Patents Assigned to ENZYNOMICS CO. LTD.
  • Polypeptide specifically binding to Taq DNA polymerase and use thereof
    Patent number: 10793900
    Abstract: The present invention discloses a novel polypeptide capable of binding Taq DNA polymerase, a polynucleotide encoding the polypeptide, a recombinant vector comprising the polynucleotide, a cell having the recombinant vector introduced therein, a method of producing the polypeptide using the recombinant cell, and a hot-start PCR composition comprising the polypeptide. The polypeptide of the present invention is capable of binding specifically to Taq DNA polymerase to inhibit the activity of the polymerase, and thus may be effectively used in hot-start PCR using Taq DNA polymerase.
    Type: Grant
    Filed: April 21, 2017
    Date of Patent: October 6, 2020
    Assignees: ENZYNOMICS CO. LTD., KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Da-Eun Hwang, Yong Keol Shin, Palinda Ruvan Munashingha, So Yeon Park, Yeon-Soo Seo, Hak-Sung Kim
  • POLYPEPTIDE SPECIFICALLY BINDING TO TAQ DNA POLYMERASE AND USE THEREOF
    Publication number: 20180298422
    Abstract: The present invention discloses a novel polypeptide capable of binding Taq DNA polymerase, a polynucleotide encoding the polypeptide, a recombinant vector comprising the polynucleotide, a cell having the recombinant vector introduced therein, a method of producing the polypeptide using the recombinant cell, and a hot-start PCR composition comprising the polypeptide. The polypeptide of the present invention is capable of binding specifically to Taq DNA polymerase to inhibit the activity of the polymerase, and thus may be effectively used in hot-start PCR using Taq DNA polymerase.
    Type: Application
    Filed: April 21, 2017
    Publication date: October 18, 2018
    Applicants: ENZYNOMICS CO. LTD., KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Da-Eun HWANG, Yong Keol SHIN, Palinda Ruvan MUNASHINGHA, So Yeon PARK, Yeon-Soo SEO, Hak-Sung KIM
  • Method for controlling length of overhang of double stranded DNA
    Patent number: 9868971
    Abstract: Disclosed are methods and kits for controlling the length of overhangs at the ends of double stranded DNA based on a competitive inhibition. Further provided are ligase-independent methods for joining the two ends of DNA strands using the same. The present methods efficiently control the overhangs of ds DNA. This user-controlled overhangs supply a tool for efficient ligation in a ligase independent way and can be advantageously used in DNA recombinant technology such as cloning gene or DNA fragments of interest or PCR products.
    Type: Grant
    Filed: October 28, 2013
    Date of Patent: January 16, 2018
    Assignee: ENZYNOMICS CO. LTD.
    Inventors: Yong Keol Shin, Yunil Suh, Min Young Lee, Ah Rong Jeong
  • METHOD FOR CONTROLLING LENGTH OF OVERHANG OF DOUBLE STRANDED DNA
    Publication number: 20150376672
    Abstract: Disclosed are methods and kits for controlling the length of overhangs at the ends of double stranded DNA based on a competitive inhibition. Further provided are ligase-independent methods for joining the two ends of DNA strands using the same. The present methods efficiently control the overhangs of ds DNA. This user-controlled overhangs supply a tool for efficient ligation in a ligase independent way and can be advantageously used in DNA recombinant technology such as cloning gene or DNA fragments of interest or PCR products.
    Type: Application
    Filed: October 28, 2013
    Publication date: December 31, 2015
    Applicant: ENZYNOMICS CO. LTD.
    Inventors: Yong Keol SHIN, Yunil SUH, Min Young LEE, Ah Rong JEONG