Abstract: A liquid ejection unit includes: a liquid holding section which has an ejection port through which a liquid is ejected and which holds the liquid; a pressure adjustment section which is configured to adjust a pressure of a liquid held in the liquid holding section; and a displacement member which is configured to displace at least a part of the liquid whose pressure is adjusted and eject the liquid from the liquid holding section.

Abstract: A microfluidic structure in which a plurality of chambers arranged at different positions are connected in parallel and into which a fixed amount of fluid may be efficiently distributed without using a separate driving source, and a microfluidic device having the same. The microfluidic device includes a platform having a center of rotation and including at least one microfluidic structure. The microfluidic structure includes a sample supply chamber configured to accommodate a sample, a plurality of first chambers arranged in a circumferential direction of the platform at different distances from the center of rotation of the platform, and a plurality of siphon channels, each of the siphon channels being connected to a corresponding one of the first chambers.

Abstract: It is necessary to efficiently agitate a small amount of sample (such as blood) and reagent (such as diluted solution) in a short time with a dispensing probe having a constant tube inner diameter. An automatic analysis device, by a dispensing probe, executes an aspiration step of aspirating a reagent from a reagent vessel; a first dispensing and aspiration step of dispensing a first liquid amount of the aspirated reagent to a reaction vessel and aspirating, from the reaction vessel, a second liquid amount of a mixed liquid obtained by mixing the reagent and the sample in the reaction vessel; and a final dispensing step of dispensing the aspirated mixed liquid and the reagent in a predetermined dispensing amount. A first liquid amount Va is less than a predetermined dispensing amount Vdil.

Abstract: A microfluidic substrate and a manufacture method thereof, and a microfluidic panel are provided. The microfluidic substrate includes a base substrate, an acoustic wave generation device, and a first switching circuit. The acoustic wave generation device is on the base substrate and configured to emit an acoustic wave to drive a liquid droplet to move over the microfluidic substrates, the acoustic wave generation devices includes an acoustic wave driving electrode and an acoustic wave generation layer, the first switching circuit is on the base substrate, and the first switching circuit is electrically connected to the acoustic wave driving electrode and is configured to transmit an acoustic wave driving signal to the acoustic wave driving electrode, and the acoustic wave driving electrode is configured to drive the acoustic wave generation layer to generate the acoustic wave under control of the acoustic wave driving signal.

Abstract: Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.

Abstract: A method for filling an analytical device includes providing a plate having a detection space or spaces and ports communicating with the space(s), fitting, into one of the ports, an adapter having a reagent fixed on an inner wall of the adapter such that a first end of the adapter is fitted into the port, and delivering a sample solution into the detection space(s) through the adapter such that the sample solution and reagent fill the detection space(s). The plate includes a first substrate, a second substrate, and a side wall such that the plate has the detection space(s) between the substrates and that the first substrate has the ports, the first and/or second substrates is formed of transparent material such that the detection space is observable from outside the plate, and the ports include a first port for delivering liquid-containing substance, and a second port for discharging the substance.

Abstract: The present disclosure provides a microfluidic chip and a droplet separation method, and belongs to the field of biological chip technology. The microfluidic chip includes a first liquid tank and a second liquid tank opposite to each other and a channel layer therebetween. The channel layer includes a plurality of microfluidic channels separated from each other, first ends of the microfluidic channels are communicated with the first liquid tank, and second ends are communicated with the second liquid tank. The first liquid tank contains sample solution to be detected, and the second liquid tank contains encapsulating liquid. The sample solution to be detected entering the first liquid tank may be separated into a plurality of sample droplets through the microfluidic channels, the separated sample droplets enter the second liquid tank, so that the encapsulating liquid is encapsulated on a surface of each of the plurality of sample droplets.

Abstract: Diagnostic detection chip device designs that reduce cost of fabrication and assembly are described herein. Such chip device designs include features that facilitate use of the chip within a chip carrier device with integrated fluid flow control features and compatibility with conventional sample cartridges and sample processing systems. Associated methods of manufacture and assembly of the chip devices are also provided herein.

Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

Abstract: Devices, methods, and kits for detecting at least two analytes present within a small volume single fluid sample obtained from patient for the creation of a multiplexed panel of the various analytes present within the single fluid sample are disclosed.

Abstract: A microfluidic device comprises upper and lower spaced apart substrates defining a fluid chamber therebetween; an aperture for introducing fluid into the fluid chamber; and a fluid input structure disposed over the upper substrate and having a fluid well for receiving fluid from a fluid applicator inserted into the fluid well. The fluid well communicates with a fluid exit provided in a base of the fluid input structure, the fluid exit being adjacent the aperture. The fluid well comprises first, second and third portions, with the first portion of the well forming a reservoir for a filler fluid; and the second portion of the well being configured to sealingly engage against an outer surface of a fluid applicator inserted into the fluid well. The third portion of the well communicates with the fluid exit and has a diameter at the interface between the third portion and the second portion that is greater than the diameter of the second portion at the interface between the third portion and the second portion.

Abstract: A method of encapsulating a solid sample in a droplet, the method including flowing a continuous phase through a first fluid channel at a first flow rate; flowing a dispersed phase through a second fluid channel at a second flow rate, the dispersed phase including a plurality of particles, cells or beads; trapping the plurality of particles, cells or beads in a mixing region that receives the dispersed phase and the continuous phase; and reducing the first flow rate to encapsulate the trapped particles, cells or beads in droplets of the dispersed phase generated when the dispersed phase and the continuous phase exit the mixing region through an orifice.

Abstract: Described is a mixer for a chromatography system. The mixer includes an inlet manifold channel, an outlet manifold channel and a plurality of transfer channels. The inlet manifold channel has an inlet at a proximal end of the inlet manifold channel for receiving an inlet flow. The transfer channels are fluidly connected between the inlet and outlet manifold channels. The respective fluid connections are distributed along each of the inlet and outlet manifolds channels. The transfer channels have different volumes. The mixer may be formed of a plurality of layer and the layers may be diffusion bonded to each other.

Abstract: Described herein are sample loading systems for loading a sample into a processing and/or analysis system comprising: a sample reservoir for receiving a sample and a metering volume reservoir, the sample reservoir and a first side of the metering volume reservoir being interconnected through a first channel with a first flow resistance to allow filling of the metering volume reservoir with sample; a further reservoir for receiving a second fluid interconnected with the metering volume reservoir at the first side via a second channel having a smaller second flow resistance; a first valve for blocking flow of sample from the metering volume reservoir into the second channel; a second valve connected to a second side of the metering volume reservoir for controlling the blocking and flowing of sample; and a first timing circuitry for timing the opening of the second valve as a function of filling of the further reservoir.

Abstract: Embodiments of the invention provide a microfluidic chip having microfluidic structures formed on a surface. The structures form an input channel, an output channel, auxiliary channels, and a hydraulic resistor structure connecting the input channel to the output channel via the auxiliary channels. The resistor structure includes N flow resistor portions (N?2), which are connected to the auxiliary channels. The chip further includes at least N?1 actuatable valves, which are arranged in respective ones of the auxiliary channels. The actuation state of the valves can determine the effective hydraulic resistance of the resistor structure. The valves can be electrogates, each including a liquid-pinning trench arranged in a respective one of the auxiliary channels that define a flow path for a liquid introduced therein, so as to form an opening that extends across said flow path. Each electrogate can further include an electrode extending across the flow path.

Abstract: Aspects of the present disclosure relate to methods and devices for automatically transferring samples and/or reagents from sample vessels and/or reagent vessels into at least one receiving vessel In one example embodiment, a pipetting device is disclosed including a pipettor that is movable along a first direction and has at least one first pipetting needle that is movable along an arm of the pipettor along a second direction, substantially normal to the first direction. The pipetting needle is lowerable along a third direction into the individual vessels. In some specific embodiments, the arm of the movable pipettor has at least one second pipetting needle which, regardless of the current position of the first pipetting needle, is movable past the first pipetting needle and is lowerable into the individual vessels.

Abstract: Fluid storage containers and analysis cartridges for use in assay processes are presented. In addition, systems comprising such storage containers and analysis cartridges and methods of using such containers and cartridges are presented as well. In specific embodiments, fluid storage containers are configured to be coupled to analysis cartridges in a first stage and a second stage.

Abstract: A perforate element for use in a print head for non-contact liquid printing comprises: at least one ejection element including an outlet, configured to eject a bulk flow of printing liquid out of the print head; and a liquid residence element, arranged to provide a layer of liquid over the outlet which extends laterally of the outlet and through which the bulk flow is ejected.

Abstract: An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.

Abstract: Aspects herein relate to coating apparatus and methods for coating medical devices. In an embodiment, a coating system is included having a valve, a fluid supply reservoir in fluid communication with the valve, a reciprocating positive displacement pump in fluid communication with the valve, and a fluid applicator in fluid communication with the three-way valve. The valve can be configured to assume a first fluid transport state and a second fluid transport state, wherein the valve provides fluid communication between the fluid supply reservoir and the reciprocating positive displacement pump when in the first fluid transport state for filling of the reciprocating positive displacement pump, and wherein the valve provides fluid communication between the reciprocating positive displacement pump and the fluid applicator when in the second fluid transport state for applying a coating suspension to a medical device surface. Other embodiments are also included herein.

Abstract: A fluid chip suitable for a fluid device is disclosed in which an upper surface of a flow passage has another member bonded thereto. The disclosed fluid chip, in which a flow passage is formed, comprises a base material having a top surface forming at least a portion of a bottom surface of a flow passage, and a bonding member which is formed from an elastomer resin and an upper end surface of which is provided in a position higher than the top surface of the base material. The base material has a support post portion which projects from the top surface and defines the height of a side surface of the flow passage, and the support post portion of the base material is embedded in the bonding member.

Abstract: Certain embodiments are directed to finite step emulsification device and/or methods that combine finite step emulsification with gradients of confinement for the formation of a 2D monolayer array of droplets with low size dispersion.

Abstract: A carrier for consumables which are mainly used in laboratory (analytic) applications and provides a carrier for consumables, comprising a ground plate with at least two cut-outs for accommodating a consumable, a clamp plate with at least one clamp extending partially above the cut-outs and a locking lever movably connected with the clamp plate, wherein the locking lever has at least a cam located at an end connected with the clamp plate, wherein the cam presses in a locking position of the locking lever the clamp plate towards the ground plate and releases in a releasing position a gap between ground plate and clamp plate.

Abstract: A digital slide scanning apparatus that includes a scanning stage, a focusing sensor, an imaging sensor, and at least two processors. A main processor is configured to control the scanning stage to move a sample relative to the focusing sensor and the imaging sensor. The main processor controls the secondary processor to process focus buffers generated by the focusing sensor and image buffers generated by the imaging sensor. The secondary processor access each buffer and processes the data in the buffer to generate an average contrast vector for the buffer. The average contrast vectors for the focus and image buffers are then provided to the main processor for further processing in connection with autofocus and/or generation of a digital slide image.

Abstract: A method is disclosed for controlling at least one of aspirating and of dispensing a liquid dose or of producing a liquid dose in a pipette or in receptacle, the method having the steps of first loading a first pipette with a first working medium at a first pressure having a first sign with respect to a reference pressure, thereby dispensing the liquid dose into the receptacle or aspirating the liquid dose into the first pipette, second loading a second pipette with a second working medium at a second pressure having a second sign with respect to the reference pressure, thereby dispensing the liquid dose into the receptacle or aspirating the liquid dose into the second pipette, discharging a pressure in the first working medium through a controlled valve arrangement to the reference pressure between first and second loadings. A pipetting device is also disclosed.

Abstract: A vacuum manifold for filtration microscopy includes a manifold top having multiple openings, and a capture membrane positioned above and spaced apart from the manifold top, where the capture membrane is configured to deflect into contact with a surface of the manifold top when a negative pressure is applied to the multiple openings. A method for filtration microscopy includes the steps of providing a vacuum manifold including a manifold top having a plurality of openings, and a capture membrane positioned above and spaced apart from the manifold top; applying sample drops to sample spots on the membrane, the sample spots positioned above the plurality of openings; applying a negative pressure to the openings such that the capture membrane contacts a surface of the manifold top; and optically imaging particulates on the capture membrane.

Abstract: A testing assembly for the rapid detection of a desired chemical from a sample, the testing assembly having a sample collection device for obtaining the sample and depositing the sample into a chemical capture cartridge that operably engages with a sensing device, the chemical capture cartridge utilizing the microdiffusion of reagents to isolate the desired chemical from the sample and react the desired chemical to form a detectible complex that can be detected and measured. The testing assembly can be portable and can rapidly diagnose a toxic industrial chemical exposure in just a few minutes, preferably about a minute or less for cyanide exposure using a blood sample from a subject onsite. The testing assembly can be used to determine the presence and/or concentration of a toxic industrial chemical in a variety of samples, including biological, environmental, and industrial samples.

Abstract: Provided is a fluid treatment system, including: a plurality of fluid channel devices arranged in series along a regular channel; a plurality of flow control valves each adjusting the flow rate of a treatment target fluid flowing into each of the plurality of fluid channel devices; a flow control valve provided on the upstream side of the plurality of fluid channel device and operable to change the flow rate of the treatment target fluid flowing into each of the plurality of fluid channel device; a bypass channel allowing the treatment target fluid to flow so as to bypass the fluid channel device in which abnormality has occurred, and a plurality of bypass selector valves selectable between a state of allowing the flow of the treatment target fluid in the bypass channel and a state of blocking the flow.

Abstract: A system that include an sample analyzer having a sensor array. The sensor array includes a housing having a base, a top spaced above the base, and an outer wall that extends from the base to the top. The sensor array includes an inlet that is sized to receive a sample of the fluid, and a plurality of partitions arranged around the fluid inlet. Each partition has a port at the fluid inlet for receiving a portion of the sample of fluid received by the fluid inlet. The sensor array includes at least one sensor in each partition. The sensor array is configured to selectively direct the sample of fluid received by the fluid inlet into one or more of the plurality of partitions into contact the at least one sensor.

Abstract: A method and system are provided for extracting a target analyte from a sample using acoustic ejection technology. The method involves applying focused acoustic energy to a fluid reservoir housing a fluid composition that contains a target analyte and comprises an upper region and a lower region, where the concentration of the target analyte in the upper region differs from that in the lower region. The focused acoustic energy is applied in a manner that is effective to result in the ejection of a fluid droplet from the fluid composition into a droplet receiver, wherein the concentration of the analyte in the droplet corresponds to either the concentration of the analyte in the upper region or the concentration of the analyte in the lower region, and wherein the concentration of the analyte is substantially uniform throughout the droplet. The fluid composition may comprise an ionic liquid, used in the extraction of ionic target analytes. Related methods and an acoustic extraction system are also provided.

Abstract: A test system for analyzing a sample of a bodily fluid is provided and comprises: at least one test strip comprising at least one capillary channel comprising an inlet opening configured to receive the sample; a vent opening configured to provide an air vent to the capillary channel; and at least one zone consisting of a detection zone and a reagent zone; at least one measuring device configured for interacting with the test strip, the measuring device comprising at least one sealing element for hermetically sealing the vent opening from an ambient atmosphere; and at least one suction device adapted to provide an underpressure to the vent opening; wherein the measuring device further comprises at least one valve or is connectable to the valve, wherein the valve is configured to vent the vent opening of the test strip when the measuring device interacts with the test strip.

Abstract: Examples include a fluid device. The fluid device includes a substrate, a sensor coupled on the substrate. A reservoir is formed in the substrate adjacent to the sensor. A deformable cover is disposed to seal the sensor and the reservoir on the substrate.

Abstract: Provided are methods for the automated loading and/or automatic processing of one or more samples in an automated sample processing device. Also provided are automated sample loading systems and devices that include automated sample loading systems or devices that are utilized in such systems.

Abstract: A liquid discharge head includes a nozzle to discharge a liquid, an individual chamber communicating with the nozzle, a supply channel communicating with the individual chamber to supply the liquid to the individual chamber, and a discharge channel communicating with the individual chamber to discharge the liquid in the individual chamber. A fluid resistance of the supply channel is greater than a fluid resistance of the discharge channel.

Abstract: There is provided a cartridge for nucleic acid extraction comprising: a first body having a plurality of chambers in which ports are formed at the bottom; a second body coupled to a lower region of the first body; and a piston disposed rotatably in the centers of the first body and the second body and having a port formed at the bottom thereof; and characterized in that the cartridge comprises a plurality of flow paths formed on the upper region of the second body, one end overlapping the port of the piston and the other end overlapping the port of the first body.

Abstract: Disclosed herein are membrane device embodiments that can be used for separating blood plasma and/or blood serum from blood samples. The membrane device embodiments comprise built-in features that facilitate blood plasma and/or blood serum separation and also provide the ability to detect, quantify, and qualify analytes present in a blood sample. The membrane device embodiments are portable and just a single membrane can be used for a plethora of detection and analysis techniques. Also disclosed herein are embodiments of methods for making and using the membrane device.

Abstract: According to an example, a microfluidic apparatus may include a channel, a foyer, in which the foyer is in fluid communication with the channel and in which the channel has a smaller width than the foyer, a sensor to sense a property of a fluid passing through the channel, a nozzle in fluid communication with the foyer, and an actuator positioned in line with the nozzle. The microfluidic apparatus may also include a controller to determine whether the sensed property of the fluid meets a predetermined condition and to perform a predefined action in response to the sensed property of the fluid meeting the predetermined condition.

Abstract: A fluidic device includes a first circulation flow path and a second circulation flow path which circulate a solution containing a sample material, the first circulation flow path and the second circulation flow path share at least a part of the flow path, and at least one selected from the group consisting of a capture unit which captures the sample material, a detection unit which detects the sample material, a valve, and a pump is provided on the shared flow path.

Abstract: Different Au—Pd nanoparticles, ranging from sharp-branched octopods to core@shell octahedra, can be achieved by inline manipulation of reagent flowrates in a microreactor for seeded growth. Significantly, these structures represent different kinetic products, demonstrating an inline control strategy toward kinetic nanoparticle products that should be generally applicable.

Abstract: The devices, methods, and compositions disclosed herein accomplish robust cell encapsulation in polymer microparticles using a vertically oriented microfluidic device. A hydrophilic polymer precursor solution is flowed into a first inlet channel, which extends inward from an upper surface of the device housing. A hydrophobic fluid is flowed into a second inlet channel, which extends inward from a lower surface of the device housing. The two inlet channels meet at a junction, and an outlet channel extends away from the two inlet channels. When the two inwardly flowing streams meet at the junction, the polymer precursor solution disperses into the hydrophobic fluid. The dispersed precursor droplets are photopolymerized into microparticles as they travel through the outlet channel. The resulting microparticles are highly uniform, and are larger than conventionally formed microparticles. Cells of varying types can be encapsulated with high viability and spatial uniformity.

Abstract: Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.

Abstract: The disclosure provides a microfluidic detection chip, a preparation method thereof, a fixing device and a centrifugal detection device. The detection chip comprises overlapped three layers. The upper layer of the chip includes a sample loading area and vents; the lower layer of the chip includes a waste liquid tank area in which a slope structure or a groove is disposed; the intermediate layer of the chip is a double-sided adhesive layer on which sample flow channels are divided by an adhesive area and an adhesive-free area. The self-driving and short-time centrifugation combined microfluidic detection chip technology designed by the present disclosure can solve inherent problems of traditional paper substrates, further improve sample utilization, detection speed and detection sensitivity in immunoassay, and the preparation and assembly of the chip are simple, has low requirement for the detection device, and can be conveniently applied to the clinical detection.

Abstract: A method of partitioning droplets from a fluid reservoir containing particles provides a non-Poissonian distribution of dispensed droplets containing a desired number of particles.

Abstract: A method of additive manufacturing includes supplying additive manufacturing powder to a build area of an additive manufacturing machine. The method includes fusing a portion of the powder to form a part, and removing a non-fused portion of the powder from the build area into a removable vessel for storing non-fused powder after building a part. The method can include supplying additive manufacturing powder to a build area, fusing a portion of the powder, and removing a non-fused portion of the powder all on a single discrete lot of additive manufacturing powder without mixing lots.

Abstract: An array of membranes comprising amphipathic molecules is formed using an apparatus comprising a support defining an array of compartments. Volumes comprising polar medium are provided within respective compartments and a layer comprising apolar medium is provided extending across the openings with the volumes. Polar medium is flowed across the support to displace apolar medium and form a layer in contact with the volumes, forming membranes comprising amphipathic molecules at the interfaces. In one construction of the apparatus, the support that comprises partitions which comprise inner portions and outer portions. The inner portions define inner recesses without gaps therebetween that are capable of constraining the volumes comprising polar medium contained in neighbouring inner recesses from contacting each other. The outer portions extend outwardly from the inner portions and have gaps allowing the flow of an apolar medium across the substrate.

Abstract: The invention relates to a method for flexibly controlling the use of hydrochloric acid having an HCl concentration of at least 10 wt %, in particular at a volume flow rate of at least 1 m3/h, obtained from a continuous chemical production process (A). In the method, purified hydrochloric acid (54) from a hydrochloric acid store (E) is optionally fed to a dispatch station (H), an HCl electrolysis station (F) and a chloralkali electrolysis station (L), which are consumption points for the hydrochloric acid, or to a neutralisation station (G) in that if one or more of said consumption points (H, F, L) is not available or if there are bottlenecks at the consumption points (H, F, L), the hydrochloric acid (54) is fed to the neutralisation station (G) and neutralised with concentrated alkali solution (55), in particular with concentrated sodium hydroxide solution, and the resulting salt solution (56) is fed either to the chloralkali process station (L) or to a disposal station (M).

Abstract: Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.

Abstract: Methods and apparatus for controlling flow in a microfluidic arrangement are disclosed. In one arrangement, a microfluidic arrangement comprises a first liquid held predominantly by surface tension in a shape defining a microfluidic pattern on a surface of a substrate. The microfluidic pattern comprises at least an elongate conduit and a first reservoir. A second liquid is in direct contact with the first liquid and covers the microfluidic pattern. A flow of liquid is driven through the elongate conduit into the first reservoir.

Abstract: A microfluidic AM-EWOD device and a method of filling such a device are provided. The device comprises a chamber having one or more inlet ports. The device is configured, when the chamber contains a metered volume of a filler fluid that partially fills the chamber, preferentially maintain the metered volume of the filler fluid in a part of the chamber. The device is configured to allow displacement of some of the filler fluid from the part of the chamber when a volume of an assay fluid introduced into one of the one or more inlet ports enters the part of the chamber, thereby causing a volume of a venting fluid to vent from the chamber.

Abstract: Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.