Viral Vaccine For Fish Patents (Class 424/817)
  • Patent number: 8420072
    Abstract: Vibrio vulnificus can cause infections in aquaculture-raised fish and is considered an opportunistic human pathogen. We isolated V. vulnificus from diseased hybrid tilapia (Oreochromis niloticus X O. aureus) cultured in a North American water reuse aquaculture facility. We have characterized the isolate using biochemical and molecular methods, developed a disease infection model, and determined that formalin-inactivated whole-cell vaccine provides protection against V. vulnificus. The V. vulnificus isolate was determined to be biotype 1, 16S rRNA type B, vcg type C, and vvhA type 2. Fish vaccinated with the formalin-inactivated whole-cell vaccine responded to vaccination as measured by agglutinating antibody titer. In two separate trials, vaccinated tilapia exhibited relative percent survival of 73 and 60% following challenge with the homologous isolate. In additional trials, vaccinated tilapia exhibited survival values of up to 87.5% following challenge with a heterologous isolate.
    Type: Grant
    Filed: March 24, 2011
    Date of Patent: April 16, 2013
    Assignee: The United States of America, as Represented by the Secretary of Agriculture
    Inventors: Craig A. Shoemaker, Benjamin R. LaFentz
  • Patent number: 7740864
    Abstract: Fish are immunized by a mass vaccination method, such as by immersion in water containing an attenuated strain of a pathogenic bacterium that does not effectively cause disease in fish when the non-attenuated pathogenic bacterium is exposed to the fish by immersion. An illustrative example of the method is for immunizing against coldwater disease caused by Flavobacterium psychrophilum, which may be attenuated by serial passage in media containing increasing amounts of an antibiotic, such as rifampicin.
    Type: Grant
    Filed: June 2, 2008
    Date of Patent: June 22, 2010
    Assignee: University of Idaho
    Inventors: Kenneth D. Cain, Benjamin R. LaFrentz, Scott LaPatra
  • Patent number: 6908616
    Abstract: The present invention relates to antigenic proteins derived from White Spot Syndrome virus having an estimated size of 19 kDa (VP 19) or 13 kDA (VP13), to the use of these proteins in vaccines and to vaccines on the basis of these proteins. Furthermore, the invention relates to antibodies against these proteins and to the use of antibodies in vaccines, to nucleic acid sequences encoding these proteins and to their use in vaccines. Also, the invention relates to the use of said proteins in the manufacture of a vaccine for prophylaxis and/or treatment of White Spot Syndrome in crustaceans, to vector vaccines and to diagnostic kits comprising said nuclei acids or antibodies.
    Type: Grant
    Filed: September 14, 2001
    Date of Patent: June 21, 2005
    Assignee: Akzo Nobel N.V.
    Inventors: Justinus Maria Vlak, Maria Cornelia Wilhelmina Van Hulten
  • Patent number: 6322793
    Abstract: An attenuated, avirulent recombinant vaccine providing challenged protection against channel catfish virus comprises deletion of gene 50. Gene 50 encodes a secreted glycoprotein. Removal of gene 50, or replacement of gene 50 with foreign genetic material, provides a vaccine with which induces virus specific immunity against CCV disease.
    Type: Grant
    Filed: June 16, 1999
    Date of Patent: November 27, 2001
    Assignee: Mississippi State University
    Inventors: Nathalie Vanderheijden, Joseph A. Martial, Larry A. Hanson
  • Patent number: 6180614
    Abstract: The present invention relates to methods of immunization of aquaculture species by introducing DNA expression systems into the aquaculture species. Such DNA expression systems preferably include DNA sequences encoding polypeptides of pathogens of species of aquaculture. The present invention also relates to methods of administration of DNA expression systems into aquaculture. Such methods include injection, spray, and immersion techniques. The methods of this invention are useful for prophylactic vaccination or therapeutic immunization of fin-fish, shellfish, or other aquatic animals against infectious diseases.
    Type: Grant
    Filed: July 14, 1998
    Date of Patent: January 30, 2001
    Assignee: Loeb Health Research Institute at The Ottawa Hospital
    Inventor: Heather L. Davis
  • Patent number: 5780448
    Abstract: The present invention relates to methods of immunization of aquaculture species by introducing DNA expression systems into the aquaculture species. Such DNA expression systems preferably include DNA sequences encoding polypeptides of pathogens of species of aquaculture. The present invention also relates to methods of administration of DNA expression systems into aquaculture. Such methods include injection, spray, and immersion techniques. The methods of this invention are useful for prophylactic vaccination or therapeutic immunization of fin-fish, shellfish, or other aquatic animals against infectious diseases.
    Type: Grant
    Filed: November 4, 1996
    Date of Patent: July 14, 1998
    Assignee: Ottawa Civic Hospital Loeb Research
    Inventor: Heather L. Davis
  • Patent number: 5525353
    Abstract: This invention relates broadly to a novel ambient-temperature process for making aquatic animal feed including fish and crustaceans and to the aquatic animal feed so produced.
    Type: Grant
    Filed: April 22, 1994
    Date of Patent: June 11, 1996
    Assignee: Aquacenter, Inc.
    Inventor: James R. Fajt
  • Patent number: 5354555
    Abstract: Subunit vaccines and their use for immunizing fish against infection by viruses are disclosed. In particular, plasmid pG8 is constructed by joining, with the plasmid pUC8, DNA which encodes the glycoprotein of infectious hematopoietic necrosis virus (IHNV). E. coli cells are transformed by pG8, whereby pure viral antigen is produced to provide a vaccine for the control of IHNV in fish.
    Type: Grant
    Filed: March 8, 1993
    Date of Patent: October 11, 1994
    Assignee: State of Oregon Acting By and Through The State Board of Higher Education on Behalf of Oregon State University
    Inventor: Jo-Ann C. Leong