Abstract: The presently claimed invention applies to a genetic material processing, manipulation and transfection method and related product. The claimed invention relates to a method for changing the inherited characteristics of a cell through micro-beam chromosome modification. In one preferred embodiment, improvements to ‘genomic surgery’ are applied to modify source cell genetic material (101). Upon micro-beam welding of combined genetic material (301), transfection takes place either by using a laser beam with a wavelength of 337 nm to perforate a hole (303) on receptor cell (305) or micro-tube control technology is then applied to carry combined genetic material (301) through the micro-pore (303) into the receptor cell (305), inserting the welded chromosome segment (301) by injection. Combined genetic material may be placed anywhere within receptor cell (305), including transfection through nuclear hole (307) into the cell nucleus (311).
Abstract: The presently claimed invention applies to a genetic material processing and manipulation method and related product. The claimed invention relates to a method for changing the inherited characteristics of a cell through micro-beam chromosome modification. In one preferred embodiment, improvements to ‘genomic surgery’ are applied to modify source cell genetic material (101). Source cell (106) is stabilized through micro-pipette (102) applied negative pressure. Excised genetic material (101) undergoes a pre-cutting manipulation step (205) to enhance subsequent genetic manipulation. In an additional embodiment genetic material manipulation is aided by a spindle cutting step (303). The presently claimed invention provides a high quality alternate approach to directed genetic recombination without requiring the use of restriction enzymes and is used for chromosomal repair, removal of defects and new organism creation.
Abstract: The presently claimed invention applies to a genetic material processing and manipulation method and related product. The claimed invention relates to a method for changing the inherited characteristics of a cell through chromosome processing treatment. In a preferred embodiment, ‘genomic surgery’ is applied to source genetic material 101. Micro-beam cutting takes place on target genetic material 103, which is moved by way of micro-beam transport to a destination location adjacent to desired target genetic material 105. Source and target genetic material are welded during a micro-beam welding step, resulting in a combined or new chromosome combination 125. The combined or new chromosome combination 125 is transferred into receptor cell by three ways. The presently claimed invention provides a high quality alternate approach to directed genetic recombination without requiring the use of restriction enzymes and is used for chromosomal repair, removal of defects and new organism creation.