Patents Assigned to Johnson & Johnson Research Pty. Limited
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Publication number: 20130344040Abstract: Described are compositions and methods relating to gene therapy, particularly as applied to hematopoietic progenitor (HP) cells, to transduced cells and methods of obtaining them, and to methods of using them to provide prolonged engraftment of modified hematopoietic cells in human subjects. The invention particularly relates to ex vivo gene therapy of HP cells for treatment or prevention of HIV infection.Type: ApplicationFiled: July 8, 2013Publication date: December 26, 2013Applicant: JOHNSON & JOHNSON RESEARCH PTY. LIMITEDInventors: Geoffrey P. Symonds, Rafael Amado, Lun-Quan Sun, Janet Macpherson, Greg Fanning, Wayne Gerlach
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Patent number: 8097715Abstract: Interfering RNA molecules are now designed and produced with specificity for multiple binding sequences present in distinct genetic contexts in one or more pre-selected target RNA molecules and are used to modulate expression of the target sequences. The multitargeting interfering RNA molecules have two strands that target multiple target sites on one or more pre-selected RNA molecules. Such a multitargeting interfering RNA approach provides a powerful tool for gene regulation.Type: GrantFiled: May 21, 2008Date of Patent: January 17, 2012Assignee: Johnson & Johnson Research PTY LimitedInventors: Laurent Pierre Rivory, Michael Poidinger, Donald John Birkett, Gregory Martin Arndt, Toby Passioura
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Patent number: 7994144Abstract: Described are compositions and methods relating to gene therapy, particularly as applied to hematopoietic progenitor (HP) cells, to transduced cells and methods of obtaining them, and to methods of using them to provide prolonged engraftment of modified hematopoietic cells in human subjects. The invention particularly relates to ex vivo gene therapy of HP cells for treatment or prevention of HIV infection.Type: GrantFiled: July 10, 2002Date of Patent: August 9, 2011Assignee: Johnson & Johnson Research Pty, LimitedInventors: Geoffrey P. Symonds, Rafael Amado, Lun-Quan Sun, Janet Macpherson, Greg Fanning, Wayne Gerlach
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Publication number: 20110143338Abstract: The present invention relates to methods that utilise multi-component nucleic acid complex (MNA complex) cascades. The MNA complexes may have cleavage or ligase activity. Further, the invention provides cascades which may include one or more DNAzymes. The invention also provides methods which use these cascades for the identification, detection and quantification of targets.Type: ApplicationFiled: April 4, 2008Publication date: June 16, 2011Applicant: Johnson & Johnson Research Pty LimitedInventors: Alison Velyian Todd, Elisa Mokany, Tram Bich Doan, Paul Ean Young, Helen Ann Swain
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Publication number: 20100305186Abstract: The present invention is concerned with methods for enhancing gene suppression in cells and in particular it is concerned with improved methods for enhancing RNAi-mediated gene silencing by manipulation of factors associated with RNAi. The present invention is also concerned with methods for identifying factors which down-regulate as well as those which up-regulate RNAi. It is also concerned with genetic constructs useful for enhancing or modulating gene silencing and cells harbouring such constructs.Type: ApplicationFiled: May 29, 2001Publication date: December 2, 2010Applicant: JOHNSON & JOHNSON RESEARCH PTY LIMITEDInventors: Gregory Martin Arndt, Mitch Raponi
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Publication number: 20100221711Abstract: The invention concerns a method for the extraction of nucleic acids from biological samples e.g. tissue material or sputum derived from human or animal species and the quantitative detection thereafter of said nucleic acids e.g. in terms of viral load, more specifically RSV viral load detection.Type: ApplicationFiled: April 4, 2008Publication date: September 2, 2010Applicant: Johnson & Johnson Research PTY LimitedInventors: David Nauwelaers, Lieven Jozef Stuyver, Alison Velyian Todd, Elisa Mokany, Paul Ean Young
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Patent number: 7776595Abstract: Described are compositions and methods relating to gene therapy, particularly as applied to hematopoietic progenitor (HP) cells, to transduced cells and methods of obtaining them, and to methods of using them to provide prolonged engraftment of modified hematopoietic cells in human subjects. The invention particularly relates to ex vivo gene therapy of HP cells for treatment or prevention of HIV infection.Type: GrantFiled: August 18, 2006Date of Patent: August 17, 2010Assignee: Johnson & Johnson Research Pty, LimitedInventors: Geoffrey P. Symonds, Rafael G. Amado, Lun-Quan Sun, Janet L. MacPherson, Gregory C. Fanning, Wayne Gerlach
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Publication number: 20100136536Abstract: The present invention relates to compositions and methods that allow the manipulation of the catalytic activity of multi-component nucleic acid complexes. Further, the invention provides methods which use these compositions and methods to create molecular sensors, molecular switches, and/or modulators or propagators of autocatalytic self-replicating cascades and other iterative processes. More particularly, the invention relates to compositions allowing self-assembly of active and inactive multicomponent nucleic acid complexes, methods of making such compositions, and methods for use.Type: ApplicationFiled: October 5, 2007Publication date: June 3, 2010Applicant: Johnson & Johnson Research Pty. LimitedInventors: Alison V. Todd, Elisa Mokany, Donald J. Birkett, Tram Bich Doan, Christopher R. Reid
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Publication number: 20090239816Abstract: Interfering RNA molecules are now designed and produced with specificity for multiple binding sequences present in distinct genetic contexts in one or more pre-selected target RNA molecules and are used to modulate expression of the target sequences. Such a multitargeting interfering RNA approach provides a powerful tool for gene regulation.Type: ApplicationFiled: May 21, 2008Publication date: September 24, 2009Applicant: Johnson & Johnson Research PTY. LimitedInventors: Laurent Pierre Rivory, Michael Poidinger, Donald John Birkett, Gregory Martin Arndt, Toby Passioura
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Publication number: 20090192103Abstract: Interfering RNA molecules are now designed and produced with specificity for multiple binding sequences present in distinct genetic contexts in one or more pre-selected target RNA molecules and are used to modulate expression of the target sequences. The multitargeting interfering RNA molecules have two strands that target multiple target sites on one or more pre-selected RNA molecules. Such a multitargeting interfering RNA approach provides a powerful tool for gene regulation.Type: ApplicationFiled: May 21, 2008Publication date: July 30, 2009Applicant: Johnson & Johnson Research PTY. LimitedInventors: Laurent Pierre RIVORY, Michael Poidinger, Donald John Birkett, Gregory Martin Arndt, Toby Passioura
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Patent number: 7345025Abstract: Described are compositions and methods relating to gene therapy, particularly as applied to hematopoietic progenitor (HP) cells, to transduced cells and methods of obtaining them, and to methods of using them to provide prolonged engraftment of modified hematopoietic cells in human subjects. The invention particularly relates to ex vivo gene therapy of HP cells for treatment or prevention of HIV infection.Type: GrantFiled: July 10, 2002Date of Patent: March 18, 2008Assignee: Johnson & Johnson Research Pty. LimitedInventors: Geoffrey P. Symonds, Rafael G. Amado, Lun-Quan Sun, Janet L. MacPherson, Gregory C. Fanning, Wayne Gerlach
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Patent number: 7193127Abstract: The present invention concerns codeinone reductase from alkaloid poppy plants, the polynucleotides encoding the enzyme, transgenic plants transformed or transfected with polynucleotide(s) encoding codeinone reductase and to the production of alkaloids from transformed or transfected poppy plants.Type: GrantFiled: March 24, 2000Date of Patent: March 20, 2007Assignee: Johnson & Johnson Research Pty. LimitedInventors: Toni M. Kutchan, Meinhard H. Zenk, David G. Atkins, Anthony J. Fist
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Publication number: 20060240462Abstract: Provided herein are methods for the combined amplification and detection of one or a plurality of target nucleic acid molecules. The methods encompass the use of an antisense strand of a catalytic nucleic acid in a primer for amplification such that an amplicon produced thereby includes an active catalytic nucleic acid capable of indicating the presence of the target sequences through the modification of a reporter substrate. Devices and kits are also provided. DNA molecules for practicing the methods are also provided. The DNA molecules comprise at least a first portion complementary to at least a first portion of a target nucleic acid sequence, a second portion complementary to an antisense sequence of a second portion of the target nucleic acid sequence, and a third portion comprising an antisense sequence of a catalytic nucleic acid; the third portion positioned between the first and second portions of said DNA molecule.Type: ApplicationFiled: April 21, 2006Publication date: October 26, 2006Applicant: Johnson & Johnson Research Pty LimitedInventors: Alison Todd, Tanya Applegate, Tram Doan, Paul Young
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Patent number: 7037674Abstract: The present invention relates to production of alkaloids from poppy plants and in particular togenes encoding enzymes in the alkaloid pathway, to proteins encoded by the gens, to plants transformed or transfected with the genes and to methods of altering alkaloid content or blend of poppy plants.Type: GrantFiled: August 28, 1998Date of Patent: May 2, 2006Assignee: Johnson & Johnson Research PTY LimitedInventors: Toni M. Kutchan, Meinhart H. Zenk, David G. Atkins, Anthony J. Fist
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Patent number: 6361941Abstract: This invention provides methods and kits useful for determining whether a subject is afflicted with a disorder characterized by the presence of one or more known nucleic acid mutations. The instant methods comprise steps of nucleic acid molecule isolation, amplification, contact with one or more catalytic nucleic acid molecules specifically cleaving a target sequence present either in the case of disorder or wild-type, but not both, and determining cleavage of the amplified segment(s).Type: GrantFiled: March 16, 1999Date of Patent: March 26, 2002Assignee: Johnson & Johnson Research Pty LimitedInventors: Alison V. Todd, Caroline J. Fuery, Murray J. Cairns
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Patent number: 6261768Abstract: The present invention provides a method of detecting a genetic polymorphism in an individual. In one form, the method comprises the following steps: (1) obtaining a sample containing a nucleic acid from the individual; (2) amplifying the nucleic acid sample from step (1) by a process involving thermocycling and primers, the amplification occurring in the presence of a thermostable restriction endonuclease which retains activity during thermocycling, the primers being selected such that they introduce into either the nucleic acid amplified from nucleic acid not including the polymorphism or from nucleic acid including the polymorphism, a sequence recognized by the thermostable restriction endonuclease; and (3) analyzing the product of step (2) to determine the presence of the polymorphism.Type: GrantFiled: January 16, 1998Date of Patent: July 17, 2001Assignee: Johnson & Johnson Research Pty. LimitedInventors: Alison Velyian Todd, Caroline Jane Fuery
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Patent number: 6245505Abstract: The present invention provides a method for amplifying a specific target nucleic acid sequence. The method comprises (1) forming a reaction mixture comprising: (i) the target sequence; (ii) primers comprising a first primer at least a portion of which at the 3′ end thereof is substantially complementary to a first segment at a first end of the target sequence, a second primer at least a portion of which at the 5′ end thereof is substantially complementary to a second segment at a second end of the target sequence, the 5′ end of the second primer being adjacent the 3′ end of the first primer, and a third primer, the third primer being substantially complementary to a segment of the second primer at the 3′ end thereof; (iii) at least four different nucleotide bases; (iv) thermostable polymerase and thermostable ligase; and (2) thermocycling the reaction mixture.Type: GrantFiled: December 17, 1999Date of Patent: June 12, 2001Assignee: Johnson & Johnson Research Pty. LimitedInventors: Alison Velyain Todd, Caroline Jane Fuery
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Patent number: 6140055Abstract: This application provides methods of detecting and quantitatively determining a target nucleic acid sequence in a sample, which comprise contacting the sample with a primer and a zymogene which encodes, but which itself is the anti-sense sequence of, a catalytic nucleic acid sequence, so that when the target is present, a single amplified nucleic acid molecule is produced which comprises the sequences of both the target and catalytic molecules. This invention further provides a method of simultaneously detecting the presence of a plurality of target nucleic acid sequences in a sample. Finally, this invention provides molecules and kits for practicing the instant methods.Type: GrantFiled: February 22, 1999Date of Patent: October 31, 2000Assignee: Johnson & Johnson Research Pty LimitedInventors: Alison V. Todd, Caroline J. Fuery, Murray J. Cairns