Abstract: A composite liquid filtration platform including a composite filtration medium featuring an electrospun polymeric nanofiber layer collected on a porous membrane. When in use, the porous membrane acts as a prefilter used upstream from the polymeric nanofiber layer to remove particles from a liquid stream flowing through the composite filtration structure. The nanofiber layer, positioned downstream from the porous membrane, is used as the retentive layer for critical filtration to provide biosafety assurance, and is responsible for capturing microorganisms like bacteria, mycoplasma or viruses. The composite liquid filtration platform provided herein exhibits permeability advantages over conventional porous membranes or nanofiber mats spun on coarse non-wovens.

Abstract: A needle protector, having a flexible material, a pocket defining an internal volume, a wrapping strap having a plurality of holes, and a main body disposed between the pocket and the wrapping strap; at least one rigid body or cylinder for placement within the internal volume; and; a pin for placement within one of the plurality of holes, wherein the pin is capable of releasabiy locking the pocket and the main body when the wrapping strap is wrapped around the pocket and the main body.

Abstract: The invention concerns an installation for treating biological liquid by chromatography, extending in a longitudinal direction and comprising a supply valve (20b), a supply pump (30) downstream of the valve, instrument members downstream of the pump among which are at least one distribution valve (81a-c, 82a-c, 83a-c) and at least one device (78a-c, 85a-c, 86a-c) for measuring a physico-chemical parameter of the liquid, at least one chromatography column (99a-c) downstream of the instrument members and of the pipes connecting the valve, the pump, the instrument members and the column, instrument members being associated with the chromatography column and each mounted on at least one dedicated control and actuation platform (80a-c), which extends in a vertical direction relative to the generally longitudinal direction of extension of said installation, and which are disposed substantially above each other on said dedicated control and actuation platform.

Abstract: A multilayered film for biocontainers, an abrasion-resistant substrate attached to the interior or the exterior side of the film wherein the substrate is one of a polymeric woven fibrous material or a polymeric non-woven fibrous material, a woven fibrous material or a non-woven fibrous material having metal fibers, a woven fibrous material or a non-woven fibrous material having glass fibers, or a woven fibrous material or a non-woven fibrous material having carbon fibers; a polymer backing to attach the substrate to the film, including one of polyolefins, polyurethanes and nylons; an outer protective layer disposed on the outside of the substrate to encapsulate the substrate; and a gas impermeable layer that includes ethylene vinyl alcohol disposed intermediate to the substrate and the outer protective layer.

Abstract: A container and a container engaging member. The container engaging member may include a sample holder or reservoir, a filtration element and collar. In the assembled condition, the sample holder or reservoir is upstream of the filtration element, the container is downstream of the filtration element, and the sample holder or reservoir is attached to the container. The container engagement member is engageable and disengageable from the bottle or container in a quick attach, quick release manner, such as with only a 90 degree, ¼ turn. A tactile and/or audible indication that the engagement is complete is provided.

Abstract: A clamp device for a barb member having a barbed region for disposing within an end of a flexible tube having an outer surface. The clamp device includes a flexible member including a body having a plurality of finger members compressible between a first resting position and a second clamping position; at least one of the flexible finger members having a radially inwardly extending tooth; the body having a through bore and an outer surface comprising threads; the flexible member being slidable over the barbed region and the outer surface of the flexible tube; a compression member having a body having a through bore and an inner surface including threads; the compression member being slidable over the flexible member; wherein upon threadingly engaging the compression member with the flexible member, the compression member compresses the finger member against the barb member.

Abstract: The present invention relates to chromatography ligands having improved caustic stability, e.g., ligands based on immunoglobulin-binding proteins such as, Staphylococcal protein A, as well as methods of making and using such ligands.

Abstract: Instrumenting or modeling a bio-manufacturing process is disclosed. A data store holds information regarding one or more bio-manufacturing processes. For each process, the data store information includes workflow steps and per step indications of required equipment, services and consumables according to best practice and/or experts. A videographic user interface enables user interactive input regarding a subject bio-manufacturing process from the data store. The user-interface presents a model representation of deployment of the subject bio-manufacturing process. The user interactively navigates and makes selections in the displayed model representation. The user selections include certain bio-manufacturing products, equipment and services from an image database. The images from the image database enable the views of the model representation to appear true to life demonstrating equipment/product layout and physical equipment/computer connections used in the subject bio-manufacturing process.

Abstract: The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass tangential flow filtration assemblies, scalable single-pass tangential flow filtration assemblies and adaptable modular single-pass tangential flow filtration assemblies. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass tangential flow filtration assembly and for cleaning a tangential flow filtration assembly. In additional embodiments, the invention provides methods of increasing the processing capacity of a single-pass tangential flow filtration assembly.

Abstract: Microfluidic devices, systems, and methods providing for an invasion assay using microfluidic culture systems.

Abstract: A method of filtering a liquid feed is described, comprising passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers. A method of filtering a liquid feed is also described comprising passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods of the invention can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.

Abstract: Treatment methods for reduction of (1?3)-?-D-glucan leachables from cellulose-containing filter materials are described.

Abstract: The present invention provides methods for cleaning a Protein A chromatography column employing a media comprising a Protein A ligand derived from the C domain of Staphylococcus aureus, such that the column can be cleaned using both acidic and alkaline solutions.

Abstract: A method of integrity testing porous materials that is non-destructive to the material being tested. The inlet gas stream includes at least two gases, wherein one of the gases has a different permeability in liquid than the other, such as oxygen and nitrogen in water. The relative permeability of the gases is measured in the retentate stream, thereby avoiding accessing the permeate stream and potentially introducing contaminants to the material being tested. The integrity test is capable of detecting the presence of oversized pores or defects that can compromise the retention capability of the porous material.

Abstract: Fluid transfer device and method that allows for sterile wet connections, allows the connection to be reversed, and allows the connection to be reconnected, while leaving the connectors sterile and reusable under pressure. In certain embodiments, the device includes a first member and a second member, the latter adapted to receive the former in locking engagement upon actuation of the device to create fluid communication between the two in a sterile manner. Each of the members include a door that when opened, allows the first member to be linearly displaced into the second member to allow fluid to be transferred. When fluid transfer is complete, the first member can be retracted from the second member, and the doors closed.

Abstract: The method, device, and system relate to particle analysis, and in particular, to a microfluidic device designed for trapping particles for analysis. Particles include beads and cells.

Abstract: The present invention relates to improved processes and systems for purification of biological molecules, where the processes can be performed in a continuous manner.