Patents Assigned to Mycogen Plant Science, Inc.
-
Patent number: 7214861Abstract: A plant promoter is provided comprising the regulatory region of a legume Enod2 gene, a gene expressed in nodule tissue of legumes in the early stages of nodulation. The promoter operates to express foreign genes under its control in developing nodules. The sequence of two soybean Enod2 genes with their regulatory regions is provided.Type: GrantFiled: January 2, 2004Date of Patent: May 8, 2007Assignee: Mycogen Plant Science, Inc.Inventors: Henk J. Franssen, Anton H. Blsseling
-
Patent number: 6943282Abstract: A method for expressing insecticidal protein structural genes in plant genomes is provided. In the preferred embodiments this invention comprises placing a structural gene for the Bacillus thuringiensis crystal protein under control of a plant or a T-DNA promoter and ahead of a polyadenylation site followed by insertion of said promoter/structural gene combination into a plant genome by utilizing an Agrobacterium tumefaciens Ti plasmid-based transformation system. The modified Ti plasmid is then used to transform recipient plant cells. Also provided are the plants and tissues produced by this method and bacterial strains, plasmids, and vectors useful for execution of this invention.Type: GrantFiled: June 10, 1991Date of Patent: September 13, 2005Assignee: Mycogen Plant Science, Inc.Inventors: Michael J. Adang, John D. Kemp
-
Patent number: 6914176Abstract: Disclosed herein is a subgroup of corn lines comprised of plants that produce seeds having low saturated fatty acid content. The plants disclosed herein can be used to produce low saturated corn material predictably, via conventional methods. Further, the plants disclosed herein can be used to produce commercially acceptable hybrids having lower saturated fat content.Type: GrantFiled: June 16, 1999Date of Patent: July 5, 2005Assignee: Mycogen Plant Science, IncInventor: Bruce Nagel
-
Patent number: 6444879Abstract: The present invention provides a novel Brassica napus oleifera annua variety, an agronomically superior high oleic canola variety having a unique fatty acid profile, designated “1709”. Also provided in the present invention are seeds of canola variety 1709, plants produced from seeds of 1709, and tissue cultures of regenerable cells of the canola plant grown from 1709 seed.Type: GrantFiled: November 18, 1999Date of Patent: September 3, 2002Assignee: Mycogen Plant Science, Inc.Inventor: John Lawrence Sernyk
-
Patent number: 6376234Abstract: The present invention relates to a novel method of inserting viral DNA, which optionally may contain cargo-DNA, into plants or viable parts thereof, but preferably into plants of the monocotyledon class, and most preferably into plants of the family Gramineae, using suitable transfer microorganisms. Further comprised by the invention are recombinant DNA, plasmid and vector molecules suitably adapted to the specific conditions of the process according to the invention and the transgenic plant products obtainable in accordance with the said process.Type: GrantFiled: October 28, 1996Date of Patent: April 23, 2002Assignees: Ciba-Geigy, Mycogen Plant Science, Inc.Inventors: Nigel Harry Grimsley, Barbara Hohn, Thomas Hohn, Jeffrey William Davies, Margaret Irene Boulton
-
Patent number: 6251656Abstract: The Bacillus thuringiensis var. kurstaki HD-73 crystal protein gene was cloned into pBR322. E. coli cells harboring this recombinant plasmid produced a 130 kD protoxin that was toxic to Manduca sexta (tobacco hornworm) larvae. Plasmids having the 3′-end of the protoxin gene deleted where also constructed. E. coli cells harboring these deleted plasmids produced an active, soluble 68 kD toxin, provided that the 3′-deletion had not removed sequences encoding the 68 kD toxin. The invention provides methods to produce 68 kD toxin protein by constructing partial protoxin genes encoding the toxin followed by expression of the genes in living cells. Useful plasmids and cells are also provided.Type: GrantFiled: October 21, 1999Date of Patent: June 26, 2001Assignee: Mycogen Plant Science, Inc.Inventor: Michael J. Adang
-
Patent number: 6229004Abstract: The Bacillus thuringiensis var. kurstaki HD-73 crystal protein gene was cloned into pBR322. E. coli cells harboring this recombinant plasmid produced a 130 kD protoxin that was toxic to Manduca sexta (tobacco hornworm) larvae. Plasmids having the 3′-end of the protoxin gene deleted where also constructed. E. coli cells harboring these deleted plasmids produced an active, soluble 68 kD toxin, provided that the 3′-deletion had not removed sequences encoding the 68 kD toxin. The invention provides methods to produce 68 kD toxin protein by constructing partial protoxin genes encoding the toxin followed by expression of the genes in living cells. Useful plasmids and cells are also provided.Type: GrantFiled: July 10, 2000Date of Patent: May 8, 2001Assignee: Mycogen Plant Science, Inc.Inventor: Michael J. Adang
-
Patent number: 6114138Abstract: The Bacillus thuringiensis var. kurstaki HD-73 crystal protein gene was cloned into pBR322. E. coli cells harboring this recombinant plasmid produced a 130 kD protoxin that was toxic to Manduca sexta (tobacco hornworm) larvae. Plasmids having the 3'-end of the protoxin gene deleted where also constructed. E. coli cells harboring these deleted plasmids produced an active, soluble 68 kD toxin, provided that the 3'-deletion had not removed sequences encoding the 68 kD toxin. The invention provides methods to produce 68 kD toxin protein by constructing partial protoxin genes encoding the toxin followed by expression of the genes in living cells. Useful plasmids and cells are also provided.Type: GrantFiled: June 4, 1984Date of Patent: September 5, 2000Assignee: Mycogen Plant Science, Inc.Inventor: Michael J. Adang
-
Patent number: 6111070Abstract: The sequence of the T-DNA of the octopine-type Ti plasmid found in Agrobacterium tumefaciens ATCC 15955 is disclosed. Fourteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found. The use of promoters and polyadenylation sites from pTi15955 to control expression of foreign structural genes is taught, using as examples the structural genes for the Phaseolus vulgaris storage protein phaseolin, P. vulgaris lectin, thaumatin, and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.Type: GrantFiled: June 2, 1995Date of Patent: August 29, 2000Assignee: Mycogen Plant Science, Inc.Inventors: John D. Kemp, Richard F. Barker
-
Patent number: 6090627Abstract: The sequence of the T-DNA of the octopine-type Ti plasmid found in Agrobacterium tumefaciens ATCC 15955 is disclosed. Fourteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found. The use of promoters and polyadenylation sites from pTi15955 to control expression of foreign structural genes is taught, using as examples the structural genes for the Phaseolus vulgaris storage protein phaseolin, P. vulgaris lectin, thaumatin, and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.Type: GrantFiled: June 2, 1995Date of Patent: July 18, 2000Assignee: Mycogen Plant Science, Inc.Inventors: John D. Kemp, Richard F. Barker, Michael J. Adang
-
Patent number: 6054574Abstract: A DNA segment from the upstream untranscribed region of a maize ubiquitin gene is disclosed. This ubiquitin promoter region, which comprises heat shock consensus elements, initiates and regulates the transcription of genes placed under its control. Recombinant DNA molecules are also described in which a ubiquitin promoter is combined with a plant expressible structural gene for regulated expression of the structral gene and for regulated control of expression when stressed with clevated temperatures. Such recombinant DNA molecules are introduced into plant tissue so that the promoter/stuctural gene combination is expressed.Type: GrantFiled: June 9, 1998Date of Patent: April 25, 2000Assignee: Mycogen Plant Science, Inc.Inventors: Peter H. Quail, Alan H. Christensen, Howard P. Hershey, Robert A. Sharrock, Thomas D. Sullivan
-
Patent number: 6037526Abstract: The present invention relates to a novel method of inserting viral DNA, which optionally may contain cargo-DNA, into plants or viable parts thereof, but preferably into plants of the monocotyledon class, and most preferably into plants of the family Gramineae, using suitable transfer microorganisms. Further comprised by the invention are recombinant DNA, plasmid and vector molecules suitably adapted to the specific conditions of the process according to the invention and the transgenic plant products obtainable in accordance with the said process.Type: GrantFiled: October 28, 1996Date of Patent: March 14, 2000Assignees: Ciba-Geigy, Mycogen Plant Science, Inc.Inventors: Nigel Harry Grimsley, Barbara Hohn, Thomas Hohn, Jeffrey William Davies, Margaret Irene Boulton
-
Patent number: 6020190Abstract: A method of inducibly enhancing the constitutive expression of a DNA sequence of interest is described in which plant cells are transformed with a DNA sequence of interest that is operably joined to a plant ubiquitin regulatory region comprised of a heat shock element, a promoter, a transcription start site, an intron and a translation start site, When monocot or dicot plant cells are subjected to permissive heat shock temperatures, the level of expression of the DNA sequence of interest is enhanced.Type: GrantFiled: November 18, 1996Date of Patent: February 1, 2000Assignee: Mycogen Plant Science, Inc.Inventors: Peter H. Quail, Alan H. Christensen, Howard P. Hershey, Robert A. Sharrock, Thomas D. Sullivan
-
Patent number: 6015891Abstract: Synthetic Baccilus thuringiensis toxin genes designed to be expressed in plants at a level higher than naturally-occurring Bt genes are provided. These genes utilize codons preferred in highly expressed monocot or dicot proteins.Type: GrantFiled: August 29, 1996Date of Patent: January 18, 2000Assignee: Mycogen Plant Science, Inc.Inventors: Michael J. Adang, Elizabeth E. Murray
-
Patent number: 6013523Abstract: Synthetic Baccilus thuringiensis toxin genes designed to be expressed in plants at a level higher than naturally-occurring Bt genes are provided. These genes utilize codons preferred in highly expressed monocot or dicot proteins.Type: GrantFiled: August 29, 1996Date of Patent: January 11, 2000Assignee: Mycogen Plant Science, Inc.Inventors: Michael J. Adang, Elizabeth E. Murray
-
Patent number: 5846795Abstract: A +strand RNA viral transformation of host organisms with foreign RNA, and expression of said foreign RNA. The foreign RNA is inserted into an infective RNA viral segment containing replication elements, and allowed to infect the host organism. The invention is exemplified utiliing brome mosaic RNA modified to contain a gene coding for chloramphenicol acetyl transferase (CAT) in the transformation of barley protoplasts.Type: GrantFiled: June 5, 1995Date of Patent: December 8, 1998Assignee: Mycogen Plant Science, Inc.Inventors: Paul G. Ahlquist, Roy C. French
-
Patent number: 5824866Abstract: The sequence of the T.sub.L -DNA of Ri plasmids found in Agrobacterium rhizogenes strains HRI and A4 is disclosed. Sixteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found, five of which were observed to be transcripted in a developmentally and phenotypically regulated manner. The use of promoters and polyadenylation sites from pRi T.sub.L -DNA to control expression of heterologous foreign structural genes is taught, using as examples the structural genes for Phaseolus vulgaris storage protein (phaseolin), P. vulgaris lectin, a sweet protein (thaumatin), and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.Type: GrantFiled: June 2, 1995Date of Patent: October 20, 1998Assignee: Mycogen Plant Science, Inc.Inventors: Jerry L. Slightom, David A. Tepfer
-
Patent number: 5807998Abstract: A protein and gene encoding it are disclosed which confer sensitivity to B. maydis T toxin and the insecticide methomyl, in cells carrying the gene and expressing the protein. Toxin sensitivity domains of the protein have been identified wherein a modification yields a toxin-insensitive product.Type: GrantFiled: April 21, 1995Date of Patent: September 15, 1998Assignees: Mycogen Plant Science, Inc., North Carolina State UniversityInventors: Charles S. Levings, III, Ralph E. Dewey, Carl J. Braun
-
Patent number: 5804439Abstract: A recombinant RNA virus is provided allowing encapsidation of genetically engineered viral sequences in heterologous, preferably rod-shaped coat, protein capsids. Since icosahedral viruses are limited in the amount of RNA they can carry, and rod-shaped viruses are expansible, this invention allows the size of recombinant virus RNA components to be increased (or decreased). Methods of making and using such recombinant viruses are also provided, specifically with respect to the transfection of plants to bring about genotypic and phenotypic changes.Type: GrantFiled: December 27, 1996Date of Patent: September 8, 1998Assignee: Mycogen Plant Science, Inc.Inventors: Paul G. Ahlquist, Roy C. French, Robert F. Sacher
-
Patent number: 5767339Abstract: According to the invention, there is provided an inbred corn line designated 85857. Further provided are the plants and seeds of inbred corn line 85857, and hybrids produced using 85857 inbred line as one parent crossed with a distinct inbred corn line.Type: GrantFiled: June 7, 1995Date of Patent: June 16, 1998Assignee: Mycogen Plant Science, Inc.Inventor: Leroy McCurdy