Patents Assigned to Bio-Rad Laboratories, Inc.
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Publication number: 20230070234Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.Type: ApplicationFiled: November 14, 2022Publication date: March 9, 2023Applicant: Bio-Rad Laboratories, Inc.Inventors: David P. STUMBO, George CARMAN, Steve HOBBS, Anthony J. MAKAREWICZ, JR., Dmitri SIMONIAN, David GLADE, Joshua OEN, Denis PRISTINSKI, John DZENITIS
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Patent number: 11597920Abstract: The subject application pertains to compositions and methods for enhancing reverse transcriptase (RT) activity and/or reducing the inhibition of RT by inhibitors, such as formalin, tannic acid and/or heparin. In some embodiments, RT inhibition is reduced by the addition of potassium glutamate, histidine hydrochloride monohydrate, poloxamer 188, or any combination thereof to a reaction mixture comprising a polymerase. In other embodiments, RT is enhanced through the addition of a polyvinyl sulfonic acid sodium salt (PVSA) to a reaction mixture. The subject application also provides oligonucleotide primers for use in the reverse transcription of target sequences and its enhancement. These primers have high GC content or low GC content. Methods of using a RT inhibition reducer or a RT enhancer in a composition with an RNA template and RT improves RT yield, RT sensitivity, or RT tolerance to various chemicals are also provided.Type: GrantFiled: June 25, 2020Date of Patent: March 7, 2023Assignee: BIO-RAD LABORATORIES, INC.Inventors: Xiangdong Meng, Xiaosong Gong, Cindy C T Wan
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Patent number: 11596908Abstract: The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.Type: GrantFiled: August 20, 2018Date of Patent: March 7, 2023Assignee: Bio-Rad Laboratories, Inc.Inventors: Darren Roy Link, John Brian Hutchison, Michael L. Samuels, Michael Weiner
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Patent number: 11592658Abstract: The present invention relates in general to microscopy systems. In particular, the present invention relates to microscopes rendering digital images of samples, with the capability to digitally control the focus of the microscope system, and the software used to control the operation of the digital microscope system. Further, the present invention relates to a microscope structure that allows for compact and multi-functional use of a microscope, providing for light shielding and control with samples that require specific light wavelength characteristics, such as fluorescence, for detection and imaging. The microscope is adjustable, with a structure that can move along range(s) of motion and degree(s) of freedom to allow for ease of access to samples, shielding of samples, and manipulation of a display apparatus.Type: GrantFiled: October 17, 2017Date of Patent: February 28, 2023Assignee: Bio-Rad Laboratories, Inc.Inventors: Frank Hsiung, Kelly Kroeger, Robert Archer, Veronika Kortisova, Teresa Rubio, Amber Iraeta
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Patent number: 11590486Abstract: A method for manufacturing a mixed-mode chromatography medium is provided. The method can include, for example, oxidizing diol groups on diol-functionalized solid particles having pores of a median diameter of 0.5 micron or greater with substantially no pores of 0.1 micron or less in diameter and having a diol density of from about 200 to about 300 ?mol/mL to aldehyde groups, thereby converting said diol-functionalized solid particles to aldehyde-functionalized solid particles; and coupling amine-functionalized ligands to said aldehyde-functionalized solid particles, said amine-functionalized ligands comprising an amine-substituted hydrophobic group joined to an acid moiety selected from the group consisting of a carboxyl group and a sulfo group.Type: GrantFiled: August 31, 2020Date of Patent: February 28, 2023Assignee: Bio-Rad Laboratories, Inc.Inventors: Jiali Liao, Lee Olech, Hong Chen, Xuemei He, Russell Frost
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Publication number: 20230057343Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.Type: ApplicationFiled: June 30, 2022Publication date: February 23, 2023Applicant: Bio-Rad Laboratories, Inc.Inventors: Duc DO, Claudia LITTERST, Dianna MAAR
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Patent number: 11578322Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.Type: GrantFiled: May 5, 2020Date of Patent: February 14, 2023Assignee: Bio-Rad Laboratories, Inc.Inventors: Kalyan Handique, Austin Payne, Sida Wang, Patrick Michael Tegels, William T. Koederitz, Daniel Genord, Grey Parker, Brian Boniface, Katlyn Curtin Mehne, Alec William Hitchiner
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Patent number: 11579155Abstract: Methods, apparatuses, and systems are disclosed for analyzing quality control (QC) strategies that are applied to testing processes an analyte in order to meet an acceptable level of probability of patient harm that could result from incorrect test results. The measure of patient harm takes into account severity of patient harm, as well as its occurrence. Methods include calculating, based on the parameters of the QC strategies and the test apparatus, an expected number of incorrect final results E(Nuf) due to a test system failure. The value of E(Nuf) can be used as part of a calculation of a predicted level of probability patient harm. The ratio of the acceptable level of probability of patient harm to the predicted level of probability patient harm can determine the adequacy of the QC strategies.Type: GrantFiled: December 20, 2018Date of Patent: February 14, 2023Assignee: Bio-Rad Laboratories, Inc.Inventors: John Christopher Yundt-Pacheco, Lakshmi Kuchipudi, Curtis Alan Parvin
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Patent number: 11547995Abstract: The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.Type: GrantFiled: October 12, 2021Date of Patent: January 10, 2023Assignee: Bio-Rad Laboratories, Inc.Inventors: Kalyan Handique, Ronald Lebofsky
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Patent number: 11553116Abstract: An electronic component assembly having thermal pads with thermal vias coupling an image sensor and a camera board fab is provided for heat dissipation. The electronic component assembly can include: a circuit board having at least one thermal pad disposed on a top surface of the circuit board; and an image sensor disposed on the top surface of the circuit board, having at least one conductive pad disposed at at least one corner of the image sensor. The at least one thermal pad is coupled to the at least one conductive pad of the image sensor and the at least one thermal pad is formed with a plurality of first thermal vias penetrating the thermal pad and the circuit board for transfer of heat of the image sensor.Type: GrantFiled: December 1, 2020Date of Patent: January 10, 2023Assignee: BIO-RAD LABORATORIES, INC.Inventors: Stephen Swihart, Li Lu, Wade Jameson, Evan Thrush, Michael Griffin, Evelio Perez
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Patent number: 11549731Abstract: A heat pump that includes a thermoelectric device(s) and a heat sink having a raised portion with a top surface for thermally coupling with a planar face of the thermoelectric device(s). The raised portion of the heat sink includes an outer periphery and a raised central region surrounded by a void region to provide more uniform thermal conductivity when clamped within an assembly. The raised central region is shaped in an any shape corresponding to a shape of uneven thermal conductivity due to clamping pressure applied to the heat sink. The void region can be substantially contiguous and entirely circumscribe the central raised region. The device can optionally include discrete supports formed of a less thermally-conductive material within the void region. The supports can be elastomeric, such as O-rings, and disposed within pockets defined within the void region.Type: GrantFiled: January 2, 2020Date of Patent: January 10, 2023Assignee: Bio-Rad Laboratories, Inc.Inventors: Amir Sadri, Nenad Kircanski, Thanh-Vi Tran, Carl Marlowe, Brian David Wilson
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Patent number: 11542541Abstract: Methods, systems, and devices for sampling/isolating material from cells. An exemplary system may comprise a chip including an electrode array of sampling electrodes arranged along a surface of the chip. A cell-receiving area may be located adjacent the surface of the chip. The system also may comprise a tag array of tags supported by the chip and aligned with the electrode array. Each tag of the tag array may include an identifier that is unique to the tag within the tag array. Each tag may be configured to bind nucleic acids, or a capturing agent distinct from the tag may be aligned with each sampling electrode of the electrode array to capture a protein or other analyte of interest. The system further may comprise a control circuit configured to apply an individually controllable voltage to each sampling electrode of the electrode array and measure an electrical property of the sampling electrode.Type: GrantFiled: November 25, 2020Date of Patent: January 3, 2023Assignee: Bio-Rad Laboratories, Inc.Inventors: Arkadiusz Bibillo, Pranav Patel, Christopher Reggiardo, Jonathan Petersen
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Patent number: 11534727Abstract: The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.Type: GrantFiled: October 11, 2016Date of Patent: December 27, 2022Assignee: Bio-Rad Laboratories, Inc.Inventors: Darren Link, John Brian Hutchison, Michael Samuels, Michael Weiner
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Patent number: 11525813Abstract: A container assembly for use with a high-pressure liquid chromatography (HPLC) instrument is disclosed, in which the container assembly, when coupled to a source of pressurized gas, provides fluid medium to the HPLC instrument at positive pressure. The container assembly has an external exterior container shell, an internal fluid container for holding fluid medium, an interstitial volume between the external exterior container shell and the internal fluid container, a port for fluidly connecting the volume to a pressurized gas source, and a port for fluidly connecting the internal fluid container to the HPLC instrument. As a pressurized gas in the interstitial volume increases, fluid medium flows out of the port connected to the internal fluid bag and container assembly at a positive pressure. A system incorporating the container assembly, and method of use of the same, are also disclosed.Type: GrantFiled: January 31, 2018Date of Patent: December 13, 2022Assignee: Bio-Rad Laboratories, Inc.Inventors: Nathan Wrench, William Mainwaring-Burton, Chris Butcher, Nick Harrison
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Patent number: 11511242Abstract: The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.Type: GrantFiled: October 25, 2021Date of Patent: November 29, 2022Assignee: Bio-Rad Laboratories, Inc.Inventors: Darren Roy Link, John Brian Hutchison, Michael L. Samuels, Michael Weiner
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Patent number: 11504719Abstract: A system and method for receiving and delivering a fluid, the system comprising: a body configured to interface with an opening of a reservoir and defining: a protrusion defining a set position of the body relative to the reservoir; a wall extending from the protrusion; a receiving surface coupled to the wall and sloping from an apex to a nadir along a first direction, the receiving surface comprising a vent; and an outlet positioned closer to the nadir than the apex of the receiving surface and displaced from the vent, the outlet comprising an extension from the body, the extension configured to contact an interior wall of the reservoir, wherein the body comprises: a bubble-mitigating operation mode in which the receiving surface receives and transmits the fluid along the receiving surface, and a fluid-transmitting operation mode in which the body directs the fluid along the interior wall of the reservoir.Type: GrantFiled: March 12, 2020Date of Patent: November 22, 2022Assignee: Bio-Rad Laboratories, Inc.Inventors: Kalyan Handique, Austin Payne
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Patent number: 11504714Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.Type: GrantFiled: February 13, 2020Date of Patent: November 22, 2022Assignee: Bio-Rad Laboratories, Inc.Inventors: Kalyan Handique, Vishal Sharma, Priyadarshini Gogoi, William Chow, Austin Payne, Kyle Gleason, Brian Boniface, John Connolly, Sam Tuck
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Publication number: 20220362764Abstract: The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g.Type: ApplicationFiled: May 20, 2022Publication date: November 17, 2022Applicant: Bio-Rad Laboratories, Inc.Inventors: Benjamin J. HINDSON, Serge SAXONOV, Phillip BELGRADER, Kevin D. NESS, Michael Y. LUCERO, Billy W. COLSTON, JR., Shawn Paul HODGES, Nicholas J. HEREDIA, Jeffrey Clark MELLEN, Camille Bodley TROUP, Paul WYATT
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Patent number: 11499181Abstract: Method of haplotype analysis. In an exemplary method, an aqueous phase containing nucleic acid may be partitioned into a plurality of discrete volumes. At least one allele sequence may be amplified in the volumes from each of a first polymorphic locus and a second polymorphic locus that exhibit sequence variation in the nucleic acid. At least one measure of co-amplification of allele sequences from both loci in the same volumes may be determined. A haplotype of the first and second loci may be selected based on the at least one measure of co-amplification.Type: GrantFiled: December 28, 2018Date of Patent: November 15, 2022Assignee: Bio-Rad Laboratories, Inc.Inventors: John F. Regan, Serge Saxonov, Michael Y. Lucero, Benjamin J. Hindson, Phillip Belgrader, Simant Dube, Austin P. So, Jeffrey C. Mellen, Nicholas J. Heredia, Kevin D. Ness, Billy W. Colston, Jr.
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Patent number: 11499183Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.Type: GrantFiled: June 28, 2018Date of Patent: November 15, 2022Assignee: Bio-Rad Laboratories, Inc.Inventors: David P. Stumbo, George Carman, Steve Hobbs, Anthony J. Makarewicz, Jr., Dmitri Simonian, David Glade, Joshua Oen, Denis Pristinski, John Dzenitis