Abstract: A mushroom culture of Agaricus bisporus, comprising at least one set of chromosomes as a culture of line J14756-s3, includes a representative culture of the line, which has been deposited under NRRL Accession No. 67317. A method of producing a hybrid mushroom culture of Agaricus bisporus comprising: mating a culture comprising at least one set of chromosomes as a culture of line J14756-s3 with a second homokaryotic line culture. Additionally, mushrooms, parts of the culture and products incorporating the culture of the invention are provided.

Abstract: A method for at least one of protecting skin and promoting wound healing is provided. The method comprises administering to a subject in need an effective amount of Poria cocos extract, dehydropachymic acid (DPA), pachymic acid (PA), dehydrotumulosic acid (DTA), tumulosic acid (TA), polyporenic acid C (PAC), 3-epi-dehydrotumulosic acid (EDTA), dehydrotrametenolic acid (DTTA), trametenolic acid (TTA), dehydroeburicoic acid (DEA), eburicoic acid (EA), poricoic acid A (PAA) and/or poricoic acid B (PAB).

Abstract: Disclosed is a bioinsecticide formulation comprising highly resistant Beauveria bassiana microsclerotia and to its use for insect control, in particular for control of insects in grain silos, and to a method of preparation of said formulation. The microsclerotia are obtained in a culture medium comprising an iron (II) compound at a concentration of approximately 0.2 g/L.

Abstract: The present invention provides a method for making a consortium of fungi resistant to traces of carbamates contained in thiodicarb and to traces of synthetic pyrethroids contained in bifenthrin. During the course of this research, carried out over more than five years, the strains were subjected to a change in physical medium, adapting same to tolerate increasing concentrations of thiodicarb and bifenthrin. This compound enriches and increases the concentration of beneficial microorganisms, generating high-quality liquid biological fertilisers suitable for use in agricultural production, in land recovery and conservation, under the parameters established in sustainable organic farming, which seeks to conserve, recover and use nature or the environment without generating the least negative impact.

Abstract: A mycological biopolymer product consisting entirely of fungal mycelium is made by inoculating a nutritive substrate with a selected fungus in a sealed environment except for a void space, which space is subsequently filled with a network of undifferentiated fungal mycelium. The environmental conditions for producing the mycological biopolymer product, i.e. a high carbon dioxide (CO2) content (from 5% to 7% by volume) and an elevated temperature (from 85° F. to 95° F.), prevent full differentiation of the fungus into a mushroom. There are no stipe, cap, or spores produced. The biopolymer product grows into the void space of the tool, filling the space with an undifferentiated mycelium chitin-polymer, which is subsequently extracted from the substrate and dried.

Abstract: The present disclosure relates to methods of joining three or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro or in vivo. The method allows the joining of a large number of DNA fragments, in a deterministic fashion. It can be used to rapidly generate nucleic acid libraries that can be subsequently used in a variety of applications that include, for example, genome editing and pathway assembly. Kits for performing the method are also disclosed.

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

Abstract: Remote access methods and systems for plant pathogen (e.g., fungi or fungus-like organisms) assessment and management, and uses thereof, in particular for real-time agricultural applications, are described herein. In embodiments, the method and systems combine the capture of pathogenic spores (e.g., by impaction on an adhesive surface), laboratory analysis to identify the spores (e.g., by microscopy and/or PGR), collecting weather data, determining the level of risk for each pathogen, and providing an output to a user, who may for example access such risk data remotely as a risk report. A weather station is installed in proximity to the field/area where the spore sampling/collection occurs, and allows the constant transfer of weather data to effect the risk assessment. The risk assessment may be used to direct and optimize pesticide (e.g., fungicide) application in accordance with the pathogen identification and assessed risk level.

Abstract: Disclosed are examples of luminaires that provide light for general illumination and treat air via a biofilter. In the examples, a luminaire may include a light source configured to illuminate a space, a biofilter configured to treat air, and an air circulation system. The light source may be configured to illuminate a space in which the luminaire is located with general illumination light. The biofilter may include an air permeable membrane, a substrate, and a microorganism that treats air that comes in contact with the microorganism. The air circulation system is configured to draw air into contact with the biofilter and output air treated by contact with the biofilter into at least a portion of the space illuminated by the light source.

Abstract: The present invention provides compositions and methods for the multiplex detection of biomarkers in an environmental, non-biological or biological sample. Compositions and methods are provided for simultaneously detecting and identifying multiple pathogens, including viruses, bacteria, fungi, protozoa and helminths, present in a sample.

Abstract: A fungal growth structure comprising a nutritive vehicle, a porous material, an administrable space. fungal tissue comprising fungal hyphae having a growth pattern, the fungal tissue connecting said nutritive vehicle to said porous material to said administrable space, wherein the fungal tissue within said space defines at least one successive fungal material layer; and a chemically or physically altered separated portion of fungal material, the separated portion separated from said fungal tissue.

Abstract: The present invention relates to application of N(6)-(2-hydroxyethyl)-adenosine (HEA) and its derivatives as an adenosine A1 receptor agonist in preparation of drug or food, the HEA and its derivatives are used in treatment of diseases relating to adenosine receptor regulator, such as insomnia, pain, convulsion, apoplexia, Parkinson's disease, opioid drug addiction and kidney ischemia reperfusion injury etc. The present invention provides a new method for treatment of the diseases relating to nervous system and kidney.

Abstract: A crystalline pigment or colorant composition having high color intensity and/or low sugar content, and methods and processes of preparation. The composition may comprise purified fruit and/or vegetable color juices.

Abstract: A method for cultivating antrodia cinnamomea includes transplanting bacterial strains of antrodia cinnamomea into a petri dish receiving culture ingredients. The bacterial strains of antrodia cinnamomea are transferred into a culture container after mycelia of the bacterial strains of antrodia cinnamomea have grown to a predetermined area. The culture container receives a culture fluid. A formula composition of a culture medium for cultivating antrodia cinnamomea is added into the culture container after the mycelia of the bacterial strains of antrodia cinnamomea have grown in the culture container. The formula composition of the culture medium for cultivating antrodia cinnamomea includes growth basal ingredients and cultivating basal ingredients. The growth basal ingredients includes hydroxyhopanone.

Abstract: A growth medium formed as an inoculum including a preselected fungus and a nutrient material capable of being digested by the fungus is placed in or on a tool formed of a material capable of being at least partially consumed by the fungus. The tool may define a cavity of predetermined shape for the growth medium or the tool may form a scaffolding on which the growth medium grows into the final product taking on the shape of the tool.

Abstract: A paraffinic spray oil and a method of using the spray oil for controlling turfgrass pests is disclosed. The spray oil comprises paraffinic oil and a quick break emulsifier, which is formulated as an oil-in-water (O/W) emulsion for use. The paraffinic oil and emulsifier are present in a weight ratio ranging from about 95:5 to about 99.95:0.05, and preferably from about 98.5:1.5 to about 99.9:0.1. When applied to turfgrass, the O/W emulsion quickly releases the oil phase upon application to the turfgrass to contact pests thereon. When provided at sufficient paraffinic oil dosages, generally at least about 0.5 gal oil/acre and preferably in the range of about 0.5 gal/acre to about 60 gal/acre, the spray oil is effective in controlling a variety of turfgrass pests, particularly insect and fungal pests, with little or no phytotoxic effects. Further, use of the spray oil as indicated for controlling turfgrass pests also enhances the growth of turfgrass.

Abstract: An invention in the field of papermaking that relates in particular to sizing paper. Problems with state of the art methods are that the sizing chemicals used are typically expensive; may be available only in limited supply; are produced using methods that are damaging to the environment; and whose production is far from carbon-neutral. It is an object of the present invention to provide an alternative to the methods of the prior art and to overcome one or more of the above mentioned disadvantages.

Abstract: The present invention relates to Brassica plants comprising mutant FAD3 alleles, FAD3 nucleic acid sequences and proteins, as well as methods for generating and identifying said plants and alleles, which can be used to obtain seed oil with a reduced alpha-linolenic acid content.

Abstract: Described herein are compositions including filamentous fungal cells, such as Trichoderma fungal cells, having reduced protease activity and expressing fucosylation pathway. Further described herein are methods for producing a glycoprotein having fucosylated N-glycan, using genetically modified filamentous fungal cells, for example, Trichoderma fungal cells, as the expression system.

Abstract: The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

Abstract: The present invention relates to (E)-ethyl 2-(2-methyl-3-((2-(naphtho[2,1-b]furan-2-carbonyl)hydrazono)methyl)-1H-indol-1-yl)acetate as a novel tubulin polymerization inhibitor and a method for synthesizing the same. The compound of the present invention can inhibit mitosis and induce apoptosis and thus be used as an anticancer agent, by binding to tubulin to inhibit microtube polymerization. According to the synthesis method of the present invention, the reaction is simplified and the efficiency is 60% or higher, leading to a very high yield, thereby providing an effective synthesis method.

Abstract: A hybrid mushroom culture of Agaricus bisporus, designated as strain J11500, includes a representative culture of the strain, which has been deposited under NRRL Accession No. 50895. A method of producing a hybrid mushroom culture of Agaricus bisporus comprising: mating a homokaryotic line J10102-s69 with a homokaryotic line OWNC. Additionally, mushrooms, parts of the culture and products incorporating the culture are provided.

Abstract: The present invention provides a therapeutic agent for dementia comprising a pharmaceutically active substance derived from Hericium ramosum.

Abstract: A method for assisting an anti-cancer drug comprising administrating an effective amount of Antrodia camphorata fermentation solution to improve life quality of patient suffering from cancers after chemotherapy is provided.

Abstract: A concentration agent for microorganisms is provided that contains both lanthanum and carbonate. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.

Abstract: A method for the biological decomposition of organic compounds of the group of hydrocarbons, fats, oils, waxes, and derivatives thereof as well as mixtures thereof in media polluted therewith, in the presence of at least one dispersant, wherein the medium polluted with the pollutants is treated with at least one dispersant and with microorganisms.

Abstract: The present invention generally relates to compositions and methods of delivering substances in a dry mode, wherein the compositions include a surface layer disposed on the outer surface of the composition that is permeable to carbon dioxide and oxygen. The compositions may be used to deliver microorganisms to remove contaminates, such as oil, chemical, waste, or sewage, from soil, water, or air. In other embodiments, the compositions can also be used for delivering liquid food, liquid food additives, liquid biotech agricultural ingredients, conventional liquid agricultural ingredients, liquid human wellness and dietary supplements, and liquid fragrances and beauty products.

Abstract: A method and system for achieving a gas-liquid mass transfer includes delivering into a compartment of a container a liquid, the liquid having an exposed top surface disposed within the compartment. A stream of a gas is passed over the top surface of the liquid so that the stream of gas produces turbulence on the top surface that is sufficient to achieve the gas-liquid mass transfer. In one embodiment the liquid is a culture that includes cells or microorganisms and the mass transfer functions to oxygenate the culture sufficient to sustain the cells or microorganisms.

Abstract: A hybrid mushroom culture of Agaricus bisporus, designated as strain B14528, includes a representative culture of the strain, which has been deposited under NRRL Accession No. 50900. A method of producing a hybrid mushroom culture of Agaricus bisporus comprising: mating a homokaryotic line J12998-s39 with a homokaryotic line BW-s191. Additionally, mushrooms, parts of the culture and products incorporating the culture are provided.

Abstract: The present invention relates to methods of using meso-1,2,3,4-tetrahydroxybutane for the maintenance and/or improvement of biological cell function and activity, and for the prevention of improper cell functioning or cell death, in vitro, ex vivo, and in vivo over time and/or during exposure to stress. Meso-1,2,3,4-tetrahydroxybutane can be used to promote cell survival and as a cell protection agent, to increase cell viability, and to improve conversion of progenitor or stem cells to mature cells, whether in vitro, in vivo, ex-vivo, or transplanted.

Abstract: The composite material is comprised of a substrate of discrete particles and a network of interconnected mycelia cells bonding the discrete particles together. The mycelia cells are selected from the group consisting of at least one of Agrocybe brasiliensi, Flammulina velutipes, Hypholomoa capnoides, Hypholoma sublaterium, Morchella angusticeps, Macrolepiota procera and Coprinus comatus. The fungus digests the nutrient material over a period of time sufficient to grow hyphae and to allow the hyphae to form a network of interconnected mycelia cells through and around the discrete particles thereby bonding the discrete particles together to form a self-supporting composite material.

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

Abstract: The present invention relates to fungal cells and fungi which synthesize hyaluronan and to methods for preparing such fungi, and also to methods for preparing hyaluronan with the aid of these fungal cells or fungi. Furthermore, the present invention relates to the use of fungi for preparing hyaluronan and to food or feed which comprises hyaluronan.

Abstract: The invention provides isolated fungal endophytes and synthetic combinations thereof with host grass plants. Methods for inoculating grass plant with the endophytes, for propagating the grass-endophyte combinations, and for producing feeds and biofuels from grass-endophyte combinations are also provided.

Abstract: The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising: (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and (b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.

Abstract: A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises an amorphous metal silicate and that has a surface composition having a metal atom to silicon atom ratio of less than or equal to 0.5, as determined by X-ray photoelectron spectroscopy (XPS); (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device.

Abstract: The present invention provides a strain of entomopathogenic Beauveria bassiana, compositions comprising the entomopathogenic fungi strain or metabolites of the strain, and the use of the entomopathogenic fungi strain and compositions as biological control agents. Methods for the biological control of phytopathogenic insects using an entomopathogenic Beauveria bassiana fungi strain or one or more metabolites thereof, optionally together with other entomopathogenic fungi including fungi selected from strains of Lecanicillium spp., Paecilomyces fumosoroseus, and compositions comprising said fungi or metabolites thereof are also provided.

Abstract: A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA, a microbial cell containing a PUFA, and use of the microbial cell are provided. A method for producing a polyunsaturated fatty acid (PUFA) or a lipid containing a PUFA including culture of a microorganism capable of producing arachidonic acid (ARA) and/or dihomo-gamma-linolenic acid (DGLA) is provided, the method including at least one of the following steps: (a) adding an organic acid in an amount of 0.01 to 5 w/v % to a culture medium after the beginning of main culture; (b) increasing the pH of the culture medium to a range effective for culture after the beginning of the main culture; and (c) adding a metal sulfate in an amount of 0.01 to 0.5 w/w % to the main culture medium.

Abstract: The present invention relates to host cells having modified lipid-linked oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells have a GlcNAcMan3GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyl-transferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

Abstract: Provided herein are methods for transforming a Pyrococcus furiosus with a polynucleotide. In one embodiment, the method includes contacting a P. furiosus with a polynucleotide under conditions suitable for uptake of the polynucleotide by the P. furiosus, and identifying transformants at a frequency of, for instance, at least 103 transformants per microgram DNA. Also provided are isolated Pyrococcus furiosus having the characteristics of Pyrococcus furiosus COM1, and plasmids that include an origin of replication that functions in a Pyrococcus furiosus. The plasmid is stable in a recipient P. furiosus without selection for more than 100 generations and is structurally unchanged after replication in P. furiosus for more than 100 generations.

Abstract: The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as Pichia.

Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.

Abstract: High-yield antibiotics producing strain, preparation method and use thereof are provided in the present invention. The high-yield strain is a mutagenized strain derived from Colephoma empetri, and deposited in CGMCC with the accession number of CGMCC 4129. The preparation method comprises the following steps: (a) mixing a seed liquid of Colephoma empetri of Accession No. FERM BP-2635 with nitrosoguanidine to obtain a mixture a; (b) mixing said mixture a with a wall-breaking enzyme to obtain protoplasts; (c) regenerating said protoplasts to obtain single colonies; and (d) culturing said single colonies to obtain said mutagenized strain. The obtained strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable for industrialization.

Abstract: The present invention provides novel promoter and terminator sequences for use in gene expression in eukaryotic cells, such as algal cells. The invention further provides expression cassettes comprising a promoter, as described herein, operably linked to a gene. The invention further provides expression vectors and host eukaryotic cells, such as algal cells, for expressing a protein encoded by the gene; and methods for stably transforming eukaryotic algae such as Tetraselmis with transgenes.

Abstract: A process including: (a) mixing a microbial biomass, comprising oil-containing microbes, and at least one grinding agent capable of absorbing oil, to provide a disrupted biomass mix; (b) blending at least one binding agent with said disrupted biomass mix to provide a fixable mix capable of forming a solid pellet; and, (c) forming the solid pellet from the fixable mix. The process optionally includes extracting the solid pellet with a solvent to provide an extracted microbial oil.

Abstract: The present invention relates to a method for enhancing growth of plants which comprises contacting a Trichoderma strain with the plant or a plant seed under conditions effective for the Trichoderma strain to colonize the roots of the plant or a plant grown from the plant seed, thereby creating a plant-Trichoderma system. The plant or plant seed is grown under conditions effective to sustain the plant-Trichoderma system in a planting medium and to enhance plant growth, where the Trichoderma strain is selected from the group consisting of Trichoderma atroviride strain WW10TC4 (ATCC accession number PTA 9707), Trichoderma harzianum strain RR17Bc (ATCC accession number PTA 9708), Trichoderma harzianum strain F11Bab (ATCC accession number PTA 9709), and combinations thereof.

Abstract: The present invention relates to a method for enhancing growth of plants which comprises contacting a Trichoderma strain with the plant or a plant seed under conditions effective for the Trichoderma strain to colonize the roots of the plant or a plant grown from the plant seed, thereby creating a plant-Trichoderma system. The plant or plant seed is grown under conditions effective to sustain the plant-Trichoderma system in a planting medium and to enhance plant growth, where the Trichoderma strain is selected from the group consisting of Trichoderma atroviride strain WW10TC4 (ATCC accession number PTA 9707), Trichoderma harzianum strain RR17Bc (ATCC accession number PTA 9708), Trichoderma harzianum strain F11Bab (ATCC accession number PTA 9709), and combinations thereof.

Abstract: The present invention relates to a fertilizer comprising at least one microbe selected from the group consisting of Trichoderma viride (NRRL B-50520), Scopulariopsis brevicaulis (NRRL B-50521), Scopulariopsis brevicaulis (NRRL B-50522), and combinations thereof, and a substrate which is acted upon by the at least one microbe to release nitrogen. The invention also relates to a fertilizer comprising at least one microbe selected from a keratin degrading microorganism and a keratin substrate which is acted upon by the at least one microbe to release nitrogen. The invention further relates to a method for enhancing growth of plants with the fertilizer, and methods of making a fertilizer. The invention further relates to an isolated Trichoderma viride strain (NRRL B-50520) and two isolated Scopulariopsis brevicaulis strains (NRRL B-50521, NRRL B-50522).

Abstract: A fat-soluble fraction extracted from the fruiting body of Hericium erinaceum is demonstrated to inhibit the neuronal toxicity of amyloid beta-peptide (A?) and induce the synthesis of nerve growth factor (NGF), and has great potential as an active ingredient for pharmaceutical products, health food products, food products and/or beverages to prevent and/or treat dementia, especially Alzheimer-type dementia. This invention is to provide the bioactive fraction and its preparation method.