Abstract: A process for the partial or complete separation of glycosylated and nonglycosylated proteins is described, in which: a) a triazine dye immobilized on a matrix is incubated with a mixture of glycosylated and nonglycosylated proteins, b) the matrix is then washed to remove the unbound proteins, and c) the proteins are eluted by means of a stepwise or continuous increase in the ionic strength or in the pH, nonglycosylated proteins and proteins having an increasing degree of glycosylation being collected separately from one another in the eluate fractions obtained. By means of the use of this process it is possible, for example, to prepare a human albumin which is free of glycoside bonds and is expressed in yeast cells.
Type:
Grant
Filed:
December 14, 1999
Date of Patent:
June 14, 2005
Assignee:
Delta Biotechnology Ltd.
Inventors:
Jürgen Römisch, Jörg Weisse, Harald Stauss, Annette Feussner
Abstract: The present invention relates to a process for purifying the protein human serum albumin extracted from serum or recombinant human albumin produced by transforming a microorganism with a nucleotide coding sequence encoding the amino acid sequence of human serum albumin. The present invention provides highly purified albumin.
Type:
Grant
Filed:
February 24, 1999
Date of Patent:
October 28, 2003
Assignee:
Delta Biotechnology Ltd.
Inventors:
Andrew Robert Goodey, Darrell Sleep, Hendrik Van Urk, Stephen Berezenko, John Rodney Woodrow, Richard Alan Johnson, Patricia Carol Wood, Stephen James Burton, Alan Victor Quirk
Abstract: The use of a means to vary Ubc4p or Ubc5p activity in a fungal cell to control the copy number of a plasmid in the cell. The level of Ubc4p or Ubc5p activity may be reduced/abolished (for example by gene deletion, mutagenesis to provide a less active protein, production of antisense mRNA or production of competitive peptides) to raise the copy number and increase yield of a protein encoded by the plasmid.
Abstract: A method of modulating cellular proliferation by the application of a thymidine phosphorylase to an organism. In a further aspect of the subject method, the thymidine phosphorylase is a conjugate which includes a targeting portion adapted to target the conjugate to a specific cell type or anatomical location. The thymidine phosphorylase has a thymidine phosphorylase activity of at least about 5%, preferably at least about 50% and, most preferably, at least about 90%, of the native E. coli enzyme.
Type:
Grant
Filed:
January 11, 1996
Date of Patent:
September 18, 2001
Assignee:
Delta Biotechnology Ltd.
Inventors:
David J. Ballance, Michael G. Courtney, Christopher J. A. Finnis, Darrell Sleep
Abstract: Reduction (preferably elimination) of the HSP150 protein in a yeast used to produce desired foreign proteins, especially human albumin, facilitates purification of the protein.
Type:
Grant
Filed:
February 20, 1997
Date of Patent:
July 21, 1998
Assignee:
Delta Biotechnology Ltd.
Inventors:
Patricia Carolyn Wood, Alan Victor Quirk