Abstract: Disclosed are methods, device kits, and systems for improved quantification of mRNA from whole blood. More particularly, the devices and kites related thereto are useful for the controlled and repeatable ex vivo stimulation of whole blood.
Type:
Grant
Filed:
October 31, 2011
Date of Patent:
January 28, 2020
Assignees:
Hitachi Chemical Co., Ltd., Hitachi Chemical Company America, Ltd.
Abstract: Some embodiments provide for a device that collects vesicles and vesicle-like materials from biological fluids. Such devices include at least one sample loading region; at least one corresponding vesicle-capture material, wherein said vesicle-capture material includes glass-like materials; and at least one corresponding sample receiving region, wherein passage of the biological fluid from the sample loading region through the vesicle-capture material and into the sample receiving region results in the capture of vesicles. Additional embodiments provide for a method of isolating vesicles and vesicle-like materials from biological fluids.
Type:
Grant
Filed:
January 7, 2015
Date of Patent:
October 17, 2017
Assignees:
HITACHI CHEMICAL CO., LTD., HITACHI CHEMICAL COMPANY AMERICA, LTD.
Abstract: Provided is a device that collects vesicles and vesicle-like materials from biological fluids. Such devices comprise at least one sample loading region; at least one corresponding vesicle-capture material, wherein said vesicle-capture material comprises glass-like materials; and at least one corresponding sample receiving region, wherein passage of the biological fluid from the sample loading region through the vesicle capture material and into the sample receiving region results in the capture of vesicles. Additional methods provide for a method of isolating vesicles and vesicle-like materials from biological fluids are also provided.
Type:
Grant
Filed:
June 10, 2011
Date of Patent:
August 1, 2017
Assignees:
HITACHI CHEMICAL CO., LTD., HITACHI CHEMICAL COMPANY AMERICA, LTD.
Abstract: Provided herein are devices and methods for the capture or isolation of a biomarker from a biological sample. In several embodiments, the device comprises a loading region, a filter material, and a receiving region. In particular, in several embodiments, biological fluid is passed from the loading region through the filter material and into the receiving region, thereby resulting in capture or isolation of a biomarker.
Type:
Grant
Filed:
October 2, 2013
Date of Patent:
May 30, 2017
Assignees:
HITACHI CHEMICAL COMPANY AMERICA, LTD., HITACHI CHEMICAL COMPANY LTD.
Abstract: Provided herein are devices and methods for the capture or isolation of a biomarker from a biological sample. In several embodiments, the device comprises a loading region, a filter material, and a receiving region. In particular, in several embodiments, biological fluid is passed from the loading region through the filter material and into the receiving region, thereby resulting in capture or isolation of a biomarker.
Type:
Grant
Filed:
October 2, 2013
Date of Patent:
March 21, 2017
Assignees:
HITACHI CHEMICAL COMPANY AMERICA, LTD., HITACHI CHEMICAL COMPANY LTD.
Abstract: Embodiments of the invention relate generally to ex vivo methods of quantifying expression of interferon responsive genes and characterizing an individual's potential responsiveness to interferon administration. Certain embodiments relate to methods to monitor the efficacy of ongoing interferon therapy by evaluating expression of interferon responsive genes before and after interferon administration.
Type:
Grant
Filed:
May 3, 2011
Date of Patent:
October 6, 2015
Assignees:
HITACHI CHEMICAL CO., LTD., HITACHI CHEMICAL COMPANY AMERICA, LTD, THE CLEVELAND CLINIC FOUNDATION