Patents Represented by Attorney Stacy R. Sias
  • Patent number: 5641864
    Abstract: Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided.
    Type: Grant
    Filed: September 22, 1994
    Date of Patent: June 24, 1997
    Assignee: Hoffman-La Roche Inc.
    Inventor: David H. Gelfand