Patents Assigned to Boehringer Mannheim Corporation
  • Patent number: 5753485
    Abstract: A purified enzyme mixture useful for isolating cells or cell clusters from tissue is disclosed. The mixture includes at least two collagenase enzymes, at least two other proteases, and additional non-protease components. The mixture is purified by removing at least some of the non-protease components. The purified mixture may then be used to isolate cells or cell clusters from tissue. Also disclosed are the essential components of the purified enzyme mixture as well as preferred ranges and ratios of these essential components for isolating cells or cell clusters from tissue. Finally, a dissociation system is disclosed that can be used with the purified enzyme mixture to dissociate tissue and recover cells or cell clusters.
    Type: Grant
    Filed: January 24, 1996
    Date of Patent: May 19, 1998
    Assignee: Boehringer Mannheim Corporation
    Inventors: Francis E. Dwulet, Bernice B. Ellis, John F. Gill, Linda B. Jacobsen, Marilyn E. Smith, David G. Waters
  • Patent number: 5724066
    Abstract: A system and method provide for distinguishing among characters of a character set. The characters are represented by a data stream of pixel information to a display port. A subset of the pixel information in the data stream contains sufficient information for unique identification of each character within the character set. Examination of the data within the subset of pixels permits identification of respective characters within the set.
    Type: Grant
    Filed: April 18, 1995
    Date of Patent: March 3, 1998
    Assignee: Boehringer Mannheim Corporation
    Inventors: Richard O. Carmack, Howard Clarence Freeman, Charles V. Green, David S. Herron, Darren Carl Martin, G. Scott Rulong, Mark J. Simmons, Karl Szabo, Raymond E. Szkotak, Li-Wun Chen Tam, Robert C. Uleski, Frank W. Wagle, Stephen T. West
  • Patent number: 5719063
    Abstract: Disclosed is a method of assaying and apparatus for assaying, in assay apparatus, for an analyte in a sample by specifically binding the analyte to a binding molecule having a detectable tag to form a complex, the presence of which can be determined quantitatively by measuring the amount of tag. In one embodiment, the sample is contacted in that apparatus with a complex of binding molecules so as to effect the specific binding. The complex has at least two differently tagged different binding molecules in a predetermined ratio, at least a first of which tagged binding molecules being that which will specifically bind to the analyte. The second tagged binding molecule is one which will not specifically bind to the analyte or to other moieties in the sample. The relative non-specific binding characteristics of those binding molecules with respect to said apparatus are in a known or predetermined relationship.
    Type: Grant
    Filed: April 4, 1994
    Date of Patent: February 17, 1998
    Assignee: Boehringer Mannheim Corporation
    Inventor: Myron J. Block
  • Patent number: 5710622
    Abstract: A method of immunizing the operation of an instrument which determines a characteristic of a biological fluid or a control against noise. The instrument includes a surface upon which a user deposits a sample of biological fluid or control the characteristic of which is to be determined. The instrument irradiates the sample and detects radiation from the sample to determine the characteristic.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: January 20, 1998
    Assignee: Boehringer Mannheim Corporation
    Inventors: Gary T. Neel, James R. Parker, Rick L. Collins, David E. Storvick, Charles L. Thomeczek, Jr., William J. Murphy, George R. Lennert, Morris J. Young, Daniel L. Kennedy
  • Patent number: 5707820
    Abstract: A new reagent and methods for measuring the concentration of (or detecting the presence of) an analyte in a sample. The reagent includes a phenazine-containing compound and an enzyme. The phenazine-containing compound must be of sufficient type to form a semiquinoid (the color indicator) by reaction involving the enzyme, analyte, and phenazine-containing compound. Importantly, the phenazine-containing compound must be in sufficient amount to correlate the concentration of semiquinoid to the concentration of analyte in the sample or to detect the presence of the analyte in the sample. The reagent may further include a buffer and a surfactant. The reagent may be incorporated into a film and may be provided in kit form.
    Type: Grant
    Filed: February 7, 1996
    Date of Patent: January 13, 1998
    Assignee: Boehringer Mannheim Corporation
    Inventors: Christopher D. Wilsey, Helmut Freitag
  • Patent number: 5686659
    Abstract: A method of determining whether a sample or a control has been applied to an instrument for measuring coagulation of a fluid by optically detecting when the fluid that has been applied to the instrument has passed a first and a second points, determining the flow time for the fluid between the two points, establishing a time threshold, and the flow time to the threshold to determining if the fluid is a sample or a control.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: November 11, 1997
    Assignee: Boehringer Mannheim Corporation
    Inventors: Gary T. Neel, James R. Parker, Rick L. Collins, David E. Storvick, Charles L. Thomeczek, Jr., William J. Murphy, George R. Lennert, Morris J. Young, Daniel L. Kennedy
  • Patent number: 5652755
    Abstract: Monitor (11) includes a microcontroller (51) programmed in a first mode of operation to drive various loads (59), (69) which operate to perform tests on patient samples. The results of the tests are sensed by sensors (61), (75) and digitally stored in storage (67) for display on display (21). The microcontroller (51) is programmed in a second mode of operation to transmit the stored displayed information to a printer (13) over the line (23) transmitting power to the monitor (11) by selectively routing current through the load (69). A data transmission interface (15) includes circuitry (37) (39) (43) for sensing the changing current provided to the monitor (11) for decoding the information to be printed and for sending print information to printer (13). Error checking is performed by microprocessor (39) which inhibits all printing if any errors occur in the received message.
    Type: Grant
    Filed: February 3, 1994
    Date of Patent: July 29, 1997
    Assignee: Boehringer Mannheim Corporation
    Inventor: Brian Carey
  • Patent number: 5639631
    Abstract: The invention is a stable reagent used in assay systems that form a colored ferric ion complex, such as Prussian Blue. Such reagents are useful for the detection or measurement of an analyte from a fluid sample. Surprisingly, it has been found that the inclusion of certain ferric ion chelating agents, such as 3-sulfobenzoic acid, will inhibit formation of the blank reaction in the reagent.
    Type: Grant
    Filed: June 22, 1995
    Date of Patent: June 17, 1997
    Assignee: Boehringer Mannheim Corporation
    Inventors: Chi-Neng Arthur Han, Kenneth J. Dean, Walter W. Jernigan, William R. Carr
  • Patent number: 5639668
    Abstract: An optical system which may be a component of apparatus for assaying a fluid sample with radiation is capable of exciting fluorescence in fluorescent material and includes a totally internally reflecting, unitary elongated substrate transmissive to both the excitation radiation and to the fluorescence. The fluorescent material includes at least a moiety of an antibody-antigen complex that includes a tag that will provide the fluorescence when excited by an evanescent wave generated by the excitation radiation. The substrate includes an elongated fiber and an integral lens formed to guide the optical radiation into the fiber within the bounds of a critical angle to assure total internal reflectance. A hollow elongated enclosure is disposed concentrically about and spaced from the fiber so as to provide an interspace of capillary dimensions.
    Type: Grant
    Filed: September 14, 1995
    Date of Patent: June 17, 1997
    Assignee: Boehringer Mannheim Corporation
    Inventors: Thomas Gary Neel, James E. Lyst, Jr.
  • Patent number: 5627075
    Abstract: The inclusion of succinic acid or a salt thereof to stabilize compositions that include glucose oxidase and/or ferricyanide. Typically, the succinic acid or succinic acid salt is included in a diagnostic reagent utilized in test strips or test solutions useful for the analysis of glucose from a fluid sample.
    Type: Grant
    Filed: March 20, 1996
    Date of Patent: May 6, 1997
    Assignee: Boehringer Mannheim Corporation
    Inventor: Joseph E. Bateson
  • Patent number: 5606164
    Abstract: A method and apparatus for measuring the concentration of an analyte present in a biological fluid is disclosed. The method includes the steps of applying NIR radiation to calibration samples to produce calibration data, analyzing the calibration data to identify and remove outliers, constructing a calibration model, collecting and analyzing unknown samples to identify and remove outliers, and predicting analyte concentration of non-outliers from the calibration model. Analysis of the calibration data includes data pretreatment, data decomposition to remove redundant data, and identification and removal of outliers using generalized distances. The calibration model may utilize principal component regression, partial least squares, multiple linear regression, or artificial neural networks, and reduction using principal component analysis or partial least squares scores.
    Type: Grant
    Filed: January 16, 1996
    Date of Patent: February 25, 1997
    Assignee: Boehringer Mannheim Corporation
    Inventors: John F. Price, James R. Long
  • Patent number: 5594906
    Abstract: A serial data interface includes a receive data terminal, a received serial data out terminal and an operating power supply terminal. A zero-power receive detector for the serial data interface includes a source for furnishing operating power to the interface. The supply of power from the source is controlled through a microcontroller for switching power to the interface. The microcontroller is coupled between the source and the operating power supply terminal of the interface. A light source portion of an optical isolator is coupled to the receive data terminal, and a light activated switch portion of the optical isolator is coupled to the microcontroller.
    Type: Grant
    Filed: July 7, 1994
    Date of Patent: January 14, 1997
    Assignee: Boehringer Mannheim Corporation
    Inventors: John S. Holmes, II, Joe E. Anderson
  • Patent number: 5589326
    Abstract: A new group of Os(II) and Os(III) compounds useful as redox mediators in electrochemical biosensors. These compounds have 1) low oxidation potential, 2) fast reaction kinetics between the electroactive center of an enzyme and the compound, 3) slow oxidation of osmium by oxygen, and 4) excellent solubility in aqueous medium. These mediators are particularly useful as a component of a reagent used in an electrochemical biosensor, wherein the biosensor is useful for measuring analytes from a biological fluid, such as blood.
    Type: Grant
    Filed: December 30, 1993
    Date of Patent: December 31, 1996
    Assignee: Boehringer Mannheim Corporation
    Inventors: Zhi D. Deng, Gerald F. Sigler, Nigel A. Surridge, Christopher D. Wilsey, Robert J. McEnroe, Walter W. Jernigan, Rebecca W. Muddiman
  • Patent number: 5583006
    Abstract: A stable reagent for assaying an analyte from a fluid sample which includes an enzyme, a mediator, a tetrazolium salt and an oxidizing agent selected from the group consisting of sodium chlorate, 2,5-dimethylhexane-2,5-dihydroperoxide, benzoyl peroxide, t-butyl peroxide, sodium iodate, N-ethylmaleimide, t-butylperoxyacetate, nickel acetylacetonate, stannic chloride, rhodium (III) trichloride hydrate, and t-butylperbenzoate is described. Inclusion of an oxidizing agent from the group specified above stabilizes the reagent, thereby preventing a high blank reaction because a high blank introduces error into the assay of an analyte. The reagent may be provided in solution form by adding water, or may be included in a film by adding a film-forming agent. The reagent may be utilized to perform the assay of an analyte from a fluid sample.
    Type: Grant
    Filed: May 4, 1995
    Date of Patent: December 10, 1996
    Assignee: Boehringer Mannheim Corporation
    Inventors: Diana F. Storhoff, David Tabb
  • Patent number: 5543299
    Abstract: The invention relates to polymeric compositions useful in analyte determination. The compositions contain a polymer, a reagent system for analyte determination, and an extender. The last component alleviates tackiness in the composition, and thus reduces damage in preparation of test apparatus. Mica is the particularly preferred extender.
    Type: Grant
    Filed: January 5, 1995
    Date of Patent: August 6, 1996
    Assignee: Boehringer Mannheim Corporation
    Inventors: Eric Diebold, Myron Rapkin, Abol Azhar, Arthur Usmani
  • Patent number: 5538870
    Abstract: Methods useful in purifying nucleic acids from whole cell samples are described. This is accomplished by adding one or both of a non-phenyl group containing non-ionic detergent or a cross-linked, polycarboxylic acid. Kits which can be used in the methods are also described.
    Type: Grant
    Filed: September 21, 1994
    Date of Patent: July 23, 1996
    Assignee: Boehringer Mannheim Corporation
    Inventors: Lisa S. Noeth, Mary Dasovich-Moody, Melissa R. Winget
  • Patent number: 5525474
    Abstract: Novel derivatives of procainamide and N-acetylprocainamide (NAPA) are disclosed having the following formula: ##STR1## wherein: X=hydrogen or acetyl;n=1 to p where p=MW of Z/1000;Z=a poly(amino acid) or polysaccharide; andR3=a bond or ##STR2## wherein R2=an alkyl, cycloalkyl or aryl group having 2 to 10 carbon atoms. The derivatives include maleimide conjugates of proteins or poly(amino acids), enzymes, enzyme donor polypeptides and labeling substances. Novel activated hapten intermediates useful in the preparation of the conjugates and methods for synthesis of the hapten intermediates and derivatives are also disclosed.
    Type: Grant
    Filed: January 31, 1994
    Date of Patent: June 11, 1996
    Assignee: Boehringer Mannheim Corporation
    Inventors: Gerald F. Sigler, Charles F. Walter, Todd Glancy, Erasmus Huber, Frank E. Klein
  • Patent number: 5526111
    Abstract: A method for calculating a coagulation characteristic of a sample of blood, a blood fraction, or a control comprises generating an output signal indicative of coagulation of the sample, sampling the output signal at a sampling rate for a predetermined time to provide a plurality of sampled signal values, storing the sampled signal values, rectifying the stored sampled signal values to provide a plurality of envelope values, and storing the envelope values. The stored envelope values are examined in reverse time order to determine a slope of the envelope at each of the stored envelope values. All relative maxima in the stored envelope values are identified. A range of stored envelope values corresponding to each relative maximum is determined. The identified relative maxima are compared to locate an absolute maximum in the stored envelope values.
    Type: Grant
    Filed: August 31, 1993
    Date of Patent: June 11, 1996
    Assignee: Boehringer Mannheim Corporation
    Inventors: Rick L. Collins, Martin T. Gerber, Zindel H. Heller, James L. Maxwell, Edna C. Probst, Loy M. Vail, Stefan Weinert, Morris J. Young
  • Patent number: 5522255
    Abstract: An instrument and a method for determining a coagulation characteristic of blood, a blood fraction or a control comprise a radiation-reflective surface, a first source for irradiating the surface, and a first detector for detecting radiation reflected from the surface. A cuvette holds a sample of the blood, blood fraction or control the coagulation characteristic of which is to be determined. The cuvette has two opposed walls substantially transparent to the source radiation and reflected radiation. The first source and first detector are disposed adjacent a first one of the two opposed walls and the radiation reflective surface is disposed adjacent a second of the two opposed walls.
    Type: Grant
    Filed: August 31, 1993
    Date of Patent: June 4, 1996
    Assignee: Boehringer Mannheim Corporation
    Inventors: Gary T. Neel, James R. Parker, Rick L. Collins, David E. Storvick, Charles L. Thomeczek, Jr., William J. Murphy, George R. Lennert, Morris J. Young, Daniel L. Kennedy
  • Patent number: 5514560
    Abstract: Galactopyranoside derivative compounds for use as substrates for hydrolysis by enzymes with .beta.-galactosidase activity are provided. The concentration of the reaction products can be measured by spectroscopy. The compounds find particular use in diagnostic assays for detection of analyte.
    Type: Grant
    Filed: May 30, 1995
    Date of Patent: May 7, 1996
    Assignee: Boehringer Mannheim Corporation
    Inventors: Wayne B. Manning, Pyare Khanna, Glenda Choate