Abstract: An image acquisition device is an image acquisition device that acquires an X-ray transmission image of an object conveyed in a conveyance direction, and the image acquisition device includes an X-ray irradiator that outputs an X-ray, a belt conveyor that conveys the object in the conveyance direction, an X-ray detection camera having a scintillator that converts an X-ray penetrating the object into scintillation light, a line scan camera that detects the scintillation light and outputs a detection signal, and an amplifier that amplifies the detection signal at a predetermined set amplification factor and outputs a amplified signal, a controller that generates an X-ray transmission image based on the amplified signal, and an amplifier controller that sets one of a first amplification factor or a second amplification factor corresponding to an amplification factor lower than the first amplification factor as the set amplification factor based on a predetermined imaging condition.