Patents Assigned to Carnegie Institution of Washington
  • Patent number: 10466233
    Abstract: A nanosensor for detecting and quantifying lactate in different types of samples, such as tissues, intra-cellular and subcellular compartments, with high spatial and temporal resolution is disclosed. Methods comprising use of the nanosensor for quantifying the activity of lactate transporters, rates of cellular lactate production and cellular lactate consumption, and rate of mitochondrial pyruvate consumption are also disclosed. Methods for quantifying the transformation in energy metabolism that characterizes cancer cells with single-cell resolution and for detecting interference of candidate drugs with mitochondrial energetics are additionally disclosed.
    Type: Grant
    Filed: April 13, 2012
    Date of Patent: November 5, 2019
    Assignees: CENTRO DE ESTUDIOS CIENTIFICOS DE VALDIVIA, CARNEGIE INSTITUTION OF WASHINGTON
    Inventors: Luis Felipe Barros Olmedo, Alejandro San Martin, Sebastian Ceballo Charpentier, Wolf B. Frommer
  • Patent number: 10358653
    Abstract: A process is provided of introducing an RNA into a living cell to inhibit gene expression of a target gene in that cell. The process may be practiced ex vivo or in vivo. The RNA has a region with double-stranded structure. Inhibition is sequence-specific in that the nucleotide sequences of the duplex region of the RNA and of a portion of the target gene are identical. The present invention is distinguished from prior art interference in gene expression by antisense or triple-strand methods.
    Type: Grant
    Filed: January 22, 2016
    Date of Patent: July 23, 2019
    Assignees: The Carnegie Institution of Washington, The University of Massachusetts
    Inventors: Andrew Fire, Stephen Kostas, Mary Montgomery, Lisa Timmons, SiQun Xu, Hiroaki Tabara, Samuel E. Driver, Craig C. Mello
  • Patent number: 10280393
    Abstract: The present invention relates generally to an integrated system, apparatus and method that allows for the continuous culturing of microorganisms under high pressure conditions and at a wide range of temperatures. More specifically, the system is configured to be gas tight and operate under aerobic or anaerobic conditions. The system is also configured to permit periodic sampling of the incubated organisms under such conditions with minimal physical/chemical disturbance inside the reactor and minimal impacts of shear forces on the collected biomass.
    Type: Grant
    Filed: February 27, 2015
    Date of Patent: May 7, 2019
    Assignee: CARNEGIE INSTITUTION OF WASHINGTON
    Inventor: Dionysis Ioannis Foustoukos
  • Patent number: 10246721
    Abstract: A novel class of transporter protein, referred to as SWEET, GLUE or Glü, is disclosed. These transporters provide a novel system for the transportation of sugars across membranes within a cell and between the inside and outside of a cell. Such transporters are useful for understanding and altering the sugar concentration within certain organs of an organism, and within certain organelles within the cell. These transporters are also useful in protecting plants from a pathogen attack.
    Type: Grant
    Filed: November 10, 2016
    Date of Patent: April 2, 2019
    Assignee: Carnegie Institution of Washington
    Inventors: Wolf B. Frommer, Sylvie Lalonde
  • Patent number: 10179740
    Abstract: The invention relates to a new phase of silicon, Si24, and a method of making the same. Si24 has a quasi-direct band gap, with a direct gap value of 1.34 eV and an indirect gap value of 1.3 eV. The invention also relates to a compound of the formula Na4Si24 and a method of making the same. Na4Si24 may be used as a precursor to make Si24.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: January 15, 2019
    Assignee: CARNEGIE INSTITUTION OF WASHINGTON
    Inventors: Timothy A. Strobel, Duck Young Kim, Oleksandr O. Kurakevych
  • Publication number: 20170355605
    Abstract: The invention relates to a new phase of silicon, Si24, and a method of making the same. Si24 has a quasi-direct band gap, with a direct gap value of 1.34 eV and an indirect gap value of 1.3 eV. The invention also relates to a compound of the formula Na4Si24 and a method of making the same. Na4Si24 may be used as a precursor to make Si24.
    Type: Application
    Filed: June 30, 2017
    Publication date: December 14, 2017
    Applicant: CARNEGIE INSTITUTION OF WASHINGTON
    Inventors: Timothy A. STROBEL, Duck Young KIM, Oleksandr O. KURAKEVYCH
  • Patent number: 9695051
    Abstract: The invention relates to a new phase of silicon, Si24, and a method of making the same. Si24 has a quasi-direct band gap, with a direct gap value of 1.34 eV and an indirect gap value of 1.3 eV. The invention also relates to a compound of the formula Na4Si24 and a method of making the same. Na4Si24 may be used as a precursor to make Si24.
    Type: Grant
    Filed: July 8, 2014
    Date of Patent: July 4, 2017
    Assignee: CARNEGIE INSTITUTION OF WASHINGTON
    Inventors: Timothy A. Strobel, Duck Young Kim, Oleksandr O. Kurakevych
  • Patent number: 9562081
    Abstract: A novel class of transporter protein, referred to as SWEET, GLUE or Glü, is disclosed. These transporters provide a novel system for the transportation of sugars across membranes within a cell and between the inside and outside of a cell. Such transporters are useful for understanding and altering the sugar concentration within certain organs of an organism, and within certain organelles within the cell. These transporters are also useful in protecting plants from a pathogen attack.
    Type: Grant
    Filed: May 4, 2010
    Date of Patent: February 7, 2017
    Assignee: CARNEGIE INSTITUTION OF WASHINGTON
    Inventors: Wolf B. Frommer, Sylvie Lalonde
  • Publication number: 20160355835
    Abstract: The present invention relates to methods of increasing the levels of at least one sugar in developing seeds in a plant, with the methods comprising inserting an exogenous nucleic acid, which codes for at least one sugar transporter protein (SWEET protein), into a plant cell to create a transgenic plant cell, and subjecting the transgenic plant cell to conditions that promote expression of the at least one SWEET protein during seed development. The methods results in transgenic plant seeds, and transgenic plants that produce seed, where the levels of at least one sugar are increased as compared to seeds from non-transgenic plants of the same species grown under the same conditions.
    Type: Application
    Filed: March 13, 2014
    Publication date: December 8, 2016
    Applicant: CARNEGIE INSTITUTION OF WASHINGTON
    Inventor: Wolf B. Frommer
  • Publication number: 20160097088
    Abstract: Novel methods of ChIP-seq are disclosed herein. These methods of ChIP-seq employ carrier DNA to prevent loss of DNA samples. The greater DNA yields achieved by this invention permit ChIP-seq of a small number of cells, permitting epigenetic analysis of primary cells of limited quantity.
    Type: Application
    Filed: September 14, 2015
    Publication date: April 7, 2016
    Applicant: Carnegie Institution of Washington
    Inventors: Yixian ZHENG, Junling JIA, Xiaobin ZHENG
  • Patent number: 9176143
    Abstract: The disclosure relates to engineered transport proteins comprising at last one fluorescent reporter covalently bound to the transporter protein, wherein the transporter proteins comprise a structural inverted repeat motif, with the motif comprising a first and second subunit that are structural inverted repeats of one another and that are joined to one another by a polypeptide loop.
    Type: Grant
    Filed: September 18, 2012
    Date of Patent: November 3, 2015
    Assignee: Carnegie Institution of Washington
    Inventors: Wolf B. Frommer, Roberto De Michele, Cindy Ast
  • Patent number: 9102939
    Abstract: A process is provided of introducing an RNA into a living cell to inhibit gene expression of a target gene in that cell. The process may be practiced ex vivo or in vivo. The RNA has a region with double-stranded structure. Inhibition is sequence-specific in that the nucleotide sequences of the duplex region of the RNA and of a portion of the target gene are identical. The present invention is distinguished from prior art interference in gene expression by antisense or triple-strand methods.
    Type: Grant
    Filed: October 8, 2012
    Date of Patent: August 11, 2015
    Assignees: The Carnegie Institution of Washington, The University of Massachusetts
    Inventors: Andrew Fire, Stephen Kostas, Mary Montgomery, Lisa Timmons, SiQun Xu, Hiroaki Tabara, Samuel E. Driver, Craig C. Mello
  • Patent number: 9023307
    Abstract: The invention relates to single crystal diamond with high optical quality and methods of making the same. The diamond possesses an intensity ratio of the second-order Raman peak to the fluorescence background of around 5 or greater.
    Type: Grant
    Filed: May 17, 2011
    Date of Patent: May 5, 2015
    Assignee: Carnegie Institution of Washington
    Inventors: Russell J. Hemley, Yu-fei Meng, Chih-Shiue Yan, Ho-kwang Mao
  • Patent number: 9023306
    Abstract: The invention relates to a single crystal boron doped CVD diamond that has a toughness of at least about 22 MPa m1/2. The invention further relates to a method of manufacturing single crystal boron doped CVD diamond. The growth rate of the diamond can be from about 20-100 ?m/h.
    Type: Grant
    Filed: May 5, 2009
    Date of Patent: May 5, 2015
    Assignee: Carnegie Institution of Washington
    Inventors: Russell J. Hemley, Ho-Kwang Mao, Chih-Shiue Yan, Qi Liang
  • Patent number: 8995724
    Abstract: The present invention provides systems and methods to automatically analyze Landsat satellite data of forests. The present invention can easily be used to monitor any type of forest disturbance such as from selective logging, agriculture, cattle ranching, natural hazards (fire, wind events, storms), etc. The present invention provides a large-scale, high-resolution, automated remote sensing analysis of such disturbances.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: March 31, 2015
    Assignee: The Carnegie Institution of Washington
    Inventor: Gregory P. Asner
  • Publication number: 20150072375
    Abstract: A nanosensor for detecting and quantifying lactate in different types of samples, such as tissues, intra-cellular and subcellular compartments, with high spatial and temporal resolution is disclosed. Methods comprising use of the nanosensor for quantifying the activity of lactate transporters, rates of cellular lactate production and cellular lactate consumption, and rate of mitochondrial pyruvate consumption are also disclosed. Methods for quantifying the transformation in energy metabolism that characterizes cancer cells with single-cell resolution and for detecting interference of candidate drugs with mitochondrial energetics are additionally disclosed.
    Type: Application
    Filed: April 13, 2012
    Publication date: March 12, 2015
    Applicants: CARNEGIE INSTITUTION OF WASHINGTON, CENTRO DE ESTUDIOS CIENTIFICOS DE VALDIVIA
    Inventors: Luis Felipe Barros Olmedo, Alejandro San Martin, Sebastian Ceballo Charpentier, Wolf B. Frommer
  • Publication number: 20140359899
    Abstract: The present invention relates to genetically modified plant cells that have altered expression or activity of at least one sucrose efflux transporter compared to levels of expression or activity of the at least one sucrose efflux transporter in an unmodified plant cell.
    Type: Application
    Filed: December 10, 2012
    Publication date: December 4, 2014
    Applicant: Carnegie Institution of Washington
    Inventor: Wolf B. Frommer
  • Publication number: 20140323692
    Abstract: Intramolecular biosensors are disclosed, including PBP-based biosensors, comprising a ligand binding domain fused to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding ligand. At least one of the donor and fluorescent moieties may be internally fused to the biosensor such that both ends of the internally fused fluorophore are fixed. In addition, methods of improving the sensitivity of terminally fused biosensors are provided. The biosensors of the invention are useful for the detection and quantification of ligands in vivo and in culture.
    Type: Application
    Filed: September 9, 2013
    Publication date: October 30, 2014
    Applicant: Carnegie Institution of Washington
    Inventors: Wolf B. Frommer, Sakiko Okumoto, Loren Looger, Marcus Fehr
  • Patent number: 8846365
    Abstract: Phosphate biosensors are disclosed, which comprise a phosphate binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon phosphate binding. Such biosensors are useful for real time monitoring of phosphate metabolism in living cells.
    Type: Grant
    Filed: October 14, 2005
    Date of Patent: September 30, 2014
    Assignee: Carnegie Institution of Washington
    Inventors: Wolf B. Frommer, Hong Gu, Sylvie Lalonde, Arthur Grossman
  • Patent number: 8721915
    Abstract: This invention relates to partially ordered and ordered oxynitride perovskites of the general formula ABO2N that are polar insulators. A comprises one or more cations or set of cations that sit in sites derived from the A-site in the perovskite structure. B comprises one or more cations or set of cations that sit in sites derived from the B-site in the perovskite structure. C comprises oxygen, O, with optionally some nitrogen, N, and D comprises N, with optionally some O. The total valence of the cations A+B is equal to the total valence of the anions 2 C+D. Also disclosed are methods of producing such oxynitride perovskites and uses of such oxynitride perovskites.
    Type: Grant
    Filed: September 13, 2012
    Date of Patent: May 13, 2014
    Assignee: Carnegie Institution of Washington
    Inventors: Ronald Cohen, Razvan Caracas