Publication number: 20240287487
Abstract: Aspects of this disclosure provide compositions, strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. Fusion proteins capable of inducing a cytosine (C) to guanine (G) change (i.e., transversion changes) in a nucleic acid (e.g., genomic DNA) are provided. Fusion proteins of a nucleic acid programmable DNA binding protein (e.g., Cas9) and nucleic acid editing proteins or protein domains, e.g., deaminase domains, polymerase domains, base excision enzymes, and/or DNA repair proteins, are also provided. Methods for targeted nucleic acid editing are also provided. Reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of a nucleic acid programmable DNA binding protein (e.g., Cas9), and nucleic acid editing proteins or domains, are further provided in the present disclosure.
Type:
Application
Filed:
June 10, 2022
Publication date:
August 29, 2024
Applicants:
The Broad Institute, Inc., President and Fellows of Harvard College, The Regents of the University of California, Massachusetts Institute of Technology
Inventors:
Luke W. Koblan, David R. Liu, Mandana Arbab, Max Walt Shen, Andrew Vito Anzalone, Jeffrey Hussmann