Patents Assigned to DH Technologies Development Pte. Ltd.
  • Patent number: 10068752
    Abstract: Systems and methods for multiplexed precursor ion selection are provided. A mass isolator includes a selection region of rods, a transmission region of rods, and a barrier electrode lens separating the selection and transmission regions. Two or more different precursor ions are selected by applying two or more different AC voltage frequencies to rods of a selection region in order to resonate the two or more different precursor ions from a continuous beam of ions. The two or more different precursor ions are transmitted by applying a DC voltage to the barrier electrode lens, creating an electric field potential barrier over which only the resonating ions are transmitted. Precursor ions of product ions from combined product ion spectra produced by multiplexed precursor ion selection are identified by grouping the target precursor ions.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: September 4, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventor: Takashi Baba
  • Patent number: 10068753
    Abstract: A transmission window that has a constant rate of precursor ion transmission for each precursor ion is stepped across a mass range, producing a series of overlapping transmission windows across the mass range. The precursor ions produced at each step are fragmented. Resulting product ions are analyzed, producing a product ion spectrum for each step of the transmission window and a plurality of product ion spectra for the mass range. For at least one product ion of the plurality of product ion spectra, a function that describes how an intensity of the at least one product ion from the plurality of product ion spectra varies with precursor ion mass as the transmission window is stepped across the mass range is calculated. A precursor ion of the at least one product ion is identified from the function. An elution profile can also be determined from the function.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: September 4, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Nic G. Bloomfield, Frank Londry
  • Patent number: 10062556
    Abstract: A method and apparatus for conducting reactions between precursor ions and reagent ions, for example, a reaction between a precursor cation and an electron, such as ECD, are disclosed. The apparatus comprises first, second, and third pathways, each of which extends at least partially along a central axis, and wherein the second central axis is orthogonal to the first and third central axes. Charged species can be introduced into the second pathway as the ions are transmitted therethrough, thereby increasing precursor ion and charged species interaction without simultaneous trapping of the species.
    Type: Grant
    Filed: December 21, 2015
    Date of Patent: August 28, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventor: Takashi Baba
  • Publication number: 20180218895
    Abstract: A method and apparatus for clearing ions from a multipole ion transmission device which includes introducing a DC or RF clear out pulse to one or more of the rods of the multipole device. The DC pulse is selected so as to supply sufficient kinetic energy to the ions to overcome a pseudo-potential trapping well generated by the RF potentials of the ion transmission device. For an RF pulse, the auxiliary RF signal uses frequencies that correspond to the ejected ion's frequencies of motion. In select embodiments, the multipole device can be a quadrupole or the apparatus can be part of a tandem mass spectrometer.
    Type: Application
    Filed: November 28, 2014
    Publication date: August 2, 2018
    Applicant: DH Technologies Development PTE Ltd.
    Inventors: Bruce Andrew Collings, John Vandermey
  • Patent number: 10032614
    Abstract: A method and apparatus for analyzing samples using mass spectrometry are disclosed. The apparatus includes a reaction device configured to dissociate sample ions into fragments by reacting the sample ions with a charged species (e.g., electrons) such as through ECD, EID, or EIEIO. The kinetic energy of the charged species is such that the fragments may be detected and produce spectra that allow for the determination of isomeric species in the sample and the location of double bonds of sample molecules. The fragments may include radical fragments and non-radical fragments. The apparatus may also include an oxygen gas source configured to react with the radical fragments to produce oxygen-radical fragments. Spectra resulting from analysis of the fragments may allow for the determination of the oxygen-radical fragments resulting from the dissociation of the sample molecules.
    Type: Grant
    Filed: June 4, 2015
    Date of Patent: July 24, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Takashi Baba, John Lawrence Campbell
  • Patent number: 10014166
    Abstract: A method and apparatus for conducting ion to charged species reactions, more particularly reactions wherein the charged species is an electron, such as ECD. The apparatus comprises first and second pathways which are orthogonal to one another. The first pathway through which ions are introduced comprises multiple multipoles with a gap situated there between. The second pathway introduces the charged species through the gap orthogonally to the first pathway. In this way, a cross-type reaction device allows ion-charged species interactions to occur.
    Type: Grant
    Filed: May 29, 2014
    Date of Patent: July 3, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventor: Takashi Baba
  • Patent number: 10008376
    Abstract: The present teachings are directed to methods and systems for the selection of ions for subsequent ion fragmentation in the analysis of a sample. Rather than select the most intense subset of precursor ions for further analysis in an attempt to maximize the number of high quality, identifiable MS/MS spectra, in some settings, systems and methods for analyzing and 5 identifying precursor ions for further processing can benefit from a discovery approach in which precursor ions are selected randomly/stochastically.
    Type: Grant
    Filed: October 27, 2015
    Date of Patent: June 26, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Nic Bloomfield, Stephen A. Tate
  • Patent number: 10001456
    Abstract: Methods and systems for separating and/or quantifying peptides using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicant's teachings, the methods and systems can provide for the separation of one or more peptides, for example, peptides that may be difficult to separate with conventional techniques, such as mass spectrometry (MS), by complexing the peptides with a metal cation (e.g., Ca2+) prior to DMS. In some aspects, the present teachings can prevent proton stripping from ionized peptides that can occur in DMS to prevent unintended and/or undesirable alterations to the peptide's charge state distribution.
    Type: Grant
    Filed: August 28, 2015
    Date of Patent: June 19, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: John L Campbell, Yves LeBlanc, Chang Liu
  • Patent number: 9997340
    Abstract: A mass spectrometer apparatus and method for conducting simultaneous MS/MS analysis including: a device to select a precursor ion having a specified m/z; a gas-filled collision cell; an RF-only multipole mass spectrometer, the mass spectrometer having a generator attached thereto for generating at least two auxiliary AC fields in the RF-only multipole mass spectrometer; a gate for providing a repulsive DC or AC barrier downstream to an exit of the RF-only multipole mass spectrometer; an ion detection system situated downstream from the DC or AC barrier for measuring an ion current derived from ions that overcome the repulsive barrier. The mass spectrometer may also be configured so that each of the auxiliary AC fields are generated by the introduction of individual auxiliary AC frequencies and each frequency is amplitude modulated at a unique frequency.
    Type: Grant
    Filed: September 12, 2014
    Date of Patent: June 12, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: James Hager, Christopher M Lock
  • Patent number: 9991104
    Abstract: Systems and methods are provided to perform dead time correction. An observed ion count rate is obtained using a non-paralyzable detection system of a mass spectrometer. The detection system includes an ion detector, a comparator/discriminator, a mono-stable circuit and a counter. The non-paralyzable detection system exhibits dead time extension at high count rates. The extension of the dead time occurs because the mono-stable circuit requires a rising edge to trigger and can only be triggered again after the output pulse from the comparator/discriminator has gone low. This allows a second comparator/discriminator pulse arriving just before the end of the dead time started by a first comparator/discriminator pulse to extend the dead time to the trailing edge of the second comparator/discriminator pulse. A true ion count rate is calculated by performing dead time correction of the observed ion count rate.
    Type: Grant
    Filed: April 19, 2013
    Date of Patent: June 5, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Bruce Andrew Collings, Martian Dima, Gordana Ivosev
  • Patent number: 9978575
    Abstract: Sample molecules are ionized producing a beam of ions using an ion source. A plurality of ion extractions are performed on the beam of ions using a TOF mass spectrometer. Electrical detections from each extraction are measured using an ADC, producing a mass sub-spectrum for each extraction. An ion m/z from the plurality of mass sub-spectra is selected. For each mass sub-spectrum, the amplitude and m/z of an ion within a m/z tolerance of the ion m/z is assigned to the corresponding amplitude band of a plurality of predetermined amplitude bands, producing a plurality of amplitude and m/z values for the each amplitude band. For each amplitude band of the plurality of predetermined amplitude bands, the plurality of amplitude and m/z values are combined into a peak, resulting in a plurality of peaks corresponding to the plurality of predetermined amplitude bands.
    Type: Grant
    Filed: September 21, 2015
    Date of Patent: May 22, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventor: Nic G. Bloomfield
  • Patent number: 9978576
    Abstract: Systems and methods are provided for time-of-flight analysis of a continuous beam of ions by a detector array. A sample is ionized using an ion source to produce a continuous beam of ions. An electric field is applied to the continuous beam of ions using an accelerator to produce an accelerated beam of ions. A rotating magnetic and/or electric field is applied to the accelerated beam to separate ions with different mass-to-charge ratios over an area of a two-dimensional detector using a deflector located between the accelerator and the two-dimensional detector. An arrival time and a two-dimensional arrival position of each ion of the accelerated beam are recorded using the two-dimensional detector. Alternatively, an electric field that is periodic with time is applied in order to sweep the accelerated beam over a periodically repeating path on the two-dimensional rectangular detector.
    Type: Grant
    Filed: September 22, 2017
    Date of Patent: May 22, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventor: Robert Alois Grothe, Jr.
  • Patent number: 9970900
    Abstract: Systems and methods are provided for selectively filtering 1,2-diolein and 1,3-dioleine ions from an olive oil sample. An ion source is instructed to ionize a mixture of an olive oil sample and a pre-ionization modifier. The pre-ionization modifier includes silver (Ag). A differential mobility spectrometry (DMS) device is instructed to separate ions received from the ion source and affected by a post-ionization modifier based on ion mobility. The second post-ionization modifier is butanol, for example. The DMS device is instructed to selectively filter separated 1,2-diolein precursor ions by selecting a first compensation voltage (CoV) for the DMS device. The first CoV is specific to separate 1,2-diolein precursor ions from 1,3-diolein precursor ions. The DMS device is instructed to selectively filter separated 1,3-diolein precursor ions by selecting a second CoV for the DMS device. The second CoV is specific to separate 1,3-diolein precursor ions from 1,2-diolein precursor ions.
    Type: Grant
    Filed: July 24, 2015
    Date of Patent: May 15, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Stefanie Kreppenhofer, Axel Besa
  • Patent number: 9970942
    Abstract: Methods and systems for detecting allergens using mass spectrometry are provided herein. In some aspects, a sample can be screened for the presence or quantity of ovalbumin, lysozyme, casein (isoform S1 and S2), lactoglobulin, high and low glutens, wheat, rye, oats, barley, mustard, sesame, and various types of nuts including macadamia, pistachio, brazil, walnuts, peanuts and hazelnuts by detecting one or more peptides specific to the allergen of interest using selected MRM transitions.
    Type: Grant
    Filed: September 4, 2012
    Date of Patent: May 15, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Stephen J. Lock, Subhasish Purkayastha, Brian L. Williamson, Keling Dong
  • Patent number: 9964552
    Abstract: A method is described for mass spectrometric analysis, detection and quantification of catecholamines. The methods can comprise reacting the catecholamines with a 4-aminoantipyrine reagent and detecting and/or quantifying the adduct produced by the reaction. The methods can also allow for multiplexing. Compounds formed by the reactions are also provided.
    Type: Grant
    Filed: October 22, 2014
    Date of Patent: May 8, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Subhakar Dey, Subhasish Purkayastha
  • Publication number: 20180122627
    Abstract: Systems and methods described herein utilize an ion guide for use in mass spectrometer systems, which ion guide can receive ions from an ion source for transmission to downstream mass analyzers, while preventing debris (e.g., unsolvated droplets, neutral molecules, heavy charged clusters) from being transmitted into a high-vacuum chamber of the mass spectrometer system. In various aspects, systems and methods in accordance with the present teachings can increase throughput, improve the robustness of the system, and/or decrease the downtime typically required to disassemble/clean sensitive components within the high-vacuum portions of the mass spectrometer system.
    Type: Application
    Filed: March 22, 2016
    Publication date: May 3, 2018
    Applicant: DH Technologies Development Pte. Ltd.
    Inventor: Mircea Guna
  • Patent number: 9941104
    Abstract: Methods and systems for delivering a liquid sample to an ion source are provided herein: In various aspects, the methods and systems described herein can utilize the flow provided by an LC pump(s) to drive a calibration fluid to an ion source of a mass spectrometer system. In various aspects, methods and systems described herein can additionally or alternatively be placed upstream of an LC column for providing an elution gradient of a plurality of solvents, without requiring a plurality of pumps and/or separate mixing elements.
    Type: Grant
    Filed: November 18, 2014
    Date of Patent: April 10, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventor: Peter Kovarik
  • Patent number: 9927396
    Abstract: Aspects of the innovations presented herein relate to improved systems that in some embodiments perform capillary electrophoresis (CE) and CE in conjunction with electrospray ionization (ESI) as an input to a mass spectrometry system (MS). Some embodiments use a high voltage isolated CE power supply that is configured to float on the high voltage output of an ESI-MS power supply, with a protective resistance in the ESI-MS path, as well as DC/DC converter isolation and communication system isolation for the isolated CE power supply. Some embodiments additionally use a cartridge assembly integrating separation and conductive fluid capillaries with fluid cooling and protective retractable housings for the capillary end portions and for the ESI output. The protective housing may further be used with an adapter for interfacing with different MS systems.
    Type: Grant
    Filed: October 26, 2012
    Date of Patent: March 27, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Brian D. Peterson, Dale Alan Owens, Peter S. Kotowski, Sunil S. Deliwala, Craig A. Hudson, James H. Reyes, Clive Cheung, Tom Tran, Anna X. Lou, Jane Luo, Jean-Marc Rene Lucien Busnel, Chitra K. Ratnayake
  • Patent number: 9921183
    Abstract: Differential mobility spectrometry is performed under vacuum. Ions generated in a high pressure region are received from the inlet orifice of a vacuum chamber using a first ion guide located in the vacuum chamber. The first ion guide focuses the generated ions on a DMS device inlet end using a plurality of tapered electrodes. The DMS device is coaxial and adjacent to the first ion guide. The DMS device separates the focused ions using a plurality of electrodes. The inscribed diameter at the DMS device inlet end is larger than the inscribed diameter at the first ion guide exit end to maximize ion transfer. The separated ions are received from the DMS device using a second ion guide coaxial and adjacent to the DMS device. The second ion guide focuses the separated ions on an exit orifice of the vacuum chamber using a plurality of tapered electrodes.
    Type: Grant
    Filed: December 6, 2014
    Date of Patent: March 20, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Bradley B. Schneider, Hassan Javaheri, Thomas R. Covey
  • Patent number: 9891203
    Abstract: Systems and methods are provided for detecting host cell contaminants in a protein biotherapeutic product using sequential windowed acquisition tandem mass spectrometry. Sequential windowed acquisition is performed on a protein biotherapeutic product sample by sequentially stepping a precursor mass window across a mass range, fragmenting transmitted precursor ions of each stepped precursor mass window, and analyzing product ions produced from the fragmented transmitted precursor ions. The sequential windowed acquisition is performed without any information about contaminating proteins before data acquisition, and produces data for every product ion of every transmitted precursor ion for the mass range. One or more measured product ion spectra are received, and compared to a library of host cell proteins. One or more host cell contaminants are detected by reporting host cell proteins from the library that match the one or more measured product ion spectra.
    Type: Grant
    Filed: June 3, 2014
    Date of Patent: February 13, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventor: Eric Johansen