Abstract: Disclosed herein are phenol oxidizing enzymes obtainable from species of Stachybotrys which are useful in modifying the color associated with dyes and colored compounds, as well as in anti-dye transfer applications. Also disclosed herein are biologically-pure cultures of strains of the genus Stachybotrys, designated herein Stachybotrys parvispora MUCL 38996 and Stachybotrys charatarum MUCL 38898, which are capable of naturally-producing the novel phenol oxidizing enzymes. Disclosed herein is the amino acid and nucleic acid sequence for Stachybotrys phenol oxidizing enzymes as well as expression vectors and host cells comprising the nucleic acid. Disclosed herein are methods for producing the phenol oxidizing enzyme as well as methods for constructing expression host. Disclosed herein are enzyme compositions comprising phenol oxidizing enzymes obtainable from species of Stachybotrys.

Abstract: Described herein are methods for generating recombined nucleic acids. In one method, fragments of a sequence are provided wherein the fragments have non-extendable 3? ends. A primer is provided and the primer and fragments are reacted under conditions to extend the primer to form a recombined nucleic acid molecule. In the methods herein, the non-extendable fragments act only as templates, rather than as templates and primers.

Abstract: Described herein are methods and compositions for the production and secretion of polypeptides. Included herein is the use of interrupting peptide transport activity for an increase in polypeptide production and/or secretion.

Abstract: The present invention provides a mutant aprE promoter and methods for the production of a desired protein in a Bacillus host cell, which comprises the mutant aprE promoter. The present invention provides the sequence of a preferred aprE mutant promoter, which provided for a 100-fold increase in the production of a protein from Bacillus subtilis.

Abstract: Granules are prepared containing an admixture of protein and salt layered over an inert particle. A preferred amount of salt is about between 63.7 and 84.3% of the total weight of the admixture. Proteins include pharmaceutically important proteins such as hormones, or industrially important proteins such as enzymes including proteases, amylases, lipases and cellulases capable of hydrolyzing substrates such as stains. Inert particles include inorganic salts, sugars, sugar alcohols, small organic molecules such as organic acids or salts, and minerals such as clays or silicates. A binder such as starch or polyethylene oxide may be mixed in with the admixture. A barrier material such as an inorganic salt or organic acid or salt may be in the admixture or coated over the admixture layer. A coating layer of a soluble or water dispersible film-forming polymer may be between the inert particle and admixture layer and/or over the admixture layer.

Abstract: The invention relates to phenol oxidizing enzymes encoded by nucleic acids capable of hybridizing to the nucleic acid having the sequence shown in SEQ ID NO: 1 and in particular phenol oxidizing enzymes obtainable from fungus. Particularly provided are nucleic acid sequences and amino acids from Bipolaris spicifera, Curvularia pallescens and Amerosporium atrum, and expression vectors and host cells comprising said nucleic acid sequences encoding phenol oxidizing enzymes. Additionally, methods for producing the phenol oxidizing enzymes as well as methods for constructing expression hosts are disclosed.

Abstract: Improved methods of treating cellulose containing fabrics with cellulase comprising contacting the cellulose fabrics with truncated cellulase enzyme. Treatment of cellulose containing fabrics with cellulase core domains of the invention are disclosed as offering specific advantages of reduced redeposition of dye and increased abrasion.