Medium Contains A Colony Stimulating Factor Patents (Class 435/385)
  • Patent number: 8975072
    Abstract: Provided are: a method for producing an immortalized human erythroid progenitor cell line, enabling efficient and stable production of enucleated red blood cells; and a method for producing human enucleated red blood cells from a human erythroid progenitor cell line obtained by the aforementioned production method. An expression cassette capable of inducing expression of HPV-E6/E7 genes in the presence of DOX was introduced into the genomic DNA of blood stem cells. Then, the blood stem cells were cultured in the presence of DOX and a blood growth factor. Thereby, immortalized cell lines of human erythroid progenitor cells were established. Further, it was revealed that culturing the cell lines under a condition where the expression of the HPV-E6/E7 genes was not induced enabled differentiation induction into enucleated red blood cells at a high ratio.
    Type: Grant
    Filed: January 18, 2013
    Date of Patent: March 10, 2015
    Assignee: Riken
    Inventors: Yukio Nakamura, Ryo Kurita
  • Patent number: 8895302
    Abstract: Methods of efficiently converting primate pluripotent stem cells to GABA neurons or cholinergic neurons, as well as applications thereof, are disclosed.
    Type: Grant
    Filed: August 10, 2011
    Date of Patent: November 25, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Su-Chun Zhang, Yan Liu, Lixiang Ma
  • Patent number: 8846395
    Abstract: A method for efficient generation of neutrophils, eosinophils, macrophages, osteoclasts, dendritic cells an Langerhans cells from human embryonic stem cells is disclosed.
    Type: Grant
    Filed: September 24, 2009
    Date of Patent: September 30, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Igor I. Slukvin, Kyung-Dal Choi, Maksym A. Vodyanyk
  • Patent number: 8735156
    Abstract: A spermatogonial stem cell line that is derived from testes of rats characterized by a desirable genetic background can serve as a source for cells to transplant into male-sterile recipient animals that are immuno-compatible with the spermatogonial line. Rat cells thus transplanted readily develop into fertilization-competent, haploid male gametes, with little or no endogenous sperm competition generated by the testes of the male-sterile recipient. This approach, constituting the first vector system for the use of rat spermatogonial lines from in vitro culture in generating mutant rats on a desired genetic background, effects maximal germline transmission of donor haplotypes from the transplanted spermatogonial cells.
    Type: Grant
    Filed: December 1, 2009
    Date of Patent: May 27, 2014
    Inventor: Franklin Kent Hamra
  • Patent number: 8669106
    Abstract: The invention provides, among other things, methods and systems for expanding CD133+ cells. The invention further provides methods and systems for increasing the blood flow to an ischemic tissue in a subject in need thereof, such as to ischemic myocardium. The invention further provides methods and systems for directing differentiation of expanded CD133+ cells. The invention further provides methods and systems for treating a subject with differentiated cells in a subject in need thereof.
    Type: Grant
    Filed: January 9, 2009
    Date of Patent: March 11, 2014
    Assignees: Arteriocyte Inc., Universite Pierre ET Marie Curie (Paris VI)
    Inventors: Ramasamy Sakthivel, Donald J. Brown, Hai-Quan Mao, Luc Douay, Vincent J. Pompili, Kevin McIntosh, Hiranmoy Das, Yukang Zhao
  • Patent number: 8586360
    Abstract: Provided herein are methods of producing erythrocytes from hematopoietic cells, particularly hematopoietic cells from placental perfusate in combination with hematopoietic cells from umbilical cord blood, wherein the method results in accelerated expansion and differentiation of the hematopoietic cells to more efficiently produce administrable erythrocytes. Further provided herein is a bioreactor in which hematopoietic cell expansion and differentiation takes place.
    Type: Grant
    Filed: July 1, 2010
    Date of Patent: November 19, 2013
    Assignee: Anthrogenesis Corporation
    Inventors: Stewart Abbot, Lin Kang, Vanessa Voskinarian-Berse, Xiaokui Zhang
  • Patent number: 8569060
    Abstract: An in vitro or ex vivo method of producing a population of lineage committed haematopoietic progenitor or mature haematopoietic cells other than cells of the neutrophil lineage, including the steps of providing a population of haematopoietic progenitor cells; and culturing the haematopoietic progenitor cells in an animal cell culture medium including one or more cytokines that differentiate the haematopoietic progenitor cells into lineage committed haematopoietic progenitor and/or mature haematopoietic cells, under static conditions until the cells are at a cell density at which oxygen transfer via the surface of the culture medium is insufficient for growth of the progenitor cells and progeny thereof under static conditions, and then agitating the culture medium to produce a population of lineage committed haematopoietic progenitor or mature haematopoietic cells other than cells of the neutrophil lineage.
    Type: Grant
    Filed: January 8, 2009
    Date of Patent: October 29, 2013
    Assignee: The University of Queensland
    Inventors: Nicholas Eion Timmins, Lars Keld Nielsen, Emma Louise Palfreyman
  • Patent number: 8435785
    Abstract: This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.
    Type: Grant
    Filed: February 1, 2012
    Date of Patent: May 7, 2013
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Igor I. Slukvin, James A. Thomson, Maksym A. Vodyanyk, Maryna E. Gumenyuk
  • Patent number: 8431354
    Abstract: RP-factors, their cognate receptors, convertases, respective genes and inhibitors or mimetics thereof are described. In particular, antibodies, pharmaceutical compositions and (therapeutic, diagnostic) methods based on the RP-factors and their receptors/convertases are described.
    Type: Grant
    Filed: June 3, 1998
    Date of Patent: April 30, 2013
    Assignee: Aberystwyth University
    Inventors: Galina V Mukamolova, Arseny S Kaprelyants, Danielle I Young, Douglas B Kell, Michael Young
  • Patent number: 8415157
    Abstract: A cell culture chamber and a cell culture method that are capable of effectively constructing an intercellular network in a culture space are provided. A cell culture chamber (10)according to the present invention is a cell culture chamber (10)including a plurality of microchambers (11) formed on a surface thereof, characterized in that convex portions (side walls 12) that partition the microchambers (11) adjacent to each other are formed to prevent cells from being adhered to upper surfaces of the convex portions. Consequently, cells can be cultured exclusively within the microchambers (11), and an intercellular network can be constructed effectively.
    Type: Grant
    Filed: September 9, 2011
    Date of Patent: April 9, 2013
    Assignee: KURARAY Co., Ltd.
    Inventors: Go Tazaki, Tomoko Kosaka, Motohiro Fukuda
  • Patent number: 8252583
    Abstract: A method for inducing differentiation of ES cells into cardiomyocytes, which comprises contacting the ES cells with an agonist for G-CSF receptor.
    Type: Grant
    Filed: June 6, 2008
    Date of Patent: August 28, 2012
    Assignee: Keiichi Fukuda
    Inventors: Keiichi Fukuda, Shinsuke Yuasa, Kenichiro Shimoji
  • Patent number: 8241621
    Abstract: Disclosed are cells, methods of modulating cells, and therapeutic uses of the cells for the immune modulation of mammals in need thereof. Immune modulation including alteration of cytokine profile, cytotoxic activity, antibody production and inflammatory states is achieved through the administration of various cell types that have been unmanipulated or manipulated in order to endow specific biological activity. Cellular subsets and administration of the subsets in combination with various agents are also provided. One embodiment teaches the previously unknown finding that adipose tissue derived mononuclear cells contain T cells with immune regulatory properties that alone or synergistically with various stem cells induce immune modulation upon administration. Another embodiment is the finding that stimulation of stem cell activation results in stem cell secondary activation of immune modulatory cells, one type which is T regulatory cells (Tregs).
    Type: Grant
    Filed: December 18, 2007
    Date of Patent: August 14, 2012
    Assignee: Medistem Laboratories
    Inventor: Thomas E. Ichim
  • Patent number: 8202724
    Abstract: A method of ex-vivo expanding a population of stem cells, while at the same time inhibiting differentiation of the stem cells. The method comprises ex-vivo providing the stem cells with conditions for cell proliferation and with at least one copper chelator in an amount and for a time period for permitting the stem cells to proliferate and, at the same time, for reducing a capacity of the stem cells to differentiate.
    Type: Grant
    Filed: October 6, 2010
    Date of Patent: June 19, 2012
    Assignees: Gamida Cell Ltd., Hadasit Medical Research Services and Development Ltd.
    Inventors: Tony Peled, Eitan Fibach, Avi Treves
  • Patent number: 8183033
    Abstract: The invention relates to methods for preparing and performing quantitative PCR analyses, a new sealing device and a new use. According to the invention, a sample vessel containing the samples to be analyzed is sealed by placing a planar sealing device on the vessel to cover the samples and applying pressure on the sealing device in order to deform the sealing device so as to form a light-refracting geometry individually for the samples to be analyzed. The invention offers a convenient way of sealing the vessel and forming analysis-improving optical lenses over the samples simultaneously.
    Type: Grant
    Filed: March 21, 2008
    Date of Patent: May 22, 2012
    Assignee: Bioinnovations Oy
    Inventor: Bruce R Turner
  • Patent number: 8173427
    Abstract: A method is provided for producing a population of post-mitotic cells of the neutrophil lineage, which method comprises the ex vivo steps of: (a) providing a population of cells comprising neutrophil progenitor cells; and (b) culturing the population of cells in an animal cell culture medium comprising (i) one or more early acting cytokines and (ii) one or more cytokines that differentiate said progenitor cells into a neutrophil specific lineage, under conditions of low oxidative stress, the culture medium being agitated when the cells are at a cell density at which oxygen transfer via the surface of the culture medium is insufficient for growth of the progenitor cells and the progeny thereof under static conditions, to produce a population of post-mitotic cells of the neutrophil lineage. The resulting population of cells can be used to increase the number of neutrophils in a patient.
    Type: Grant
    Filed: July 23, 2007
    Date of Patent: May 8, 2012
    Assignee: The University of Queensland
    Inventors: Lars K. Nielsen, Emma L. Palfreyman, Nicholas E. Timmins
  • Patent number: 8133732
    Abstract: This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.
    Type: Grant
    Filed: September 7, 2010
    Date of Patent: March 13, 2012
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Igor I. Slukvin, James A. Thomson, Maksym A. Vodyanyk, Maryna E. Gumenyuk
  • Patent number: 7993917
    Abstract: Methods for the diagnosis of visceral, cutaneous and canine leishmaniasis in a subject suspected of being infected with the parasitic protozoa Leishmania is disclosed. Disclosed are antibody-capture enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies to Leishmania parasite soluble antigens and antigen-capture ELISAs for the detection of Leishmania parasite soluble antigens in host samples. Also disclosed are immunodiagnostic kits for the detection of Leishmania parasite circulating antigens or IgM and IgG antibodies in a sample from subject having visceral, cutaneous or canine leishmaniasis. In these methods and kits, detection may be done photometrically or visually. The methods and kits also allow the visualization of Leishmania amastigotes or promastigotes in a sample.
    Type: Grant
    Filed: August 20, 2009
    Date of Patent: August 9, 2011
    Assignee: The United States of America as represented by the Secretary of the Army
    Inventor: Samuel K. Martin
  • Patent number: 7989178
    Abstract: A system combining a clonogenic differentiation assay with an instrument-based ATP bioluminescence proliferation assay to produce a standardized colony-forming stem and progenitor cell potency assay is provided.
    Type: Grant
    Filed: June 6, 2008
    Date of Patent: August 2, 2011
    Assignee: Hemogenix, Inc.
    Inventor: Ivan N. Rich
  • Patent number: 7923247
    Abstract: A method for producing proliferating cultures of dendritic cell precursors is provided. Also provided is a method for producing mature dendritic cells in culture from the proliferating dendritic cell precursors. The cultures of mature dendritic cells provide an effective means of producing novel T cell dependent antigens comprised of dendritic cell modified antigens or dendritic cells pulsed with antigen, including particulates, which antigen is processed and expressed on the antigen-activated dendritic cell. The novel antigens of the invention may be used as immunogens for vaccines or for the treatment of disease. These antigens may also be used to treat autoimmune diseases such as juvenile diabetes and multiple sclerosis.
    Type: Grant
    Filed: July 17, 2008
    Date of Patent: April 12, 2011
    Assignees: Argos Therapeutics, Inc., The Rockefeller University
    Inventors: Ralph M. Steinman, Kayo Inaba, Gerold Schuler
  • Patent number: 7883861
    Abstract: The present invention relates generally to kits that provide reagent mixes and instructions for the use thereof, in performing high-throughput assay methods that determine the proliferative status of isolated target cell populations. The methods measure the luminescent output derived from the intracellular ATP content of incubated target cells, and correlate the luminescence with the proliferative status of the cells. The present invention further relates to kits that provide reagent mixes and instructions for high-throughput assays methods for screening compounds that may modulate the proliferative status of a target cell population. The kits of the present invention and methods therein described may be used for determining the proliferative status of any isolated cell line or type. The kits and methods of the present invention address the need for rapid assays that determine the proliferative status of isolated hematopoietic stem and progenitor cells and of subpopulations of differentiated cells thereof.
    Type: Grant
    Filed: March 17, 2008
    Date of Patent: February 8, 2011
    Assignee: HemoGenix, Inc.
    Inventor: Ivan N. Rich
  • Patent number: 7855076
    Abstract: The disclosure provides methods of modulating the activity of DDR1. Methods for screening for agents that activate DDR1 are disclosed. Methods for inducing the maturation of immature macrophages and immature dendritic cells are also disclosed. In addition, methods for increasing neutrophil activation using a DDR1 activating agent, and methods for increasing leukocyte migration using a DDR1 activating agent, are provided.
    Type: Grant
    Filed: December 11, 2002
    Date of Patent: December 21, 2010
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: Teizo Yoshimura, Hidenobu Kamohara
  • Publication number: 20100298217
    Abstract: Provided are mutant class III receptor tyrosine kinases (RTKIII) comprising a mutation at the amino acid residue corresponding to the conserved cysteine at residue 432 (C432) or 439 (C439) of a mouse colony-stimulating factor (1) receptor (CSF-IR) precursor having the amino acid sequence of SEQ ID NO: 1, where the mutation is a replacement of the cysteine with an amino acid having an uncharged polar R group. Also provided are mutant RTKIIIs comprising a tyrosine to phenylalanine mutation. Additionally provided are extracellular domains of the above-identified mutant RTKIIIs comprising cysteine to serine mutations. Further provided are stable cell lines of macrophages lacking a native CSF-IR. Also provided are isolated nucleic acids encoding any of the above-described mutant RTKIIIs or extracellular domains. Vectors comprising those nucleic acids are also provided. Additionally provided are methods of preparing the above-identified stable cell lines.
    Type: Application
    Filed: May 22, 2008
    Publication date: November 25, 2010
    Inventors: Evan Richard Stanley, Ying Xiong
  • Patent number: 7811821
    Abstract: This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.
    Type: Grant
    Filed: May 31, 2006
    Date of Patent: October 12, 2010
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Igor I. Slukvin, James A. Thomson, Maksym A. Vodyanyk, Maryna E. Gumenyuk
  • Patent number: 7709258
    Abstract: The present invention relates generally to high-throughput assay methods that determine the proliferative status of hematopoietic stem and progenitor cells. The present invention further relates to high-throughput assays for screening compounds that modulate the growth of hematopoietic stem and progenitor cells and for identifying subpopulations thereof that are suitable for transplantation. The assay of the present invention is particularly useful for quality control and monitoring of the growth potential in the stem cell transplant setting and would provide improved control over the reconstitution phase of transplanted cells.
    Type: Grant
    Filed: March 17, 2008
    Date of Patent: May 4, 2010
    Assignee: HemoGenix, Inc.
    Inventor: Ivan N. Rich
  • Patent number: 7704737
    Abstract: This invention relates to methods of producing oligodendrocytes from multipotent neural stem cells by using at least one oligodendrocyte promoting factor, particularly granulocyte-macrophage colony stimulating factor, granulocyte colony stimulating factor, interleukin 3 or interleukin 5. The neural stem cells may optionally be expanded prior to being subjected to the oligodendrocyte promoting factor.
    Type: Grant
    Filed: July 30, 2003
    Date of Patent: April 27, 2010
    Assignee: Stem Cell Therapeutics Inc.
    Inventor: Samuel Weiss
  • Patent number: 7700354
    Abstract: The present invention relates generally to high-throughput assay methods that determine the proliferative status of hematopoietic stem and progenitor cells. The present invention further relates to high-throughput assays for screening compounds that modulate the growth of hematopoietic stem and progenitor cells and for identifying subpopulations thereof that are suitable for transplantation. The assay of the present invention is particularly useful for quality control and monitoring of the growth potential in the stem cell transplant setting and would provide improved control over the reconstitution phase of transplanted cells.
    Type: Grant
    Filed: March 17, 2008
    Date of Patent: April 20, 2010
    Assignee: HemoGenix, Inc.
    Inventor: Ivan N. Rich
  • Patent number: 7695426
    Abstract: Methods are provided for enhancing viability comprising administering at least one inhibitor of p53 or a p53-associated pathway to one or more of the following: the embryo, oocytes, sperm, a femme animal or a male animal.
    Type: Grant
    Filed: August 20, 2004
    Date of Patent: April 13, 2010
    Assignee: Biological Resources Pty Ltd
    Inventor: Christopher O'Neill
  • Patent number: 7666615
    Abstract: The present invention relates generally to assays, methods, and kits that provide reagent mixes and instructions for determining the proliferative status of isolated target cell populations. The methods measure the luminescent output derived from the intracellular ATP content of incubated target cells, and correlate the luminescence with the proliferative status of the cells.
    Type: Grant
    Filed: November 17, 2006
    Date of Patent: February 23, 2010
    Assignee: HemoGenix, Inc.
    Inventor: Ivan N. Rich
  • Publication number: 20100041147
    Abstract: Disclosed and claimed are methods for the isolation and use of stem cell inhibiting factors for regulating the abnormal stem cell cycle and for accelerating the post-chemotherapy peripheral blood cell recovery. Also disclosed and claimed are the inhibitors of stem cell proliferation.
    Type: Application
    Filed: August 3, 2009
    Publication date: February 18, 2010
    Applicant: WELLSTAT THERAPEUTICS CORPORATION
    Inventors: Irena Tsyrlova, Stephen D. Wolpe
  • Patent number: 7659119
    Abstract: We describe an improved method for generating sizable numbers of mature dendritic cells from nonproliferating progenitors in human blood. The first step or “priming” phase is a culture of T cell depleted mononuclear cells in medium supplemented with GM-CSF and IL-4 to produce immature dendritic cells. The second step or “differentiation” phase requires the exposure to dendritic cell maturation factor such as monocyte conditioned medium. Using this two-step approach, substantial yields are obtained. The dendritic cells derive from this method have all the features of mature cells. They include a stellate cell shape, nonadherence to plastic, and very strong T cell stimulatory activity. The mature dendritic cells produced according to this invention are useful for activating T cells.
    Type: Grant
    Filed: February 12, 1996
    Date of Patent: February 9, 2010
    Assignees: Argos Therapeutics, Inc., The Rockefeller University
    Inventors: Ralph M. Steinman, Nina Bhardwaj, Gerold Schuler
  • Patent number: 7632674
    Abstract: The present invention provides an apparatus for stimulating an animal cell and recording its physiological signal and methods of making and using thereof. The purpose of the present invention is to provide an apparatus for stimulating an animal cell and recording its physiological signal that is efficient, convenient, and accurate. The apparatus for stimulating an animal cell and recording its physiological signal of the present invention comprises a poor conductive substrate, wherein on at least one surface of the substrate is provided at least one unit conductive polymer layer and at least one good conductive microelectrode.
    Type: Grant
    Filed: November 29, 2002
    Date of Patent: December 15, 2009
    Assignees: CapitalBiochip Corporation, Tsinghua University
    Inventors: Wanli Xing, Zhe Yu, Guangxin Xiang, Liangbin Pan, Jing Cheng
  • Patent number: 7611896
    Abstract: Methods for the diagnosis of visceral, cutaneous and canine leishmaniasis in a subject suspected of being infected with the parasitic protozoa Leishmania is disclosed. Disclosed are antibody-capture enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies to Leishmania parasite soluble antigens and antigen-capture ELISAs for the detection of Leishmania parasite soluble antigens in host samples. Also disclosed are immunodiagnostic kits for the detection of Leishmania parasite circulating antigens or IgM and IgG antibodies in a sample from subject having visceral, cutaneous or canine leishmaniasis. In these methods and kits, detection may be done photometrically or visually. The methods and kits also allow the visualization of Leishmania amastigotes or promastigotes in a sample.
    Type: Grant
    Filed: June 13, 2005
    Date of Patent: November 3, 2009
    Assignee: The United States of America as represented by the Secretary of the Army
    Inventor: Samuel K. Martin
  • Patent number: 7566568
    Abstract: The present invention relates to a process for deriving dendritic cells from mononuclear cells in culture comprising the step of putting in contact type I IFN with said mononuclear cells. Dendritic cells suitable as cellular adjuvants in prophylactic as well as therapeutic vaccination of animal and human beings, are obtainable thereby, after a single step treatment in a brief period of time. Dendritic cells obtainable thereby, pharmaceutical compositions including them, in particular a vaccine comprising said cells as active principle, and a method of treatment of a pathology associated with the presence of an antigen in human beings, are further objects of the invention, as well as a kit for deriving said dendritic cells and a method for the ex vivo expansion of T cells using them.
    Type: Grant
    Filed: April 27, 2001
    Date of Patent: July 28, 2009
    Assignee: Istituto Superiore di Sanita
    Inventors: Filippo Belardelli, Stefano Maria Santini, Stefania Parlato, Tiziana Di Pucchio, Mariantonia Logozzi, Caterina Lapenta, Maria Ferrantini, Laura Santodonato, Giuseppina D'Agostino
  • Publication number: 20090148405
    Abstract: A method of inducing dendritic cell (DC) development by administering Macrophage-Colony Stimulating Factor Receptor Ligand is provided. M-CSF receptor ligands induce DCs to differentiate into subtypes, for example plasmacytoid DCs and conventional DCs. Induction with an M-CSF receptor ligand can be achieved in vitro from hematopoietic precursors, such as bone marrow cells, or in vivo. In vitro, M-CSF receptor ligand-derived DCs can be used to produce cytokines and to stimulate other immune response cells. M-CSF receptor ligands can also be used to induce precursor cells removed from an animal to develop into DCs. In addition, these isolated DCs can be exposed to antigens to stimulate a specific immune response when reintroduced into the animal. Treatments for cancers, such as Acute Myeloid Leukemia, and autoimmune diseases such as Systemic Lupus Erythematosus, are also provided in the invention.
    Type: Application
    Filed: December 22, 2008
    Publication date: June 11, 2009
    Inventors: Hubertus Hochrein, Meredith O'Keeffe
  • Publication number: 20090028838
    Abstract: The present invention provides a serum-free supplement which supports the growth of hematopoietic cells in culture. Also provided are a medium comprising a basal medium supplemented with the serum-free supplement of the present invention. The present invention also provides methods for culturing and for differentiating hematopoietic cells.
    Type: Application
    Filed: August 15, 2008
    Publication date: January 29, 2009
    Applicant: INVITROGEN CORPORATION
    Inventors: John P. Daley, Barbara M. Dadey, William C. Biddle, Michelle G. Wysocki
  • Patent number: 7455983
    Abstract: This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of mouse embryonic fibroblast feeder cells to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by defined components added to the culture environment that support rapid proliferation without differentiation. The medium contains an isotonic buffer, a blend of essential nutrients such as protein and lipids, and an effective growth factor or combination of factors that promote proliferation while inhibiting differentiation. Culturing human embryonic stem cells in fresh medium on an extracellular matrix according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers.
    Type: Grant
    Filed: September 24, 2004
    Date of Patent: November 25, 2008
    Assignee: Geron Corporation
    Inventors: Chunhui Xu, Yan Li, Ramkumar Mandalam
  • Publication number: 20080274125
    Abstract: The present invention concerns the field of biology and virology. In particular, the invention concerns a method for obtaining human cell lines, in particular human stem cells derived from human embryonic stem cells, the method comprising separation from the serum, the feeder layer and at least one growth factor. The cell lines are capable of proliferating indefinitely in a basic culture medium. The invention also concerns the use of the cells derived from such cell lines for virus replication, and more particularly for producing human or veterinary vaccines, as well as for producing recombinant proteins of therapeutic interest.
    Type: Application
    Filed: December 8, 2005
    Publication date: November 6, 2008
    Applicant: VIVALIS
    Inventor: Fabienne Guehenneux
  • Patent number: 7426440
    Abstract: A method of scoring the biological activity of bioactive agents like colostrum comprising conducting separate bioassays for cell restitution and cell proliferation on the bioactive agent, a comparative growth factor stimulating agent, and (preferably) a negative baseline control sample containing the cells and growth medium alone. These values are then plugged into the equations provided in this application to calculate a Restitution Score (“RS”), Proliferation Score (“PS”), and a composite repair and protection factor score (“RPF”). In this manner, the bioactivity of compositions that enhance the repair and/or proliferation of mammalian cells, or act as additives to growth media used to maintain and grow laboratory cell cultures can be quickly and reproducibly obtained. This invention may be applied to a wide variety of bioactive agents used to treat a large assortment of functional cells in organ tissues.
    Type: Grant
    Filed: September 24, 2004
    Date of Patent: September 16, 2008
    Assignee: Nutritional Bioscience Ltd.
    Inventor: Raymond J. Playford
  • Patent number: 7390660
    Abstract: The invention relates to a method for reducing glucose consumption during cultivation of animal cells, which comprises cultivating animal cells in the presence of a bi- or tricarbonic acid or a salt thereof at a concentration of about 1 to 50 mmol/l.
    Type: Grant
    Filed: March 3, 2003
    Date of Patent: June 24, 2008
    Assignee: Hoffman-La Roche Inc.
    Inventors: Ulrich Behrendt, Horst Eberhardt, Berthold Szperalski
  • Patent number: 7374934
    Abstract: The present invention relates to novel immortalized precursor cell populations derived from embryonic stem cell populations and methods to produce such cell populations. Also disclosed is an assay to identify regulatory compounds capable of controlling cell growth for therapeutic and experimental use.
    Type: Grant
    Filed: August 13, 2002
    Date of Patent: May 20, 2008
    Assignee: National Jewish Medical and Research Center
    Inventors: Gordon M. Keller, Robert G. Hawley, Kyunghee Choi
  • Patent number: 7354729
    Abstract: The present invention relates generally to high-throughput assay methods that determine the proliferative status of hematopoietic stem and progenitor cells. The present invention further relates to high-throughput assays for screening compounds that modulate the growth of hematopoietic stem and progenitor cells and for identifying subpopulations thereof that are suitable for transplantation. The assay of the present invention is particularly useful for quality control and monitoring of the growth potential in the stem cell transplant setting and would provide improved control over the reconstitution phase of transplanted cells.
    Type: Grant
    Filed: January 29, 2002
    Date of Patent: April 8, 2008
    Assignee: HemoGenix, Inc.
    Inventor: Ivan N. Rich
  • Patent number: 7354730
    Abstract: The present invention relates generally to kits that provide reagent mixes and instructions for the use thereof, in performing high-throughput assay methods that determine the proliferative status of isolated target cell populations. The methods measure the luminescent output derived from the intracellular ATP content of incubated target cells, and correlate the luminescence with the proliferative status of the cells. The present invention further relates to kits that provide reagent mixes and instructions for high-throughput assays methods for screening compounds that may modulate the proliferative status of a target cell population. The kits of the present invention and methods therein described may be used for determining the proliferative status of any isolated cell line or type. The kits and methods of the present invention address the need for rapid assays that determine the proliferative status of isolated hematopoietic stem and progenitor cells and of subpopulations of differentiated cells thereof.
    Type: Grant
    Filed: August 21, 2003
    Date of Patent: April 8, 2008
    Assignee: HemoGenix, Inc.
    Inventor: Ivan N. Rich
  • Patent number: 7198948
    Abstract: We describe an improved method for generating sizable numbers of mature dendritic cells from nonproliferating progenitors in human blood. The first step or “priming” phase is a culture of T cell depleted mononuclear cells in medium supplemented with GM-CSF and IL-4 to produce immature dendritic cells. The second step or “differentiation” phase requires the exposure to dendritic cell maturation factor such as monocyte conditioned medium. Using this two-step approach, substantial yields are obtained. The dendritic cells derive from this method have all the features of mature cells. They include a stellate cell shape, nonadherence to plastic, and very strong T cell stimulatory activity. The mature dendritic cells produced according to this invention are useful for activating T cells.
    Type: Grant
    Filed: January 15, 2002
    Date of Patent: April 3, 2007
    Assignees: The Rockefeller University, Argos Therapeutics, Inc.
    Inventors: Ralph M. Steinman, Nina Bhardwaj, Gerold Schuler
  • Patent number: 7169605
    Abstract: A method of ex-vivo expanding a population of stem cells, while at the same time inhibiting differentiation of the stem cells.
    Type: Grant
    Filed: March 4, 2003
    Date of Patent: January 30, 2007
    Assignees: Gamida Cell Ltd., Hadasit Medical Research Services and Development Ltd.
    Inventors: Tony Peled, Eitan Fibach, Avi Treves
  • Patent number: 7087431
    Abstract: The present invention provides cultured leukemia cells. The method comprises isolating mononuclear cells, which contain leukemia cells, culturing the leukemia cells in a chamber having a scaffolding covered or surrounded with culture medium, where the scaffolding allows for leukemia cells to have cell to cell contacts in three dimensions. The subject leukemia cells are useful for screening compounds which inhibit or stimulate leukemia cell function or formation.
    Type: Grant
    Filed: March 1, 2001
    Date of Patent: August 8, 2006
    Assignee: University of Rochester
    Inventors: J. H. David Wu, Athanassios Mantalaris, Nicki Panoskaltsis
  • Patent number: 7029671
    Abstract: The invention relates to a method for activating human-derived antigen-presenting cells by in vitro cultivation with at least one of the glycoside compounds represented by formula (A) or salts thereof [preferred example: (2S,3S,4R)-1-(?-D-galactopyranosyloxy)-2-hexacosanoylamino-3,4-octadecanediol], and to human antigen-presenting cells activated by the method. The invention also relates to a method for treatment of cancer and infectious diseases including AIDS with the activated human antigen-presenting cells, and to a use of the activated human antigen-presenting cells in the preparation of medicines for treating such diseases. The invention can provide a satisfactory therapeutic effect on cancer and infectious diseases including AIDS without the need to pulse the human antigen-presenting cells with tumor antigens.
    Type: Grant
    Filed: November 27, 2000
    Date of Patent: April 18, 2006
    Assignee: Kirin Brewery Company, Limited
    Inventors: Yasuhiko Koezuka, Yasunori Yamaguchi, Kazuhiro Motoki
  • Patent number: 6982168
    Abstract: The present invention relates to immortalized, malignant, human, adult prostate epithelial cell lines or cell lines derived therefrom useful in the diagnosis and treatment of prostate cancer. More particularly, the present invention relates to cloned, immortalized, malignant, human, adult prostate epithelial cell lines and uses of these cell lines for the diagnosis and treatment of cancer. Furthermore, the present invention provides for the characterization of said cell lines through the analysis of specific chromosomal deletions.
    Type: Grant
    Filed: January 30, 1997
    Date of Patent: January 3, 2006
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: Suzanne L. Topalian, W. Marston Linehan, Robert K. Bright, Cathy D. Vocke
  • Patent number: 6969609
    Abstract: The present invention is a recombinant vector encoding and expressing at least three or more costimulatory molecules. The recombinant vector may additionally contain a gene encoding one or more target antigens or immunological epitope thereof. The synergistic effect of them costimulatory molecules on the enhanced activation of T cells is demonstrated. The degree of T-cell activation using recombinant vectors containing genes encoding three costimulatory molecules was far greater than the sum of recombinant vector constructs containing one costimulatory molecule and greater that the use of two costimulatory molecules. Results employing the triple costimulatory vectors were most dramatic under conditions of either low levels of first signal or low stimulator to T-cell ratios. This phenomenon was observed with both isolated CD4+and CD8+T cells.
    Type: Grant
    Filed: November 12, 1999
    Date of Patent: November 29, 2005
    Assignee: The United States of America as represented by the Department of Health and Human Serivces
    Inventors: Jeffrey Schlom, James Hodge, Dennis Panicali
  • Patent number: 6946293
    Abstract: The present invention relates to a substantially pure population of viable pancreatic progenitor cells, and methods for isolating such cells. The present invention further concerns certain therapeutic uses for such progenitor cells, and their progeny.
    Type: Grant
    Filed: August 9, 2000
    Date of Patent: September 20, 2005
    Inventors: Kuanghui Lu, Kevin Pang, Lee Rubin
  • Patent number: 6919206
    Abstract: Ligands for flt3 receptors capable of transducing self-renewal signals to regulate the growth, proliferation or differentiation of progenitor cells and stem cells are disclosed. The invention is directed to flt3-L as an isolated protein, the DNA encoding the flt3-L, host cells transfected with cDNAs encoding flt3-L, compositions comprising flt3-L, methods of improving gene transfer to a mammal using flt3-L, and methods of improving transplantations using flt3-L. Flt3 -L finds use in treating patients with anemia, AIDS and various cancers.
    Type: Grant
    Filed: December 19, 1997
    Date of Patent: July 19, 2005
    Assignee: Immunex Corporation
    Inventors: Stewart D. Lyman, M. Patricia Beckmann