Abstract: A rapid, competitive enzyme linked immunosorbent assay (cELISA) for the determination of Bluetongue virus antibodies in serum is described. This method utilizes either a biotinylated monoclonal antibody to Bluetongue virus and streptavadin-enzyme in conjunction with synthetic substrate, or an enzyme-conjugated monoclonal to detect antibodies specific for Bluetongue virus.
Type:
Grant
Filed:
October 9, 1990
Date of Patent:
February 23, 1993
Assignee:
Consultants for Applied Biosciences, Inc.
Inventors:
John J. Reddington, Ginger M. Reddington