Abstract: Provided herein, in some embodiments, are systems, methods, and compositions (e.g., cells and cell lysates) for enzymatically converting a polymeric glucose carbohydrate (e.g., starch) to sugar.

Abstract: The present invention provides methods for detecting viral infectivity and content in an enzyme preparation. In certain embodiments, the invention relates to methods for producing a pharmaceutical pancreatic enzyme composition. In additional embodiments, the invention relates to detecting infectious porcine parvovirus (PPV) and determining PPV content in pancreatic enzyme preparations (PEPs), including pancrelipase preparations.

Abstract: The invention is a method for reducing the viral and microbial content of biological extracts which contain solids. The method comprises the steps of, (1), providing a biological extract which contains solids that comprise a biologically active substance, selected from enzymes, proteins and peptides, or a mixture of such substances; and (2) subjecting the biological extract to a high-pressure treatment; wherein the biological activity of the biological extract after the high-pressure treatment is at least 50% of the biological activity of the biological extract before the high-pressure treatment.

Abstract: In order to prevent impairment of the pharmacological effect of pancreatine through added auxiliary substances or binding agents a pancreatine pellet consists exclusively of pancreatine.

Abstract: The disclosure relates to a Gram negative bacterial cell that is transformed with a nucleic acid molecule that encodes a Gram positive twin-arginine translocase and including methods for the production of polypeptides.

Abstract: Provided herein are compositions and methods that can remove, metabolize, or degrade a hydrocarbon in an area that is contaminated by hydrocarbons. Methods for bioremediation of an area such as an area of land, a body of water, or a shoreline that are contaminated by a hydrocarbon, such as from a crude oil spill are also described. The compositions and methods described herein can be used on natural flora and fauna as well as manmade materials that are contaminated by a hydrocarbon.

Abstract: The present invention relates to polynucleotides from Helobdella robusta, Laccaria bicolor, Lottia gigantea, Microcoleus chthonoplastes, Monosiga brevicollis, Mycosphaerella fijiensis, Mycospaerella graminicola, Naegleria gruberi, Nectria haematococca, Nematostella vectensis, Phycomyces blakesleeanus, Trichoderma resii, Physcomitrella patens, Postia placenta, Selaginella moellendorffii and Microdochium nivale, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention.

Abstract: A drift layer has a thickness direction throughout which a current flows and has an impurity concentration N1d for a first conductivity type. A body region is provided on a portion of the drift layer, has a channel to be switched by a gate electrode, has an impurity concentration N1b for the first conductivity type, and has an impurity concentration N2b for the second conductivity type greater than the impurity concentration N1b. A JFET region is disposed adjacent to the body region on the drift layer, has an impurity concentration N1j for the first conductivity type, and has an impurity concentration N2j for the second conductivity type smaller than the impurity concentration N1j. N1j?N2j>N1d and N2j<N2b are satisfied.

Abstract: The present invention describes the use of a genetically improved Trichoderma reesei strain allowing to limit microbiological contaminations during an industrial process. The genetic strain improvement allows the latter to overexpress an extracellular protein having known antimicrobial properties and compatible with the secretory system of strains of fungi such as Trichoderma reesei. This modified strain can be used to produce the cellulolytic and/or hemicellulolytic enzymes used in a method of producing ethanol from cellulosic or lignocellulosic materials referred to as “second generation” materials.

Abstract: Engineered multivalent and multispecific binding proteins that bind two different (e.g., nonoverlapping) epitopes of the same receptor or two different receptors expressed on the same cell are provided, along with methods of making and uses in the prevention, diagnosis, and/or treatment of disease.

Abstract: The fusion protein, especially recombinant, comprising domain (a) which is a functional fragment of soluble hTRAIL protein sequence beginning with an amino acid at a position not lower than hTRAIL95 or a sequence having at least 70% homology thereto; and domain (b) which is a sequence of pro-apoptotic effector peptide, wherein the sequence of domain (b) is attached at C-terminus and/or N-terminus of domain (a). The fusion protein has anticancer activity. The nucleotide sequence coding the fusion protein, expression vector and host cell for the preparation of the fusion protein, and the use of the fusion protein for treating cancer diseases.

Abstract: The invention relates to a method for producing pancreatin with reduced viral and microbial contamination, comprising the steps of (a) providing the pancreatin in solid form with a residual moisture of 0.5 weight % or less, down to almost zero, based on the pancreatin provided; (b) subjecting the pancreatin provided in step (a) to a heat treatment at a temperature of 84° C., preferably 80° C. and below; wherein, the biological activity of the pancreatin obtained in step (b) corresponds to at least 50% of the biological activity of the pancreatin provided in step (a); and the viral infectiousness of the pancreatin obtained in step (b) has been reduced by a factor of more than 1 log10 in comparison with the viral infectiousness of the pancreatin provided in step (a), as well as a pancreatin produced according to this method and its use for producing a medicine or a nutritional supplement.

Abstract: In order to prevent impairment of the pharmacological effect of pancreatine through added auxiliary substances or binding agents a pancreatine pellet consists exclusively of pancreatine.

Abstract: The invention relates to a method for producing pancreatin with reduced viral and microbial contamination, comprising the steps of (a) providing the pancreatin in solid form with a residual moisture of 0.5 weight % or less, down to almost zero, based on the pancreatin provided; (b) subjecting the pancreatin provided in step (a) to a heat treatment at a temperature of 84° C., preferably 80° C. and below; wherein, the biological activity of the pancreatin obtained in step (b) corresponds to at least 50% of the biological activity of the pancreatin provided in step (a); and the viral infectiousness of the pancreatin obtained in step (b) has been reduced by a factor of more than 1 log10 in comparison with the viral infectiousness of the pancreatin provided in step (a), as well as a pancreatin produced according to this method and its use for producing a medicine or a nutritional supplement.

Abstract: Processes for separating a viral load from a pancreatin sample and for quantitatively determining the viral load in a pancreatin sample with high sensitivity are described herein.

Abstract: An effective liquid preparation achieves high bioavailability (BA) of physiologically active peptides or proteins, including ghrelins, that are administered as drugs. Also provided is a method for improving the BA of physiologically active peptides or proteins, including ghrelins, that are subcutaneously injected in aqueous solutions. The liquid preparation contains: a physiologically active peptide or protein, such as ghrelins, as an active ingredient; an acid solution including one or a combination of two or more selected form the group consisting of acetic acid, lactic acid, phosphoric acid, glycine, citric acid, hydrochloric acid, propionic acid, butyric acid, benzoic acid and salts thereof; an alcohol; and a polar organic liquid including one or a combination of two or more selected from the group consisting of N-methyl-2-pyrrolidone, dimethylformamide, dimethylsulfoxide and methylparaben.

Abstract: The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.

Abstract: A new composite material for process application is described in this document. The composite material combine a regular base material (10) and small particles (14) using a layer of adhesive (12). The base material can be a sheet (10), a molded packing ring medium (16), or a structured packing medium. The material made of the base material can be plastics, ceramics, metals, porous materials, adsorptive materials, etc. The particles can be activated carbons, charcoals, anthracites, sands, and glass beads. The bound particles increase the surface roughness and the surface area of the base material by providing valleys (20) and crevices (22).

Abstract: The present invention relates to an advantageous and novel method of producing pancreatin which contains low amounts of residual organic solvent, and more particularly to a method which is more environmentally friendly than conventional methods of pancreatin production. More specifically, the present invention relates to a method which utilizes quantities of organic solvents which are about 10 times lower than that of conventional methods. A preferred method of pancreatin preparation, whereby a pancreatic past is autolysed, decontaminated, subjected to an ultrafiltration, dried and then defatted is herein disclosed. The present invention further relates to a pancreatin preparation which contains low amounts of residual solvent. This amount is about 15 to 30 times lower than pancreatin preparation obtained with these conventional methods.

Abstract: An insecticidal composition and its method of preparation are presented. The composition comprises a basal broth, a chitinase broth and a chemical fungicide. The basal broth has proteins hydrolyzed by papain and pancreatin while the chitinase broth is prepared by fermenting chitin and a chitinase-producing bacterial culture. The insecticidal composition has synergistic activity.

Abstract: A reagent composition for determining blood triglycerides comprising a color former and a thermostable lipoprotein lipase obtained from Streptomyces 7825 (FERM P-9983, FERM BP-2489) is presented.

Abstract: The present invention is directed toward a thermostable lipoprotein lipase capable of hydrolyzing triglycerides in lipoproteins to glycerol and fatty acids, a process for producing the thermostable lipoprotein lipase, and a blood triglyceride determining reagent containing the thermostable lipoprotein lipase. The thermostable lipoprotein lipase exhibits about 100% retention of the hydrolyzing activity when treated in a buffer having a pH of from bout 4 to 7 at about 60.degree. C. for about 15 minutes and a glycerol forming activity/fatty acid forming activity ratio of at least about 15%. The process comprises cultivating a thermophilic actinomycetes, particularly Streptomyces 7825 (FERM P-9983, FERM BP-2489 is cultivated to produce a thermostable lipoprotein lipase.

Abstract: Highly active pancreatin is obtained by autolysis of an aqueous isopropanol-containing tissue slurry, preferably buffered with sodium bicarbonate, until a test precipitation with aqueous isopropyl alcohol proves positive, and precipitating the batch with a higher concentration of isopropyl alcohol, resulting in a fiber suspension which can be sieved so that the solution obtained permits direct isolation of pancreatin by further increasing the concentration of isopropyl alcohol. A high bulk density of the finished dry preparation is achieved by stirring the precipitated pancreatin with isopropyl alcohol or acetone so as to bring the pancreatin to a concentration of 70-85% of isopropanol or 80-95% of acetone, isolating the pancreatin by suction filtration or centrifuging and drying it by treatment with dry air or nitrogen.

Abstract: A process for purification of crude kallikrein utilizing a specific affinity of kallikrein to p-aminobenzamidine, which comprises bringing a solution, containing kallikrein and contaminated by other undesirable enzymes and proteins, into contact with a water-insoluble carrier combined with p-aminobenzamidine, exclusively eluting out the other enzymes and proteins from the carrier by the use of a buffer solution of lower NaCl concentration and then exclusively eluting out kallikrein by the use of a buffer solution of higher NaCl concentration.