Abstract: A method of analyzing a sample possibly containing an HIV virus, including a) extracting viral RNA in a biological sample that possibly contains an HIV virus; b) reverse transcription of the RNA obtained of (a) and amplification with a first pair of primers to obtain an amplified product of reverse transcription including all or part of at least two successive genes of a genome of an HIV virus; and one or both of c) and d): c) sequencing the amplified product of (b) to establish a genotype of HIV virus present in the sample and identify mutations that may be present in the amplified product; d1) amplifying the product of (b) with a second pair of primers complementary to the first pair of (b) and capable of generating an amplification product that can be inserted by homologous recombination into a retroviral vector that is defective in a region corresponding to the amplified product; d2) homologously recombining the product of (d1) with the defective vector; d3) functionally analyzing the viral proteins coded
Type:
Application
Filed:
March 7, 2005
Publication date:
December 28, 2006
Applicant:
Bioalliance Pharma, a corporation of France
Inventors:
Sophie Lebel-Binay, Elisabeth Dam, Luc Boblet, Dominique Costantini
Abstract: A pharmaceutical composition for the treatment, prevention or diagnosis of a tumoral pathology comprising an active agent which stabilizes an actin network of a cellular cytoskeleton.
Type:
Application
Filed:
December 18, 2003
Publication date:
September 30, 2004
Applicants:
Bioalliance Pharma, a corporation of France, Ecole Normale Superieure de Cachan, a corporation of France, Institut Gustave Roussy - IGR, a corporation of France, Centre National de la Recherche Scientifique - CNRS, a corporation of France
Inventors:
Christian Auclair, Valerie Amsellem, Martial Hervy, Frederic Subra