 9561503
    Abstract: A sensor element comprising a tape-shaped mount film; and a plurality of film-shaped sensors bonded on one surface of the mount film, wherein the front and rear film-shaped sensors are spaced at a predetermined distance in a lengthwise direction of the mount film, and a crosswise length of each film-shaped sensor is shorter by a predetermined quantity than a crosswise length of the mount film.
    Type: Grant
    Filed: May 12, 2015
    Date of Patent: February 7, 2017
    Assignee: ARKRAY, Inc.
    Inventors: Yuji Kurata, Hisashi Kaneda
  • Patent number: 9562253
    Abstract: A method includes obtaining a sample from a patient; applying, to the sample, a reagent to cause bacterial cells to release adenosine triphosphate (ATP) when the sample includes the bacterial cells; applying another reagent that reacts with the ATP to form a colorimetric agent; detecting whether the sample changes in appearance as observed by an unaided eye of a practitioner when a concentration of the agent is greater than a threshold; identifying a time period from when the other reagent is applied to when the change in appearance is detected; and determines a severity, of a bacterial infection, based on the time period. The severity corresponds to a severity level when the time period is less than a duration of a point of care visit by a first amount, or a higher severity level when the time period is less than the duration by more than the first amount.
    Type: Grant
    Filed: June 30, 2014
    Date of Patent: February 7, 2017
    Assignee: POINT OF CARE DIAGNOSTICS, LLC
    Inventor: Ronald Turner
  • Patent number: 9557317
    Abstract: Systems, apparatus, methods, and kits are provided for automated mass spectrometric analysis of small volumes of liquid samples, such as biological samples. The systems, apparatus, and kits may be used in facilities where high throughput of samples, as well as reliable and repeatable assay results with little training of staff, are needed. Such facilities include hospital emergency wards.
    Type: Grant
    Filed: June 25, 2015
    Date of Patent: January 31, 2017
    Assignee: PureHoney Technologies, Inc.
    Inventor: Can Ozbal
  • Patent number: 9551707
    Abstract: A nanopore structure includes an aperture extending from a first surface to a second surface of a substrate, the aperture having a wall comprising gold ions embedded in the substrate, the wall defining a first diameter; a first deoxyribonucleic acid (DNA) layer including a thiolated DNA strand covalently bonded to the embedded gold ions within the wall of the aperture; and a second DNA layer hydrogen bonded to the first DNA layer, the second DNA layer defines a substantially cylindrical nanopore that defines a second diameter within the wall of the aperture, the second DNA layer including a single-stranded DNA strand; wherein the second diameter is less than the first diameter.
    Type: Grant
    Filed: April 30, 2015
    Date of Patent: January 24, 2017
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Yann Astier, Joshua T. Smith
  • Patent number: 9545635
    Abstract: Provided are pipette tip trays for use in biotechnology applications. In certain embodiments, provided are pipette tip trays having one or more of the following features: (i) lacking an external hinge between the lid and the rack, and (ii) having snap plate fasteners configured to releasably secure a snap plate to a base.
    Type: Grant
    Filed: March 17, 2011
    Date of Patent: January 17, 2017
    Assignee: BIOTIX, INC.
    Inventors: Arta Motadel, Peter Paul Blaszcak
  • Patent number: 9543118
    Abstract: A nanopore structure includes an aperture extending from a first surface to a second surface of a substrate, the aperture having a wall comprising gold ions embedded in the substrate, the wall defining a first diameter; a first deoxyribonucleic acid (DNA) layer including a thiolated DNA strand covalently bonded to the embedded gold ions within the wall of the aperture; and a second DNA layer hydrogen bonded to the first DNA layer, the second DNA layer defines a substantially cylindrical nanopore that defines a second diameter within the wall of the aperture, the second DNA layer including a single-stranded DNA strand; wherein the second diameter is less than the first diameter.
    Type: Grant
    Filed: June 19, 2015
    Date of Patent: January 10, 2017
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Yann Astier, Joshua T. Smith
  • Patent number: 9535036
    Abstract: Provided are a multiple discrimination device and a method of manufacturing the same. According to the multiple discrimination device, a three-dimensional micro ferromagnetic pattern is optimally designed and arranged to allow a magnetic force applied to a discrimination-target particle to be discriminated to be well controlled to perform discrimination well. The method employs a semiconductor processing technology, thereby precisely manufacturing and allowing mass production.
    Type: Grant
    Filed: September 17, 2013
    Date of Patent: January 3, 2017
    Assignee: ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE
    Inventors: Dae-Sik Lee, Jeong Won Park, Seong-Mok Cho, Moon Youn Jung
  • Patent number: 9527077
    Abstract: A disposable, point-of-care diagnostic cartridge has a base with a reaction chamber and an inlet and a fluidic channel extending therebetween. An apparatus is fixed to the base. The apparatus includes a flexible upper layer having a reservoir containing fluid therein; a flexible lower layer, and a rigid substrate sandwiched between the upper and lower layers. The upper layer is fixed to the substrate with a first adhesive having a first bond force. The lower layer has a valve member in fluid communication with a through port in the base and the base inlet. The valve member has a closed state to seal off the through port and the inlet from fluid communication and an open state to bring the through port and the inlet into fluid communication. The valve member is moveable from the closed state to the open state under a force less than the first bond force.
    Type: Grant
    Filed: January 29, 2015
    Date of Patent: December 27, 2016
    Inventor: David W. Wright
  • Patent number: 9522397
    Abstract: Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.
    Type: Grant
    Filed: November 30, 2015
    Date of Patent: December 20, 2016
    Assignee: CUE INC.
    Inventors: Ayub Khattak, Clinton Sever
  • Patent number: 9518985
    Abstract: The present invention provides a semi-quantitative lateral flow assay device and method for generating semi-quantitative data from a lateral flow assay. The device comprises a thin, porous hydrophilic substrate wherein the substrate includes a star-shaped or other geometry taken in plan view having a liquid sample-receiving central region and multiple arms that extend or radiate out from the central region. Each arm includes a reaction zone formed by the presence of an analyte-capturing agent wherein the reaction zone of each arm is located at a different distance from the central region such that the analyte in the sample is captured accumulates in different quantities at at least some of the reaction zones of the arms in a manner that can be analyzed to yield semi-quantitative data from lateral flow assays.
    Type: Grant
    Filed: September 11, 2014
    Date of Patent: December 13, 2016
    Assignee: STC.UNM
    Inventor: Scott A. Sibbett
  • Patent number: 9500664
    Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.
    Type: Grant
    Filed: December 30, 2011
    Date of Patent: November 22, 2016
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kevin D. Ness, Christopher F. Kelly, Donald A. Masquelier
  • Patent number: 9480981
    Abstract: An integrated device for a sample collection and transfer is provided. The integrated device comprises a capillary channel disposed between a first layer and a second layer, wherein the first layer comprises a hydrophilic layer comprising a fluid inlet for receiving a sample fluid to the capillary channel, wherein the capillary channel comprises an inner surface and an outer surface and an outlet for driving out the sample fluid. The device further comprises an interface assembly comprising: a third layer, a fourth layer, a fifth layer, and a flow path. The interface assembly is disposed on the outer surface of the capillary, at a determining position relative to the outlet, such that the capillary is in contact with the third layer of the interface assembly and the outlet is in contact with the flow path of the interface assembly for driving out the sample fluid from the integrated device.
    Type: Grant
    Filed: July 25, 2014
    Date of Patent: November 1, 2016
    Assignee: General Electric Company
    Inventors: Ralf Lenigk, Erin Jean Finehout, Xuefeng Wang
  • Patent number: 9476862
    Abstract: A nanostructure sensing device comprises a semiconductor nanostructure having an outer surface, and at least one of metal or metal-oxide nanoparticle clusters functionalizing the outer surface of the nanostructure and forming a photoconductive nanostructure/nanocluster hybrid sensor enabling light-assisted sensing of a target analyte.
    Type: Grant
    Filed: April 12, 2013
    Date of Patent: October 25, 2016
    Assignees: University of Maryland, College Park, The United States of America, as Represented by the Secretary of Commerce, National Institute of Standards and Technology, George Mason University, The George Washington University
    Inventors: Abhishek Motayed, Geetha Aluri, Albert V. Davydov, Mulpuri V. Rao, Vladimir P. Oleshko, Ritu Bajpai, Mona E. Zaghloul
  • Patent number: 9468867
    Abstract: A microfluidic device is provided for inducing the separation of constituent elements from a microfluidic sample by introducing phase changes in the microfluidic sample while contained in a microfluidic channel in the device. At least a portion of the microfluidic sample is frozen to cause fractional exclusion of the constituent element from the frozen portion of the microfluidic sample. Different portions of the microfluidic sample may be frozen in different sectors and at different times in order to cause movement in a desired direction of the separated constituent element. Portions of the microfluidic sample may be frozen in a sequential order of adjacent sectors within the microfluidic channel in order to cause sequential movement of the excluded constituent element toward one portion of the microfluidic channel. The frozen portion of the microfluidic sample is then thawed, wherein the separated constituent element remains substantially separated from the thawed, purified microfluidic sample.
    Type: Grant
    Filed: December 24, 2013
    Date of Patent: October 18, 2016
    Assignee: The Aerospace Corporation
    Inventor: Richard P. Welle
  • Patent number: 9469529
    Abstract: The present invention provides a fiber base sensor and the fabricating method thereof. More specifically, one of the main goals of the present invention is to solve the problem existing in the prior art of the failure of forming a plurality of nano-particles on the surface of the fiber base sensor evenly by utilizing self-assembly process with the nano-imprint process. Furthermore, the another goal of the present invention is to provide detail parameters required in the said nano-imprinting process for avoiding the efficiency drop caused by over-embedding the nano-particles into the fiber based sensor.
    Type: Grant
    Filed: March 23, 2015
    Date of Patent: October 18, 2016
    Assignee: National Tsing Hua University
    Inventors: De-Hui Wan, Shih-Yu Tseng, Chung-Yao Yang, Szu-Ting Lin, Chao-Min Cheng
  • Patent number: 9453812
    Abstract: An electrochemical-based analytical test strip for the determination of an analyte (such as glucose) in a bodily fluid sample (for example, a whole blood sample) and/or a characteristic of the bodily fluid sample (e.g., hematocrit) includes a sample-entry chamber with a sample-application opening disposed on an end edge of the electrochemical-based analytical test strip, and first and second sample-determination chambers, each in direct fluidic communication with the sample-entry chamber. The electrochemical-based analytical test strip also includes first and second electrodes (such as first and second hematocrit electrodes) disposed in the first sample-determination chamber, and a third and fourth electrodes (for example working and reference electrodes) disposed in the second sample-determination chamber.
    Type: Grant
    Filed: June 24, 2014
    Date of Patent: September 27, 2016
    Assignee: LifeScan Scotland Limited
    Inventors: Alexander Cooper, Antony Smith, Lynsey Whyte, Neil Whitehead, David McColl, Brian Guthrie, Timothy Lloyd, Rossano Massari, Christian Forlani
  • Patent number: 9452431
    Abstract: A chemical assay device includes a hydrophilic substrate and one or more hydrophobic structures that extend from a first side of the hydrophilic substrate to a second side of the hydrophilic substrate. A hydrophobic structure in the hydrophilic substrate forms a fluid barrier wall that extends from the first side of the hydrophilic substrate to the second side of the hydrophilic substrate with a deviation of less than 20° from a perpendicular axis between the first side and the second side. The hydrophobic structure is formed from a wax or a phase change ink.
    Type: Grant
    Filed: April 14, 2016
    Date of Patent: September 27, 2016
    Assignee: Xerox Corporation
    Inventors: Jing Zhou, Mandakini Kanungo, Nancy Y. Jia, Wei Hong
  • Patent number: 9448220
    Abstract: A system and method for analyzing contaminants such as hydrocarbons in soil and ground water utilizes a reaction device comprising a catalyst encapsulated in a permeable material and processes that device in contact with a contaminant in an analytical device in order to generate a spectrogram indicative of the contaminants in the soil and ground water.
    Type: Grant
    Filed: January 18, 2013
    Date of Patent: September 20, 2016
    Inventor: John David Hanby