Abstract: A crosslinked fluoropolymer resin is configured to include a measuring step of irradiating a surface of the crosslinked fluoropolymer resin with a laser to measure a Raman spectrum, and an acceptance or rejection decision step of determining an acceptance or a rejection of a quality of a measurement region irradiated with the laser, on the basis of an intensity of a fluorescence spectrum relative to an intensity of a Raman scattering peak, which is ascribed to a CF2 stretching vibration, in the measured Raman spectrum.

Abstract: A laser device comprises a first optical cavity comprising a first gain medium and a second optical cavity comprising a second gain medium. The first gain medium and the second gain medium generate light at respective different ranges of wavelengths. A synchronizer is optically coupled to both the first optical cavity and the second optical cavity and is configured to synchronize and mode-lock light from the first optical cavity and the second optical cavity. The laser device also includes a first optical filter and a second optical filter to filter the light from the first optical cavity and the second optical cavity respectively to output first filtered light pulses at a first predetermined range of wavelengths and second filtered light pulses at a second predetermined range of wavelengths.

Abstract: An information processing device acquires an image captured by a transmission electron microscope. The information processing device, for each partial region in the image, executes a two-dimensional Fourier transform on an image of the partial region. The information processing device, based on results obtained by executing the two-dimensional Fourier transform on each of the partial regions, performs clustering of frequency strengths obtained from the results of the two-dimensional Fourier transform. The information processing device determines regions of different crystallinity in the image, based on results of the clustering.

Abstract: An optical measurement probe for capturing a spectral response through an intervening material emitting unwanted background radiation includes: a first lens configured to receive light and collimate the light into a collimated excitation beam defining a first aperture; an objective element for focusing the collimated excitation beam to a point or region in a sample through the intervening material, wherein the objective element also receives light scattered by the sample and the intervening material and collimates the scattered light into a collimated collection beam defining a second aperture; and a blocking element within the collimated collection beam for removing the light scattered by the intervening material from the collimated collection beam received from the sample, wherein the second aperture defined by the collimated collection beam is at least two times greater than the first aperture defined by the collimated excitation beam.

Abstract: Embodiments herein describe spectroscopy systems that use an unmodulated reference optical signal to mitigate noise, or for other advantages. In one embodiment, the unmodulated reference optical signal is transmitted through the same vapor cell as a modulated pump optical signal. As such, the unmodulated reference optical signal experiences absorption by the vapor, which converts laser phase noise to amplitude noise like the other optical signals passing through the vapor cell. In one embodiment, the unmodulated reference optical signal has an optical path in the gas cell that is offset (or non-crossing) from the optical path of the modulated pump optical signal. The unmodulated reference optical signal allows removal or mitigation of the noise on the other optical signal.

Abstract: Method for characterising a microorganism, comprising: —illuminating, in the wavelength range of 390 nm-900 nm, the microorganism having a natural electromagnetic response in said range; —acquiring, in said range, a light intensity reflected by said (or transmitted through said) illuminated microorganism; and —determining the microorganism as being a yeast or a bacterial strain according to the light intensity acquired in said range.

Abstract: Raman systems and methods use advantages offered by increased laser mobility/path length and photon migration to analyze diffusively scattering solids, including pharmaceuticals. A collimated laser excitation beam having a first diameter induces from a sample a backscattered collimated Raman collection beam with a second diameter. The collimated laser excitation beam and the collimated Raman collection beam form a counter-propagating collimated optical path, and the collimated laser excitation beam is preferably smaller in diameter than the diameter of the backscattered collimated Raman beam. The collection beam to a spectrograph for Raman analysis of the sample. A Raman calibration standard may be placed in the collimated optical path, and/or the sample may be supported in a reflective holder that may be at least partially spherical and/or may form part of a multi-well plate. The counter-propagating collimated optical path may be contained within a Raman microscope.

Abstract: An assembly including a non-linear element configured for generating broadband radiation from input radiation coupled into the non-linear element. The assembly further includes an optical element positioned downstream of the non-linear element configured to reflect a fraction of the broadband radiation back into the non-linear element. The non-linear element can be a nonlinear fiber, such as a hollow-core photonic crystal fiber (HC-PCF).

Abstract: A spectroscopic detector comprises a first housing, a second housing, a camera lens housed in the first housing, and an image sensor housed in the second housing. The first housing has a first through hole formed therein. The second housing has a second through hole formed therein. The second housing is attached to the first housing so as to allow for communication between an inside of the second housing and an inside of the first housing via the first through hole and the second through hole. In a state where the second housing is attached to the first housing, a periphery of the second through hole is located inside a periphery of the first through hole.

Abstract: Quantum entanglement protection is disclosed. An entanglement checker receives, from a requestor, a request associated with a first qubit. In response to receiving the request, the entanglement checker accesses qubit entanglement information that identifies an entanglement status of the first qubit. The entanglement checker determines, based on the qubit entanglement information, the entanglement status of the first qubit, and sends a response to the requestor based on the entanglement status.

Abstract: A Raman spectroscopy apparatus using a nano printing device is provided to perform Raman spectroscopy on nanoscale nanostructures printed from the nano printing device. The Raman spectroscopy apparatus includes a laser light source configured to generate and emit a laser light to the nanostructures, and a Raman detector configured to collect spectroscopic information from the light scattered by the nanostructures.

Abstract: A sample measurement device includes: a sample container that stores a sample solution; a light source that irradiates the sample container with irradiation light from a first direction; an imaging part that captures an image of the sample solution based on light scattered by the sample solution from a second direction intersecting the first direction; and a calculator that calculates an absorbance or a concentration of the sample solution based on the image. The calculator calculates a degree of attenuation of a light amount of the image at a constant optical path length along the first direction based on the image, and calculates the absorbance or concentration of the sample solution according to the degree of attenuation.

Abstract: The multi-photon microscope comprises a repetition rate tuner that lowers an optical pulse train emitted from a pulsed laser to a repetition rate for time-gated detection, a scanner that scans the optical pulse train transmitted from the repetition rate tuner in x-axis and y-axis directions, an objective lens that irradiates an optical signal scanned by the scanner to the sample and acquires a fluorescence signal emitted from the excited fluorescent material, a photodetector that photoelectrically converts the fluorescence signal acquired by the objective lens, an amplifier that converts a current signal output from the photodetector into a voltage signal, a digitizer that samples the voltage signal output from the amplifier, and a computing device that separates sampling data output from the digitizer with a detection window set in time domain, and generates an image based on the sampling data separated by the detection window.

Abstract: To provide an article inspection apparatus capable of sensitively and stably detecting a change in a spectrum when an unspecified foreign substance is contained and determining a defective product. A transport unit that transports a tablet for inspection to an article inspection position, a light irradiation unit that irradiates the tablet transported to the article inspection position with light, a light detection unit that detects light transmitted through the tablet, and an article inspection unit that inspects a quality of the tablet based on spectral characteristics of the light detected by the light detection unit are provided, and the article inspection unit standardizes a measured value of a spectrum of the light detected by the light detection unit for each wavelength and determines whether the tablet is a normal product or a defective product based on a standardized value.

Abstract: An apparatus for disruption of tissue. The apparatus includes a housing; a source of pulsed laser radiation; and an optical waveguide. The optical waveguide is configured to transmit the pulsed laser radiation from the source of pulsed laser radiation, and is coupleable to the source of pulsed laser radiation at a proximal end of the optical waveguide to receive the pulsed laser radiation from the source of pulsed laser radiation. The apparatus also includes a driving mechanism coupled to the optical waveguide for controllably changing the position of the optical waveguide relative to a distal end of the housing.

Abstract: A device for performing Raman spectroscopy measurements that incorporate Raman Shift calibration and related method for carrying out the Raman Shift calibration are disclosed. The device comprises of one or more Raman shift reference materials with one or more Raman bands, the positions of which are pre-determined to a high degree of accuracy within a useful temperature range. The device further comprises a sensor to measure the temperature of the one or more reference materials to provide accurate reference values for the Raman shift calibration. When used with the measured spectrum of the reference materials, an accurate Raman Shift calibration function of the device can be generated.

Abstract: An optical calibration tool includes a first body, a light emitter, a light receiver, a second body, and a light reflecting member. The first body has a first engaging port and a second engaging port. The light emitter and the light receiver are disposed in the first body. The second body has a third engaging port and a channel communicated with each other. The third engaging port is configured to selectively engage one of the first engaging port and the second engaging port. When the third engaging port is engaged with the first engaging port, the light emitter is optically coupled to the light reflecting member. When the third engaging port is engaged with the second engaging port, the light receiver is optically coupled to the light reflecting member.

Abstract: Disclosed are a system and method for active stabilization of parasitic fringes in optical spectrometers, wherein the spectrometer obtains the transmission spectrum, and a signal processor extracts the etalon drift from the spectral signatures of parasitic fringes. The disclosed approach improves spectrometer accuracy, minimizes drift, and increases time between calibrations.

Abstract: An embodiment of a module system configured to interface with a microscope is described that comprises an input optical fiber configured to provide an excitation light beam from an external light source; dynamic alignment mirrors configured to adjust the position of the beams paths of the excitation light beam on a first plane; a coupling comprising a first end configured to engage with a complementary end, wherein the excitation light reflects off a turning mirror and travels along a beam path on a second plane through an orifice in the coupling; and an output optical fiber for delivering light from a sample to an external detector, wherein the light from the sample travels along the beam path on the second plane through the orifice in the coupling, reflects off the turning mirror and travels along one of the beam paths on the first plane to the output optical fiber.

Abstract: A device for the detection of analytes comprises a substrate (10) with nano-antennas (11,12). The nano-antennas (11,12) comprise an antenna material (11m, 12m) for forming resonant antenna structures which receive and resonantly interact with source light (L0) to form respective resonance peaks (R1,R2) over a resonant wavelength range (A1,A2) overlapping respective signature wavelength (?1,?2) of a target analyte (A). The resonant interaction causes a locally concentrated field (Ec) of the source light (L0) in the resonant wavelength range (?1,?2). The concentrated intensity (Ic) is localized around a respective target location (T1,T2) which is provided with a sorption material (11s, 12s) that sorbs the target analyte (A). This provides a locally increased analyte concentration (Ac) of the target analyte (A) coinciding with the locally concentrated field (Ec) of the source light (L0). Accordingly, the interaction of the source light (L0) with the target analyte (A) is enhanced.

Abstract: A laser pulse shaping device and method, a pulse shaper, and an optical system are provided. The laser pulse shaping device includes a pulse shaper provided in an optical path connected to a laser source, a laser detection device provided at an actual position of a target of a laser pulse, and the laser source configured to generate the laser pulse, which is transmitted to the target through the optical path. The laser detection device is configured to measure and send an optical parameter of the laser pulse transmitted to the target to the control device. The control device is configured to calculate a phase compensation parameter according to the optical parameter and control the pulse shaper to compensate a phase of the laser pulse transmitted through the optical path according to the phase compensation parameter, such that the optical parameter of the laser pulse reaches a target value.

Abstract: An integrated device for the detection of cancerous tissue including an optical fiber configured to receive at a first end modulated infrared light and to conduct the modulated infrared light from the first end to a second end; and a plasmonic metasurface, disposed on the second end of the optical fiber, configured to localize evanescent infrared light to sub-I 00 nanometer distances from the plasmonic metasurface of the optical fiber such that the localized evanescent infrared light penetrates only the membrane portion of a cell held against the second end, wherein the second end is configured to receive reflected light reflected from the membrane portion the cell, the reflected light including spectroscopic information indicative of whether the cell is noncancerous or cancerous.

Abstract: A spectroscopic measurement apparatus includes a pulsed laser light source that emits pulsed laser light, a beam splitter that splits the pulsed laser light into pump light and probe light, a delay circuit that changes a delay time of the pump light with respect to the probe light, a chopper that intensity-modulates the pump light, a wavelength converter that wavelength-converts the probe light into vacuum ultraviolet light, an optical system that guides the pump light and the wavelength-converted probe light to a sample, and a detector that detects the probe light reflected by the sample.

Abstract: A method and a system for injecting multiple tracer tag fluids into the wellbore are described. The method includes determining multiple injection concentrations of multiple respective tracer tag fluids, determining an injection sequence of the tracer tag fluids into a wellbore, and injecting the tracer tag fluids into the wellbore according to the injection concentrations and the injection sequence. The tracer tag fluids include synthesized polymeric nanoparticles suspended in a solution. The synthesized polymeric nanoparticles are configured bind to a wellbore cutting. The synthesized polymeric nanoparticles are configured to undergo a thermal de-polymerization at a respective temperature and generate a unique mass spectra. The injection sequence includes an injection duration determined by a depth interval of the wellbore to be tagged by the synthesized polymeric nanoparticles and an injection pause to prevent mixing the multiple tracer tag fluids in the wellbore.

Abstract: The present invention relates to a surface-enhanced Raman scattering substrate and a method of fabricating the same. More particularly, the surface-enhanced Raman scattering substrate according to an embodiment includes a substrate; a lower plasmonic layer formed on the substrate and based on a first metal nanostructure; an oxide layer formed on the lower plasmonic layer; and an upper plasmonic layer formed on the oxide layer and based on a second metal nanostructure.

Abstract: Disclosed is a spectroscopic apparatus includes a laser irradiation device that receives an orthogonal code including a series of bits each having a first value or a second value, generates a control signal having a pulse that has a width shorter than a width of a bit section in the bit section corresponding to a bit having the first value, generates a pulsed laser beam having a pulse width shorter than the bit section using the pulse as a trigger, and irradiates an incident beam including the generated pulsed laser beam to a sample, and a detector that receives a detection signal generated from the sample and the orthogonal code, generates an orthogonal code signal of the same waveform as that of the incident beam, based on the orthogonal code, and demodulates a Raman signal, based on a correlation between the generated orthogonal code signal and the Raman signal.

Abstract: A handheld Surface-Enhanced Raman Spectroscopy (SERS) device for detecting phenylalanine (Phe) in a sample collected from a subject, the device comprising a laser generator configured to produce a laser beam; a nanoporous anodic aluminum oxide (NAAO) substrate configured to receive the sample collected from the subject; and a light sensor configured to receive a light.

Abstract: Components resolved in time by a thermal desorption separator accumulate in a sample cell and are analyzed by electromagnetic radiation-based spectroscopic techniques.

Abstract: Ultra-miniature spatial heterodyne spectrometers (SHSs) are presented. Ultra-miniature SHSs in accordance with the invention, comprise a beam-splitter and gratings configured to generate a fringe pattern for spectroscopic detection. Many embodiments include input optics and a sensor and are configured in a way to omit collimating optics and imaging optics from the SHS. Compared to conventional SHSs known in the art, the present invention enables fewer parts, significantly smaller and lighter SHSs, are more efficient and robust, and require less maintenance. Many embodiments are field-deployable, in that such embodiments can be deployed for hand held use in real-world or remote activities outside of research or diagnostic facilities.

Abstract: A calibration curve setting method used at the time of quantitatively analyzing specific components in a drug by a transmission Raman spectrum, the method comprising the steps of: obtaining respective transmission Raman spectra of a plurality of different wave number ranges including Raman hands corresponding to the specific components of a plurality of known drugs of which concentrations or amounts of the specific components are known and the concentrations or the amounts are different from each other; calculating candidate calibration curves which are candidates for calibration curves used for the quantitative analysis respectively from a plurality of transmission. Raman spectra in each of the wave number ranges; and using the most probable candidate calibration curve as a calibration curve for the quantitative analysis of the specific components, among the respective candidate calibration curves.

Abstract: The present invention provides a transmission guided-mode resonant grating integrated spectroscopy device (transmission GMRG integrated spectroscopy device) characterized by comprising, disposed in this order on an optical detector array in which a plurality of diodes are mounted on a substrate made of a semiconductor: a transparent spacer layer; a waveguide layer; a transparent buffer layer provided as desired; a transmission metallic grating layer having a thickness causing surface plasmon; and a transparent protection film layer which is provided as desired.

Abstract: An optical member utilizing light from a point light source is enabled to visually perceive a reproduced optical image with a desired color. An optical modulation device includes an optical member having a light control part to reflect or absorb light in a predetermined wavelength and to pass through light in other than the predetermined wavelength in light in at least a visible light band, in accordance with a reproduction reference image for reproducing an original image, and a light transmissive part to pass through light in at least the visible light range including the predetermined wavelength.

Abstract: Apparatus with a rotationally drivable receptacle for a grinding tool to be tested, wherein the apparatus comprises: an optical testing apparatus arranged such that the grinding tool, while being rotatably driven, is at least partially irradiated by light emanating from an emitter of the testing apparatus, and that at least a portion of the light from the grinding tool is reflectable in the direction towards a sensor of the testing apparatus, wherein the sensor is adapted to provide test information, a computing device which is designed for processing the test information in order to determine a 3-dimensional vector model of the grinding tool from macroscopic basic information, a memory in which a target vector model is stored, a computing device which is designed for comparing the vector model with the target vector model in order to enable the determination of deviations between the vector models.

Abstract: The invention relates to a porous optical fiber for the detection of an analyte in a fluid by optical probing. The optical fiber has a first end and a second end opposite to the first end, as seen in a longitudinal direction, and a circumferential surface delimiting the optical fiber in radial directions perpendicular to the longitudinal direction. The optical fiber comprises a core adapted for supporting at least one optical mode propagating in the longitudinal direction, the core having a circumferential interface delimiting the core in the radial directions. The optical fiber further comprises pores penetrating from an opening at the circumferential surface through the circumferential interface into the core of the optical fiber, wherein a cross-sectional dimension of the openings is dimensioned so as to prevent a particulate fraction of the fluid from entering the pores, while allowing the analyte to enter the pores.

Abstract: Devices, systems and methods for widefield three-dimensional (3D) microscopy with a quantum entanglement light source are described. An example method includes generating a first set of photons and a second set of photons, wherein each of the photons in the first set is quantum entangled with a corresponding photon in the second set, directing the second set of photons toward a sample and simultaneously directing the first set of photons toward a first two-dimensional (2D) detector, detecting, from the sample, a plurality of photons at a second 2D detector, analyzing detections from the first and second 2D detectors to determine coincidence information, and determining one or more characteristics associated with at least a three-dimensional (3D) section of the sample based on collective detections at the first and the second 2D detectors.

Abstract: Highly sensitive assays for pathogen detection, identification and/or analysis including, but not limited to, sensing of metabolite patterns associated with high-risk drug resistance phenotypes.

Abstract: A method may include measuring a formation sample using a Raman spectrometer to determine a formation sample characteristic, wherein the formation sample characteristic is mineral ID and distribution, carbon ID and distribution, thermal maturity, rock texture, fossil characterization, or combinations thereof.

Abstract: A method for multi-target detection and an apparatus for multi-target detection are capable of detecting at least two targets in real time or near real time. The real-time detection or near real time detection can be achieved by at least one of a Recipe Group Approach, an End Member Grouping Approach, and a Pixelated Grouping Based Approach.

Abstract: A system and method for determining the pH of tissue in vivo. A Raman spectrometer is used to collect Raman spectra from the target tissue. The Raman spectra are baseline subtracted and assessed to determine the concentration of HPO4?2 and H2PO4?1 for the purposes of calculating the pH. The approach was validate in vitro using PBS solutions of known pH. The approach was confirmed in vivo using rat and swine models by probing the immediate vicinity of a contusive spinal cord injury (SCI) in the first minutes and hours after injury. Using a dynamic analysis and the Henderson-Hasselbalch equation, the average of (N=12) noninvasive Raman-based pH measurements of CSF was 7.073±0.156 and at >95% confidence there is no statistically significant difference between the Raman-based and the physically sampled results.

Abstract: The present invention relates to a system and method of skin detection of a human subject using a textile product. The textile product (10, 31, 43) is made from or comprising textile including near-infrared, NIR, absorbing pigments. It supports and/or partially covers the human subject while skin detection and/or detection/monitoring vital signs of the human subject is carried out. A increased contrast between the textile product and skin in the NIR wavelength range is thus achieved.

Abstract: The present invention relates to micro-/nanostructured compound-eye arrays and fabrication method thereof, and discloses a fabrication method and applications for the molded polymer parts with the micro-/nanostructured compound-eye arrays on their surfaces, which exhibit both hydrophobicity and light trapping. The fabrication method for the molded polymer parts with the micro-/nanostructured compound-eye arrays includes following steps.

Abstract: Technical solutions are described for implementing an optogenetics treatment using a probe and probe controller are described. A probe controller controls a probe to perform the method that includes emitting, by a light source of the probe, the probe is embeddable in a tissue, a light wave to interact with a corresponding chemical in one or more cells in the tissue. The method further includes capturing, by a sensor of the probe, a spectroscopy of the light wave interacting with the corresponding chemical. The method further includes sending, by the probe, the spectroscopy to an analysis system. The method further includes receiving, by the probe, from the analysis system, adjusted parameters for the light source, and adjusting, by a controller of the probe, settings of the light source according to the received adjusted parameters to emit a different light wave to interact with the corresponding chemical.

Abstract: A sensor configured to determine a physical state of a vehicle occupant within a vehicle includes an electrode containing nanoscale metal fibers. The electrode is located within the vehicle to be in contact with the vehicle occupant. The sensor also includes a controller configured to determine the physical state of the vehicle occupant based on an output of the electrode.

Abstract: A data concentration system for an inner detector of an oil-gas pipeline, and a timing control method, the system includes: a multi-channel data receiving module, a data concentration module and a data transmission module. The multi-channel data receiving module is electrically connected to a plurality of probes of an inner detector and is configured to receive detection data acquired by the plurality of probes. The data concentration module is electrically connected to the multi-channel data receiving module, and is configured to receive the detection data outputted by the multi-channel data receiving module and compress the detection data. The data transmission module is electrically connected to a data storage module of the inner detector and the data concentration module respectively, and the data transmission module is configured to receive compressed detection data.

Abstract: An improved method of sealing a window into an aperture in a body uses a lubricant comprising polymer particles suspended in a volatile, low viscosity, low surface tension carrier fluid. The carrier fluid is applied to one or both of the sidewalls of the window and aperture, and the window is pressed into the aperture such that the carrier fluid evaporates, leaving the polymer particles to fill interstitial surface voids, while enabling the sidewall of the window to make intimate mechanical contact with the sidewall of the aperture. While having broader application, the present disclosure finds particular utility in optical characterization techniques based upon the Raman effect and fluorescence probes used in process monitoring and control.

Abstract: Since the fat content of pork is a deciding factor in grading the quality of meat, the use of a noninvasive subcutaneous probe for real-time, in situ monitoring of the fat components is of importance to vendors and other interested parties. Fortunately, in situ, in vivo monitoring of subcutaneous fat can be accomplished with spatially offset Raman spectroscopy (SORS) using a fiber-optic probe. The probe acquires Raman spectra as a function of spatial offset. These spectra are used to determine the relative composition of fat-to-skin. The Raman intensity ratio varies disproportionately depending on the fat content, with variations in slope that are correlated to the thickness of the fat layer. Ordinary least square (OLS) regression using two components indicates that depth-resolved SORS spectra reflect the relative thickness of the fat layer.

Abstract: A method for detecting glutaraldehyde in a water sample in which Au-BSA nanoclusters have been introduced is provided. In the method, the water sample is mixed with the Au-BSA nanoclusters to form a mixture. The mixture is incubated for 2 to 10 minutes, and the glutaraldehyde present in the mixture reacts with the Au-BSA nanoclusters and causes fluorescence quenching of the Au-BSA nanoclusters. Then a fluorescence intensity of the quenched Au-BSA nanoclusters in the mixture is measured at an emission wavelength of 675 nm. A presence and concentration of glutaraldehyde in the water sample is then determined by comparing the measured fluorescence intensity of the quenched Au-BSA nanoclusters at the emission wavelength of 675 nm with fluorescence intensity values of calibration samples comprising Au-BSA nanoclusters and known glutaraldehyde concentrations.

Abstract: An object recognition apparatus includes a first spectrometer configured to obtain a first type of spectrum data from light scattered, emitted, or reflected from an object; a second spectrometer configured to obtain a second type of spectrum data from the light scattered, emitted, or reflected from the object, the second type of spectrum data being different from the first type of spectrum data; an image sensor configured to obtain image data of the object; and a processor configured to identify the object using data obtained from at least two from among the first spectrometer, the second spectrometer, and the image sensor and using at least two pattern recognition algorithms.

Abstract: Methods and systems for spectroscopy are provided. Exemplary methods include: illuminating, with a tunable laser, an analyte with first light; detecting, with a filtered sensor, a first Raman signal; illuminating, with the tunable laser, the analyte using second light; detecting, with the filtered sensor, a second Raman signal, the second Raman signal being shifted from the first Raman signal by a second predetermined increment; illuminating, with the tunable laser, the analyte using third light; detecting, with the filtered sensor, a third Raman signal, the third Raman signal being shifted from the second Raman signal by the second predetermined increment; constructing a Raman spectrum using the first Raman signal, the second Raman signal, and the third Raman signal; and determining at least one molecule of the analyte using the Raman spectrum and a database of predetermined Raman spectra.

Abstract: Methods, apparatus, and systems provide improved identification of selected biohazard and/or biohazard signatures from complex in vivo or in vitro samples and include deep UV native fluorescence spectroscopic analysis for multiple locations of a sample wherein classification results for individual locations are combined and spatially correlated to provide a positive or negative conclusion of biohazard signature presence (e.g., for signatures for viruses, bacteria, and diseases including SARS-CoV-2 and its variants and COVID-19 and its variants). Improvements include one or more of reduced sample processing time (minutes to fractions of a minute), reduced sampling cost (dollars to fractions of a dollar), high conclusion reliability (rivaling real time RT-PCR). Some embodiments may incorporate a stage or scanning mirror system to provide movement of a sample relative to an excitation exposure location. Some embodiments may incorporate Raman or phosphorescence spectroscopic analysis as well as imaging systems.