Patents Represented by Attorney Kristin J. McNamara
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Patent number: 8349594Abstract: The present invention relates to a process for enzymatic lipase interesterification of oils containing a chelating agent by sequential or simultaneous treatment with a base.Type: GrantFiled: September 11, 2006Date of Patent: January 8, 2013Assignees: Novozymes A/S, Novozymes North America, Inc.Inventors: Steven White Pearce, Lars Saaby Pedersen, Hans Christian Holm, Tommy Lykke Husum, Per Munk Nielsen
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Patent number: 8329632Abstract: New detergent compositions and the use of enzyme combinations therein are disclosed. The compositions have enhanced stability of non protease enzymes present in the compositions.Type: GrantFiled: August 17, 2010Date of Patent: December 11, 2012Assignee: Novozymes A/SInventors: Mikael Mikkelsen, Niels Munk Ryom, Claus Ladefoged, Sandra Friis-Jensen
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Patent number: 8318470Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: GrantFiled: January 5, 2012Date of Patent: November 27, 2012Assignee: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
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Patent number: 8309337Abstract: The inventors realized that the diversity generated by conventional methods may be limited by steric hindrance between amino acid residues in the three-dimensional structures of the resulting polypeptides. The steric hindrance may occur between amino acid residues at widely different positions in the amino acid sequences, e.g. between residues in two different domains of the 3D structure, and resulting polypeptides which include such steric hindrance may never be observed in the conventional recombination methods because they may be expressed in poor yields or may have poor activity or stability. The inventors developed a method to identify and alleviate such steric hindrance in the resulting polypeptides. In an alignment of the three-dimensional structures, steric hindrance is indicated when residues from two different structures are located within a certain distance.Type: GrantFiled: August 3, 2011Date of Patent: November 13, 2012Assignee: Novozymes A/SInventors: Allan Svendesn, Lars Beier
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Patent number: 8309147Abstract: A method of screening lipolytic enzymes is used to identify candidates for a baking additive which can improve the properties of a baked product when added to the dough. The screening method involves contacting the enzyme with N-acyl phosphatidyl ethanolamine (APE) or N-acyl lyso-phosphatidyl ethanolamine (ALPE), and detecting hydrolysis of an ester bond in the APE or ALPE.Type: GrantFiled: December 11, 2003Date of Patent: November 13, 2012Assignee: Novozymes A/SInventors: Christel Thea Jorgensen, Luise Erlandsen, Kim Borch, Jesper Vind
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Patent number: 8298800Abstract: The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.Type: GrantFiled: November 4, 2009Date of Patent: October 30, 2012Assignee: Novozymes A/SInventors: Kim Borch, Luise Erlandsen, Jesper Vind, Allan Svendsen, Christel Thea Jorgensen
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Patent number: 8288510Abstract: The invention provides for isolated peptides having phospholipase inhibitory activity, polypeptides comprising phospholipase inhibitory activity and lipases capable of being inhibited by the isolated peptides and/or polypeptides comprising phospholipase inhibitory activity. The invention also relates to nucleic acid constructs, recombinant expression vectors, and recombinant host cells comprising the polynucleotides as well as methods for producing and using the peptides and the polypeptides having lipase inhibitory activity.Type: GrantFiled: April 30, 2009Date of Patent: October 16, 2012Assignee: Novozymes A/SInventors: Leonardo de Naria, Ming Li, Christian Isak Joergensen, Kim Borch, Tom Anton Busk Nielsen, Jesper Vind
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Patent number: 8283149Abstract: The present invention relates to a new wild-type lipase, its nucleic acid sequences and the amino acid sequences thereof. This new lipase shows washing performance in the form of wild-type. The present invention also relates to the method for producing the lipase and the uses of the lipase in baking.Type: GrantFiled: June 22, 2010Date of Patent: October 9, 2012Assignee: Novozymes A/SInventors: XiaoMei Niu, Ye Liu, Jurgen Carsten Franz Knotzel, Signe Munk
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Patent number: 8273348Abstract: The pharmaceutical use of lipases related to the Thermomyces lanuginosus (Humicola lanuginosa) lipase comprising amino acids 1-269 of SEQ ID NO: 2, optionally in combination with a protease and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The lipases of the invention have, e.g., an improved digestion performance in vitro, an improved activity at a pH in the neutral range, an improved stability at low pH, an are stable against protease-degradation, and/or are stable in the presence of pepsin and bile salts. The invention also relates to methods of determining digestion performance in vitro of lipases, as well as to certain novel variants of the lipase of T. lanuginosus.Type: GrantFiled: December 12, 2007Date of Patent: September 25, 2012Assignees: Novozymes A/S, Novozymes Inc.Inventors: Allan Svendsen, Michael Skjoet, Debbie Yaver, Lars Lehmann Hylling Christensen, Signe Eskildsen Larsen, Nina Lundin, Michael Lamsa, Peter Colin Gregory
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Patent number: 8263376Abstract: The present disclosure relates to an isolated lipoxygenase. The lipoxygenase of the present disclosure includes polypeptide with at least 80% sequence homology to the mature peptide shown in SEQ ID NO: 2. Lipoxygenase of the present disclosure is suitable for use in, among other things, baking and in detergent compositions.Type: GrantFiled: March 31, 2011Date of Patent: September 11, 2012Assignee: Novozymes A/SInventors: Akiko Sugio, Shinobu Takagi
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Patent number: 8232087Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: GrantFiled: February 9, 2011Date of Patent: July 31, 2012Assignee: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup
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Patent number: 8232086Abstract: The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.Type: GrantFiled: January 20, 2011Date of Patent: July 31, 2012Assignee: Novozymes A/SInventors: Joel Cherry, Allan Svendsen, Carsten Andersen, Lars Beier, Torben Peter Frandsen
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Patent number: 8226995Abstract: The invention relates to a method for producing fractions of a milk composition by treating a milk composition with a phospholipase and separating it into at least two fractions with different fat content. The fractions obtained have improved properties for producing food products. The invention further relates to a method for producing food products from one or more of the fractions obtained. In further aspects the invention relates to methods for producing condensed milk, milk powder, butter and dairy spread from a milk composition treated with a phospholipase.Type: GrantFiled: December 21, 2005Date of Patent: July 24, 2012Assignee: Novozymes A/SInventors: Per Munk Nielsen, Hanna Lilbaek
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Patent number: 8222372Abstract: The present invention relates to a method of producing a whey protein hydrolysate using a microbial endopeptidase which specifically cleaves on the carboxy terminal side of arginine or lysine. The invention also relates to use of such whey protein hydrolysate in sports drinks or in clinical nutrition.Type: GrantFiled: April 15, 2008Date of Patent: July 17, 2012Assignee: Novozymes A/SInventors: Gitte Budolfsen, Per Munk Nielsen
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Patent number: 8187854Abstract: The invention provides variant lipases, preferably, variants with reduced tendency to odor generation obtained by introducing mutations in one or more regions identified in a parent lipase.Type: GrantFiled: January 22, 2007Date of Patent: May 29, 2012Assignee: Novozymes A/SInventors: Jesper Vind, Jürgen Carsten Franz Knötzel, Kim Borch, Allan Svendsen, Thomas Honger Callisen, Debbie Yaver, Mads Eskelund Bjornvad, Peter Kamp Hansen, Haiyan Ge, Michael Lamsa
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Patent number: 8084241Abstract: The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN? subtilase and to TY145 and BPN? variants having altered properties as compared to the parent TY145/BPN? subtilase.Type: GrantFiled: September 10, 2010Date of Patent: December 27, 2011Assignee: Novozymes A/SInventors: Allan Svendsen, Henriette Draborg
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Patent number: 8017371Abstract: The inventors realized that the diversity generated by conventional methods may be limited by steric hindrance between amino acid residues in the three-dimensional structures of the resulting polypeptides. The steric hindrance may occur between amino acid residues at widely different positions in the amino acid sequences, e.g. between residues in two different domains of the 3D structure, and resulting polypeptides which include such steric hindrance may never be observed in the conventional recombination methods because they may be expressed in poor yields or may have poor activity or stability. The inventors developed a method to identify and alleviate such steric hindrance in the resulting polypeptides. In an alignment of the three-dimensional structures, steric hindrance is indicated when residues from two different structures are located within a certain distance.Type: GrantFiled: February 11, 2010Date of Patent: September 13, 2011Assignee: Novozymes A/SInventors: Allan Svendesn, Lars Beier
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Patent number: 8012724Abstract: The present invention relates to a method for producing fatty acid alkyl esters, such as fatty acid methyl esters (FAME) and fatty acid ethyl esters with a low level of impurities such as phospholipids. The method of the invention is simplified by combining two process steps into one single process step and is therefore economically cheaper. The method includes mixing water, alcohol, triglyceride and/or free fatty acids a lipolytic enzyme and a phospholipase. Subsequently the aqueous phase, which contains glycerine, residual enzyme and most of the hydrolyzed phospholipids, is separated from the non-aqueous phase, whereby the content of phospholipids in the non-aqueous phase is reduced.Type: GrantFiled: June 13, 2006Date of Patent: September 6, 2011Assignee: Novozymes A/SInventors: Hans Christian Holm, Per Munk Nielsen, Morten Wurtz Christensen
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Patent number: 8008057Abstract: The present invention relates to methods for producing variants of a parent JP170 subtilase and of a parent BPN? subtilase and to JP170 and BPN? variants having altered properties as compared to the parent JP170/BPN? subtilase.Type: GrantFiled: March 10, 2009Date of Patent: August 30, 2011Assignee: Novozymes A/SInventors: Allan Svendsen, Stefan Minning
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Patent number: 7993900Abstract: The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.Type: GrantFiled: August 16, 2010Date of Patent: August 9, 2011Assignee: Novozymes A/SInventors: Preben Nielsen, Poul Erik Pedersen, Helle Outtrup