Abstract: Disclosed is an O-phosphoserine sulfhydrylase (OPSS) mutant which has a Mycobacterium smegmatis-derived amino acid sequence corresponding to that of SEQ ID NO: 1 which is devoid of three to seven C-terminal amino acid residues. Also, a nucleic acid molecule encoding the OPSS mutant, an expression vector carrying the nucleic acid molecule, and a transformant transformed with the expression vector are disclosed. In addition, a method is provided for producing cysteine in which O-phospho-L-serine (OPS) is reacted with a sulfide in the presence of the OPSS mutant. The OPSS mutant has improved enzymatic activity and can be applied to the environmentally friendly production of L-cysteine through a simple enzymatic conversion reaction.
Type:
Grant
Filed:
October 20, 2011
Date of Patent:
September 8, 2015
Assignee:
CJ Cheildjedang Corporation
Inventors:
Soo An Shin, Jin Sook Chang, Hye Won Um, Jae Hyun Jo, Byeong Cheol Song, Kyoung Min Lee
Abstract: The present invention provides methods for the production of cysteine or derivates thereof by culturing a microorganism having reduced activity of endogenous phosphoserine phosphatase and enhanced activity of phosphoglycerate dehydrogenase and/or phosphoserine aminotransferase. The O-phosphoserine produced by such an organism can then be reacted with a sulfide in the presence of a sulfydrylase or a microorganism expressing a sulfhydrylase to produce cysteine or a derivative thereof. Microorganisms having the properties noted above are also provided herein.
Type:
Grant
Filed:
October 20, 2011
Date of Patent:
October 15, 2013
Assignee:
CJ Cheildjedang Corporation
Inventors:
Jin Sook Chang, Jae Hyun Jo, Hyun Ae Bae, Byeong Cheol Song, Sol Kim, Hye Won Kim