Patents Assigned to Lonza Biologics PLC.
  • Patent number: 11326185
    Abstract: The present invention relates to stable and high-producing site-specific integration (SSI) host cells, e. g. Chinese hamster ovary (CHO)-derived host cells, methods to produce and to use them.
    Type: Grant
    Filed: October 31, 2018
    Date of Patent: May 10, 2022
    Assignees: LONZA BIOLOGICS PLC, PFIZER INC.
    Inventors: James Rance, Robert Young, Michael J. Agostino, Mark Moffat, Lin Zhang, Baohong Zhang
  • Patent number: 10669541
    Abstract: Methods for generating mammalian cells characterized by an increased concentration of at least one of miR-15, miR-16, and miR-34 for producing proteins at an industrial scale are provided. Methods for using the mammalian cells, in particular for the production of proteins of interest, are also provided.
    Type: Grant
    Filed: July 30, 2014
    Date of Patent: June 2, 2020
    Assignee: LONZA BIOLOGICS PLC.
    Inventors: Marc Feary, James Rance, Robert Young, Elizabeth C. Sayer, Christopher M. Smales
  • Patent number: 10294491
    Abstract: The present invention relates to expression vectors for the heterologous expression of a nucleic acid sequence of interest in mammalian cells, the vectors comprising a chimeric promoter regulatory sequence being operably linked to a nucleic acid sequence to be expressed, wherein the chimeric promoter regulatory sequence comprises a cytomegalovirus promoter sequence derived from murine cytomegalovirus or from human cytomegalovirus and being operably linked to the transcriptional start site of the nucleic acid sequence to be expressed; and a cytomegalovirus upstream region and/or enhancer sequence derived from human and/or the simian cytomegalovirus, wherein the upstream region and/or enhancer sequence is located 5? of and operably linked to the murine or the human promoter sequence, and wherein the chimeric promoter regulatory sequence comprises sequence elements being derived from at least two of the group consisting of murine cytomegalovirus, human cytomegalovirus and simian cytomegalovirus.
    Type: Grant
    Filed: August 28, 2017
    Date of Patent: May 21, 2019
    Assignee: LONZA BIOLOGICS PLC.
    Inventors: Tom Payne, Robert Young, Marc Feary
  • Patent number: 10280436
    Abstract: The present invention relates to stable and high-producing site-specific integration (SSI) host cells, e. g. Chinese hamster ovary (CHO)-derived host cells, methods to produce and to use them.
    Type: Grant
    Filed: June 20, 2013
    Date of Patent: May 7, 2019
    Assignees: LONZA BIOLOGICS PLC, PFIZER INC.
    Inventors: James Rance, Robert Young, Michael J. Agostino, Mark Moffat, Lin Zhang, Baohong Zhang
  • Publication number: 20190119702
    Abstract: The present invention relates to stable and high-producing site-specific integration (SSI) host cells, e. g. Chinese hamster ovary (CHO)-derived host cells, methods to produce and to use them.
    Type: Application
    Filed: October 31, 2018
    Publication date: April 25, 2019
    Applicants: LONZA BIOLOGICS PLC, PFIZER INC.
    Inventors: James RANCE, Robert YOUNG, Michael J. AGOSTINO, Mark MOFFAT, Lin ZHANG, Baohong ZHANG
  • Patent number: 10221209
    Abstract: The present invention provides a method and automated system for the purification of polypeptides including the direct filtration of solutions containing the polypeptides after purification.
    Type: Grant
    Filed: October 28, 2014
    Date of Patent: March 5, 2019
    Assignee: Lonza Biologics PLC
    Inventors: Mark R. Whickman, Sam Mansoor
  • Patent number: 10179898
    Abstract: The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.
    Type: Grant
    Filed: June 2, 2017
    Date of Patent: January 15, 2019
    Assignee: Lonza Biologics PLC
    Inventor: Mohsan Khan
  • Patent number: 10139418
    Abstract: The present invention relates to a process for the prediction of cell culture performance data of sample cells, a process for the isolation of said cells and a device for the prediction of cell culture performance data of sample cells.
    Type: Grant
    Filed: October 27, 2011
    Date of Patent: November 27, 2018
    Assignee: LONZA BIOLOGICS PLC
    Inventors: Dietmar Lang, Elaine B. Martin, Gary A. Montague, Christopher J. O'Malley, Tracy S. Root, Carol M. Trim, Jane F. Povey, Christopher M. Smales, Andrew J. Racher
  • Patent number: 9783770
    Abstract: The present invention relates to a deepwell plate system, comprising a deepwell plate and a lid system which can be detachably fitted to the deepwell plate by snap- or clamp-fastening means so as to tightly seal the deepwell plate and to a method for the cultivation of cells within the deepwell plate system according to the present invention.
    Type: Grant
    Filed: August 14, 2010
    Date of Patent: October 10, 2017
    Assignee: Lonza Biologics PLC
    Inventors: Matt Stettler, Adrian Haines
  • Patent number: 9783771
    Abstract: The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.
    Type: Grant
    Filed: October 16, 2015
    Date of Patent: October 10, 2017
    Assignee: Lonza Biologics PLC
    Inventor: Mohsan Khan
  • Patent number: 9777290
    Abstract: The present invention relates to expression vectors for the heterologous expression of a nucleic acid sequence of interest in mammalian cells, the vectors comprising a chimeric promoter regulatory sequence being operably linked to a nucleic acid sequence to be expressed, wherein the chimeric promoter regulatory sequence comprises a cytomegalovirus promoter sequence derived from murine cytomegalovirus or from human cytomegalovirus and being operably linked to the transcriptional start site of the nucleic acid sequence to be expressed; and a cytomegalovirus upstream region and/or enhancer sequence derived from human and/or the simian cytomegalovirus, wherein the upstream region and/or enhancer sequence is located 5? of and operably linked to the murine or the human promoter sequence, and wherein the chimeric promoter regulatory sequence comprises sequence elements being derived from at least two of the group consisting of murine cytomegalovirus, human cytomegalovirus and simian cytomegalovirus.
    Type: Grant
    Filed: September 23, 2013
    Date of Patent: October 3, 2017
    Assignee: Lonza Biologics Plc
    Inventors: Tom Payne, Robert Young, Marc Feary
  • Publication number: 20170242029
    Abstract: The present invention relates to a process for the prediction of cell culture performance data of sample cells, a process for the isolation of said cells and a device for the prediction of cell culture performance data of sample cells.
    Type: Application
    Filed: March 17, 2017
    Publication date: August 24, 2017
    Applicant: LONZA BIOLOGICS PLC
    Inventors: Dietmar LANG, Elaine B. MARTIN, Gary A. MONTAGUE, Christopher J. O'MALLEY, Tracy S. ROOT, Carol M. TRIM, Jane F. POVEY, Christopher M. SMALES, Andrew J. RACHER
  • Patent number: 9670446
    Abstract: The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.
    Type: Grant
    Filed: October 16, 2015
    Date of Patent: June 6, 2017
    Assignee: Lonza Biologics PLC
    Inventor: Mohsan Khan
  • Publication number: 20160177300
    Abstract: The present invention relates to the generation of mammalian cells capable of producing proteins at an industrial scale and means and methods to obtain and use said cells, in particular for protein production.
    Type: Application
    Filed: July 30, 2014
    Publication date: June 23, 2016
    Applicant: LONZA BIOLOGICS PLC
    Inventors: Marc FEARY, James RANCE, Robert YOUNG, Elizabeth C. SAYER, Christopher M. SMALES
  • Patent number: 9290563
    Abstract: This invention relates to the modification of the amino acid sequence of an immunoglobulin molecule at certain key positions within regions of the VH and VL FR and CDR3 domains and/or the CH1 domain which are prone to aggregation. Immunoglobulins modified as described may display improved manufacturability, for example, reduced aggregation propensity and/or increased production levels.
    Type: Grant
    Filed: January 5, 2015
    Date of Patent: March 22, 2016
    Assignee: Lonza Biologics PLC
    Inventors: Andreas Arnell, Jose Jimenez, Rebecca Michael, Yvette Stallwood, Jesus Zurdo
  • Publication number: 20150167020
    Abstract: The present invention relates to stable and high-producing site-specific integration (SSI) host cells, e.g. Chinese hamster ovary (CHO)-derived host cells, methods to produce and to use them.
    Type: Application
    Filed: June 20, 2013
    Publication date: June 18, 2015
    Applicants: LONZA BIOLOGICS PLC, PFIZER INC.
    Inventors: James Rance, Robert Young, Michael J. Agostino, Mark Moffat, Lin Zhang, Baohong Zhang
  • Publication number: 20150105542
    Abstract: The present invention provides a method and automated system for the purification of polypeptides including the direct filtration of solutions containing the polypeptides after purification.
    Type: Application
    Filed: October 28, 2014
    Publication date: April 16, 2015
    Applicant: Lonza Biologics PLC
    Inventors: Mark R. Whickman, Sam Mansoor
  • Patent number: 8951516
    Abstract: This invention relates to the modification of the amino acid sequence of an immunoglobulin molecule at certain key positions within regions of the VH and VL FR and CDR3 domains and/or the CH1 domain which are prone to aggregation. Immunoglobulins modified as described may display improved manufacturability, for example, reduced aggregation propensity and/or increased production levels.
    Type: Grant
    Filed: August 20, 2010
    Date of Patent: February 10, 2015
    Assignee: Lonza Biologics PLC
    Inventors: Andreas Arnell, Jose Jimenez, Rebecca Michael, Yvette Stallwood, Jesus Zurdo
  • Patent number: 8906231
    Abstract: The present invention provides a method and automated system for the purification of polypeptides including the direct filtration of solutions containing the polypeptides after purification.
    Type: Grant
    Filed: September 10, 2010
    Date of Patent: December 9, 2014
    Assignee: Lonza Biologics PLC
    Inventors: Mark R. Whickman, Sam Mansoor
  • Publication number: 20130295596
    Abstract: The present invention relates to a process for the prediction of cell culture performance data of sample cells, a process for the isolation of said cells and a device for the prediction of cell culture performance data of sample cells.
    Type: Application
    Filed: October 27, 2011
    Publication date: November 7, 2013
    Applicant: LONZA BIOLOGICS PLC
    Inventors: Dietmar Lang, Elaine B. Martin, Gary A. Montague, Christopher J. O'Malley, Tracy S. Root, Carol M. Trim, Jane F. Povey, Christopher M. Smales, Andrew J. Racher