Patents Assigned to Johnson & Johnson Clinical Diagnostics, Inc.
  • Patent number: 5811253
    Abstract: Aqueous compositions, test kits and methods can be used to detect hydrogen peroxide or vanadium bromoperoxidase by generating a chemiluminescent signal in the presence of the analyte. Signal generation as well as reaction kinetics are improved by using a composition which comprises a 2,3-dihydro-1,4-phthalazinedione derivative; a halogen, pseudohalogen, halogen-providing or pseudohalogen-providing source; and a peroxide or peroxide-generating reagent composition.
    Type: Grant
    Filed: September 1, 1995
    Date of Patent: September 22, 1998
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: Alan Eric Friedman, Sarah Fingar Groulx, Alison Butler
  • Patent number: 5776714
    Abstract: Modified apo-peroxidases have been found useful in analytical methods to remove the bias caused by the presence of hemoglobin in the test specimens. While apo-peroxidases are known to remove serum interferents, they fail to overcome the hemoglobin bias, particularly when dry coated analytical elements are used in the assays. Blocking the nitrogen atoms of the imidzolyl groups of the histidine amino acids of the apo-peroxidase prevents catalytic activity of the protein in the presence of hemoglobin.
    Type: Grant
    Filed: October 10, 1995
    Date of Patent: July 7, 1998
    Assignee: Johnson & Johnson Clinical Diagnostics Inc.
    Inventor: Roy Eugene Snoke
  • Patent number: 5772584
    Abstract: An apparatus capable of receiving measurements relating to a fetus carried by a pregnant woman and computer means for comparing these measurements to reference data to determine fetal abnormalities. The measurements relate to the difference between the gestational age of the woman, as determined by reference to the menstrual period, and biometric measurement of the fetus. The apparatus can be used for antenatal screening for chromosomal abnormalities.
    Type: Grant
    Filed: August 7, 1995
    Date of Patent: June 30, 1998
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventor: Christopher John Davies
  • Patent number: 5753512
    Abstract: Method and apparatus for determining actual liquid volumes in vessels in an incubator rotor that experiences vertical run-out as it rotates past stations that fill the vessels with liquid. Air pressure is used to determine the height of the bottom of each of the empty vessels, and then the height of the liquid that fills the vessels, so that the difference in height is converted into volume to compare with the desired and expected volume for the operation that does the volume-filling.
    Type: Grant
    Filed: November 13, 1996
    Date of Patent: May 19, 1998
    Assignee: Johnson & Johnson Clinical Diagnostics, inc
    Inventors: James Daniel Riall, David Donald Hyde
  • Patent number: 5747345
    Abstract: Diaza-18-crown-6 compounds of the formula I: ##STR1## wherein X is a phenol, naphthol or quinolinol moiety selected from those of the formulae: ##STR2## R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are independently selected from hydrogen, halo, cyano, nitro, (C.sub.1 -C.sub.6) alkyl, (C.sub.2 -C.sub.8) acyl, acetamido, mercapto, alkyl or arylsulfonyl, trifluoromethyl, aryl, and substituted aryl wherein the aryl moiety is selected from phenyl and naphthyl and the aryl substituent is selected from halo, cyano, nitro, (C.sub.1 -C.sub.6) alkyl, (C.sub.2 -C.sub.8) acyl, phenyl, acetamido, mercapto, alkyl or arylsulfonyl and trifluoromethyl; provided that when R.sub.1, R.sub.2, R.sub.3 and R.sub.5 are hydrogen and X is phenol, R.sub.4 is not nitro; and provided that when X is phenol and R.sub.5 is hydrogen, R.sub.1 (or R.sub.2) and R.sub.4 are not both nitro.
    Type: Grant
    Filed: August 15, 1996
    Date of Patent: May 5, 1998
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: Wayne Woodrow Weber, II, Calvin Roman Messing, Margaret Elizabeth Logan
  • Patent number: 5736403
    Abstract: A method for determining and correcting for the variations in height of a rotor as it wobbles when rotating past a particular critical circumferential position. The method comprises sensing a reference surface of each vessel in the rotor that is to be filled during the use of the rotor in an incubator, as a tare height of the vessel, so that the effect of the vertical run-out is known and corrected for by the computer. Air pressure is used to detect the reference surface, either from a vessel-wash probe connected to the source of air pressure, or a separate sensor probe.
    Type: Grant
    Filed: November 13, 1996
    Date of Patent: April 7, 1998
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: James Daniel Riall, David Donald Hyde
  • Patent number: 5715611
    Abstract: Humidity control apparatus for articles being stored, comprising: a) a chamber within which the articles are to be contained, b) a conveyor passing by a portion of, and exposed to, the contents of the chamber, c) a drive mechanism for driving the conveyor past the portion of the chamber, d) a desiccant on the conveyor, e) a humidistat positioned to measure the water vapor content within the chamber, and f) a control mechanism operatively connecting the humidistat and the drive mechanism for advancing fresh desiccant past the chamber when the water vapor content of the chamber is too high.
    Type: Grant
    Filed: October 18, 1996
    Date of Patent: February 10, 1998
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: Merrit Nyles Jacobs, Roger G. Leighton, John J. Meyers
  • Patent number: 5714340
    Abstract: A dry immunoassay analytical element, for assaying a ligand is disclosed. The element comprises, in the following order, (a) a layer containing a labeled ligand, (b) a bead spreading layer, c) a cross-linked hydrophilic polymer layer and d) a support; wherein(i) a fixed concentration of an immobilized receptor for the labeled ligand is located in a zone at the interface of layers (b) and (c); and(ii) the receptors are immobilized by being covalently bonded to polymeric beads that are smaller than the beads in layer (c).
    Type: Grant
    Filed: June 16, 1994
    Date of Patent: February 3, 1998
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: Richard Calvin Sutton, Margaret Jeanette Smith-Lewis, Linda Ann Mauck, Wayne Arthur Bowman, Susan Jean Danielson
  • Patent number: 5708278
    Abstract: A reflective wetting or spot detecting system incorporates a light emitting diode and a solid state photo sensor arranged on the same side of a medium being analyzed. The sensor is carried at one end of an elongated housing with a linear channel extending through. The channel is oriented on a line that is normal to the medium being analyzed. The channel functions as a lensless, passive focuser of reflected radiant energy. The source of radiant energy is located at an acute angle with respect to the medium. A conditioning circuit is coupled to the output of the sensor. The conditioning circuitry includes a current-to-voltage converter, a signal reducing stage, an amplifying stage and a comparator. The signal reducing stage reduces the voltage signal, produced by the current voltage converter, a predetermined amount, thereby effectively providing the amplifier stage with a greater dynamic range than it would have otherwise had.
    Type: Grant
    Filed: May 13, 1996
    Date of Patent: January 13, 1998
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventor: Alan John Lowne
  • Patent number: 5705357
    Abstract: A simplified composition for generating a chemiluminescent signal in the presence of a peroxidase includes a substituted acetanilide as the sole light-producing substrate for the peroxidase. Assays for various analytes can be carried out with this composition at near neutral pH, exhibit low background and are highly sensitive.
    Type: Grant
    Filed: August 29, 1994
    Date of Patent: January 6, 1998
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: Thomas Robert Kissel, Alan E. Friedman, Sarah A. Fingar
  • Patent number: 5705366
    Abstract: Multiple target nucleic acids are amplified using polymerase chain reaction in the presence of a nonionic, polymeric volume exclusion agent. The amplification efficiency of low copy target nucleic acids is increased in the presence of the volume exclusion agent even though reduced primer levels are used. In this manner, amplification efficiency of a given target nucleic acid can be more readily manipulated.
    Type: Grant
    Filed: September 15, 1994
    Date of Patent: January 6, 1998
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventor: John W. Backus
  • Patent number: 5702884
    Abstract: Leucocytes can be quickly and selectively separated from erythrocytes by subjecting a whole blood sample to a series of steps including lysing the erythrocytes and washing the remaining leucocytes with a solution containing ammonium chloride and a carboxylic acid or a metal carboxylate. The resulting white blood cells can be readily lysed and subjected to polymerase chain reaction to amplify and detect a target nucleic acid. The test kit useful in practicing the amplification method includes a labeled primer, a PCR reagent and a reagent mixture containing ammonium chloride and a carboxylic acid or a metal carboxylate for sample preparation.
    Type: Grant
    Filed: March 12, 1996
    Date of Patent: December 30, 1997
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: Tobias E. Ekeze, JoAnne Hansen Kerschner
  • Patent number: 5686254
    Abstract: A dry immunoassay analytical element for assaying a ligand, comprising a support bearing:1. an enzyme labeled ligand or an enzyme labeled receptor zone;2. a spreading zone; and3. a receptor zone containing a fixed concentration of an immobilized receptor for the ligand and the labeled ligand when present and the receptor is covalently bonded to polymeric beads having a diameter in the range of 0.1 to 5 .mu.m; characterized in that the element contains a diaryl telluride (DAT) compound and the zones can be in the same or separate layers.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: November 11, 1997
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: Margaret Elizabeth Logan, Carol Anne DeCann, Marsha Denise Bale Oenick, Gary Louis Snodgrass, Roy Eugene Snoke
  • Patent number: 5674717
    Abstract: Nucleic acids can be amplified and detected using a very rapid polymerase chain reaction procedure in which two different nucleic acid sequences are present. This method allows one to preferentially modulate (for example, suppress) the degree of amplification of one or more nucleic acid sequences relative to other nucleic acid sequences. This modulation is achieved by exploiting differences in the relative primer melt temperatures, or by using certain ratios of primers. Each PCR cycle is very fast, that is less than about 90 seconds. This method is particularly useful for amplification and detection of DNA associated with infectious agents that may be present in a specimen in very small quantities compared to other nontargeted nucleic acids.
    Type: Grant
    Filed: October 25, 1995
    Date of Patent: October 7, 1997
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: John W. Backus, William Harold Donish, John Bruce Findlay, John William H. Sutherland, Marlene M. King
  • Patent number: 5672512
    Abstract: A chain incubator or conveyor, and method of use in an analyzer, wherein an endless chain is formed of repeating members and each comprising a link, element support, a cover, and means for biasing the cover onto the support with an article such as a test element between them. The members are linked together by a pair of pivot pins, and the chain is engaged by a drive sprocket and an idler sprocket.
    Type: Grant
    Filed: February 12, 1996
    Date of Patent: September 30, 1997
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventor: James D. Shaw
  • Patent number: 5665601
    Abstract: An aspirator/dispenser of liquid has a sensor for sensing contact with the liquid by the dispense vessel, which uses constant pressure air expelled out the tip of the dispense vessel. The sensor is improved in that the line of air pressure feeding into the vessel is vented in parallel with the vessel, to the atmosphere, so that the spike of pressure occurring when the vessel encounters the liquid, is released out the vent, rather than the vessel, thus avoiding bubble formation in the liquid.
    Type: Grant
    Filed: January 22, 1996
    Date of Patent: September 9, 1997
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventor: Kathleen Ellen Kilmer
  • Patent number: 5654416
    Abstract: An aqueous composition containing primers for opposing strands of two or more target nucleic acids can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a T.sub.m within the range of from about 65.degree. to about 74.degree. C., while the T.sub.m 's are within about 5.degree. C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of multiple nucleic acids, or in "multiplexing", using multiple capture probes. All of the capture probes have T.sub.m 's which are greater than 50.degree. C. and are within 15.degree. C. of each other.
    Type: Grant
    Filed: June 8, 1995
    Date of Patent: August 5, 1997
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: Thomas J. Cummins, Susan Melissa Atwood, Lynn Bergmeyer, John Bruce Findlay, John W. H. Sutherland, JoAnne H. Kerschner
  • Patent number: 5652346
    Abstract: What is disclosed is a dicarboxylic acid oxidation product of a immunologically reactive mono- or a polysaccharide having vicinal diols.
    Type: Grant
    Filed: March 6, 1996
    Date of Patent: July 29, 1997
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: Michael Ray Detty, Susan Jean Danielson
  • Patent number: 5650324
    Abstract: Monoclonal antibodies have been prepared which are of the IgG isotype and are highly specific for horseradish peroxidase. One group of antibodies inhibits at least about 95% of the normal activity of horseradish peroxidase when bound to the enzyme. A second group of antibodies inhibits less than about 20% of the enzymatic activity when bound to the enzyme, but prevents the binding of the antibodies from the first group. The antibodies in either group can be conjugated to specific binding ligands such as drugs or hormones.
    Type: Grant
    Filed: May 31, 1994
    Date of Patent: July 22, 1997
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventors: Kevin Matthew Gorman, John Linforth Daiss
  • Patent number: 5641688
    Abstract: A method of separating bound labeled indicator from free labeled indicator in a layer of a test element for immunoassay. The method comprisesa) depositing sample containing a target immunoanalyte capable of binding to the labeled indicator or to an immobilized antibody in competition with the labeled indicator, onto an exterior surface of a test element in the presence of the labeled indicator andb) adding an amount of wash liquid to the exterior surface to form a pool of the liquid having a meniscus on the surface, the liquid penetrating the surface over an area bounded by a closed intersect edge formed between the pool meniscus and the surface, so that penetrating liquid can push free labeled indicator away from bound labeled indicator in a volume of the layer below the bounded area.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: June 24, 1997
    Assignee: Johnson & Johnson Clinical Diagnostics, Inc.
    Inventor: Merrit Nyles Jacobs