Abstract: The present invention provides stabilization of an extracellular vesicle. Specifically, the present invention provides, for example, a method of stabilizing the extracellular vesicle including mixing an extracellular vesicle-containing sample with a saccharide and a chelating agent.

Abstract: One or more of HLA gene primers selected from the group consisting of the following (1) to (3): (1) one or more HLA-G gene primers which are (1a) a first primer including the nucleotide sequence of SEQ ID NO. 1 and/or (1b) a second primer including the nucleotide sequence of SEQ ID NO. 3, (2) one or more HLA-E gene primers which are (2a) a first primer including the nucleotide sequence of SEQ ID NO. 5 and/or (2b) a second primer including the nucleotide sequence of SEQ ID NO. 7, and (3) one or more HLA-F gene primers which are (3a) a first primer including the nucleotide sequence of SEQ ID NO. 9 and/or (3b) a second primer including the nucleotide sequence of SEQ ID NO. 11.

Abstract: Provided are a program and the like in which a cleaning efficiency can be improved. A program allows a computer to execute processing of: acquiring an image including a still image or a moving image that is obtained by imaging an inside of a medical facility with an imaging device provided in the medical facility, or operation content information relevant to operation content in the medical facility; specifying a part to be cleaned in the medical facility and a cleaning method, on the basis of the acquired image or the acquired operation content information; and outputting a cleaning command according to the specified part to be cleaned in the medical facility and the specified cleaning method.

Abstract: Provided is an information processing method capable of effectively using health record of each user including test results of various tests conducted at a medical institution or the like. A health record is stored in association with user identification information for each user in a database. If acquiring the user identification information read by a dispersed management system, a computer reads a health record corresponding to the acquired user identification information from the database. The computer outputs the read health record to the management system.

Abstract: A method to recover an extracellular vesicle at a high efficiency, including (a) and (b): wherein (a) is mixing (i) an extracellular vesicle-containing sample, (ii) particles on which a substance having an affinity to extracellular vesicle membrane is immobilized, and (iii) a polymer to give a mixture solution containing (i?) target particles bound to the extracellular vesicle via the substance and (ii?) the polymer; and (b) separating the target particles from the mixture solution. The method further includes reducing a viscosity of the mixture solution between (a) and (b). A method for analyzing an extracellular vesicle. A kit having (a) a polymer, (b) a substance having an affinity to the extracellular vesicle membrane, and (c) an enzyme capable of degrading a polymer.

Abstract: The present invention provides recovering an extracellular vesicle(s) having high purity from an extracellular vesicle-containing sample. Specifically, the present invention provides a method of recovering an extracellular vesicle(s), comprising: (1) treating an extracellular vesicle-containing sample with a chelating agent; and (2) separating the extracellular vesicle(s) from the extracellular vesicle-containing sample treated with the chelating agent.

Abstract: The present invention provides recovering an extracellular vesicle(s) having high purity from an extracellular vesicle-containing sample. Specifically, the present invention provides a method of recovering an extracellular vesicle(s), comprising: (1) treating an extracellular vesicle-containing sample with a chelating agent; and (2) separating the extracellular vesicle(s) from the extracellular vesicle-containing sample treated with the chelating agent.

Abstract: The present invention provides a method and means useful for recovering an extracellular vesicle. More specifically, the present invention provides the following (I) and (II). (I) A method for recovering extracellular vesicle, the method including: (1) mixing whole blood with a nonionic surfactant and a chelating agent to give a mixture solution containing the extracellular vesicle, the nonionic surfactant and the chelating agent; and (2) separating the extracellular vesicle from the mixture solution. (II) A blood collection vessel containing a nonionic surfactant and a chelating agent.

Abstract: Provided is a gate region estimation program and the like that estimate a gate region using a learning model. This gate region estimation program causes a computer to execute processing of: acquiring a group of scatter diagrams including a plurality of scatter diagrams each different in a measurement item that are obtained from measurements by flow cytometry; inputting the group of scatter diagrams acquired to a learning model trained based on teaching data including a group of scatter diagrams and a gate region; and outputting an estimated gate region obtained from the learning model.

Abstract: The present invention provides a technique for reducing contamination with impurities in a system of operating an extracellular vesicle. More specifically, the present invention provides a method of washing an extracellular vesicle and the like. The method includes washing the extracellular vesicle with a nonionic surfactant that is a chain compound containing a structure represented by —O—(—CH2—CH2—O—)x—H, wherein x is a value defined in the description.

Abstract: The present invention provides a factor capable of predicting an effect of a medicinal therapy on a cancer such as HER2 positive cancer. More specifically, the present invention provides a method of predicting the effect of the medicinal therapy on the cancer, comprising: (1) analyzing a methylation level of a cytosine residue in one or more CpG sites present within a nucleotide sequence in a promotor region, an untranslated region or a translated region of HSD17B4 gene in a sample taken from a human subject; and (2) predicting the effect of the medicinal therapy on the cancer based on the analyzed methylation level.

Abstract: A method involves recovery an extracellular vesicle from an extracellular vesicle-containing sample. Such a method of recovering the extracellular vesicle may include separating the extracellular vesicle from the extracellular vesicle-containing sample in the presence of a polymer.

Abstract: An indicator may have improved accuracy for predicting an effect of a medicinal therapy on breast cancer. The method may estimate a breast cancer cell existence ratio and other methods, the method including: (1) analyzing methylation level of (1a) a cytosine residue in a CG portion of nucleotides at positions 1,000 to 1,001 in a nucleotide sequence of SEQ ID NO: 1, (1b) a cytosine residue in a CG portion of nucleotides at positions 1,000 to 1,001 in a nucleotide sequence of SEQ ID NO: 2, (1c) a cytosine residue in a CG portion of nucleotides at positions 1,001 to 1,002 in a nucleotide sequence of SEQ ID NO: 3, or (1d) a combination of the cytosine residues, in a sample derived from a human subject; and (2) estimating the breast cancer cell existence ratio in the sample, based on the methylation level analyzed at (1).