Patents Assigned to AmberGen, Inc.
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Patent number: 8278045Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)dyes.Type: GrantFiled: February 9, 2011Date of Patent: October 2, 2012Assignee: Ambergen, Inc.Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik
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Publication number: 20120244562Abstract: Methods and compositions are described for the diagnosis of primary biliary cirrhosis. Novel autoantigens are described for use in assays which employ test samples from individuals.Type: ApplicationFiled: October 5, 2010Publication date: September 27, 2012Applicants: AMBERGEN, INC., MASSACHUSETTS GENERAL HOSPITIALInventors: Mark J. Lim, Heather P. Ostendorf, Kenneth J. Rothschild, Donald B. Bloch
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Patent number: 8148062Abstract: The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system. The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other mammals, and kits which may used for the detection of diseases and disorders.Type: GrantFiled: December 14, 2006Date of Patent: April 3, 2012Assignee: Ambergen, Inc.Inventors: Kenneth J. Rothschild, Sanjay M. Sonar, Jerzy Olejnik
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Patent number: 8114587Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.Type: GrantFiled: June 23, 2005Date of Patent: February 14, 2012Assignee: Ambergen, Inc.Inventors: Sadanand Gite, Jennifer A. McCullough, Kenneth J. Rothschild
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Patent number: 7897335Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.Type: GrantFiled: June 18, 2002Date of Patent: March 1, 2011Assignee: Ambergen, Inc.Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik
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Patent number: 7807407Abstract: This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as a mass difference.Type: GrantFiled: July 30, 2004Date of Patent: October 5, 2010Assignee: AmberGen, Inc.Inventors: Alex M. Garvin, Sadanand Gite, Vladislav B. Bergo, Kenneth J. Rothschild
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Patent number: 7595198Abstract: This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as proteins and protein fragments from biological samples from in vivo and in vitro sources. Agents comprise a detectable group bound to a photoreactive group. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed.Type: GrantFiled: October 17, 2006Date of Patent: September 29, 2009Assignee: Ambergen, Inc.Inventors: Jerzy Olejnik, Kenneth J. Rothschild
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Patent number: 7563598Abstract: This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as a mass difference.Type: GrantFiled: July 14, 2006Date of Patent: July 21, 2009Assignee: Ambergen, Inc.Inventors: Alex Garvin, Sadanand Gite, Vladislav Bergo, Kenneth Rothschild
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Patent number: 7547530Abstract: Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable markere nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates.Type: GrantFiled: February 9, 2006Date of Patent: June 16, 2009Assignee: AmberGen, Inc.Inventors: Jerzy Olejnik, Edyta Krzymanska-Olejnik, Kenneth J. Rothschild
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Patent number: 7534861Abstract: A hybrid polypeptide composed of a p53 epitope peptide and a desired functional protein are produced by recombinant DNA techniques. A DNA expression vector is constructed that includes segments of DNA coding for the epitope peptide and the desired functional protein. An optional linking portion is contemplated. The linking portion of the epitope peptide is cleavable at a specific amino acid residue adjacent the functional protein by use of a sequence specific proteolytic enzyme or chemical proteolytic agent. The hybrid polypeptide expressed by the host cells transformed by the cloning vector is removed therefrom and purified by affinity chromatography techniques by use of an immobilized antibody specific to the antigenic portion of the epitope peptide. The protein is then cleaved from the isolated hybrid polypeptide with an appropriate proteolic enzyme or chemical agent, thereby releasing the mature functional protein in highly purified, highly active state.Type: GrantFiled: January 10, 2003Date of Patent: May 19, 2009Assignee: Ambergen, Inc.Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik, Mark Lim
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Patent number: 7524941Abstract: The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.Type: GrantFiled: September 11, 2007Date of Patent: April 28, 2009Assignee: Ambergen, Inc.Inventors: Jerzy Olejnik, Edyta Krzymanska-Olejnik, Sergey Mamaev, Kenneth J. Rothschild
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Patent number: 7312060Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride(4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)dyes.Type: GrantFiled: January 10, 2003Date of Patent: December 25, 2007Assignee: Ambergen, Inc.Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik, Mark Lim
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Patent number: 7288372Abstract: The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.Type: GrantFiled: January 16, 2003Date of Patent: October 30, 2007Assignee: Ambergen, Inc.Inventors: Jerzy Olejnik, Edyta Krzymanska-Olejnik, Sergey Mamaev, Kenneth J. Rothschild
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Patent number: 7252932Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.Type: GrantFiled: August 23, 2000Date of Patent: August 7, 2007Assignee: AmberGen, Inc.Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik, Sergey Mamaev, Mark Lim
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Publication number: 20070072214Abstract: This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as a mass difference.Type: ApplicationFiled: July 14, 2006Publication date: March 29, 2007Applicant: AMBERGEN INC.Inventors: Alex Garvin, Sadanand Gite, Vladislav Bergo, Kenneth Rothschild
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Patent number: 7145019Abstract: This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as proteins and protein fragments from biological samples from in vivo and in vitro sources. Agents comprise a detectable group bound to a photoreactive group. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed.Type: GrantFiled: October 16, 2003Date of Patent: December 5, 2006Assignee: Ambergen, Inc.Inventors: Jerzy Olejnik, Kenneth J. Rothschild
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Publication number: 20060252923Abstract: Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable markere nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates.Type: ApplicationFiled: February 9, 2006Publication date: November 9, 2006Applicant: AmberGen, Inc.Inventors: Jerzy Olejnik, Edyta Krzymanska-Olejnik, Kenneth Rothschild
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Patent number: 7101662Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents.Type: GrantFiled: October 9, 2001Date of Patent: September 5, 2006Assignee: Ambergen, Inc.Inventors: Kenneth J. Rothschild, Sadanand Gite, Jerzy Olejnik
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Patent number: 7057031Abstract: Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable markere nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates.Type: GrantFiled: July 12, 2002Date of Patent: June 6, 2006Assignee: AmberGen, Inc.Inventors: Jerzy Olejnik, Edyta Krzymanska-Olejnik, Kenneth J. Rothschild
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Publication number: 20060068449Abstract: This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents.Type: ApplicationFiled: November 19, 2004Publication date: March 30, 2006Applicant: AmberGen, Inc.Inventors: Kenneth Rothschild, Sadanand Gite, Jerzy Olejnik