Patents Represented by Attorney, Agent or Law Firm Barbara Rae-Venter
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Patent number: 5792467Abstract: Repellent compositions which contain aromatic aldehydes such as cinnamic aldehyde, .alpha.-hexyl cinnamic aldehyde and/or coniferyl aldehyde are provided, together with methods for their use as repellents for pests including flies, cockroaches, aphids, silverleaf white flies, mosquitoes, ticks, fleas, lice, leafhoppers, thrips, two-spotted spider mites, snails, slugs, biting midges, earwigs, and moths. Also provided are stabilized .alpha.-hexylcinnamic aldehyde (HCA) compositions which reduce the evaporation rate of HCA and/or induce HCA to evaporate at a constant rate over time.Type: GrantFiled: December 24, 1996Date of Patent: August 11, 1998Assignee: Proguard, Inc.Inventors: Ralph W. Emerson, Bradford G. Crandall, Jr.
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Patent number: 5738861Abstract: The present invention provides methods and compositions for inhibiting microbial growth through the use of flavonoid aldehydes or alcohols for the purpose of disinfecting contaminated environments. The methods include the step of contacting the unsterile area with an amount of a flavonoid aldehyde or alcohol sufficient to control growth of pathogenic microbes. The aldehyde or alcohol can be provided in a variety of formulations.Type: GrantFiled: November 22, 1995Date of Patent: April 14, 1998Assignee: Proguard, Inc.Inventors: Ralph W. Emerson, Bradford G. Crandall, Jr.
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Patent number: 5643791Abstract: A biologically pure DNA sequence which encodes an endo 1,4-.beta.-glucanase, useful for the efficient conversion of cellulose to glucose, is disclosed. Also disclosed are recombinant DNA cloning vehicles (vectors) that contain nucleotide sequences that encode for the aforesaid endoglucanase, proteins that exhibit endoglucanase activity, expression-controlling DNA sequences, microorganisms that contain recombinant DNA cloning vehicles, as well as messenger RNA sequences complementary to a DNA strand of the aforesaid DNA nucleotide sequences.Type: GrantFiled: January 19, 1994Date of Patent: July 1, 1997Assignee: University of British ColumbiaInventors: R. Anthony J. Warren, Douglas G. Kilburn, Robert C. Miller, Jr.
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Patent number: 5629149Abstract: The present invention provides a process for determining genotypes in highly polymorphic systems by polymerase chain reaction amplification of cDNA or genomic DNA and direct sequencing polymerase chain reaction products using oligonucleotide primers. More specifically, Class II and Class I HLA genotypes can be unambiguously determined in any subject in 16-24 hours by direct sequencing of DRB, DQB, DQA, DPB, DPA, HLA-A, HLA-B and HLA-C- transcripts enzymatically amplified using a limited number of non-allele-specific oligonucleotides. Total cellular RNA from peripheral blood mononuclear cells is reverse transcribed using antisense primers, specific for different locus (DQB, DQA, DPA or DPB) or group of loci (DRB1-5, or HLA-A and HLA-B and HLA-C). The synthesized cDNA molecules are then enzymatically amplified using different combinations of oligonucleotides for each locus and directly sequenced with Taq polymerase using an internal oligonucleotide. The sequenced genes are then analyzed.Type: GrantFiled: August 16, 1993Date of Patent: May 13, 1997Assignee: Regents of the University of MinnesotaInventors: Pedro Santamaria, Michael T. Boyce-Jacino, Jose J. Barbosa, Stephen S. Rich, Anthony J. Faras
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Patent number: 5538878Abstract: Plant species having enhanced superoxide dismutase activity as a result of transformation with a DNA expression cassette comprising an E. coli MnSOD gene are provided. Transportation of the expression product of the gene may be targeted to a specific cell organelle, such as the chloroplast.Type: GrantFiled: July 19, 1989Date of Patent: July 23, 1996Assignees: Calgene, Inc., ProcidaInventors: Bruce Thomas, H. Maelor Davies, Jean Kridl, Jeffery K. O'Neal, C. Jacques Van Assche
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Patent number: 5530185Abstract: Novel DNA constructs are provided which may be used as molecular probes or inserted into a plant host to provide for modification of transcription of a DNA sequence of interest in ovary tissue, particularly in very early fruit development. The DNA constructs comprise a transcriptional initiation regulatory region associated with gene expression in ovary tissue from immediately prior to anthesis through flower senescence.Type: GrantFiled: December 29, 1992Date of Patent: June 25, 1996Assignee: Calgene, Inc.Inventors: Belinda M. Martineau, David M. Stalker, Ann A. Reilley
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Patent number: 5529917Abstract: Novel microbial host strains are provided which are transformed by a vector molecule comprising a DNA fragment encoding a lipolytic enzyme and a marker for selection, capable of producing active lipase. Said DNA fragment is preferably derived from a Pseudomonas species.Type: GrantFiled: January 11, 1994Date of Patent: June 25, 1996Assignee: Gist-brocadesInventors: Peter M. Andreoli, Maria M. J. Cox, Farrokh Farin, Suzanne Wohlfarth-Rippel
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Patent number: 5513661Abstract: Methods are provided for intramuscular needle diagnosis and treatment of muscle pain that is believed to be the result of sympathetically mediated spindle spasm. Two simultaneous needle EMG needle recordings are used to establish the presence of and magnitude of the "trigger points" in painful muscle. The abnormal muscle activity so identified can then be treated by blocking the sympathetic activation muscle blocker or other agent that would have the same effect of inhibiting the abnormal muscle activity.Type: GrantFiled: October 14, 1993Date of Patent: May 7, 1996Inventor: David R. Hubbard
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Patent number: 5462862Abstract: Clustered antibiotic biosynthetic genes are employed for improvement of production of the antibiotic in microorganisms and for the isolation of other genes involved in the biosynthesis of the antibiotic. The invention is exemplified with improved production of penicillin in Penicillium chrysogenum, with the isolation of another clustered biosynthetic gene(s) and with the expression of penicillin biosynthetic genes in Acremonium chrysogenum.Type: GrantFiled: January 25, 1993Date of Patent: October 31, 1995Assignee: Gist-brocades N.V.Inventors: Martinus A. M. Groenen, Annemarie E. Veenstra, Pieter Van Solingen, Bertus P. Koekman, Lucia H. M. Van Der Voort, Juan F. Martin, Santiago Gutierrez, Bruno Diez, Emilio Alvarez, Jose L. Barredo, Christina Esmahan
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Patent number: 5457032Abstract: New mutant .beta.-lactam acylases are provided exhibiting altered substrate specificities. These .beta.-lactam acylases are obtained by expression of a gene encoding said .beta.-lactam acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type .beta.-lactam acylase.Type: GrantFiled: May 9, 1991Date of Patent: October 10, 1995Assignee: Gist-brocades NVInventors: Wilhelmus J. Quax, Onno Misset, Jan M. Van Der Laan, Herman B. M. Lenting
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Patent number: 5453566Abstract: Regulation of expression of genes encoded for in plant cell genomes is achieved by integration of a gene under the transcriptional control of a promoter which is functional in the host and in which the transcribed strand of DNA is complementary to the strand of DNA that is transcribed from the endogenous gene(s) one wishes to regulate. The integrated gene, referred to as antisense, provides an RNA sequence capable of binding to naturally existing RNAs, exemplified by polygalacturonase, and inhibiting their expression, where the anti-sense sequence may bind to the coding, non-coding, or both, portions of the RNA. The antisense construction may be introduced into the plant cells in a variety of ways and be integrated into the plant genome for inducible or constitutive transcription of the antisense sequence. A wide variety of plant cell properties may be modified by employing this technique.Type: GrantFiled: August 27, 1991Date of Patent: September 26, 1995Assignee: Calgene, Inc.Inventors: Christine K. Shewmaker, Jean C. Kridl, William R. Hiatt, Vic Knauf
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Patent number: 5441937Abstract: Novel neurotrophic factor compositions are provided, obtained from lung tissue. The factors are found to be active on parasympathetic ganglion neurons enhancing acetylcholine activity. The compositions find use in the treatment of a number of eye disorders.Type: GrantFiled: January 21, 1994Date of Patent: August 15, 1995Assignee: Houston Biotechnology IncorporatedInventors: Thomas L. Wallace, David E. Potter, Craig E. Crosson
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Patent number: 5424184Abstract: The present invention provides a process for determining genotypes in highly polymorphic systems by polymerase chain reaction amplification of cDNA or genomic DNA and direct sequencing polymerase chain reaction products using oligonucleotide primers. More specifically, Class I HLA genotypes can be unambiguously determined in any subject in 16-24 hours by direct sequencing of HLA-A, HLA-B, and HLA-C transcripts enzymatically amplified and sequenced using a limited number of selected oligonucleotides.Type: GrantFiled: May 8, 1991Date of Patent: June 13, 1995Assignee: Regents of the University of MinnesotaInventors: Pedro Santamaria, Michael T. Boyce-Jacino, Jose Barbosa, Stephen S. Rich, Anthony J. Faras
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Patent number: 5420034Abstract: Nucleic acid sequences and methods for their use are provided which provide for seed-specific transcription, in order to modulate or modify expression in seed, particularly embryo cells. Transcriptional initiation regions are identified and isolated from plant cells such as seed embryo and seed coat and used to prepare expression cassettes which may then be transformed into plant cells for seed-specific transcription. The method finds particular use in conjunction with modifying fatty acid production in seed tissue.Type: GrantFiled: August 8, 1991Date of Patent: May 30, 1995Assignee: Calgene, Inc.Inventors: Jean C. Kridl, Vic C. Knauf
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Patent number: 5364782Abstract: Thermostable and acid stable .alpha.-amylases are provided as expression products of genetically engineered .alpha.-amylase genes isolated from microorganisms, preferably belonging to the class of Bacilli. Both chemical and enzymatic mutagenesis methods are e.g. the bisulphite method and enzymatic misincorporation on gapped heteroduplex DNA. The mutant .alpha.-amylases have superior properties, e.g. improved thermostability over a broad pH range, for industrial application in starch processing and textile desizing.Type: GrantFiled: November 29, 1990Date of Patent: November 15, 1994Assignees: Gist-Brocades N.V., Plant Genetic Systems N.V.Inventors: Wilhelmus J. Quax, Yves Laroche, Adrianus W. H. Vollebregt, Patrick Stanssens, Marc Lauwereys
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Patent number: 5364531Abstract: Immunoaffinity purifications are performed using non-aqueous media, desirably, a small amount of an aqueous buffered medium, and, optionally, a surfactant. Hydrophobic ligands can be extracted from samples, immunoaffinity purified and eluted to provide a substantially pure product.Type: GrantFiled: November 10, 1992Date of Patent: November 15, 1994Assignee: Hawaii Biotechnology Group, Inc.Inventors: Gary S. Bignami, Paul G. Grothaus
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Patent number: 5340731Abstract: A fusion protein that can function as a removable label is prepared containing a polypetide such as an enzyme and an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that has essentially no polysaccharidase activity. By contacting the fusion protein with a .beta.-1,4 glycan matrix such as cellulose, the substrate binding region binds to the matrix to immobilize the polypeptide. The polypetide or fusion protein can be removed from the matrix with a protease capable of cleaving a specific protease cleavage site, or with a solution having a low ionic strength or a high pH. The fusion protein can be prepared by recombinant DNA technology.Type: GrantFiled: April 8, 1992Date of Patent: August 23, 1994Assignee: University of British ColumbiaInventors: Douglas G. Kilburn, Robert C. Miller, Neil Gilkes, R. Antony J. Warren
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Patent number: 5336611Abstract: New proteolytic enzymes are provided exhibiting improved properties for application in detergents, especially laundry detergents. These enzymes are obtained by expression of a gene encoding a proteolytic enzyme having an amino acid sequence which differs at least in one amino acid from the wild type enzyme. Preferred enzymes are certain mutants derived from the serine protease of Bacillus nov. spec. PB92.Type: GrantFiled: October 11, 1989Date of Patent: August 9, 1994Assignee: Gist-brocades N.V.Inventors: Christiaan A. G. van Eekelen, Leonardus J. S. M. Mulleners, Johannes C. Van Der Laan, Onno Misset, Roelck A. Cuperus, Johan H. A. Lensink
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Patent number: 5328839Abstract: A new oxido reductase enzyme activity obtainable from P. chrysogenum, involved in the production of .beta.-lactams, the set of genes encoding said enzyme activity, and a method to enhance said production by using the above-mentioned oxido reductase system and other oxido reductase systems or genes encoding for the same have been disclosed.Type: GrantFiled: March 24, 1992Date of Patent: July 12, 1994Assignee: Gist-BrocadesInventors: Yair Aharonowitz, Lucia H. M. van der Voort, Gerald Cohen, Roelof A. L. Bovenberg, Rachel Schreiber, Anat Argaman, Yossef Av-Gay, Helena M. Nan, Alfred Kattevilder, Harriet Palissa, Henk van Liempt
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Patent number: 5324653Abstract: New proteolytic enzymes are provided exhibiting improved properties for application in detergents, especially laundry detergents. These enzymes are obtained by expression of a gene encoding a proteolytic enzyme having an amino acid sequence which differs at least in one amino acid from the wild type enzyme. Preferred enzymes are certain mutants derived from the serine protease of Bacillus nov. spec. PB92.Type: GrantFiled: April 24, 1991Date of Patent: June 28, 1994Assignee: Gist-Brocades N.V.Inventors: Christiaan A. G. Van Eekelen, Leonardus J. S. M. Mulleners, Johannes C. Van Der Laan, Onno Misset, Roelck A. Cuperus, Johan H. A. Lensink