Patents Represented by Attorney, Agent or Law Firm Donna R. Fugit
  • Patent number: 6379888
    Abstract: Signal primers are employed for detection of nucleic acid target sequences by fluorescence quenching mechanisms. The signal primer comprises a first and a second oligonucleotide and is partially single-stranded and partially double-stranded. In the presence of target, the second oligonucleotide of the signal primer is displaced from the first and a conformational change in a reporter probe occurs which changes the distance between the members of a donor/quencher dye pair linked to the reporter probe. The change in proximity between the dyes causes an increase or a decrease in fluorescence quenching, which is detected as an indication of the presence of the target sequence.
    Type: Grant
    Filed: September 27, 1999
    Date of Patent: April 30, 2002
    Assignee: Becton, Dickinson and Company
    Inventors: James G. Nadeau, C. Preston Linn, J. Bruce Pitner, Cheryl H. Dean, G. Terrance Walker
  • Patent number: 6380858
    Abstract: Systems and methods are provided for facilitating effective self-management of medication treatment by patients. A Smart Tray monitors and reports to third parties a patient's compliance with various medication treatment regimens. Medication containers are provided with electromagnetic tags that provide various information about medicament contained within a respective container. A Smart Tray is equipped with a processor and reader that interrogates each respective electromagnetic tag to identify medicament(s) contained within each container. Using the retrieved information, a Smart Tray provides visual and/or audio signals to a patient to remind the patient when and how much of various medicaments to take. A Smart Tray also monitors, via the reader, when a medication container is removed. A Smart Tray can communicate with one or more third parties, such as healthcare providers, pharmacies, and other suppliers of healthcare products and services via a computer network.
    Type: Grant
    Filed: August 15, 2001
    Date of Patent: April 30, 2002
    Assignee: Becton, Dickinson and Company
    Inventors: Paul Yarin, Richard Fletcher, Joseph DiPisa, Glenn Philander Vonk
  • Patent number: 6294999
    Abstract: Systems and methods are provided for facilitating effective self-management of medication treatment by patients. A Smart Tray monitors and reports to third parties a patient's compliance with various medication treatment regimens. Medication containers are provided with electromagnetic tags that provide various information about medicament contained within a respective container. A Smart Tray is equipped with a processor and reader that interrogates each respective electromagnetic tag to identify medicament(s) contained within each container. Using the retrieved information, a Smart Tray provides visual and/or audio signals to a patient to remind the patient when and how much of various medicaments to take. A Smart Tray also monitors, via the reader, when a medication container is removed. A Smart Tray can communicate with one or more third parties, such as healthcare providers, pharmacies, and other suppliers of healthcare products and services via a computer network.
    Type: Grant
    Filed: December 29, 1999
    Date of Patent: September 25, 2001
    Assignee: Becton, Dickinson and Company
    Inventors: Paul Yarin, Richard Fletcher, Joseph DiPisa, Glenn Philander Vonk
  • Patent number: 6291176
    Abstract: Disclosed herein is a newly-identified DNA sequence from Mycobacterium kansasii designated KATS2. Also disclosed are methods, oligonucleotide probes, amplification primers, and kits for the detection of M. kansasii nucleic acids. M. kansasii-specific methods, probes, amplification primers, and kits are preferred.
    Type: Grant
    Filed: June 18, 1999
    Date of Patent: September 18, 2001
    Assignee: Becton, Dickinson and Company
    Inventors: James M. Harris, Qimin You
  • Patent number: 6130047
    Abstract: Detector nucleic acids are employed for detection of nucleic acid target sequences by fluorescence quenching mechanisms. The detector nucleic acid comprises at least two oligonucleotides and is partially single-stranded and partially double-stranded. One of the two dyes of a donor/acceptor dye pair is linked to the first oligonucleotide and the other is linked to a second oligonucleotide such that they are in close spatial proximity when the first and second oligonucleotides are base-paired and donor fluorescence is quenched. A single second oligonucleotide may be hybridized to the first oligonucleotide or multiple second oligonucleotides may be hybridized to the first oligonucleotide and to each other, forming a junction structure comprising multiple donor/acceptor dye pairs. The detector oligonucleotide retains its partially single-stranded and partially double-stranded conformation in the absence of target.
    Type: Grant
    Filed: January 22, 1999
    Date of Patent: October 10, 2000
    Assignee: Beckon, Dickson and Company
    Inventors: James G. Nadeau, Helen V. Hsieh, J. Bruce Pitner, C. Preston Linn
  • Patent number: 6116081
    Abstract: The present invention is directed to an apparatus for measuring gas permeation through test articles, such as evacuated tubes used for blood sample collection. The apparatus includes first and second sealable chambers, with the collection tube being placed in the first chamber. The apparatus is pressurized, and both chambers are sealed such that each chamber has an identical pressure and isolated from the other chamber. Permeation of gas into the collection tube located in the first chamber is detected as a differential pressure change relative to the second chamber. This change in pressure is recorded and is indicative of the rate of gas transport into the collection tube.
    Type: Grant
    Filed: November 21, 1997
    Date of Patent: September 12, 2000
    Assignee: Becton, Dickinson and Company
    Inventor: Jamshed B. Ghandhi
  • Patent number: 6114518
    Abstract: The present invention provides labelled phosphoramidite compositions, methods for making these labelled phosphoramidite compositions, and methods for using these labelled phosphoramidite compositions for labelling oligonucleotides. Even more particularly, the present invention provides compositions and methods for labeling the 5' end of oligonucleotides during synthesis of the oligonucleotides.
    Type: Grant
    Filed: September 30, 1999
    Date of Patent: September 5, 2000
    Assignee: Becton, Dickinson and Company
    Inventors: J. Bruce Pitner, C. Preston Linn
  • Patent number: 6074369
    Abstract: A device for delivering a liquid therapeutic preparation into the body of a patient by injection into or through the skin of the patient comprises a low-profile housing having a bottom surface adapted to be brought into contact with the skin of the patient. A reservoir is disposed within the housing for containing a liquid therapeutic preparation to be administered. An injection needle is disposed generally horizontally in the housing, and is adapted to communicate with the reservoir. The injection needle has a bent injection end which is adapted to project through a needle aperture in the bottom surface of the housing. A movable needle carrier is disposed in the housing for carrying the injection needle and for causing the injection end of the needle to project through the needle aperture upon movement of the needle carrier.
    Type: Grant
    Filed: May 7, 1999
    Date of Patent: June 13, 2000
    Assignee: Becton, Dickinson and Company
    Inventors: Burton H. Sage, Robert I. Connelly
  • Patent number: 6066458
    Abstract: Methods for determining quantities of nucleic acid sequences in samples undergoing amplification utilize amplification ratio estimates (R*) in operations to accurately perform absolute quantitation even when amplification factors for the target and control sequences undergoing amplification are different, time dependent or vary as a function of starting concentrations of nucleic acid sequences. These operations also take into account conversion efficiencies associated with the conversion of probes upon generation of target or control amplicons, but do not require the explicit calculation of such efficiencies. The operations also recognize that a preferred R* should be determined based on a preferred statistical criterion to improve quantitation. In addition, the use of standard samples having known starting concentrations of target and control sequences therein may enable accurate absolute quantitation without the explicit calculation of amplification ratio estimates.
    Type: Grant
    Filed: May 18, 1998
    Date of Patent: May 23, 2000
    Assignee: Becton, Dickinson and Company
    Inventors: Perry D. Haaland, James G. Nadeau, Colleen M. Nycz, Cheryl H. Dean, Catherine A. Spargo
  • Patent number: 6017695
    Abstract: A human brain glycoprotein homologous to the mouse F3 and the chicken contactin/F11 adhesion molecules, nucleic acid sequences encoding the human brain glycoprotein and antibodies directed against the human brain glycoprotein.
    Type: Grant
    Filed: March 26, 1993
    Date of Patent: January 25, 2000
    Assignee: Becton Dickinson and Company
    Inventors: Robert Alan Reid, John Jacob Hemperly
  • Patent number: 6013510
    Abstract: Disclosed herein is a newly-identified DNA sequence from Mycobacterium kansasii designated KATS2. Also disclosed are methods, oligonucleotide probes, amplification primers, and kits for the detection of M. kansasii nucleic acids. M. kansasii-specific methods, probes, amplification primers, and kits are preferred.
    Type: Grant
    Filed: September 25, 1997
    Date of Patent: January 11, 2000
    Assignee: Becton, Dickinson and Company
    Inventors: James M. Harris, Qimin You
  • Patent number: 5985569
    Abstract: Disclosed herein are oligonucleotide primers and probes that can be used to detect species of medical interest in the genus Mycobacterium. Also disclosed are genus-specific methods and kits for detecting Mycobacterium using the disclosed oligonucleotide primers and probes. Preferably, Mycobacterium are detected by amplifying the Mycobactenium nucleic acids using the disclosed amplification primers, and then detecting the amplified nucleic acids. In a more preferred embodiment, Mycobacterium nucleic acids are amplified and detected by thermophilic strand displacement amplification.
    Type: Grant
    Filed: September 26, 1997
    Date of Patent: November 16, 1999
    Assignee: Becton, Dickinson and Company
    Inventors: Paul A. Foxall, Harish Kumar
  • Patent number: 5958700
    Abstract: A detector oligonucleotide having a sequence which forms an intramolecularly base-paired secondary structure is described for use in detecting nucleic acid target sequences and target sequence amplification. The detector oligonucleotide is further modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned on the detector oligonucleotide such that they are in close spatial proximity in the base-paired, folded secondary structure, thereby causing quenching of donor fluorescence. The detector oligonucleotide may optionally further comprise a restriction endonuclease recognition site (RERS) which remains partially or entirely single-stranded in the base-paired secondary structure. The RERS is flanked by the two dyes. In the presence of target, the base-paired secondary structure is unfolded or linearized, increasing the distance between the donor and acceptor dyes and causing a change in fluorescence of the donor and/or the acceptor.
    Type: Grant
    Filed: January 26, 1999
    Date of Patent: September 28, 1999
    Assignee: Becton, Dickinson and Company
    Inventors: James G. Nadeau, J. Bruce Pitner, C. Preston Linn, James L. Schram
  • Patent number: 5957895
    Abstract: A device for delivering a liquid theraputic preparation into the body of a patient by injection into or through the skin of the patient comprises a low-profile housing having a bottom surface adapted to be brought into contact with the skin of the patient. A reservoir is disposed within the housing for containing a liquid theraputic preparation to be administered. An injection needle is disposed generally horizontally in the housing, and is adapted to communicate with the reservoir. The injection needle has a bent injection end which is adapted to project through a needle aperture in the bottom surface of the housing. A movable needle carrier is disposed in the housing for carrying the injection needle and for causing the injection end of the needle to project through the needle aperture upon movement of the needle carrier.
    Type: Grant
    Filed: February 20, 1998
    Date of Patent: September 28, 1999
    Assignee: Becton Dickinson and Company
    Inventors: Burton H. Sage, Robert I. Connelly
  • Patent number: 5935791
    Abstract: Detector nucleic acids are employed for detection of nucleic acid target sequences by fluorescence quenching mechanisms. The detector nucleic acid comprises at least two oligonucleotides and is partially single-stranded and partially double-stranded. One of the two dyes of a donor/acceptor dye pair is linked to the first oligonucleotide and the other is linked to a second oligonucleotide such that they are in close spatial proximity when the first and second oligonucleotides are base-paired and donor fluorescence is quenched. A single second oligonucleotide may be hybridized to the first oligonucleotide or multiple second oligonucleotides may be hybridized to the first oligonucleotide and to each other, forming a junction structure comprising multiple donor/acceptor dye pairs. The detector oligonucleotide retains its partially single-stranded and partially double-stranded conformation in the absence of target.
    Type: Grant
    Filed: September 23, 1997
    Date of Patent: August 10, 1999
    Assignee: Becton, Dickinson and Company
    Inventors: James G. Nadeau, Helen V. Hsieh, J. Bruce Pitner, C. Preston Linn
  • Patent number: 5928869
    Abstract: A detector oligonucleotide having a sequence which forms an intramolecularly base-paired secondary structure is described for use in detecting nucleic acid target sequences and target sequence amplification. The detector oligonucleotide is further modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned on the detector oligonucleotide such that they are in close spatial proximity in the base-paired, folded secondary structure, thereby causing quenching of donor fluorescence. The detector oligonucleotide may optionally further comprise a restriction endonuclease recognition site (RERS) which remains partially or entirely single-stranded in the base-paired secondary structure. The RERS is flanked by the two dyes. In the presence of target, the base-paired secondary structure is unfolded or linearized, increasing the distance between the donor and acceptor dyes and causing a change in fluorescence of the donor and/or the acceptor.
    Type: Grant
    Filed: May 30, 1997
    Date of Patent: July 27, 1999
    Assignee: Becton, Dickinson and Company
    Inventors: James G. Nadeau, J. Bruce Pitner, C. Preston Linn, James L. Schram
  • Patent number: 5919630
    Abstract: Single-stranded signal primers are modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned in sufficiently close spatial proximity on the signal primer that the fluorescence of the first dye is quenched by the second dye. The signal primer may further comprise a restriction endonuclease recognition site (RERS) between the two dyes. As the signal primer is initially single-stranded and remains single-stranded in the absence of target, the restriction endonuclease recognition site is not cleavable or nickable by the restriction endonuclease. In the presence of target, however, signal primer and the restriction endonuclease recognition site are rendered double-stranded and cleavable or nickable by the restriction endonuclease. Cleavage or nicking separates the two dyes and a change in fluorescence due to decreased quenching is detected as an indication of the presence of the target sequence or of target sequence amplification.
    Type: Grant
    Filed: November 4, 1998
    Date of Patent: July 6, 1999
    Assignee: Becton, Dickinson and Company
    Inventors: James G. Nadeau, J. Bruce Pitner, James L. Schram, C. Preston Linn, Glenn P. Vonk, G. Terrance Walker
  • Patent number: 5916779
    Abstract: Strand Displacement Amplification has been adapted to reverse transcription amplification of RNA targets. The method of the invention is referred to as reverse transcription SDA (rtSDA) and may be performed as a two-step process or as a one-step process in which cDNA copies of an RNA target sequence are generated and amplified concurrently. In the one-step process, rtSDA reaction conditions are such that a conventional reverse transcriptase and a DNA-dependent polymerase function together in a single reaction to produce cDNA and amplify it.
    Type: Grant
    Filed: May 8, 1997
    Date of Patent: June 29, 1999
    Assignee: Becton, Dickinson and Company
    Inventors: Robert E. Pearson, Julie A. Dickson, Majid Mehrpouyan
  • Patent number: 5888795
    Abstract: A novel uracil DNA glycosylase enzyme (referred to as Bpa UDG) has been identified in Bacillus pallidus and the gene encoding Bpa UDG has been cloned, sequenced and expressed to produce a recombinant UDG protein. The enzyme is thermostable and exhibits reaction kinetics similar to E. coli UDG. It is effectively inhibited by B. subtilis UGI.Bpa UDG may be used to inactivate contaminating amplicons in nucleic acid amplification reactions, particularly at higher reaction temperatures. It may also be used to generate Bpa UDG-specific antibodies for purification of Bpa-UDG or for detecting Bpa UDG in a sample. Certain Bpa UDG antibodies may inactivate the enzyme and may therefore be useful as substitutes for UGI or heat, or in combination with UGI and/or heat, for controlling UDG activity in a reaction.
    Type: Grant
    Filed: September 9, 1997
    Date of Patent: March 30, 1999
    Assignee: Becton, Dickinson and Company
    Inventor: Paul T. Hamilton
  • Patent number: 5888739
    Abstract: G-quartet structures have been found to be useful in fluorescence assays to detect a selected nucleic acid sequence. When oligonucleotides containing these structures are labeled with a donor fluorophore and an acceptor dye, the folding or interaction of the oligonucleotides in the G-quartet structure brings the donor-acceptor pair into close proximity, allowing an interaction between the two labels which results in quenching of donor fluorescence or a change in other fluorescence properties which are the result of the interaction of two dyes in close proximity. The G-quartet structure unfolds or is otherwise disrupted upon hybridization to its complementary sequence, increasing the distance between the two dye labels. This results in decreased donor quenching or a change in another proximity-related fluorescence parameter.
    Type: Grant
    Filed: September 9, 1997
    Date of Patent: March 30, 1999
    Assignee: Becton, Dickinson and Company
    Inventors: J. Bruce Pitner, James G. Nadeau, Glenn P. Vonk