Patents Assigned to Coulter Corporation
  • Patent number: 6362003
    Abstract: A method of quality control to diagnose the cause of a malfunction of an instrument. The method uses measurements of the physical property of a sample to diagnose the cause of a malfunction of an instrument. The spatial position of a control product sample is analyzed. Alternatively, the spatial position of a statistically significant number of patient blood samples can be used. The method enables the monitoring of an instrument for problems associated with debris and noise caused by red cell lysis inefficiency; instrument reagents pump volume settings; instrument laser alignments; instrument gain settings; and flow noise caused by partial plugs, residual plugs or other flow problems. The method provides a more specific indication of the type and cause of an instrument malfunctioning than non specific flagging provided by prior art methods.
    Type: Grant
    Filed: September 14, 1998
    Date of Patent: March 26, 2002
    Assignee: Coulter Corporation
    Inventors: Carole Young, Michael Elliott, Nancy Naylor, Timothy J. Fischer
  • Patent number: 6281335
    Abstract: An anti-KC-4 humanized monoclonal antibody that comprises the variable regions of the light and heavy chains of the anti-KC-4 murine antibody, wherein the light chain has 7 amino acids and the heavy chain has 12 amino acids of the framework regions substituted with amino acid present in equivalent positions in antibodies of a species other than munne, and the constant regions of a human antibody. The antibody may be labeled and/or glycosylated, and is presented as a composition with a carrier. The anti-KC-4 monoclonal antibody is used in diagnostic kits for cancer and in in vivo methods of imaging and treating a primary or metastasized cancer, and in vitro diagnosis and ex vivo purging neoplastic cells from a biological fluid. RNAs and DNAs encode the monoclonal antibody, and a hybrid vector carrying the nucleotides and transfected cells express the peptides.
    Type: Grant
    Filed: October 8, 1993
    Date of Patent: August 28, 2001
    Assignee: Coulter Corporation
    Inventors: F. J. R. do Couto, R. L. Ceriani, J. A. Peterson
  • Patent number: 6159740
    Abstract: A method and apparatus for performing screening of obscured or partially obscured cells to enumerate one or more cell population subsets. For example, a whole blood sample or portion thereof is screened to provide the desired analysis of one or more white blood cell population subsets in the sample. A white blood cell population including the obscured subset of interest is first counted, along with a standard population. The standard population can be one of the total number of white blood cell populations, a second white blood cell population which does not obscure the shifted or non-shifted sensed characteristic of the subset of interest, an artificial population formed by microspheres which also do not obscure the shifted or non-shifted sensed characteristic of the subset of interest or a white blood cell population into which the sensed characteristic of the subset will be wholly or partially shifted.
    Type: Grant
    Filed: May 17, 1990
    Date of Patent: December 12, 2000
    Assignee: Coulter Corporation
    Inventors: James Carey Hudson, Thomas Russell, Carlos M. Rodriguez, Wallace H. Coulter
  • Patent number: 5871946
    Abstract: An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.
    Type: Grant
    Filed: May 18, 1995
    Date of Patent: February 16, 1999
    Assignee: Coulter Corporation
    Inventors: Frank J. Lucas, Gerald E. Jaffe, Steven Bott, James H. Carter
  • Patent number: 5861311
    Abstract: The invention describes the preparation of preserved, non-infectious control cells. The control cells are prepared by modification, activation or otherwise changing selected normal leukocyte cells, tissue culture cells or zenogenic transplants in immunosuppressed animals to have characteristics of abnormal cells or by the addition of cells from an established cell line, which has been similarly modified or activated, to a normal leukocyte population. The preferred method of preserving the control cells is by lyophilization using an isotonic 10% trehalose solution.
    Type: Grant
    Filed: July 29, 1996
    Date of Patent: January 19, 1999
    Assignee: Coulter Corporation
    Inventors: John A. Maples, Patricia A. Roth, Melissa J. Stiglitz
  • Patent number: 5849513
    Abstract: An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.
    Type: Grant
    Filed: July 31, 1997
    Date of Patent: December 15, 1998
    Assignee: Coulter Corporation
    Inventors: Gerald E. Jaffe, Frank J. Lucas, James H. Carter
  • Patent number: 5834315
    Abstract: A reagent composition which does not contain cyanide ions and a method for measuring hemoglobin concentration in a blood sample. In addition, the reagent composition can be used to measure hemoglobin concentration and differentiate at least two subpopulations of leukocytes from the same reaction product of the reagent composition and a blood sample. The reagent composition comprises at least one lysing agent selected from the group consisting of a quaternary ammonium salt, a pyridinium salt, and combinations thereof, in an amount effective to adequately lyse the erythrocytes and elute the hemoglobin, an antioxidant in an amount effective to convert the released hemoglobin into to a hemochromogen. The pH can be adjusting with a pH agent to provide a pH ranging from 5 to 11.5.
    Type: Grant
    Filed: December 23, 1994
    Date of Patent: November 10, 1998
    Assignee: Coulter Corporation
    Inventors: Mirta I. Riesgo, Carole Jo Young
  • Patent number: 5798217
    Abstract: The invention provides a method for enriching a sample in tumor cells and determining the DNA ploidy and proliferative index of the tumor cells present in the sample. The method uses a pan-leukocyte monoclonal antibody conjugated to a separable substrate such as magnetic microspheres to conjugate and remove leukocytes from the sample. A fluorescently labelled monoclonal antibody specific to a tumor associated antigen or an antigen arising from the presence of the tumor and a DNA staining reagent are added to the leukocyte depleted sample. The sample is then analyzed by dual color flow cytometry to determine cells having an abnormal DNA content by gating on fluorescently labelled cells.
    Type: Grant
    Filed: June 12, 1995
    Date of Patent: August 25, 1998
    Assignee: Coulter Corporation
    Inventors: Wade E. Bolton, Norma Sue Kenyon, Olavi Siiman, Robert J. Schmittling
  • Patent number: 5786224
    Abstract: A lytic reagent composition is provided which selectively stromatolyses red blood cells in a blood sample. In addition, a lytic reagent system is provided which enables the differentiation of at least three subpopulations of leukocytes. A method for using the lytic reagent system is also provided. Still further, the lytic reagent system finds use in the determination of the hemoglobin in the blood. The lytic reagent system utilizes an alkyl sulfate, polyoxyethylene based surfactant and acid with a hypertonic, alkaline, stabilizing reagent. The system and analysis method maintains the cellular morphology of the leukocytes and can be used to analyze normal and abnormal blood samples, fresh and aged blood, human and non-human animal blood samples.
    Type: Grant
    Filed: June 29, 1995
    Date of Patent: July 28, 1998
    Assignee: Coulter Corporation
    Inventors: Yi Li, Carole Young, Timothy J. Fischer
  • Patent number: 5783673
    Abstract: A method for preparing phycobiliprotein/amine-reactive dye conjugates is disclosed in which the conjugates so prepared overcome the energy transfer/fluorescent quenching dilemma encountered in the use of prior art conjugates. A phycobiliprotein, for example, phycoerythrin or allophycocyanin, is conjugated with an amine-reactive dye, for example, Texas Red or carboxyfluorescein succinimidyl ester, in the presence of a selective salt which causes a hydrophobic intramolecular rearrangement of the phycobiliprotein thereby exposing more hydrophobic sites for binding to the amine-reactive dye. The conjugates prepared according to the invention are useful in multiple color fluorescence assays without requiring the use of multiple exciting sources.
    Type: Grant
    Filed: October 10, 1996
    Date of Patent: July 21, 1998
    Assignee: Coulter Corporation
    Inventor: Ravinder K. Gupta
  • Patent number: 5776720
    Abstract: An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.
    Type: Grant
    Filed: May 18, 1995
    Date of Patent: July 7, 1998
    Assignee: Coulter Corporation
    Inventors: Gerald E. Jaffe, Frank J. Lucas, James H. Carter
  • Patent number: 5776706
    Abstract: Gelatin and aminodextran coated polymer core particles useful in immunoassays and methods of making the same are disclosed. The preparation of aminodextrans having varying amounts of amine groups is also described, as is a method of crosslinking gelatin and aminodextran without the use of a stabilizer.
    Type: Grant
    Filed: March 6, 1997
    Date of Patent: July 7, 1998
    Assignee: Coulter Corporation
    Inventors: Olavi Siiman, Alexander Burshteyn, Ravinder K. Gupta
  • Patent number: 5763204
    Abstract: Preparation of non-infectious control cells from normal, non-infectious, non-disease altered blood by depletion or augmentation of one or more cell types found in normal blood to reflect a specific disease state. The control cells so produced are preserved and thereafter reconstituted for use in immunological assays.
    Type: Grant
    Filed: May 28, 1996
    Date of Patent: June 9, 1998
    Assignee: Coulter Corporation
    Inventors: John A. Maples, Robert H. Raynor, Olavi Siiman, Melissa J. Stiglitz, Stephen F. Healy, Jr.
  • Patent number: 5733719
    Abstract: An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.
    Type: Grant
    Filed: May 18, 1995
    Date of Patent: March 31, 1998
    Assignee: Coulter Corporation
    Inventors: Gerald E. Jaffe, Frank J. Lucas, James H. Carter
  • Patent number: 5707877
    Abstract: The invention relates generally to colloidal particle having a core material and a gelatin/aminodextran coating with pendent functional groups attached thereto. Biological substances or molecules, especially monoclonal antibodies, may be attached to said particles. The monoclonal antibody containing particles are useful in a variety of positive and negative biological assays.
    Type: Grant
    Filed: June 23, 1995
    Date of Patent: January 13, 1998
    Assignee: Coulter Corporation
    Inventors: Olavi Siiman, Alexander Burshteyn, Ravinder K. Gupta
  • Patent number: 5698411
    Abstract: A method for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound is also disclosed.
    Type: Grant
    Filed: May 18, 1995
    Date of Patent: December 16, 1997
    Assignee: Coulter Corporation
    Inventors: Frank J. Lucas, Gerald E. Jaffe, Steven E. Bott, James H. Carter
  • Patent number: 5692220
    Abstract: A system and method of diagnosis provides that data from a hematopathology analyzer, e.g., hematology analyzer and/or other blood testing instrument or test, is input and compared with patterns corresponding to specific patient conditions. The matched patterns are arranged in a hierarchy in accordance with predetermined rules. Initially the patterns matched are displayed, optionally with recommendations for additional testing. If additional testing is done, a comparison of the original test data and new data is done, the patterns matched and at least one highest ranked pattern is identified, optionally with a differential diagnosis.
    Type: Grant
    Filed: September 2, 1993
    Date of Patent: November 25, 1997
    Assignee: Coulter Corporation
    Inventors: Lawrence W. Diamond, Doyen T. Nguyen
  • Patent number: 5686308
    Abstract: A lytic reagent composition is provided which selectively stromatolyses red blood cells in a blood sample. In addition, a lytic reagent system is provided which enable the differentiation of at least two subpopulations of leukocytes. A method for using the lytic reagent system is also provided. Still further, the lytic reagent system find use in the determination of the hemoglobin in the blood. The lytic reagent system utilizes an ethoxylated long chain compound based lysis reagent with an acid and a hypertonic, alkaline, stabilizing reagent. The system and analysis method maintains the cellular morphology of the leukocytes and can be used to analyze normal and abnormal blood samples, fresh and aged blood, human and non-human animal blood samples, as well as other fluid samples such as bone marrow.
    Type: Grant
    Filed: June 8, 1995
    Date of Patent: November 11, 1997
    Assignee: Coulter Corporation
    Inventors: Yi Li, Carole Young, Timothy J. Fischer, James H. Carter
  • Patent number: 5658741
    Abstract: The invention describes the use of novel aminodextran compounds containing about 5-20% by weight amine groups to bind a plurality of monoclonal antibodies. The resulting antibody-aminodextran compounds may be used to induce the activation and proliferation of selected mammalian cells. Specific examples are given using an anti-CD3 monoclonal antibody conjugated two aminodextrans containing about 5% and 16%, respectively, by weight amine groups as an agent for inducing the activation and proliferation of T cells.
    Type: Grant
    Filed: July 19, 1995
    Date of Patent: August 19, 1997
    Assignee: Coulter Corporation
    Inventors: Wade E. Bolton, John A. Maples, Olavi Siiman, Norma Sue Kenyon, Cynthia G. Healy
  • Patent number: 5653686
    Abstract: A method and transfer device for transferring fluids between closed vials. The transfer device includes a cylinder having a pair of opposing open tubes or tubular portions into which a pair of vials can be inserted to transfer fluid therebetween. The tubes are separated by a wall through which extends a fluid transfer needle and a vent needle which first is inserted into a first vial to vent the first vial into a vent chamber or reservoir formed in the transfer device before the transfer needle is inserted into the first vial. The first vial, stopper first, is inserted into first tube until seated onto an exposed end of the transfer needle. A second vial, stopper first, then is inserted into the second tube until seated onto the opposite exposed end of the transfer needle. A pressure differential between the closed vials preferably can be utilized to transfer a desired fluid portion between the vials.
    Type: Grant
    Filed: January 13, 1995
    Date of Patent: August 5, 1997
    Assignee: Coulter Corporation
    Inventors: Wallace H. Coulter, Charles R. Shambaugh