Abstract: The present invention discloses a SERS-nanotag comprising gold nanoparticle, an encapsulating agent, a Raman reporter and an antibody. The present invention also discloses a diagnostic kit consisting of SERS-nanotags for identification of breast cancer biomarker selected from the group consisting of Estrogen Receptor (ER), Progesterone Receptor (PR), human epidermal growth factor receptor 2 (HER2) and Ki67, simultaneously in abreast cancer tissue sample using a surface enhanced Raman scattering signature peaks. The multiplexing Raman peak pattern provides the presence of multiple biomarkers at a time in heterogeneous paraffin embedded breast cancer tissue samples with a concentration level of the SERS-nanotags by applying single laser (532 nm/633 nm/785 nm) revealing simultaneous Raman peaks for the respective biomarkers.

Abstract: A nanoadsorbent based filter is used for purification of fluoride and arsenic contaminated water. 140-150 g low cost (˜10 USD/kg) nanoparticles of gamma alumina of 20-25 mg/g fluoride and 25-30 mg/g arsenic adsorption capacity is incorporated in propylene filter without susceptibility of leaching incorporated nanoparticles in water. The cost of domestic defluoridation device containing low cost nanoalumina incorporated filters/cartridges along with housing, overhead tank, tubing and treated water storage container etc. is of very low cost of around 25 USD/device. The fluoride treatment cost would be <0.5 USD/100 lit for 4-5 mg/l fluoride water after 2-3 regenerations while, the arsenic treatment cost using domestic filtration device would be <0.25 USD/100 lit for 90-100 ?g/l arsenic (III) water.

Abstract: The current invention relates to a polymer-lipid microemulsion delivery system for drugs or antiviral compounds used in the treatment or inhibition of viral Acute Respiratory Distress Syndromes (ARDS), a process for producing the microemulsion delivery system, and to methods of use of the microemulsion delivery system for the treatment of ARDS.

Abstract: The present disclosure relates to a polypeptide exhibiting granulocyte-colony stimulating factor activity. The polypeptide comprises at least one non-native cysteine residue at a site selected from the group consisting of T1CP2 (SEQ ID NO: 25), P2CL3 (SEQ ID NO: 26), L3CG4 (SEQ ID NO: 27), G4CP5 (SEQ ID NO: 28), P5CA6 (SEQ ID NO: 29), A6CS7 (SEQ ID NO: 30), S96CP97 (SEQ ID NO: 31), P97CE98 (SEQ ID NO: 32), L99CG100 (SEQ ID NO: 33), P101CT102 (SEQ ID NO: 34), E122CE123 (SEQ ID NO: 35), L124CG125 (SEQ ID NO: 36), M126CA127 (SEQ ID NO: 37), P138CA139 (SEQ ID NO: 39), A143CF144 (SEQ ID NO: 40), R146CR147 (SEQ ID NO: 41), R169CH170 (SEQ ID NO: 42), H170CL171 (SEQ ID NO: 43), L171CA172 (SEQ ID NO: 44), A172CQ173 (SEQ ID NO: 45), and Q173CP174 (SEQ ID NO: 46) in an amino acid sequence having at least 90% sequence identity to sequence set forth in SEQ ID NO: 2.

Abstract: The disclosure provides methods for the measurement of ground water characteristics. The carbon dot tracer is injected in an injection bore well. Carbon dot tracer and ground water are thoroughly mixed in the injection bore well. The ground water mixed with carbon dot tracer is pumped from the injection bore well to an acquiring bore well connected with injection bore well with micro and major fracture; the water samples mixed with carbon dot tracer were collected at the acquiring bore well. The characteristics of the ground water were measured by measuring fluorescence. The disclosure further relates to a method for depicting the solute transportation in the soil.

Abstract: Decalin derivative compounds of formula (I) are provided, including processes for preparation thereof, and pharmaceutical compositions including the decalin derivative compounds. Methods are provided for treating blood related disorders, such as sickle cell anemia, in a subject in need thereof using a compound of formula (I).

Abstract: The present invention relates to an indole compound of Formula (I) or pharmaceutically acceptable salt thereof, and process for the preparation thereof. The present invention also relates to a pharmaceutical composition of indole compound of Formula (I) or pharmaceutically acceptable salt thereof for treating mycobacterial infection or antimalarial infection or antifungal infection in a subject in need thereof.

Abstract: The present invention provides a water soluble polymer surfactant (PS-DGlu) of formula I which is utilized for synthesis of functionalized polystyrene nanobead covalently incorporating (oligo) p-phenylenevinylene (OPV) nanosensor (PSG-OPV-n) which in turn is useful for the detection of bilirubin in human serum. The present invention further provides a process for the preparation of the water soluble polymer surfactant (PS-DGlu) of formula (I) and a mini emulsion polymerization process for the synthesis of PSG-OPV-n. wherein, n is 30-50. The PSG-OPV-n nanosensor beads show selectivity towards detection of bilirubin in presence of interferences such as glucose, sucrose, metal ions, cholesterol, and biliverdin with limit of detection of 20 nM. Ultimately, the invention also provides a kit for visual detection of bilirubin in human serum.

Abstract: The present invention disclosed a continuous flow and batch process for the separation of metal or metal oxide nanoparticles continuously in a periodic manner at the liquid-liquid interface using a centrifugal separator cum extractor, wherein the nanoparticles are collected at the liquid-liquid interface of the polar and non-polar liquids.

Abstract: Disclosed herein is a composite material that is formed from a polymer, acetylated collagen and graphene, which can be used as a super-capacitor material. Also disclosed herein are methods of making said composite material and its intermediates, as well as a supercapacitor made using said material.

Abstract: The novel process enables designing of raw materials and processing parameters, enabling synergistic and simultaneous chemical reactions among the various reactants of the design mix of chemical precursor of brine sludge which includes barium sulphate, magnesium hydroxide, calcium carbonate, sodium chloride, silica, aluminum containing compounds necessary for developing highly efficient shielding phases leading to homogenous matrix of shielding materials.

Abstract: The present invention relates to a purification process of recombinant antibody fragments from inclusion bodies expressed in microbial cells. More particularly, the present invention relates to a process for purification of recombinant humanized (rHu) antibody fragment, Ranibizumab from inclusion bodies expressed in microbial cells.

Abstract: The present invention relates to the process for the preparation of Pyrylium salts having the formula represented below. Present invention provide a simplified method of producing symmetrical and unsymmetrical pyrylium salts. The invention explores readily available starting materials with reaction conditions which are suitable for industrial scale applications. All the synthesized compounds were confirmed by various spectroscopic techniques such as Fourier transform infrared spectroscopy, 1H NMR, 13C NMR, 19F NMR spectroscopy, and single-crystal X-ray analysis. Mass of the compounds confirmed by HRMS analysis.

Abstract: A process and catalyst are provided for the non-oxidative dehydrogenation of propane for the production of propylene as petrochemical building blocks. The process provides a direct single-step gas-phase dehydration of propane mixed with nitrogen in the presence and absence of steam/hydrogen over supported bimetallic alumina-silicates zeolites. The catalyst contains no precious metal entities and may contain one metal from group VIB in combination with another metal from group IIIA or IVA supported on FAU, MFI, KFI, BEA type alumina-silicates zeolites. The process provides a propane conversion of 18% to 52% with a propylene yield of 10% to 25%.

Abstract: Engineered FnCas9 variants are provided that have an enhanced kinetic activity and a broader PAM recognition. The protein engineering methodology introduced specific mutations that stabilized interaction between Cas9 enzyme and target DNA. The enhanced kinetic activity increases NHEJ-mediated editing, owing to more efficient DSB generation potential than WT FnCas9, and the broadened PAM specificity increases the target range of FnCas9 variants. Thus, the scope and accessibility of CRISPR-Cas9 system targets are widened, along with generating robust and highly specific engineered FnCas9 variants.

Abstract: UV shielding bio-derived furanic polymers (BFP) and UV-shielding composite films containing BFP having another polymer of natural or synthetic origin at varying concentration with high thermal stability, mechanical stability and elasticity are prepared through solvent evaporation casting. A process for preparing BFP having varying physicochemical properties can be carried out via dehydration of various biomass saccharides in different solvents employing various catalysts. The resulting brown-colored films show excellent UV shielding in the region 200 nm to 400 nm and exhibit high optical transparency. The UV shielding efficiency of the film increases with an increase in its treatment temperature. The films are stable and durable in terms of mechanical stability and elasticity even after exposing to harsh conditions without affecting their UV-shielding efficiency.

Abstract: The present invention discloses a photocatalytic process and methodology for the preparation of acetic acid by a hydrocarboxylation reaction of methanol using carbon dioxide under visible light irradiation. Importantly, the reaction occurred under ambient temperature and pressure condition using a readily available household LED lamp in the presence of a transition metal based molecular photocatalyst, homogeneous as well as supported to semiconductor support and a CO2-philic solvent without adding any external electron and proton donors.

Abstract: The present invention provides an improved process for inducing the expression of the light chain and heavy chain of the said rHu biosimilar Ranibizumab by employing natural sugars such as lactose and galactose. The replacement of IPTG with natural sugars overcomes the regulatory limitation of synthetic element trace contamination in the final drug substance and reduces the burden on the recombinant host cell.

Abstract: A method for optimally producing a holographic image using a Holographic Optical Element (HOE) and the HOE meant for controlling directions and divergences of light beams to impart system compactness. The system uses concave and convex lenses and other beam expanding, splitting, modulating and combining optics for realization of compactness and high throughput. The thin laser beam is split using a holographic optical element and a conventional beam splitter. A neutral density filter adjusts the intensity of a reference beam to match the intensity of an object beam so that high quality digital holograms can be recorded. Effects of vibrations are minimized by the compact optical design, by anti-vibration mounts, by mounting all the opto-mechanical components on a single rigid platform and by enclosing the system. An electro-optical sensor array records holograms digitally and an algorithm numerically reconstructs and further quantifies the results using a personal computer/laptop/tablet etc.

Abstract: The present invention relates to a novel cloning, expression and refolding process for preparing antibody fragments. More particularly, the present invention relates to a cloning, expression and refolding platform for preparing recombinant humanized (rHu) Ranibizumab.