Patents Assigned to BGI Shenzhen Co.,Limited
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Patent number: 11822629Abstract: A method for generating a digital identity is provided, including: extracting a first preset number of short tandem repeat STRs and relevant information of each STR from whole genome data; generating a single STR digital code corresponding to each STR according to the relevant information of each STR, to obtain a plurality of single STR digital codes; performing sequence transformation on each single STR digital code with a preset rule, and generating a target STR digital code according to the single STR digital code after the sequence transformation; generating summary information of the target STR digital code, and determining the summary information as summary information of the STR to which the target STR digital code belongs; and determining the summary information of the STR as the generated digital identity.Type: GrantFiled: December 15, 2020Date of Patent: November 21, 2023Assignee: BGI SHENZHEN CO., LIMITEDInventors: Guangming Pan, Meng Yang, Hongde Zhao, Qiang Tang, Tong Zhou, Hang Li, Huaping Li
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Patent number: 10858407Abstract: Disclosed are a conotoxin polypeptide ?-CPTx-bt102, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 15 amino acids, has a molecular weight of 1660.61 daltons, and has the full sequence RCRCEQTCGTCVPCC (SEQ. ID NO. 1).Type: GrantFiled: September 30, 2014Date of Patent: December 8, 2020Assignee: BGI Shenzhen Co., LimitedInventors: Zhilong Lin, Bo Wen, Ting Tong, Jie Liu, Chaoqin Du, Fen Mo, Chao Peng, Qiong Shi
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Patent number: 10858406Abstract: Disclosed are a conotoxin polypeptide ?-CPTx-bt105, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 16 amino acids, has a molecular weight of 1626.62 daltons, and has the full sequence GICCVDDTCTTHSGCL (SEQ. ID NO. 1).Type: GrantFiled: September 30, 2014Date of Patent: December 8, 2020Assignee: BGI Shenzhen Co., LimitedInventors: Zhilong Lin, Bo Wen, Ting Tong, Jie Liu, Chaoqin Du, Fen Mo, Chao Peng, Qiong Shi
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Patent number: 10526659Abstract: Biomarkers and methods related to microbiota for predicting the risk of a disease, particularly colorectal cancer (CRC), are described.Type: GrantFiled: February 5, 2016Date of Patent: January 7, 2020Assignees: BGI Shenzhen Co., Limited, BGI ShenzhenInventors: Qiang Feng, Dongya Zhang, Longqing Tang, Jun Wang
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Patent number: 10456769Abstract: Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded with two adaptors to obtain a fragmented product with two adaptors respectively at two ends; and 4) amplifying the fragmented product with two adaptors respectively at two ends using a tag sequence and a pair of primers to obtain said sequencing library.Type: GrantFiled: August 3, 2017Date of Patent: October 29, 2019Assignee: BGI Shenzhen & BGI Shenzhen Co., LimitedInventors: Ou Wang, Xiaofang Cheng, Liangying Zou, Cankun Chang, Hui Jiang, Wenwei Zhang
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Patent number: 10100360Abstract: Biomarkers for diabetes and usages thereof are provided. And the biomarkers are nucleotides having polynucleotide sequences defined in SEQ ID NOs: 1-50.Type: GrantFiled: June 5, 2013Date of Patent: October 16, 2018Assignees: BGI SHENZHEN, BGI SHENZHEN CO., LIMITEDInventors: Shenghui Li, Qiang Feng, Zhuye Jie, Dongya Zhang, Junjie Qin, Jun Wang, Jian Wang, Huanming Yang
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Patent number: 10087216Abstract: Disclosed are a conotoxin polypeptide ?-CPTx-bt101, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 18 amino acids, has a molecular weight of 1872.72 daltons, and has the full sequence KCCTMSVCQPPPVCTCCA (SEQ. ID NO. 1).Type: GrantFiled: September 30, 2014Date of Patent: October 2, 2018Assignee: BGI SHENZHEN CO., LIMITEDInventors: Zhilong Lin, Bo Wen, Ting Tong, Jie Liu, Chaoqin Du, Fen Mo, Chao Peng, Qiong Shi
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Publication number: 20180251813Abstract: Disclosed are a nucleic acid fragmentation method and a sequence combination. The method comprises the following steps: subjecting a denatured nucleic acid to annealing and an extension reaction by using a single-stranded 5?-end extension primer, wherein the single-stranded 5?-end extension primer comprises a sequencing platform adaptor sequence of a 5? end and a connected random sequence, and the random sequence is subjected to annealing on a random site of the denatured nucleic acid; and directionally connecting a double-stranded 3?-end adaptor sequence to the 3? end of the nucleic acid generated in the extension reaction, and carrying out denaturalization and purification to obtain a fragmented single-stranded nucleic acid with adaptor sequences on two ends.Type: ApplicationFiled: October 13, 2014Publication date: September 6, 2018Applicant: BGI SHENZHEN CO., LIMITEDInventors: Hongyan HAN, Chunyu GENG, Guanying Guo, Wenwei ZHANG, Hui JIANG, Yuan JIANG
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Publication number: 20180195060Abstract: Disclosed is a method for constructing a long fragment DNA library, comprising the following steps: 1) breaking a long fragment DNA into target fragments of 3-10 kb by transposase, then amplifying the target fragments, and obtaining target fragment amplification products containing dUTP; 2) amplifying the dUTP in the products by removing the target fragments, fragmenting the target fragments secondarily into DNA short fragments of 300-1200 bp; 3) connecting both ends of the DNA short fragments with sequencing linker single chains A and sequencing linker single chains B respectively; and obtaining connecting sequencing linker products; and 4) PCR amplifying the connecting sequencing linker products, to obtain amplification products.Type: ApplicationFiled: April 14, 2016Publication date: July 12, 2018Applicants: BGI SHENZHEN, BGI SHENZHEN CO., LIMITEDInventors: Ou WANG, Cankun CHANG, Lin LIN, Hui JIANG, Wenwei ZHANG
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Publication number: 20180163251Abstract: Provided are a target region enrichment method based on multiplex PCR, and a reagent, the method comprising: connecting a first linker and a second linker respectively at two ends of a nucleic acid segment containing target regions to be enriched so as to obtain a linker-connected product; performing a PCR amplification on the linker-connected product using a first primer specifically bound to the first linker and a second primer specifically bound to the second linker to obtain an amplified product, the first primer or the second primer having a first affinity label; capturing a single strand having the first affinity label in the amplified product using a solid phase carrier; performing single primer linear amplification using a third primer with the captured single strand as a template; performing exponential amplification using the third primer and the first primer, with the linearly amplified product as the template, to obtain a product containing the target regions.Type: ApplicationFiled: December 9, 2015Publication date: June 14, 2018Applicants: BGI SHENZHEN, BGI SHENZHEN CO., LIMITEDInventors: Jing Guo, Rongrong GUO, Meiyan LI, Chunyu GENG, Hui JIANG
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Publication number: 20180080092Abstract: Disclosed are a one-stop treatment method for breaking a nucleic acid by means of a transposase, and a reagent. The method of the present invention comprises the following steps: conducting random breaking of a nucleic acid by using a transposase-embedded complex, the transposase-embedded complex comprising a transposase and a first adaptor comprising a transposase identification sequence; adding a first reagent to conduct treatment, so as to break an absorption effect of the transposase to a target sequence of the nucleic acid; adding a second reagent to conduct treatment, so as to weaken the influence of the first reagent on a follow-up enzyme-catalyzed reaction; and conducting a PCR reaction by using a product generated after the second reagent treatment as a template component, so as to obtain a PCR product of a broken nucleic acid segment whose two ends are connected to adaptors.Type: ApplicationFiled: October 14, 2014Publication date: March 22, 2018Applicant: BGI SHENZHEN CO., LIMITEDInventors: Chunyu GENG, Rongrong GUO, Ruoying CHEN, Yingxin ZHANG, Andrei ALEXEEV, Hui JIANG, Wenwei ZHANG
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Publication number: 20180044668Abstract: The present invention provides a novel method for ligating an adapter to a target polynucleotide and methods of generating a library of mate-pair polynucleotide constructs that employ such a ligation method. Libraries and arrays comprising mate-pair polynucleotide constructs, and methods of sequencing libraries and arrays comprising mate-pair polynucleotide constructs, are also provided.Type: ApplicationFiled: October 13, 2015Publication date: February 15, 2018Applicant: BGI SHENZHEN CO., LIMITEDInventors: Yuan JIANG, Radoje DRMANAC, Evan HUROWITZ, Andrei ALEXEEV, Xia ZHAO, Jie RUAN
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Patent number: 9890375Abstract: Provided are an isolated oligonucleotide and a use thereof in nucleic acid sequencing, wherein the isolated oligonucleotide comprises a first strand, wherein the 5?-end nucleotide of the first strand has a phosphate group, and the 3?-end nucleotide of the first strand is a dideoxynucleotide, and a second strand, wherein the 5?-end nucleotide of the second strand does not have a phosphate group, and the 3?-end nucleotide of the second strand is a dideoxynucleotide, wherein the first strand is longer than the second strand in length, and a double-stranded structure is formed between the first strand and the second strand.Type: GrantFiled: September 12, 2014Date of Patent: February 13, 2018Assignee: BGI SHENZHEN CO., LIMITEDInventors: Chunyu Geng, Dennis G. Ballinger, Yanyan Zhang, Shujin Fu, Lingyu He, Wenwei Zhang, Hui Jiang
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Publication number: 20170305970Abstract: Disclosed are a conotoxin polypeptide ?-CPTx-bt101, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 18 amino acids, has a molecular weight of 1872.72 daltons, and has the full sequence KCCTMSVCQPPPVCTCCA (SEQ. ID NO. 1).Type: ApplicationFiled: September 30, 2014Publication date: October 26, 2017Applicant: BGI SHENZHEN CO., LIMITEDInventors: Zhilong Lin, Bo Wen, Ting Tong, Jie Liu, Chaoqin Du, Fen Mo, Chao Peng, Qiong Shi
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Publication number: 20170298104Abstract: Disclosed are a conotoxin polypeptide ?-CPTx-bt103, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 29 amino acids, has a molecular weight of 3141.43 daltons, and has the full sequence RTNCGETCLKDEQCVGACQICVPSQLKCL (SEQ ID NO. 1).Type: ApplicationFiled: September 30, 2014Publication date: October 19, 2017Applicant: BGI SHENZHEN CO., LIMITEDInventors: Zhilong Lin, Bo Wen, Ting Tong, Jie Liu, Chaoqin Du, Fen Mo, Chao Peng, Qiong Shi
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Publication number: 20170292153Abstract: Provided are a method for breaking a nucleic acid and adding an adaptor by means of a transposase, and a reagent.Type: ApplicationFiled: October 14, 2014Publication date: October 12, 2017Applicant: BGI SHENZHEN CO., LIMITEDInventors: Chunyu GENG, Ruoying CHEN, Rongrong GUO, Andrei ALEXEEV, Yingxin ZHANG, Hui JIANG, Wenwei ZHANG
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Publication number: 20170234852Abstract: The present invention relates to a disease-specific metabolite profile, and particularly to a biomarker composition obtained by screening from urine-specific metabolite profiles of coronary heart disease subjects. The present invention also relates to a use of the biomarker compositions in risk assessment, diagnosis, early diagnosis, or pathological staging of coronary heart disease, and to a method for risk assessment, diagnosis, early diagnosis, or pathological staging of coronary heart disease. The biomarker composition as provided by the present invention can be used for early diagnosis of coronary heart disease and has high sensitivity, good specificity and good application prospects.Type: ApplicationFiled: September 30, 2014Publication date: August 17, 2017Applicants: BGI SHENZHEN CO., LIMITED, BGI SHENZHENInventors: Qiang Feng, Zhipeng Liu, Nan Meng, Jun Wang
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Patent number: 9359642Abstract: Provided are a method of constructing a nucleic acid library, a method of determining a nucleic acid sequence of a nucleic acid sample, and a kit thereof. The method of constructing the nucleic acid library includes the following steps: subjecting a nucleic acid sample to a DOP-PCR amplification, to obtain a first PCR amplification product; subjecting the first PCR amplification product to a second PCR amplification using a DOP-Amp primer, to obtain a second PCR amplification product; and subjecting the second PCR amplification product to an adaptor-ligation PCR, to obtain a third PCR amplification product, wherein the third PCR amplification product constitutes the nucleic acid library.Type: GrantFiled: October 16, 2012Date of Patent: June 7, 2016Assignees: BGI SHENZHEN CO., LIMITED, BGI SHENZHENInventors: Xuyang Yin, Chunlei Zhang, Hui Jiang, Xiuqing Zhang, Shengpei Chen
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Patent number: 9238840Abstract: A method for analyzing a genome of a single cell is provided, and a kit is also provided. The method for analyzing the genome of the single cell may comprise separating and lysing the single cell to obtain a whole-genome DNA of the cell; subjecting the whole-genome DNA to a whole-genome amplification to obtain a whole-genome amplification product; performing a PCR amplification using the whole-genome amplification product as template and using housekeeping-gene-specific primers to detect the housekeeping gene of the whole-genome amplification product; and determining whether the whole genome amplification product meets a requirement for sequencing based on the detection result, wherein a uniform distribution of the amplification product in each chromosome is an indication of the amplification product meeting the requirement for sequencing.Type: GrantFiled: December 29, 2011Date of Patent: January 19, 2016Assignee: BGI-Shenzhen Co., LimitedInventors: Xuyang Yin, Li Bao, Xun Xu, Hanjie Wu, Xiaoyu Liu, Xiuqing Zhang, Huanming Yang
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Publication number: 20150376697Abstract: A method and system to determine biomarkers related to abnormal condition in a subject are provided, comprising:sequencing nucleic acid samples from a first and a second subject in order to obtain multiple sequences respectively consisting of the first and the second sequencing results, wherein the first subject is in the abnormal condition; and the second subject is not in the abnormal condition; and the nucleic acid samples from the first and the second subject are both isolated from the samples of the same type; and the first and the second subject belong to the same species; and determining the biomarkers related to the abnormal condition in the subject based on the difference between the first and the second sequencing results.Type: ApplicationFiled: August 22, 2012Publication date: December 31, 2015Applicants: BGI-Shenzhen, BGI Shenzhen, Co., LimitedInventors: Shenghui Li, Junjie Qin, Jianfeng Zhu, Dongya Zhang, Zhuye Jie, Jun Wang, Jian Wang, Huanming Yang