Patents Assigned to Life Technologies Corporation
  • Publication number: 20230307095
    Abstract: A method for nucleic acid sequencing includes receiving nucleic acid sequencing data from a sequencing instrument that receives and processes a sample nucleic acid in a sequencing-by-synthesis process. The method also includes generating a set of candidate sequences of bases for the observed or measured nucleic acid sequencing data by determining a predicted signal for candidate sequences using a simulation framework. The simulation framework incorporates an estimated carry forward rate (CFR), an estimated incomplete extension rate (IER), an estimated droop rate (DR), an estimated reactivated molecules rate (RMR), and an estimated termination failure rate (TFR), the RMR being greater than or equal to zero and the TFR being lesser than one. The method also includes identifying, from the set of candidate sequences of bases, a candidate sequence as corresponding to the sequence for the sample nucleic acid.
    Type: Application
    Filed: March 28, 2023
    Publication date: September 28, 2023
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Christian KOLLER, Marcin SIKORA, Peter VANDER HORN
  • Publication number: 20230227919
    Abstract: The disclosure provides gene fusions, gene variants, and novel associations with disease states, as well as kits, probes, and methods of using the same.
    Type: Application
    Filed: January 24, 2023
    Publication date: July 20, 2023
    Applicant: Life Technologies Corporation
    Inventors: Daniel Rhodes, Seth Sadis, Peter Wyngaard, Nikolay Khazanov, Santhoshi Bandla, Mark Tomilo, Sean Eddy, Emma Bowden, Jia Li
  • Publication number: 20230223103
    Abstract: Methods and systems for improving computer efficiency by intelligently selecting subsets of possible short tandem repeat (STR) allele combinations for further deconvolution analysis are disclosed. In one embodiment, at each locus, for a currently analyzed contribution ratio scenario of a plurality of contribution ratio scenarios, a processor computes an adjusted evidence profile. For a first, or next, unidentified contributor having a pre-determined highest remaining contribution ratio in the currently analyzed contribution ratio scenario for the plurality of contributors, a processor computes a first range of expected peak heights using at least the pre-determined highest remaining contribution ratio, a selected degradation value, and a peak height ratio distribution. Also disclosed are methods and systems for intelligently estimating the number of contributors to a biological sample.
    Type: Application
    Filed: December 28, 2022
    Publication date: July 13, 2023
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventor: Chantal Roth
  • Patent number: 11685886
    Abstract: A method for filtering a gas includes delivering a gas into a compartment of a gas filter assembly; applying a partial vacuum to the gas filter assembly so that the partial vacuum assists in drawing the gas through a porous filter body of the gas filter assembly that is at least partially disposed within the compartment of the gas filter assembly; and regulating the application of the partial vacuum based on a pressure reading of the gas upstream or downstream of the gas filter assembly.
    Type: Grant
    Filed: October 29, 2020
    Date of Patent: June 27, 2023
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Nephi D. Jones, Christopher D. Brau
  • Patent number: 11679924
    Abstract: A tube holder assembly includes a base plate, an attachment plate, and an optional securing element. The base plate may include guides into which protrusions of the attachment plate slide to alter the positioning apparatus from an open position to a closed position. The base plate also includes receivers to receive tubular members, which have their movement restrained by the receivers and the securing elements in the closed position. The tube holder assembly may also include a latch to help maintain the closed position during operation.
    Type: Grant
    Filed: June 29, 2022
    Date of Patent: June 20, 2023
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Tristan Wise, Charles Rhodes
  • Patent number: 11680905
    Abstract: A biological analysis system can include an excitation module and an emission module. The excitation module can include a collimator element for receiving excitation light from the excitation light source and transmitting collimated excitation light in a first direction, and a plurality of excitation mirrors arrayed along the excitation light path, each excitation mirror disposed at an acute angle relative to the first direction and configured to reflect collimated excitation light along a second direction. The emission module can be positioned to receive excitation light transmitted along the second direction and can include a sample block comprising a plurality of sample receptacles positioned to receive a beam of collimated excitation light, and a plurality of photodetectors configured to receive emission light transmitted from a respective sample receptacle in a direction transverse to the second direction of the excitation light path.
    Type: Grant
    Filed: January 11, 2022
    Date of Patent: June 20, 2023
    Assignees: Life Technologies Holdings PTE LTD, Life Technologies Corporation
    Inventors: Ming Song Chen, Chee Woei Chong, Wuh Ken Loh, Wern Yuh Foo, Kuan Moon Boo, Norbert Leclerc, Kristina Uhlendorf, Reiner Koellner, Torsten Fuchs, Stefan Breitfelder
  • Patent number: 11661629
    Abstract: Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.
    Type: Grant
    Filed: October 28, 2019
    Date of Patent: May 30, 2023
    Assignee: Life Technologies Corporation
    Inventors: Stephen Hendricks, David J. King
  • Patent number: 11664090
    Abstract: A method of automatically sequencing or basecalling one or more DNA (deoxyribonucleic acid) molecules of a biological sample is described. The method comprises using a capillary electrophoresis genetic analyzer to measure the biological sample to obtain at least one input trace comprising digital data corresponding to fluorescence values for a plurality of scans. Scan labelling probabilities for the plurality of scans are generated using a trained artificial neural network comprising a plurality of layers including convolutional layers. A basecall sequence comprising a plurality of basecalls for the one or more DNA molecules based on the scan labelling probabilities for the plurality of scans is determined.
    Type: Grant
    Filed: June 11, 2020
    Date of Patent: May 30, 2023
    Assignee: Life Technologies Corporation
    Inventors: Yong Chu, Stephanie Jo Schneider, Rylan Schaeffer, David Woo
  • Patent number: 11655500
    Abstract: A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.
    Type: Grant
    Filed: June 3, 2019
    Date of Patent: May 23, 2023
    Assignee: Life Technologies Corporation
    Inventors: Earl Hubbell, Christian Koller, Nils Homer
  • Patent number: 11657893
    Abstract: A method of modeling a background signal when sequencing a polynucleotide strand using sequencing-by-synthesis includes: flowing a series of nucleotide flows onto a reactor array having multiple reaction confinement regions, one or more copies of the polynucleotide strand being located in a loaded reaction confinement region of the reactor array, the loaded reaction confinement region being located in a vicinity of one or more neighboring reaction confinement regions that may or may not be loaded; receiving output signals from the reactor array; and modeling a background signal for the loaded reaction confinement region using the received output signals and a model adapted to account at least for an exchange of ions between the one or more neighboring reaction confinement regions and a headspace adjacent the loaded reaction confinement region and the one or more neighboring reaction confinement regions.
    Type: Grant
    Filed: May 11, 2020
    Date of Patent: May 23, 2023
    Assignee: Life Technologies Corporation
    Inventor: Todd Rearick
  • Patent number: 11654408
    Abstract: A method for operating a bioreactor includes passing a drive shaft into a first tubular connector and a second tubular connector projecting from the first tubular connector, the first tubular connector and the second tubular connector being at least partially disposed within a container, the first tubular connector being more flexible than the second tubular connector, the second tubular connector having a length that comprises at least 20% of a combined length of the first tubular connector and the second tubular connector. Rotating the drive shaft so as to rotate the first tubular connector and the second tubular connector within the container.
    Type: Grant
    Filed: May 23, 2019
    Date of Patent: May 23, 2023
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventor: Trevor G. Williams
  • Patent number: 11649479
    Abstract: Provided herein are compositions, methods and uses that relate to an easy, accurate and reliable dual or duplex assay that determines both protein and nucleic acid amounts in a viral preparation in a single container and further determines full versus empty virion content.
    Type: Grant
    Filed: March 22, 2019
    Date of Patent: May 16, 2023
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Yixin Li, Bharti Solanki, Sheung-Mei Shih, Peter Bell, Darick Dayne
  • Publication number: 20230134044
    Abstract: A thermal cycler system comprises a sample block configured to receive a sample holder configured to receive a plurality of samples; an adaptor configured to surround a periphery of the sample block; and a drip pan configured to surround a periphery of the adaptor. The drip pan comprises one or more ejector mechanisms and one or more openings, the one or more ejector mechanisms configured to respectively extend through the one or more openings into contact with the sample holder in a state of the sample holder received by the sample block and the adaptor surrounding the periphery of the sample block.
    Type: Application
    Filed: December 29, 2022
    Publication date: May 4, 2023
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Zeqi TAN, Wuh Ken LOH, Siew Yin LEE, Kuan Moon (Bernard) BOO
  • Publication number: 20230133734
    Abstract: A system for nucleic acid sequencing includes a machine-readable memory and a processor configured to execute machine-readable instructions. The instructions, when executed by the processor, cause the system to expose template polynucleotide strands in a plurality of defined spaces of a sensor array to a series of flows of nucleotide species, the series comprising a sequence of random flows; and obtain, for each of the series of flows of nucleotide species, a signal indicative of how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands.
    Type: Application
    Filed: January 23, 2023
    Publication date: May 4, 2023
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Earl HUBBELL, Jonathan SCHULTZ
  • Patent number: 11639240
    Abstract: A fluid manifold system includes a first manifold having portions of opposing flexible sheets welded together to form a fluid flow path therebetween, a fluid inlet communicating with the fluid flow path. The fluid flow path of the first manifold includes: a primary flow path communicating with the fluid inlet of the first manifold; and a plurality of spaced apart secondary flow paths that branch off of the primary flow path. Each secondary flow path has a first end communicating with the primary flow path and an opposing second end, each secondary flow path having a diameter that is smaller than a diameter of the primary flow path. The fluid manifold system also includes a plurality of tubular connectors with each tubular connector being secured to the first manifold at the second end of a corresponding one of the secondary flow paths.
    Type: Grant
    Filed: September 22, 2021
    Date of Patent: May 2, 2023
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Michael E. Goodwin, Jeremy K. Larsen, Patrick L. Draper, Brandon M. Knudsen
  • Publication number: 20230127610
    Abstract: A computer-implemented method for generating a data visualization for operating a digital polymerase chain reaction (dPCR) system comprises, the method comprising receiving fluorescent emission data comprising a plurality of data points corresponding to fluorescence emission from a plurality of reaction sites of a substrate, the fluorescent emission data indicative of presence or absence of amplification product of a dPCR. The method further comprises displaying a data visualization of the plurality of data points in a spatial representation of the substrate such that each data point is displayed at a relative spatial location of its corresponding reaction site of the plurality of reaction sites of the substrate, a first set of data points being displayed with a first indication based on meeting a first quality value threshold and second set of data points being displayed with a second indication, differing from the first indication, based on a second quality value threshold.
    Type: Application
    Filed: September 19, 2022
    Publication date: April 27, 2023
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Nivedita Sumi MAJUMDAR, Harrison LEONG, Theodore E. STRAUB, David HOARD
  • Publication number: 20230125333
    Abstract: In one embodiment, a device is described. The device includes a material defining a reaction region. The device also includes a plurality of chemically-sensitive field effect transistors have a common floating gate in communication with the reaction region. The device also includes a circuit to obtain respective output signals from the chemically-sensitive field effect transistors indicating an analyte within the reaction region.
    Type: Application
    Filed: October 7, 2022
    Publication date: April 27, 2023
    Applicant: Life Technologies Corporation
    Inventors: Jonathan M. ROTHBERG, Keith G FIFE, Jordan OWENS, James BUSTILLO
  • Patent number: 11636922
    Abstract: A method for nucleic acid sequencing includes receiving observed or measured nucleic acid sequencing data from a sequencing instrument that receives and processes a sample nucleic acid in a termination sequencing-by-synthesis process. The method also includes generating a set of candidate sequences of bases for the observed or measured nucleic acid sequencing data by determining a predicted signal for candidate sequences using a simulation framework. The simulation framework incorporates an estimated carry forward rate (CFR), an estimated incomplete extension rate (IER), an estimated droop rate (DR), an estimated reactivated molecules rate (RMR), and an estimated termination failure rate (TFR), the RMR being greater than or equal to zero and the TFR being lesser than one. The method also includes identifying, from the set of candidate sequences of bases, one candidate sequence leading to optimization of a solver function as corresponding to the sequence for the sample nucleic acid.
    Type: Grant
    Filed: August 26, 2019
    Date of Patent: April 25, 2023
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Christian Koller, Marcin Sikora, Peter Vander Horn
  • Patent number: 11636919
    Abstract: A method for evaluating variant likelihood includes: providing a plurality of template polynucleotide strands, sequencing primers, and polymerase in a plurality of defined spaces disposed on a sensor array; exposing the plurality of template polynucleotide strands, sequencing primers, and polymerase to a series of flows of nucleotide species according to a predetermined order; obtaining measured values corresponding to an ensemble of sequencing reads for at least some of the template polynucleotide strands in at least one of the defined spaces; and evaluating a likelihood that a variant sequence is present given the measured values corresponding to the ensemble of sequencing reads, the evaluating comprising: determining a measurement confidence value for each read in the ensemble of sequencing reads and modifying at least some model-predicted values using a first bias for forward strands and a second bias for reverse strands.
    Type: Grant
    Filed: May 9, 2018
    Date of Patent: April 25, 2023
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Earl Hubbell, Sowmi Utiramerur
  • Patent number: 11634679
    Abstract: Provided herein is a pipette tip for electroporation including an outer surface, a void located within the outer surface, and a conductor located at least partially on or within the outer surface, in electrical communication with at least a portion of the outer surface and the void. Further provided herein is a pipette tip for electroporation including a body, a connector, and an elongated part. The connector is located at the distal end of the body and further includes at least one connecting post, a connecting part in mechanical communication with the connector, and a conductor located at least partially on or within the connector, wherein the conductor surrounds at least a portion of the connecting post. The elongated part also has a void and is located at the distal end of the connector. The void of the body, connector, and elongated part are in fluid communication.
    Type: Grant
    Filed: September 11, 2019
    Date of Patent: April 25, 2023
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jun-Keun Chang, Keun-Chang Cho, Chan-Il Chung, Neon-Cheol Jung, Seung Gin Huh