Patents Assigned to Life Technologies Corporation
  • Patent number: 10386328
    Abstract: In one embodiment, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface, a first opening extending through a first material and through a portion of a second material located on the first material and a second opening extending from the bottom of the first opening to the top of a liner layer located on the upper surface of the floating gate conductor.
    Type: Grant
    Filed: September 11, 2017
    Date of Patent: August 20, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: James Li, Jordan Owens, James Bustillo
  • Patent number: 10386290
    Abstract: The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.
    Type: Grant
    Filed: March 30, 2018
    Date of Patent: August 20, 2019
    Assignee: Life Technologies Corporation
    Inventors: Gregory Kaduchak, Michael Ward
  • Patent number: 10384208
    Abstract: A thermal cycler system for use with a sample holder configured to receive a plurality of samples includes a sample block configured to receive the sample holder, a cover lid configured to move in a direction toward the sample block from an open position to a closed position, a heated cover operatively coupled to the cover lid and configured to move in a direction toward the sample block from a raised position to a first lowered position, in which the heated cover contacts the sample holder when the sample holder is received by the sample block, and a drive assembly including a motion guide configured to move in a direction toward the sample block from a first position, wherein the cover lid is in the open position and the heated cover is in the raised position, to a second position, wherein the cover lid is in the closed position.
    Type: Grant
    Filed: December 21, 2016
    Date of Patent: August 20, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Zeqi Tan, Wuh Ken Loh, Siew Yin Lee, Kuan Moon (Bernard) Boo
  • Patent number: 10385385
    Abstract: A method for performing digital polymerase chain reaction (dPCR) is provided. The method includes partitioning a biological sample volume including a plurality of target nucleic acids into a plurality of partitions, where at least one partition includes at least one target nucleic acid. The method further includes determining a model for volume variation of the plurality of partitions and determining a number of partitions including at least one target nucleic acid. The method includes generating a concentration of target nucleic acids in the biological sample based on the model for volume variation and the fraction of partitions including at least one target nucleic acid.
    Type: Grant
    Filed: April 22, 2016
    Date of Patent: August 20, 2019
    Assignee: Life Technologies Corporation
    Inventors: Nivedita Sumi Majumdar, Swapnonil Banerjee
  • Patent number: 10385383
    Abstract: A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. The cover comprises an inner surface and is configured to be attached to the base such that the base and cover together provide a cavity containing the substrate. The inner surface includes a central area and a recessed area disposed about the central area. The central area is configured to cover the reaction regions, while the recessed area is offset from the central area in a direction normal to the inner surface.
    Type: Grant
    Filed: February 19, 2016
    Date of Patent: August 20, 2019
    Assignee: Life Technologies Corporation
    Inventors: Ronald Danehy, Gary Lim, Jacob Freudenthal
  • Patent number: 10385386
    Abstract: A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. The method further includes receiving a sample threshold cycle, determining a sample input quantity based on the first and second reference threshold cycle and the first and second target threshold cycle, and displaying the sample input quantity to a user.
    Type: Grant
    Filed: April 3, 2015
    Date of Patent: August 20, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Yalei Wu, David Keys
  • Patent number: 10385329
    Abstract: In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such embodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.
    Type: Grant
    Filed: October 17, 2017
    Date of Patent: August 20, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: John Davidson, Theo Nikiforov, Guobin Luo
  • Patent number: 10378663
    Abstract: A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. The cover fluid inlet is in fluidic communication with the base fluid outlet between the upper cavity and the lower cavity, and the cover fluid outlet provides external access from the upper cavity. A diaphragm is disposed between the housing base and the housing cover. A pinch plate is disposed in the lower cavity and comprises a pinch point disposed opposite the pinch structure. A pinch tube is in fluidic communication between the base fluid inlet and the base fluid outlet in the lower cavity.
    Type: Grant
    Filed: October 1, 2018
    Date of Patent: August 13, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventor: Jonathan Schultz
  • Patent number: 10378050
    Abstract: This disclosure relates to the use of one or more proteins (e.g., globular proteins) having a low isoelectric point and/or a limited number (e.g., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (PCR).
    Type: Grant
    Filed: January 4, 2017
    Date of Patent: August 13, 2019
    Assignee: Life Technologies Corporation
    Inventors: Jonathan Wang, David Dupont
  • Patent number: 10379079
    Abstract: A semiconductor device, comprising a first field effect transistor (FET) connected in series to a second FET, and a third FET connected in series to the first FET and the second FET. The semiconductor device further includes bias circuitry coupled to the first FET and the second FET, and an output conductor coupled to a terminal of the second FET, wherein the output conductor obtains an output signal from the second FET that is independent of the first FET.
    Type: Grant
    Filed: December 16, 2015
    Date of Patent: August 13, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventor: Keith G. Fife
  • Patent number: 10381111
    Abstract: Disclosed are devices and methods for collection, labeling and matching biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature such as a fingerprint or footprint of an individual. Such devices and methods are used in forensic, human identification, paternity, tissue typing, and screening technologies to rapidly process an individual's identity, determine the identity of an individual along with the genotype profile of the individual.
    Type: Grant
    Filed: February 14, 2017
    Date of Patent: August 13, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Michael Harrold, Lori Hennessy, Leonard Klevan
  • Publication number: 20190241942
    Abstract: Various embodiments of methods for analyzing proximity binding assay (PBA) data are disclosed. Proximity binding assays as a class of analyses offer the advantages of the sensitivity and specificity of biorecognition binding, along with the exponential signal amplification offered by a variety of oligonucleotide amplification reactions, such as the polymerase chain reaction (PCR). However, as various proximity binding assays have reaction kinetics governed by an additional step of the binding of a biorecognition probe (BRP) with a target molecule, there is a need for methods for the analysis of PBA data that are particularly suited to the unique characteristics of such data.
    Type: Application
    Filed: December 20, 2018
    Publication date: August 8, 2019
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Shiaw-Min CHEN, David W. RUFF, Harrison LEONG
  • Patent number: 10370708
    Abstract: A system and machine readable medium for nucleic acid sequencing includes disposing template polynucleotide strands in defined spaces disposed on a sensor array, at least some of the template polynucleotide strands having a sequencing primer and a polymerase operably bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed according to a predetermined ordering; and determining, for each of the series of flows of nucleotide species, how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands, wherein the predetermined ordering (a) is not a series of consecutive repetitions of a 4-flow permutation of four different nucleotide species, (b) is not specifically tailored to a particular combination of a particular template polynucleotide strand to be sequenced and a particular sequencing primer to be used, and (c) comprises a phase-protecting flow orde
    Type: Grant
    Filed: August 12, 2016
    Date of Patent: August 6, 2019
    Assignee: Life Technologies Corporation
    Inventors: Earl Hubbell, Jonathan Schultz
  • Patent number: 10371604
    Abstract: In one embodiment, a method for processing a sample includes selecting a selected sample from a biological specimen, the selected sample being in contact with a first surface of a first substrate. The method also includes transferring the selected sample directly from the first surface to a container comprising an internal volume. The method also includes forming or providing a sample solution within the internal volume of the container by contacting the selected sample with a lysis mixture using a protocol. The method further includes performing an assay, experiment, or test on the sample solution while the sample solution disposed is within the internal volume of the container. In another embodiment, a method for processing a sample includes providing a selected sample comprising one or more cells. The method also includes transferring the selected sample into an internal volume of a container.
    Type: Grant
    Filed: August 10, 2016
    Date of Patent: August 6, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Mousumi Rath, Mark Shannon, Jason La
  • Patent number: 10364465
    Abstract: The disclosure provides a plurality of nucleic acid sequences comprising multiple variants of a reference sequence. The disclosure further provides plasmids, cells, methods and kits comprising the same.
    Type: Grant
    Filed: August 15, 2014
    Date of Patent: July 30, 2019
    Assignee: Life Technologies Corporation
    Inventors: Mona Shahbazian, Kara Norman, Aron Lau, Nakul Nataraj
  • Patent number: 10365321
    Abstract: An apparatus including an array of sensors including a plurality of chemical sensors and a plurality of reference sensors, each chemical sensor coupled to a corresponding reaction region for receiving at least one reactant, and each reference sensor comprising a field effect transistor having a gate coupled to a corresponding reference line and an access circuit for accessing the chemical sensors and the reference sensors and a controller to apply bias voltages to the reference lines to select corresponding reference sensors, acquire output signals from the selected reference sensors, and identify one or more defects in the access circuit based on differences between the acquired output signals and expected output signals.
    Type: Grant
    Filed: August 19, 2015
    Date of Patent: July 30, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventor: Mark Beauchemin
  • Patent number: 10364414
    Abstract: A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. A lysis solution is passed through the filter in a second flow direction opposite the first direction, thereby dislodging the plurality of cells captured on the filter, and resuspending the plurality of cells in the lysis solution on the first side of the filter. The lysis solution lyses the plurality of cells to produce a cellular lysate. The cellular lysate is passed through the filter in the first flow direction.
    Type: Grant
    Filed: September 11, 2017
    Date of Patent: July 30, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Philip Martin, Paul Nelson, David Bourdon
  • Patent number: 10359396
    Abstract: An apparatus for preparation of an electrophoresis slab gel may include a base having an opening configured to receive a cassette configured to contain an electrophoresis slab gel, a clamping mechanism configured to move relative to the base between an open position in which the clamping mechanism permits insertion of a cassette into the base, and a closed position in which the clamping mechanism is configured to clamp a cassette received in the base, a compressible pad operatively coupled to the clamping mechanism in a position to compress against a cassette received in the base in the closed position of the clamping mechanism. The cassette may include a first plate and a second plate, and a spacer mechanism separate from each of the first and second plates, the spacer mechanism configured to be positioned between the inner faces of the first plate and the second plate.
    Type: Grant
    Filed: November 11, 2016
    Date of Patent: July 23, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Kyle Bulloch, Thomas Diller
  • Patent number: 10350554
    Abstract: A container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface, the bag having a bottom end wall being comprised of a first sheet overlying a second sheet, the first sheet and the second sheet of the bottom end wall being welded together so as to form a first sparging area and a separated second sparging area that are both bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the first and second sparging areas being gas permeable.
    Type: Grant
    Filed: March 29, 2017
    Date of Patent: July 16, 2019
    Assignee: Life Technologies Corporation
    Inventors: Michael E. Goodwin, Nephi D. Jones, Derik R. West
  • Patent number: D856528
    Type: Grant
    Filed: November 13, 2015
    Date of Patent: August 13, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Kyle Bulloch, Xin Mathers