Patents Assigned to Ajinomoto Co., Inc.
  • Publication number: 20210139541
    Abstract: Compounds having an affinity substance to an antibody and a bioorthogonal functional group, represented by the following Formula (I): A-L-E-B??(I) wherein A is an affinity substance to an antibody, L is a divalent group comprising a leaving group, E is a divalent group comprising an electrophilic group (i) coupled with the leaving group and (ii) having ability to react with a nucleophilic group in the antibody, B is a bioorthogonal functional group, and the leaving group has ability to be cleaved and eliminated from E by a reaction between the nucleophilic group and the electrophilic group, or a salt thereof, and the like are useful for labelling antibodies.
    Type: Application
    Filed: December 11, 2020
    Publication date: May 13, 2021
    Applicant: Ajinomoto Co., Inc.
    Inventors: Yutaka MATSUDA, Noriko HATADA, Kei YAMADA, Natsuki SHIKIDA, Kazutaka SHIMBO, Tomohiro FUJII, Shigeo HIRASAWA
  • Publication number: 20210139549
    Abstract: Compounds having an affinity substance to an antibody, a cleavable portion, and a reactive group, represented by the following Formula (I): A-L-B-R??(I) wherein A is the affinity substance an antibody, L is a cleavable linker which is a divalent group comprising the cleavable portion, B is (a) a divalent group comprising a bioorthogonal functional group or (b) a divalent group comprising no bioorthogonal functional group, and R is the reactive group to the antibody, in which the affinity substance to an antibody is a certain peptide, or a salt thereof are useful for the modification of an antibody, particularly the regioselective modification of an antibody.
    Type: Application
    Filed: December 11, 2020
    Publication date: May 13, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Kei YAMADA, Tomohiro FUJII, Natsuki SHIKIDA, Kazutaka SHIMBO
  • Publication number: 20210115397
    Abstract: In order to improve the efficiency of inducing differentiation of pluripotent stem cells, provided is a method for promoting differentiation of pluripotent stem cells, the method including a step of culturing pluripotent stem cells in a medium, wherein the medium is a medium containing a) an insulin-like growth factor, b) an insulin analogue preparation containing no zinc, c) an insulin analogue preparation containing a low concentration of zinc, or d) a compound exhibiting an insulin-like action.
    Type: Application
    Filed: April 25, 2019
    Publication date: April 22, 2021
    Applicants: TOKYO INSTITUTE OF TECHNOLOGY, Ajinomoto Co., Inc.
    Inventors: Shoen KUME, Nobuaki SHIRAKI, Akira CHIBA, Takayuki ENOMOTO
  • Publication number: 20210115484
    Abstract: A method for enzymatic sulfurylation of a substrate is provided which includes the steps of reacting the substrate with 3?-phosphoadenosine-5?-phosphosulfate (PAPS) in a medium containing a bacterium belonging to the family Enterobacteriaceae to produce a sulfated derivative of the substrate, and collecting the sulfated derivative from the medium, wherein the bacterium has been modified to produce, at least, a protein having sulfotransferase activity, and to attenuate expression of an aphA gene, a cysQ gene, or a cpdB gene, or a combination of these.
    Type: Application
    Filed: December 23, 2020
    Publication date: April 22, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Sergey Vasilievich Smirnov, Irina Lvovna Tokmakova
  • Publication number: 20210112817
    Abstract: Frozen gyoza dumplings that can afford a gyoza dumpling having good wings (gyoza dumpling with wing) that easily spread on a cooking device when heating with the cooking device, etc., do not easily break during peeling off from the cooking device after cooking, and do not easily soften when the gyoza dumpling is placed with the fried surface facing upward are provided by attaching a composition thereto in a frozen state, the composition containing a modified starch having a viscosity of less than 5 mPa·s as measured by method A defined in the present specification and a protein.
    Type: Application
    Filed: December 23, 2020
    Publication date: April 22, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Shogo HIRAE, Kohei KAWAMURA
  • Publication number: 20210106031
    Abstract: Adding (a) transglutaminase, and a gluconic acid salt or lipase to a processed meat food product, or (b) transglutaminase, a gluconic acid salt, and lipase to a processed meat food product with a sodium chloride content of 0.1 wt % to 1 wt % or a processed meat food product with a binding agent content of not more than 0.2 wt % affords a processed meat food product with a reduced sodium chloride content or a reduced binding agent content, with a good elastic texture.
    Type: Application
    Filed: December 21, 2020
    Publication date: April 15, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Takuya OHIRA, Hiroyuki NAKAGOSHI, Fumiyuki HIROSE, Hiroshi TAKAYANAGI
  • Patent number: 10968429
    Abstract: Culture media, which contain chelated iron, promote cell proliferation of neural stem cells and/or neural progenitor cells while maintaining undifferentiated state and multipotency.
    Type: Grant
    Filed: September 28, 2017
    Date of Patent: April 6, 2021
    Assignee: AJINOMOTO CO., INC.
    Inventors: Takuya Matsumoto, Sho Senda, Tsuyoshi Kobayashi, Akihiro Arakawa
  • Publication number: 20210093601
    Abstract: Compositions containing one or more kinds of essential amino acids other than leucine and not less than 35 mol % of leucine, relative to the total content of essential amino acids, s are useful for preventing or improving dementia or a depressive state, in particular, a depressive state caused by stress, have high safety, and can be continuously ingested or administered.
    Type: Application
    Filed: December 14, 2020
    Publication date: April 1, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventor: Shinobu NISHITANI
  • Patent number: 10962547
    Abstract: An evaluating method includes an evaluating step of evaluating a state of ketosis in postpartum dairy cows for a dairy cow using at least one value of concentration values of Ala, Arg, Asn, Asp, BCAA, Cit, Cys, Glu, Gln, Gly, His, Ile, Leu, Lys, Met, 3MeHis, Orn, Phe, Pro, Ser, Tau, Thr, Trp, Tyr, and Val and concentration values of ALB, ALT, AST, BHBA, BUN, Ca, gGTP, Glc, NEFA, T-Bil, TCHO, TG, and TP in blood of the dairy cow before parturition.
    Type: Grant
    Filed: December 26, 2018
    Date of Patent: March 30, 2021
    Assignee: Ajinomoto Co., Inc.
    Inventors: Takashi Mikami, Akira Imaizumi, Takayuki Tanaka, Yuki Miyazawa, Mina Nakamura, Kazuki Nakagawa, Takeshi Fujieda
  • Patent number: 10961560
    Abstract: A mutated tryptophan oxidase suitable for practical implementation is described herein. Specifically, a mutated tryptophan oxidase wherein at least one amino acid residue of a wild-type tryptophan oxidase is mutated and, as a result, has higher tryptophan oxidase activity and/or stability as compared to the wild-type tryptophan oxidase. The mutated tryptophan oxidase can be derived from a wild-type tryptophan oxidase having at least one of Motifs (2), (3), (5), (7), (9), (11), (13), and (14), and at least one amino acid residue in any of these motifs can have mutation. The mutated tryptophan oxidase also can have a mutation of one or more amino acid residues in an amino acid sequence represented by SEQ ID NO: 2 and a sequence homologous thereto.
    Type: Grant
    Filed: March 6, 2019
    Date of Patent: March 30, 2021
    Assignee: AJINOMOTO CO., INC.
    Inventors: Hiroki Yamaguchi, Kazutoshi Takahashi, Moemi Tatsumi
  • Patent number: 10947508
    Abstract: Mesenchymal stem cells may be culture for a long period, without using any special apparatus, equipment and the like, in a medium in which seven kinds of nonessential amino acids of glycine, alanine, serine, proline, asparagine, aspartic acid, and glutamic acid are reduced.
    Type: Grant
    Filed: February 17, 2017
    Date of Patent: March 16, 2021
    Assignees: AJINOMOTO CO., INC., Kyoto University
    Inventors: Yonghui Jin, Ikue Harata, Sho Senda, Hiroki Ozawa, Tsuyoshi Kobayashi, Junya Toguchida, Makoto Ikeya
  • Publication number: 20210071214
    Abstract: A method efficiently produces a compound containing an N-acyl-amino group by an enzymatic method. Specifically, a method of producing a compound containing an N-acyl-amino group includes producing the compound containing an N-acyl-amino group by reacting a compound containing an amino group with a compound containing a carboxyl group in the presence of an enzyme having an ability to bond a carboxyl group and an amino group in an ATP dependent manner to form an amide bond.
    Type: Application
    Filed: September 18, 2020
    Publication date: March 11, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Hidemi Nagata, Keiko Danjou, Jun Takakura, Hiroyuki Nozaki
  • Publication number: 20210071210
    Abstract: The present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, productivity of which has been enhanced. Specifically, the present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, by producing 13-hydroxy-9(Z)-octadecenoic acid from linoleic acid in the presence of a transformed microorganism that produces a protein such as the following: (A) a protein having an amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14; (B) a protein having an amino acid sequence containing one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14, and having a linoleate 13-hydratase activity; and (C) a protein having an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13 or 14, and having a linoleate 13-hydratase activity.
    Type: Application
    Filed: September 29, 2020
    Publication date: March 11, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Yoko Mihara, Yohei Tatsukami, Yoshinori Tajima
  • Publication number: 20210070800
    Abstract: A method for producing ?-Glu-Val-Gly is described, wherein the method includes the steps of cultivating a ?-Glu-Val-Gly-producing bacterium belonging to the family Enterobacteriaceae in a culture medium so that the ?-Glu-Val-Gly accumulates in the culture medium or the cells of the bacterium, or both, and collecting the ?-Glu-Val-Gly from the culture medium or the cells of the bacterium, or both. The bacterium has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity.
    Type: Application
    Filed: November 20, 2020
    Publication date: March 11, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Natalia V. STOYNOVA, Elena V. SYCHEVA, Natalia V. GERASKINA, Elena V. MATROSOVA, Sergey V. SMIRNOV, Ayako SATO, Eri HIGASHIURA, Misato OKAMOTO, Takayuki ITO, Erika WATANABE, Yuki ODA, Uno TAGAMI, Tatsuki KASHIWAGI, Masayuki SUGIKI
  • Publication number: 20210068432
    Abstract: A starch-containing food with improved properties may be obtained by reacting an actinomycete-derived amylomaltase with starch in the raw material.
    Type: Application
    Filed: September 25, 2020
    Publication date: March 11, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Kazuto AKAMOTO, Kazumi SUGINO, Noriko YOKOYAMA
  • Patent number: 10941427
    Abstract: A production system for a product selected from a nitrogen-containing product and a fermented and cultured product that does not involve (or can minimize) the transport of liquid ammonia can include: an ammonia synthesis apparatus in which an ammonia-containing gas is synthesized by reaction of a source gas containing hydrogen and nitrogen in the presence of a supported metal catalyst containing as a support one or more selected from the group consisting of: i) a conductive mayenite compound; ii) a two-dimensional electride compound or a precursor thereof; and iii) a complex formed of a support base containing at least one metal oxide selected from ZrO2, TiO2, CeO2, and MgO and a metal amide represented by a formula M(NH2)x (where M represents one or more selected from Li, Na, K, Be, Mg, Ca, Sr, Ba, and Eu; and x represents a valence number of M) supported by the support base.
    Type: Grant
    Filed: August 11, 2017
    Date of Patent: March 9, 2021
    Assignees: Ajinomoto Co., Inc., Tokyo Institute of Technology
    Inventors: Mitsuhiro Kishino, Hiroyuki Kojima, Hideo Hosono, Michikazu Hara, Masaaki Kitano, Toshiharu Yokoyama, Toru Numaguchi, Munenobu Ito, Kazuteru Yamada, Hiromi Noguchi
  • Patent number: 10941390
    Abstract: A novel protein deamidase having an activity of directly acting on a side chain amide group of an asparagine residue in a protein to form a side chain carboxyl group and release ammonia, a microorganism that produces the same, a gene encoding the same, a method for producing the same, and use of the same are provided. A bacterium classified into the class Actinobacteria is cultured to generate protein deamidase, and the enzyme is collected from culture.
    Type: Grant
    Filed: June 10, 2020
    Date of Patent: March 9, 2021
    Assignee: AJINOMOTO CO., INC.
    Inventors: Noriko Yokoyama, Mayu Nakano, Shuhei Hashiro, Masako Hiraga, Kazutaka Shimbo, Ayaka Tokunaga
  • Patent number: 10941420
    Abstract: The present invention provides a composition containing linalool, wherein the composition contains a high amount of either enantiomer R-linalool or S-linalool, and has a high content rate of linalool. The present invention also provides a production method for producing the composition. The present invention further provides a composition containing volatile components including linalool, in which a content of linalool in a total content of the volatile components in the composition is 60% or more, and the linalool is present as R-linalool or S-linalool in an amount of 50% or more of the enantiomer, and a production method therefor.
    Type: Grant
    Filed: March 21, 2018
    Date of Patent: March 9, 2021
    Assignee: AJINOMOTO CO., INC.
    Inventors: Yasushi Hoshino, Yoshiko Inoue, Mika Moriya, Akiko Matsudaira, Yosuke Nishio
  • Publication number: 20210062173
    Abstract: Hemicellulase that degrades corn non-starch polysaccharides (“NSP”), DNA encoding the same, and a method of using the hemicellulase and its DNA are provided. Proteins having hemicellulase activity such as Xyn5A, Xyn10B, Xyn11A, Xyn30A, and Xyn43A are described.
    Type: Application
    Filed: October 30, 2020
    Publication date: March 4, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventors: Yusuke Hagiwara, Yasuhiro Mihara, Tomo Takagi, Satoshi Nakamura, Rie Yakushijin
  • Publication number: 20210054425
    Abstract: The present invention provides a method for producing L-methionine by fermentation using a bacterium belonging to the genus Pantoea which has been modified to overexpress the rarD gene or a mutant gene thereof.
    Type: Application
    Filed: October 27, 2020
    Publication date: February 25, 2021
    Applicant: AJINOMOTO CO., INC.
    Inventor: Mikhail Kharisovich Ziyatdinov