Patents by Inventor John D. Gearhart

John D. Gearhart has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for obtaining human embryoid body-derived cells
    Patent number: 7795026
    Abstract: The invention is directed to novel cells that are derived from human embryoid bodies. Such embryoid body-derived (EBD) cells are relatively uncommitted or progenitor (e.g., pluripotent) cells. EBD cells, while not immortal, display long-term proliferation in culture with a normal karyotype and can be cryopreserved and cloned. They can be efficiently transfected with retroviruses and lentivirus and genetically manipulated. Although they have a developmentally broad multilineage expression profile, they do not form tumors when injected into severe combined immunodeficiency (SCID) mice. As a result, EBD cells have a variety of uses, for example, in transplantation therapies.
    Type: Grant
    Filed: January 22, 2001
    Date of Patent: September 14, 2010
    Assignee: The Johns Hopkins University School of Medicine
    Inventors: Michael J. Shamblott, John D. Gearhart
  • Human embryoid body-derived cells
    Publication number: 20030175954
    Abstract: The invention is directed to novel cells that are derived from human embryoid bodies. Such embryoid body-derived (EBD) cells are relatively uncommitted or progenitor (e.g., pluripotent) cells. EBD cells, while not immortal, display long-term proliferation in culture with a normal karyotype and can be cryopreserved and cloned. They can be efficiently transfected with retroviruses and lentivirus and genetically manipulated. Although they have a developmentally broad multilineage expression profile, they do not form tumors when injected into severe combined immunodeficiency (SCID) mice. As a result, EBD cells have a variety of uses, for example, in transplantation therapies.
    Type: Application
    Filed: January 22, 2001
    Publication date: September 18, 2003
    Inventors: Michael J. Shamblott, John D. Gearhart
  • Human embryonic germ cell line and methods of use
    Publication number: 20030148514
    Abstract: Primordial germ cells isolated from human embryonic tissue, such as from the gonadal ridges of human embryo, are disclosed. The primordial germ cells are cultured resulting in cells that resemble embryonic stem cells or embryonic germ cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.
    Type: Application
    Filed: February 7, 2003
    Publication date: August 7, 2003
    Applicant: THE JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE
    Inventors: John D. Gearhart, Michael Joseph Shamblott
  • Differentiation of human embryonic germ cells
    Patent number: 6562619
    Abstract: Primordial germ cells isolated from human embryonic tissue, such as from the gonadal ridges of human embryo, are disclosed. The primordial germ cells are cultured resulting in cells that resemble embryonic stem cells or embryonic germ cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.
    Type: Grant
    Filed: April 20, 2000
    Date of Patent: May 13, 2003
    Assignee: The Johns Hopkins University School of Medicine
    Inventors: John D. Gearhart, Michael Joseph Shamblott
  • Human enbryonic germ cell and methods of use
    Patent number: 6331406
    Abstract: Primordial germ cells extracted from post blastocyst human embryos, such as from the gonadal ridges of a 8-11 week LMP human embryo, are disclosed. The primordial germ cells are cultured in long term culture (more than 30 days) resulting in cells that resemble embryonic stem cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.
    Type: Grant
    Filed: December 12, 1997
    Date of Patent: December 18, 2001
    Assignee: The John Hopkins University School of Medicine
    Inventors: John D. Gearhart, Michael Joseph Shamblott
  • Human embryonic germ cell line and methods of use
    Patent number: 6245566
    Abstract: Primordial germ cells isolated from human embryonic tissue, such as from the gonadal ridges of human embryo, are disclosed. The primordial germ cells are cultured resulting in cells that resemble embryonic stem cells or embryonic germ cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.
    Type: Grant
    Filed: March 31, 1998
    Date of Patent: June 12, 2001
    Assignee: The Johns Hopkins University School of Medicine
    Inventors: John D. Gearhart, Michael Joseph Shamblott
  • Human embryonic pluripotent germ cells
    Patent number: 6090622
    Abstract: Primordial germ cells extracted from post blastocyst human embryos, such as from the gonadal ridges of a 8-11 week LMP human embryo, are disclosed. The primordial germ cells are cultured in long term culture (more than 30 days) resulting in cells that resemble embryonic stem cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.
    Type: Grant
    Filed: March 31, 1997
    Date of Patent: July 18, 2000
    Assignee: The Johns Hopkins School of Medicine
    Inventors: John D. Gearhart, Michael Joseph Shamblott