Abstract: The invention relates to a method for determining interaction kinetics for an analyte. The method comprises first contacting a solution containing the analyte with immobilized ligand, or analogue thereof, immobilized on an optical sensor surface; monitoring the binding of the analyte to the immobilized ligand or analogue, wherein the binding is measured as a resulting change in a property of the surface; and automatically determining the interaction kinetics, which determining step includes first defining parts of the dissociation phase that contains kinetic information for fitting. The invention further relates to an analytical system for studying molecular interactions, which system is capable of performing the novel method, as well as a computer program product for performing the steps of the method.

Abstract: A device and method for detecting the level of foam in a reactor vessel monitors the intensity of the light or the movement of the light detected by a camera which monitors at least one light source positioned inside the reactor vessel or viewable through the reactor vessel.

Abstract: A method of determining the concentration of at least one analyte in a plurality of samples by sequentially subjecting each sample to an analysis cycle comprises contacting the sample or a sample-derived solution with a sensor surface supporting a species capable of specifically binding the analyte or an analyte-binding species, detecting the amount of binding to the sensor surface, and regenerating the sensor surface to prepare it for the next analytical cycle, and based on the detected binding to the sensor surface determining the concentration of analyte in each sample using virtual calibration data calculated for each analysis cycle from real calibration data obtained by contacting the solid phase with samples containing known concentrations of analyte.

Abstract: A system for blending solutions and a buffer solution is disclosed. In this system a switch valve is present capable of flowing one or more solutions, a low pressure pump for pumping the one or more solutions through the switch valve and a T-joint capable of receiving the one or more solutions through the low pressure pump and blending the one or more solutions with a buffer solution. A high pressure pump is present for collecting a blended solution.

Abstract: A Surface Plasmon Resonance (SPR) biosensor system comprising: a SPR sensor surface, an illumination unit arranged to direct a wedge shaped beam of light at a line shaped detection area on the SPR sensor surface transverse to the direction of propagation of light, and a detection unit with detection optics for directing light reflected from the SPR sensor surface onto a two-dimensional optical detector unit such that the angle of reflection is imaged along one dimension and the width of the detection area along the other, wherein the illumination unit is arranged to selectively direct the wedge shaped beam of light at two or more spaced apart line shaped detection areas on the SPR sensor surface transverse to the direction of propagation of light.

Abstract: The invention discloses a flexible bag for cell cultivation, comprising a cultivation compartment and at least one dialysis compartment, wherein the dialysis compartment(s) is/are delimited from the cultivation compartment by one or more dialysis membranes and is/are fluidically connected to a first and a second port in the bag.

Abstract: The present invention relates to a rotary valve and to a chromatography system comprising such a rotary valve. The rotary valve comprising a stator with an inner stator face, and a rotor with an inner rotor face arranged in sealing contact with the inner stator face, the rotor (1) is rotatably movable to a plurality of rotor positions about a rotational axis (RA) relative to the inner stator face (202), wherein the stator comprises ports and orifices and the rotor comprises two or more rotor interconnection paths for selective fluidic interconnection of said orifices with respect to the rotor position and wherein the rotor interconnection paths are arranged to in a rotary position connect the first orifice (S) to the fourth orifice (LF), the second orifice (W) to the third orifice (LE). And in a rotary position connect the eight orifice (SP) to the seventh orifice (PCFP). And in a rotary position connect the eight orifice (SP) to the fifth orifice (TC), the sixth orifice (FC) to the seventh orifice (PCFP).

Abstract: The present invention relates to a method for identification of specific target proteins in a protein sample following a detection procedure, such as a Western blotting procedure, wherein the membrane is probed with at least two primary antibodies directed against the same and/or different epitopes of the same target protein, and wherein specific binding to the target protein in a sample is differentiated from unspecific binding to the target protein by comparing the resulting sample patterns, such as bands or spot patterns, with each other. In a further step signals from the true target proteins are enhanced while signals resulting from unspecific binding are diminished.

Abstract: The invention discloses a method for predicting the pH error during mixing of an aqueous buffer comprising at least one weak acid species and/or at least one weak base species, which comprises the steps of: a) selecting a start composition of the buffer, giving start values for pH and/or buffer concentration; b) calculating the concentrations of all ionic species present in the buffer at a specified pH value from the total composition of the buffer and available dissociation constants; c) calculating the contribution of each of said ionic species to a total pH variance from the specified pH value, the buffer concentration, the calculated concentrations of the ionic species and variances in amounts of buffer components; d) calculating the pH variance, and; e) setting the variance or the square root of the pH variance as the pH error.

Abstract: The present invention provides a method of assaying a sample solution for the presence of a first analyte comprising: (a) providing a sensor surface having a ligand immobilized thereto; (b) flowing the sample solution over the sensor surface; and (c) detecting the presence or absence of binding of the analyte to the ligand on the sensor surface; wherein the contact time between the sample solution and the immobilized ligand is less than 15 seconds.

Abstract: The invention relates to valve system for controlling a process fluid within a liquid processing system. The valve system comprises a valve arrangement, a pneumatic control system, and a connector unit. When the valve arrangement is connected to the connector unit, two or more valves are formed, such that the pneumatic control system controls an open/close or pressure control mode of the valves. A pump diaphragm system is disclosed, as well as a system for purifying a biological material that comprises the valve system or the pump diaphragm system. Also discloses are methods of using the valve system or the pump diaphragm system in a process for the purification of a biological material.

Abstract: Disclosed is a fluid path network (100) comprising plural fluid paths (110) interconnected by plural nodal chambers (120), three or more of said plural paths opening into each of said chambers, and said chambers being adapted the accept a removable fluid path modifying insert (130/140/150 FIGS. 3a-3c). The network is employed in a chromatography system described herein.

Abstract: A method of screening a plurality of fluid samples for the presence of species capable of specifically binding to a binding partner immobilized on a sensing surface of a sensor is disclosed. The method comprises contacting each sample with the sensing surface and a reference surface, and subjecting the sensing surface responses obtained for all samples to a computational process which comprises fitting al responses to a model equation for the relationship between a response at the sensing surface and the corresponding response at the reference surface. In an iterative process residuals above a pre-determined threshold value are removed, the model equation is adjusted, and all remaining samples are refitted to the adjusted model equation until the model equation at least substantially converges. Residuals above the predetermined threshold value are considered as species specifically binding to the binding partner.

Abstract: The present invention relates to a method of evaluation of molecular binding interactions at a sensing surface, and more particularly to a method for evaluation of screening data obtained from an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface of a biosensor that is independent of interaction models. Preferably the biosensor is a SPR biosensor. The invention also relates to a biosensor system arranged to perform the method and a computer program arranged to control the operation of the biosensor system.

Abstract: A method and a system for improving mixing of content in a container 3 provided in a filter system 1, said method comprising providing a mixing loop 13 parallel to a recirculation flow path 11 of the filter system 1.

Abstract: A fluid distributor unit comprising a channel system where one or more inlet channels (2) starting on an inlet side (3) of the distributor unit branch out successively into several channels (6) ending on the other side of the distributor unit, called the outlet side (4), characterised in that said distributor unit is provided in one single body (1) by free form fabrication.

Abstract: The present invention relates to a method for performing liquid chromatography purification of one or more target molecules from a sample comprising: providing an eluent flow having one or more target molecules, measuring an output parameter indicative of the content of the one or more target molecules in the eluent flow, storing output parameter data, and dividing the eluent flow into consecutive eluent fractions, dividing the output parameter data into corresponding data fractions, in each data fraction obtaining a value indicative of characteristic behavior of the measured output parameter, identifying trends in the measured output parameter based upon the obtained value in consecutive data fractions, and identifying peak(s) in the measured output parameter correlated to eluent fractions based upon the identified trends, whereby information of identified peak(s) and correlated eluent fraction(s) can be presented and purified sample(s) from the eluent may be collected.

Abstract: The present invention relates to the preparation of liquid mixtures, and more particularly to the preparation of a liquid mixture, such as a buffer, wherein the conductivity of the liquid mixture is measured and the pH indirectly determined if the buffer concentration is known. Another object of the present invention is to provide a method of preparing a liquid mixture with a predetermined pH value by using conductivity as feedback control parameter.