Patents by Inventor Michael N. Mindrinos
Michael N. Mindrinos has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230348982Abstract: The invention relates to polynucleotide probes, with each polynucleotide probe comprising two perfectly complementary strands. In some embodiments, each one of the strands comprises, in a 5? to 3? direction, a) a first target hybridization sequence, b) a first digital tag sequence, c) a first Halo barcode sequence, d) a first Halo amplification primer sequence, e) a reverse second Halo amplification primer sequence, f) a reverse second Halo barcode sequence, g) a reverse second digital tag sequence, and h) a reverse second target hybridization sequence. The invention also relates to methods of using these novel probes in to determine the levels of a minor population of DNA amongst a mixture of DNA from two different sources.Type: ApplicationFiled: June 4, 2021Publication date: November 2, 2023Inventors: Sujatha KRISHNAKUMAR, Ming LI, Peggy PALSGAARD, Michael N. MINDRINOS
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Publication number: 20190002979Abstract: Methods are provided to determine the entire genomic region of a particular HLA locus including both intron and exons. The resultant consensus sequences provides linkage information between different exons, and produces the unique sequence from each of the two genes from the individual sample being typed. The sequence information in intron regions along with the exon sequences provides an accurate HLA haplotype.Type: ApplicationFiled: March 20, 2018Publication date: January 3, 2019Inventors: Chunlin Wang, Michael N. Mindrinos, Mark M. Davis, Ronald W. Davis, Sujatha Krishnakumar
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Patent number: 9981273Abstract: The procedure of dielectric electrophoresis (dielectrophoresis or DEP) utilizes field-polarized particles that move under the application of positive (attractive) and/or negative (repulsive) applied forces. This invention uses negative dielectric electrophoresis (negative dielectrophoresis or nDEP) within a microchannel separation apparatus to make particles move (detached) or remain stationary (attached). In an embodiment of the present invention, the nDEP force generated was strong enough to detach Ag-Ab (antigen-antibody) bonds, which are in the order of 400 pN (piconewtons) while maintaining the integrity of the system components.Type: GrantFiled: September 30, 2013Date of Patent: May 29, 2018Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Mehdi Javanmard, Sam Emaminejad, Janine Mok, Michael N. Mindrinos
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Patent number: 9920370Abstract: Methods are provided to determine the entire genomic region of a particular HLA locus including both intron and exons. The resultant consensus sequences provides linkage information between different exons, and produces the unique sequence from each of the two genes from the individual sample being typed. The sequence information in intron regions along with the exon sequences provides an accurate HLA haplotype.Type: GrantFiled: April 24, 2015Date of Patent: March 20, 2018Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Chunlin Wang, Michael N. Mindrinos, Mark M. Davis, Ronald W. Davis, Sujatha Krishnakumar
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Patent number: 9562269Abstract: Methods are provided to determine the entire genomic region of a particular HLA locus including both intron and exons. The resultant consensus sequences provides linkage information between different exons, and produces the unique sequence from each of the two genes from the individual sample being typed. The sequence information in intron regions along with the exon sequences provides an accurate HLA haplotype.Type: GrantFiled: January 22, 2014Date of Patent: February 7, 2017Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Chunlin Wang, Michael N. Mindrinos, Mark M. Davis, Ronald W. Davis, Sujatha Krishnakumar
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Patent number: 9267943Abstract: Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.Type: GrantFiled: October 28, 2011Date of Patent: February 23, 2016Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Ronald W. Davis, Stefanie S. Jeffrey, Michael N. Mindrinos, R. Fabian Pease, Ashley Ann Powell, AmirAli Hajhossein Talasaz
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Publication number: 20150379195Abstract: Methods are provided to determine the genomic sequence of the alleles at the HLA gene. The resultant sequences provide linkage information between different exons, and produces the unique sequence at each gene from the two alleles of the individual sample being typed. The sequence information provides an accurate HLA haplotype. Methods to decrease allele dropout during long range PCR reactions are also disclosed.Type: ApplicationFiled: June 24, 2015Publication date: December 31, 2015Inventors: Chunlin Wang, Michael N. Mindrinos, Mark M. Davis, Ronald W. Davis, Sujatha Krishnakumar, Konstantinos Barsakis, Marcelo Anibal Fernandez-Vina
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Publication number: 20150225789Abstract: Methods are provided to determine the entire genomic region of a particular HLA locus including both intron and exons. The resultant consensus sequences provides linkage information between different exons, and produces the unique sequence from each of the two genes from the individual sample being typed. The sequence information in intron regions along with the exon sequences provides an accurate HLA haplotype.Type: ApplicationFiled: April 24, 2015Publication date: August 13, 2015Inventors: Chunlin Wang, Michael N. Mindrinos, Mark M. Davis, Ronald W. Davis, Sujatha Krishnakumar
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Publication number: 20140206547Abstract: Methods are provided to determine the entire genomic region of a particular HLA locus including both intron and exons. The resultant consensus sequences provides linkage information between different exons, and produces the unique sequence from each of the two genes from the individual sample being typed. The sequence information in intron regions along with the exon sequences provides an accurate HLA haplotype.Type: ApplicationFiled: January 22, 2014Publication date: July 24, 2014Inventors: Chunlin Wang, Michael N. Mindrinos, Mark M. Davis, Ronald W. Davis, Sujatha Krishnakumar
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Publication number: 20140102901Abstract: The procedure of dielectric electrophoresis (dielectrophoresis or DEP) utilizes field-polarized particles that move under the application of positive (attractive) and/or negative (repulsive) applied forces. This invention uses negative dielectric electrophoresis (negative dielectrophoresis or nDEP) within a microchannel separation apparatus to make particles move (detached) or remain stationary (attached). In an embodiment of the present invention, the nDEP force generated was strong enough to detach Ag-Ab (antigen-antibody) bonds, which are in the order of 400 pN (piconewtons) while maintaining the integrity of the system components.Type: ApplicationFiled: September 30, 2013Publication date: April 17, 2014Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITYInventors: Mehdi Javanmard, Sam Emaminejad, Janine Mok, Michael N. Mindrinos
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Patent number: 8614056Abstract: Embodiments of the invention are related to microfluidic devices for detecting or determining the concentration of biomolecules in an analyte comprising: a channel, wherein a surface of said channel is fabricated to be functionalized with at least one molecule selected to interact with a biomolecule, said channel being configured to interact with a microsphere, wherein a surface of said microsphere is fabricated to be functionalized with at least one same or different molecule selected to interact with said biomolecule; a second channel in fluid communication with said first channel; a system to move fluid containing said microsphere through said first and second channels; and a system to measure a change in electrical impedance or optical microscopy across said second channel as said microsphere moves through said second channel. Other embodiments concern related devices, and methods of making and using.Type: GrantFiled: March 23, 2011Date of Patent: December 24, 2013Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Ronald W. Davis, Mehdi Javanmard, Michael N. Mindrinos, Janine A. Mok
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Publication number: 20120045828Abstract: Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.Type: ApplicationFiled: October 28, 2011Publication date: February 23, 2012Applicant: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Ronald W. Davis, Stefanie S. Jeffrey, Michael N. Mindrinos, R. Fabian Pease, Ashley Ann Powell, AmirAli Hajhossein Talasaz
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Publication number: 20110312518Abstract: Embodiments of the invention are related to microfluidic devices for detecting or determining the concentration of biomolecules in an analyte comprising: a channel, wherein a surface of said channel is fabricated to be functionalized with at least one molecule selected to interact with a biomolecule, said channel being configured to interact with a microsphere, wherein a surface of said microsphere is fabricated to be functionalized with at least one same or different molecule selected to interact with said biomolecule; a second channel in fluid communication with said first channel; a system to move fluid containing said microsphere through said first and second channels; and a system to measure a change in electrical impedance or optical microscopy across said second channel as said microsphere moves through said second channel. Other embodiments concern related devices, and methods of making and using.Type: ApplicationFiled: March 23, 2011Publication date: December 22, 2011Applicant: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Ronald W. Davis, Mehdi Javanmard, Michael N. Mindrinos, Janine A. Mok
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Patent number: 8071395Abstract: Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.Type: GrantFiled: December 11, 2008Date of Patent: December 6, 2011Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Ronald W. Davis, Stefanie S. Jeffrey, Michael N. Mindrinos, R. Fabian Pease, Ashley Ann Powell, AmirAli Hajhossein Talasaz
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Publication number: 20090220979Abstract: Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.Type: ApplicationFiled: December 11, 2008Publication date: September 3, 2009Applicant: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Ronald W. Davis, Stefanie S. Jeffrey, Michael N. Mindrinos, R. Fabian Pease, Ashley Ann Powell, AmirAli Hajhossein Talasaz