Patents Assigned to Molecular Devices Corporation
  • Patent number: 7745142
    Abstract: Assays for detecting molecular modifications such as phosphate modifications and the presence and/or activity of enzymes and other agents involved in facilitating or otherwise regulating such modifications.
    Type: Grant
    Filed: December 23, 2003
    Date of Patent: June 29, 2010
    Assignee: Molecular Devices Corporation
    Inventors: Annegret Boge, Luke D. Lavis, J. Richard Sportsman, Merl F. Hoekstra, Wei Huang
  • Publication number: 20090081652
    Abstract: The present invention relates to agonist-independent methods of screening for compounds that alter GPCR desensitization. Included in the present invention are cell lines containing GRKs, in which GPCRs are desensitized in the absence of agonist; the GRKs may be modified. The present invention relates to methods to determine if a GPCR is expressed at the plasma membrane, and if the GPCR has an affinity for arrestin. Modified GPCRs which have increased arrestin affinity are included in the present invention. These modified GPCRs are useful in methods to screen for compounds that alter desensitization, including both the agonist-independent methods and agonist-dependent methods described herein.
    Type: Application
    Filed: November 8, 2007
    Publication date: March 26, 2009
    Applicant: Molecular Devices Corporation
    Inventors: Robert H. Oakley, Christine C. Hudson
  • Publication number: 20090082410
    Abstract: The present invention relates to agonist-independent methods of screening for compounds that alter GPCR desensitization. Included in the present invention are cell lines containing GRKs, in which GPCRs are desensitized in the absence of agonist; the GRKs may be modified. The present invention relates to methods to determine if a GPCR is expressed at the plasma membrane, and if the GPCR has an affinity for arrestin. Modified GPCRs which have increased arrestin affinity are included in the present invention. These modified GPCRs are useful in methods to screen for compounds that alter desensitization, including both the agonist-independent methods and agonist-dependent methods described herein.
    Type: Application
    Filed: November 8, 2007
    Publication date: March 26, 2009
    Applicant: Molecular Devices Corporation
    Inventors: Robert H. Oakley, Christine C. Hudson
  • Patent number: 7455982
    Abstract: Methods of detecting G protein-coupled receptor (GPCR) activity in vitro and in vivo are provided. In one embodiment, the method includes providing at least one cell that expresses a GPCR and a plurality of conjugated proteins. Each of the plurality of conjugated proteins is formed by conjugating an arrestin protein and a detectable molecule. The plurality of conjugated proteins are substantially evenly distributed in the cytoplasm of the at least one cell. A first image of the at least one cell is obtained by detecting an amount of energy emitted from the detectable molecules and storing a value relative to the amount of energy. The at least one cell is treated with an agonist. A second image of the at least one cell is obtained. The first image and the second image are compared to detect the localization of at least some of the plurality of conjugated proteins at endocytic vesicles and/or endosomes.
    Type: Grant
    Filed: March 21, 2005
    Date of Patent: November 25, 2008
    Assignee: Molecular Devices Corporation
    Inventors: Larry S. Barak, Robert H. Oakley
  • Publication number: 20080285036
    Abstract: Disclosed are photometric methods and devices for determining optical pathlength of liquid samples containing analytes dissolved or suspended in a solvent. The methods and devices rely on determining a relationship between the light absorption properties of the solvent and the optical pathlength of liquid samples containing the solvent. This relationship is used to establish the optical pathlength for samples containing an unknown concentration of analyte but having similar solvent composition. Further disclosed are methods and devices for determining the concentration of analyte in such samples where both the optical pathlength and the concentration of analyte are unknown. The methods and devices rely on separately determining, at different wavelengths of light, light absorption by the solvent and light absorption by the analyte. Light absorption by the analyte, together with the optical pathlength so determined, is used to calculate the concentration of the analyte.
    Type: Application
    Filed: February 25, 2008
    Publication date: November 20, 2008
    Applicant: Molecular Devices Corporation
    Inventors: Dean G. Hafeman, Calvin Y. Chow
  • Patent number: 7387715
    Abstract: Systems for positioning and/or analyzing samples such as cells, vesicles, cellular organelles, and fragments, derivatives, and mixtures thereof, for electrical and/or optical analysis, especially relating to the presence and/or activity of ion channels.
    Type: Grant
    Filed: December 31, 2002
    Date of Patent: June 17, 2008
    Assignee: Molecular Devices Corporation
    Inventors: Horst Vogel, Christian Schmidt
  • Patent number: 7364846
    Abstract: Methods and compositions relating to the generation and use of gene expression data from tissue samples that have been fixed and embedded are provided. The data can electronically stored and implemented as well as used to augment diagnosis and treatment of diseases.
    Type: Grant
    Filed: December 23, 2002
    Date of Patent: April 29, 2008
    Assignee: Molecular Devices Corporation
    Inventors: Mark G. Erlander, Ranelle C. Salunga
  • Patent number: 7358064
    Abstract: The invention encompasses kits for an improved method for measuring membrane potential using compounds of the formula I as potentiometric probes. These probes may be used in combination with other fluorescent indicators such as Indo-1, Fura-2, and Fluo-3, such probes may be used in microplate reading devices such as FLIPRâ„¢, fluorescent imaging plate reader, sold by Molecular Devices Corp., of Sunnyvale, Calif.; flow cytometers; and fluorometers. Such probes are used to measure membrane potential in live cells wherein X is O or S; and n is 1 or 2.
    Type: Grant
    Filed: December 22, 2004
    Date of Patent: April 15, 2008
    Assignee: Molecular Devices Corporation
    Inventors: Dieter Klaubert, Zhenjun Diwu, Guoliang Yi, Martin Kirk
  • Patent number: 7332292
    Abstract: The present invention relates to agonist-independent methods of screening for compounds that alter GPCR desensitization. Included in the present invention are cell lines containing GRKs, in which GPCRs are desensitized in the absence of agonist; the GRKs may be modified. The present invention relates to methods to determine if a GPCR is expressed at the plasma membrane, and if the GPCR has an affinity for arrestin. Modified GPCRs which have increased arrestin affinity are included in the present invention. These modified GPCRs are useful in methods to screen for compounds that alter desensitization, including both the agonist-independent methods and agonist-dependent methods described herein.
    Type: Grant
    Filed: February 26, 2004
    Date of Patent: February 19, 2008
    Assignee: Molecular Devices Corporation
    Inventors: Robert H. Oakley, Christine C. Hudson
  • Patent number: 7297503
    Abstract: Described herein are methods of identifying a transmembrane receptor (TMR) agonist and compounds identified by this method. The TMR agonist (TMRA) is capable of activating TMR signaling while exhibiting reduced TMR internalization over a control compound.
    Type: Grant
    Filed: October 24, 2003
    Date of Patent: November 20, 2007
    Assignee: Molecular Devices Corporation
    Inventors: Carson R Loomis, Robert H. Oakley, Shuntai Wang, Allen E. Eckhardt
  • Publication number: 20070209935
    Abstract: Systems for positioning and/or analyzing samples such as cells, vesicles, cellular organelles, and fragments, derivatives, and mixtures thereof, for electrical and/or optical analysis, especially relating to the presence and/or activity of ion channels.
    Type: Application
    Filed: September 25, 2006
    Publication date: September 13, 2007
    Applicant: Molecular Devices Corporation
    Inventors: Horst VOGEL, Christian Schmidt
  • Patent number: 7265829
    Abstract: A reflective light imaging system for use in high-throughput screening of samples disposed in multiple-well plates. The system can include a set of mirrors and lenses. The first mirror has a central aperture through which light from the object passes. The first mirror has a concave reflective surface that faces the image plane. The next element is a second mirror with a convex reflective surface. The system can include an aberration corrective system positioned between the second mirror and the image plane, and an optical sensor near the image plane. Light from an object passes through the central aperture of the first mirror and is reflected off the convex surface of the second mirror. The light then strikes the reflective surface of the first mirror. The light from the first mirror is then collected by the aberration correction system and transmitted toward the image plane.
    Type: Grant
    Filed: December 17, 2003
    Date of Patent: September 4, 2007
    Assignee: Molecular Devices Corporation
    Inventors: Wu Jiang, Todd E. French, David P. Stumbo
  • Patent number: 7244349
    Abstract: Systems for positioning and/or analyzing samples such as cells, vesicles, cellular organelles, and fragments, derivatives, and mixtures thereof, for electrical and/or optical analysis, especially relating to the presence and/or activity of ion channels.
    Type: Grant
    Filed: August 27, 2002
    Date of Patent: July 17, 2007
    Assignee: Molecular Devices Corporation
    Inventors: Horst Vogel, Christian Schmidt
  • Patent number: 7229595
    Abstract: This relates to filter columns for isolating nucleic acids. The filter column is adapted for stable placement within the upper portion of standard plastic collection tubes of various sizes. The body of the filter column has a number of surfaces to accommodate placement within variously sized collection tubes. The filter column contains nucleic acid-specific filter which can be located at alternate regions within the filter column, providing different filter surface areas and loading volume capacities using the same column body. The filter column has an opening on an upper end adapted to be sealed by a cap. A method for recovering nucleic acids using such filter column is also provided.
    Type: Grant
    Filed: June 15, 2001
    Date of Patent: June 12, 2007
    Assignee: Molecular Devices Corporation
    Inventors: Derrick A. Richardson, Derek S. Pai, Jorge Monteon
  • Patent number: 7201836
    Abstract: Systems for positioning and/or analyzing samples such as cells, vesicles, cellular organelles, and fragments, derivatives, and mixtures thereof, for electrical and/or optical analysis, especially relating to the presence and/or activity of ion channels.
    Type: Grant
    Filed: March 7, 2002
    Date of Patent: April 10, 2007
    Assignee: Molecular Devices Corporation
    Inventors: Horst Vogel, Christian Schmidt
  • Patent number: 7163800
    Abstract: The methods of the present invention allow the screening of a test composition for non-receptor-specific GPCR desensitization inhibitory activity. The methods involve screening a test composition for an indication of GPCR desensitization inhibitory activity against two or more GPCRs that are different from each other. When there is an indication that a particular test composition has GPCR desensitization inhibitory activity with respect to each of the two or more GPCRs that are different from one another, then, according to the present invention, there is an indication that the test composition has non-receptor-specific GPCR desensitization inhibitory activity.
    Type: Grant
    Filed: August 1, 2003
    Date of Patent: January 16, 2007
    Assignee: Molecular Devices Corporation
    Inventors: Robert H. Oakley, Lawrence S. Barak, Stephane A. Laporte, Marc G. Caron
  • Publication number: 20060238764
    Abstract: Disclosed are photometric methods and devices for determining optical pathlength of liquid samples containing analytes dissolved or suspended in a solvent. The methods and devices rely on determining a relationship between the light absorption properties of the solvent and the optical pathlength of liquid samples containing the solvent. This relationship is used to establish the optical pathlength for samples containing an unknown concentration of analyte but having similar solvent composition. Further disclosed are methods and devices for determining the concentration of analyte in such samples where both the optical pathlength and the concentration of analyte are unknown. The methods and devices rely on separately determining, at different wavelengths of light, light absorption by the solvent and light absorption by the analyte. Light absorption by the analyte, together with the optical pathlength so determined, is used to calculate the concentration of the analyte.
    Type: Application
    Filed: December 13, 2005
    Publication date: October 26, 2006
    Applicant: Molecular Devices Corporation
    Inventors: Dean Hafeman, Calvin Chow
  • Patent number: 7070921
    Abstract: Assays for detecting molecular modifications such as phosphate modifications and the presence and/or activity of enzymes and other agents involved in facilitating or otherwise regulating such modifications.
    Type: Grant
    Filed: April 27, 2001
    Date of Patent: July 4, 2006
    Assignee: Molecular Devices Corporation
    Inventors: Wei Huang, Merl F. Hoekstra, J. Richard Sportsman, Ewald A. Terpetschnig
  • Patent number: 6995844
    Abstract: Disclosed are photometric methods and devices for determining optical pathlength of liquid samples containing analytes dissolved or suspended in a solvent. The methods and devices rely on determining a relationship between the light absorption properties of the solvent and the optical pathlength of liquid samples containing the solvent. This relationship is used to establish the optical pathlength for samples containing an unknown concentration of analyte but having similar solvent composition. Further disclosed are methods and devices for determining the concentration of analyte in such samples where both the optical pathlength and the concentration of analyte are unknown. The methods and devices rely on separately determining, at different wavelengths of light, light absorption by the solvent and light absorption by the analyte. Light absorption by the analyte, together with the optical pathlength so determined, is used to calculate the concentration of the analyte.
    Type: Grant
    Filed: December 10, 2002
    Date of Patent: February 7, 2006
    Assignee: Molecular Devices Corporation
    Inventors: Dean G. Hafeman, Calvin Y. Chow
  • Patent number: 6992761
    Abstract: Broad-range light-detection systems, including components and methods of use thereof. These systems may include apparatus and methods for detecting light with increased speed and/or detection efficiency, particularly in applications involving repeated analysis of the same sample and/or successive analysis of different samples, and particularly when the sample or samples have a wide range of light intensities. These systems also may include apparatus and methods for detecting light with increased accuracy over a broad range of intensities. These systems also may include vapparatus and methods for automatically scaling detection range to improve detection based on the intensity of the detected light.
    Type: Grant
    Filed: May 22, 2003
    Date of Patent: January 31, 2006
    Assignee: Molecular Devices Corporation
    Inventors: Douglas N. Modlin, David P. Stumbo, Rick V. Stellmacher, Jonathan F. Petersen, Todd E. French