Patents Assigned to Life Technologies Corporation
  • Publication number: 20210109060
    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.
    Type: Application
    Filed: October 9, 2020
    Publication date: April 15, 2021
    Applicant: Life Technologies Corporation
    Inventors: Jonathan M. ROTHBERG, Wolfgang HINZ, Kim L. JOHNSON, James BUSTILLO
  • Patent number: 10978174
    Abstract: Methods, system, and kits are provided for sample identification, and, more specifically, for designing, and/or making, and/or using sample discriminating codes or barcodes for identifying sample nucleic acids or other biomolecules or polymers. For example, a plurality of flowspace codewords may be generated, the codewords comprising a string of characters. A location for at least one padding character within the flowspace codewords may be determined. The padding character may be inserted into the flowspace codewords at the determined location. After the inserting, a plurality of the flowspace codewords may be selected based on satisfying a predetermined minimum distance criteria, wherein the selected codewords correspond to valid base space sequences according to a predetermined flow order. And the barcode sequences corresponding to the selected codewords may be manufactured.
    Type: Grant
    Filed: May 13, 2016
    Date of Patent: April 13, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Christian Koller, Michael D. Allen
  • Patent number: 10974247
    Abstract: An interface is provided for storing microfluidic samples in a nanoliter sample chip. A fluid access structure provides a fluid access region to a selected subset of sample wells from an array of sample wells. A fluid introduction mechanism introduces a sample fluid to the fluid access region so that the sample wells in the selected subset are populated with the sample fluid without the unselected sample wells being populated with the sample fluid.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: April 13, 2021
    Assignee: Life Technologies Corporation
    Inventors: Tanya S. Kanigan, Steve Smith, John Linton, Robert Hess, Karl Yoder, Colin J. H. Brenan
  • Patent number: 10976234
    Abstract: The present invention is a method and apparatus for acoustic focusing hardware and implementations.
    Type: Grant
    Filed: February 5, 2018
    Date of Patent: April 13, 2021
    Assignee: Life Technologies Corporation
    Inventors: Gregory Kaduchak, Michael Dennis Ward
  • Publication number: 20210102249
    Abstract: A method for designing test or control sequences may include identifying, using a variant caller, loci with systematic errors present in a plurality of sequencing runs included in a training set of sequencing runs obtained using sequencing-by-synthesis; and selecting a representative set of loci, including selecting from the identified loci an approximately equal number of loci involving errors in A, T, C, and G homopolymers and selecting from the identified loci an approximately equal number of loci involving homopolymers having a length of two, three, and four.
    Type: Application
    Filed: August 20, 2020
    Publication date: April 8, 2021
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventor: Marcin SIKORA
  • Publication number: 20210102916
    Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.
    Type: Application
    Filed: October 1, 2020
    Publication date: April 8, 2021
    Applicant: Life Technologies Corporation
    Inventors: Ronald L. Cicero, Marc Glazer, Yufang Wang, Christina E. Inman, Jeremy Gray, Joseph Koscinski, James A. Ball, Phil Waggoner, Alexander Mastroianni
  • Publication number: 20210102917
    Abstract: An apparatus includes a substrate; a gate structure disposed over the substrate and having an upper surface; a well structure disposed over the substrate and defining a well over the upper surface of the gate structure; a conductive layer disposed on the upper surface of the gate structure and at least partially extending along a wall of the well in the well structure; and a dielectric structure disposed over the well structure and defining an opening to the well.
    Type: Application
    Filed: November 3, 2020
    Publication date: April 8, 2021
    Applicant: Life Technologies Corporation
    Inventors: Jeremy GRAY, Jordan OWENS, James BUSTILLO
  • Patent number: 10968476
    Abstract: A workflow for direct qPCR quantification of unprocessed forensic casework samples is disclosed herein. 13pg of DNA has been detected by direct amplification from a paper substrate. Direct qPCR quantification of unprocessed forensic casework samples and direct STR amplification of unprocessed forensic casework samples collected on the same PE-swab will greatly increase forensic laboratory's efficiency and capability.
    Type: Grant
    Filed: May 3, 2019
    Date of Patent: April 6, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventor: Jason Yingjie Liu
  • Patent number: 10969326
    Abstract: The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.
    Type: Grant
    Filed: January 6, 2020
    Date of Patent: April 6, 2021
    Assignee: Life Technologies Corporation
    Inventors: Gregory Kaduchak, Michael Ward
  • Patent number: 10969325
    Abstract: The present invention comprises methods and systems that use acoustic radiation pressure.
    Type: Grant
    Filed: February 22, 2019
    Date of Patent: April 6, 2021
    Assignee: Life Technologies Corporation
    Inventors: Michael D Ward, Gregory Kaduchak
  • Patent number: 10961571
    Abstract: This specification generally relates to non-radioactive methods of detecting nucleic acid polymerase activity and methods of detecting compounds that modulate nucleic acid polymerase activity. The activity may be measured in real-time using a real-time PCR instrument.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: March 30, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Lei Xi, Keith P. Bjornson, Stephen P. Hendricks
  • Patent number: 10962480
    Abstract: Devices and methods for measuring the quantity of multiple analytes in a sample can include a device designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and machine executable instructions configured to select the proper analyte sensing element corresponding to the analyte to be measured.
    Type: Grant
    Filed: January 7, 2020
    Date of Patent: March 30, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Matthew Beaudet, Jill Hendrickson, David Hagen, Rich Meyer
  • Patent number: 10961528
    Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.
    Type: Grant
    Filed: June 18, 2018
    Date of Patent: March 30, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Maxim G. Brevnov, Hemant S. Pawar, Manohar R. Furtado, Jaiprakash G. Shewale
  • Patent number: 10954553
    Abstract: The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific PCR.
    Type: Grant
    Filed: July 12, 2016
    Date of Patent: March 23, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Shoulian Dong, Chunmei Liu
  • Patent number: 10947333
    Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.
    Type: Grant
    Filed: January 28, 2019
    Date of Patent: March 16, 2021
    Assignees: LIFE TECHNOLOGIES CORPORATION, LIFE TECHNOLOGIES AS
    Inventors: Geir Fonnum, Grete Irene Modahl, Nini Hofsloekken Kjus, Astrid Evenroed Molteberg, Diem Thuy Thi Tran, Jo Aaserud, Muhammed Gokmen, Steven M. Menchen, Carl Fuller, Luisa Andruzzi, Wolfgang Hinz
  • Patent number: 10941439
    Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.
    Type: Grant
    Filed: October 31, 2018
    Date of Patent: March 9, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Steven M. Menchen, Guobing Xiang, Prasanna Thwar, Jeffrey Frediani, Allen Wong, Alfred Lui, Lily Lu
  • Patent number: D914039
    Type: Grant
    Filed: September 18, 2020
    Date of Patent: March 23, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Sean Zimmerman, Scott Rickes, Jason Dallwig, Kathleen Free, Joseph Lee, Jennifer Hedlind
  • Patent number: D914727
    Type: Grant
    Filed: March 17, 2020
    Date of Patent: March 30, 2021
    Assignees: Life Technologies Corporation, Life Technologies Holdings PTE Limited
    Inventors: Joshua Mead, Scott Rickes, Sean Zimmerman, Alessandra Barbosa, Mio Ling, Woon Liang Soh, Kevin Mullen, Christopher Brau, Jordan Cobia, Harmon Cosme Sicat, Jr.
  • Patent number: D914906
    Type: Grant
    Filed: October 24, 2019
    Date of Patent: March 30, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Sandro Klein, Joseph Podhasky, Patrick Leung
  • Patent number: D914909
    Type: Grant
    Filed: August 16, 2018
    Date of Patent: March 30, 2021
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: David Marran, Jon A. Hoshizaki, Earl Zergiebel, Mark T. Reed