Abstract: The invention provides transgenic nonhuman mammals producing phosphorylated lysosomal proteins in their milk, and methods of generating the same. Phosphorylation occurs at the 6' position of a mannose side chain residue. Also provided are methods of purifying lysosomal proteins from milk, and incorporating the proteins into pharmaceutical compositions for use in enzyme replacement therapy.

Abstract: Production of human procollagen or collagen in cells which ordinarily do not produce these molecules is effected by constructing expression systems compatible with mammary glands of non-human mammals. For example, expression systems can be microinjected into fertilized oocytes and reimplanted in foster mothers and carried to term in order to obtain transgenic non-human mammals capable of producing milk containing recombinant human procollagen or collagen. Human procollagen or collagen produced in this manner can be made of a single collagen type uncontaminated by other human or non-human collagens.

Abstract: A device for detecting, in the early stages of infection, the presence of mastitis, as well as other impurities, in an individual cow or other milk producing animal, using a filter, preferably made of polyester and being disposable, which filters all milk being extracted from the cow, the filter being held in a container placed in the individual vacuum line of a milking machine being used to extract milk from the cow, the claws of the milking machine placed on the cow dropping from the cow upon the presence of mastitis, without contamination of the milk held in the bulk storage facility, while permitting healthy milk to pass through the filter without interruption of the milking process.

Abstract: A method for early detection of mastitis in cows, effected during normal milking of cows in a milking parlor, by monitoring the milk taken from each cow for the presence of milk clots, comprises cutting the vacuum milk line to each milking station and inserting a filter-detector therein between the claw and the recorder jar. Each filter-detector comprises a flat-sided transparent casing with a lateral slot which receives a self-sealing slide carrying the filter element. The slide carrier preferably has an integral bypass aperture so that vacuum conditions at the claw end are retained even after the filter element has become blocked. The slide carrier is preferably constructed so that, when in position in the casing, a seepage passage remains between the slide and the casing inner wall, so that cleansing fluid can pass during normal in-place cleaning of the installation.