Abstract: The present inventors aimed to identify the gene responsible for the temperature-sensitive male sterility trait in “PL12” and to provide a method for producing conditional male sterile plants targeting the gene, and performed whole genome sequence analysis on “PL12” and the original variety thereof to compare the two. As a result, it was found that there was a deletion of about 150 kb in “PL12” chromosome 7. Then, as for this region, six types of partial deletion lines were produced by genome editing, and the presence or absence of the temperature-sensitive male sterility trait in each line was used as an index to successfully narrow the range, containing the responsible mutation, down to about 10 kb. Furthermore, as a result of creating a line with genome editing-disrupted functions of the two genes located in this region, the gene responsible for the temperature-sensitive male sterility trait was successfully identified.

Abstract: Developed and provided is a method of collecting a large amount of high-quality bagworm silk threads having no contaminant from bagworm nests in a convenient manner and at low cost. The habit of bagworms is utilized to allow a bagworm to build a nest using solvent-soluble substances or thermally meltable substances as nest materials, followed by dissolving or melting the nest materials to separate the nest material from the bagworm silk threads, whereby only pure bagworm silk threads constituting the bagworm nest can be obtained.

Abstract: According to the present invention, immortalized cells derived from porcine kidney macrophages are found to have high infection susceptibility to various African swlne fever virus strains. As a result, using the cells, it is possible to proliferate the virus and furthermore produce and detect the virus.

Abstract: An object of the present invention is to develop: a method of efficiently producing a long bagworm silk thread containing no contaminant while preventing a change in the spinning direction and the runaway of the bagworm from a rail, and alleviating a burden on the bagworm; and an apparatus for implementing the thread-producing method. Provided is an apparatus for producing a bagworm silk thread, having a movable rail having a width smaller than the maximum width between the right and left legs of a bagworm and configured to move in the longitudinal direction and to be able to hold with the legs of the bagworm; and a fixator configured to fix a bagworm, wherein the fixator is placed at a position such that the fixed bagworm can hold the movable rail.

Abstract: A simple and efficient breeding method for the bagworm moth is provided, as well as a device for performing the method. Also developed and provided is a method of easily collecting eggs from a bagworm moth, an egg collection device for bagworm moths composed of a tubular container. A bagworm moth breeding method and egg collection method performed using the device are also described.

Abstract: An object of the present invention is to develop a method of producing pure and long bagworm silk threads having no contaminants, such as pieces of leaves and twigs, and a device for implementing the production method. Provided is a method of producing a long bagworm silk thread, the method having a spinning process of making a bagworm having its nest hang its legs on a rail and continuously spin a silk thread along the rail, wherein the rail has a width smaller than the maximum width between the left and right legs when the bagworm used spreads out its legs and wherein the rail is such that the bagworm can hang its legs on the rail, and a collection process of collecting a long silk thread from the rail after the spinning process.

Abstract: A method for producing an HSL protein with increased catalytic activity to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner is disclosed. A method for producing a plant with increased resistance to a 4-HPPD inhibitor using the method for producing the HSL protein is also disclosed. It has been revealed that, by mutating position 140 to a basic amino acid in an HSL protein, the catalytic activity of the protein to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner can be increased, and an activity of the protein to decompose the inhibitor can be increased.

Abstract: Provided is a method of producing a long bagworm silk thread, the method comprising a spinning process of making a bagworm having its nest hang its legs on a rail and continuously spin a silk thread along the rail, wherein the rail has a width smaller than the maximum width between the left and right legs when the bagworm used spreads out its legs and wherein the rail is such that the bagworm can hang its legs on the rail, and a collection process of collecting a long silk thread from the rail after the spinning process.

Abstract: A production method for a dried Vitrigel film includes, in the following order: step 1 of concentrically arranging and disposing member A having one or more recesses and member B having one or more through holes, such that the recesses overlap the through holes; step 2 of pouring a sol from the through holes of member B; step 3 of gelling the sol; step 4 of drying and vitrifying the hydrogel obtained in step 3 in a state in which it is formed in the member A and the member B; step 5 of hydrating the dried hydrogel obtained in step 4; step 6 of drying and re-vitrifying the Vitrigel obtained in step 5; and step 7 of cutting off a portion, which slightly covers the top surface of the member A, of the dried Vitrigel obtained in step 6.

Abstract: The present invention provides a tomato plant which is resistant to ToBRFV. A Tobamovirus resistant tomato plant of the present invention is with loss of function for a SlTOM1a gene, a SlTOM1c gene, and a SlTOM1d gene.

Abstract: A thread collecting method for easily and simply collecting a bagworm-derived pure silk thread from a bagworm with a small number of processes and a thread collecting device for implementing the thread collecting method are developed and provided. A device that collects a silk thread from a bagworm is provided, the device including: a container that accommodates the bagworm; and an in-and-out hole through which the bagworm is put in and taken out of the container. A width of a maximum short axis cross section of an inner space of the container is in a range from 1.2 times or more to less than 3.1 times relative to a maximum body width of the bagworm being accommodated.

Abstract: A problem of this invention it to provide a method for differentiate a primordial germ cell into a functional GV stage oocyte by in vitro culture. This invention relates to a method for differentiating a primordial germ cell into a functional GV stage oocyte by in vitro culture, comprising: (a) a step of producing a secondary follicle by culturing the primordial germ cell and supporting cells adjacent to the primordial germ cells under conditions that eliminate the effects of estrogen or a factor having a similar function to estrogen; (b) a step of partially dissociating cells between a granulosa cell layer and a thecal cell layer, wherein an oocyte, the granulosa cell layer, and the thecal cell layer constitute the produced secondary follicle; and (c) a step of differentiating the oocyte into a functional GV stage oocyte by culturing the oocyte, the granulosa cell layer, and the thecal cell layer that constitute the secondary follicle in a medium containing a high-molecular-weight compound.

Abstract: A movement control method and a spinning position control method for a bagworm is provided, in which a bagworm is led to a predetermined position and allowed to spin a foothold silk thread at the predetermined position. A behavior control method in which a light is radiated to a bagworm to lead the bagworm toward a direction from which the light is radiated, and a spinning position control method in which a light is radiated to a bagworm at a spinning position on a surface of a base material to allow the bagworm to spin at the position are also provided.

Abstract: In order to provide: a method for producing an HSL protein with increased catalytic activity to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner; and a method for producing a plant with increased resistance to a 4-HPPD inhibitor using the method for producing the HSL protein, it has been revealed that, by mutating position 140 to a basic amino acid in an HSL protein, the catalytic activity of the protein to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner can be increased, and an activity of the protein to decompose the inhibitor can be increased.

Abstract: An object of the present invention is to develop and provide a fiber-reinforced composite material having an “elongation” feature in addition to a high strength and a high elastic modulus. Further, an object of the present invention is to address problems of brittleness and peeling in the conventional fiber-reinforced composite materials. A fiber-reinforced composite material containing the bagworm silk thread as reinforcing fibers is provided.

Abstract: The purpose of the present invention is to: provide an agent that effectively suppresses inhibition of antigen-antibody reaction in an immunoassay using a sample containing a body fluid, in particular, a component derived from a biological mucosal membrane, such as saliva; and to suppress false positive and false negative results in the immunoassay. The present invention provides an agent for suppressing inhibition of immune reaction, characterized in that the agent comprises a compound of the following (1) or (2): (1) Sulfonic acid compound of the formula R1—SO3H or a salt thereof. (In the formula, R1 is selected from the group consisting of: a straight-chain C5-C30 alkyl group; a straight-chain C1-C30 alkyl group substituted with an aryl group having at least one straight-chain C5-C30 alkyl group; and an aryl group having at least one straight-chain C5-C30 alkyl group. These groups may include a substituent group); and (2) Quaternary ammonium ion of the formula N+—R2R3R4R5 or a salt thereof.

Abstract: An object of the present invention is to develop: a method of efficiently producing a long bagworm silk thread containing no contaminant while preventing a change in the spinning direction and the runaway of the bagworm from a rail, and alleviating a burden on the bagworm; and an apparatus for implementing the thread-producing method. Provided is an apparatus for producing a bagworm silk thread, having a movable rail having a width smaller than the maximum width between the right and left legs of a bagworm and configured to move in the longitudinal direction and to be able to hold with the legs of the bagworm; and a fixator configured to fix a bagworm, wherein the fixator is placed at a position such that the fixed bagworm can hold the movable rail.

Abstract: An object of the present invention is to develop: a method of efficiently producing a long bagworm silk thread without slack of reeling during collection while preventing a change in the spinning direction and the runaway of the bagworm from a rail, and alleviating a burden on the bagworm; and an apparatus for implementing the thread-producing method. Provided is an apparatus for producing a bagworm silk thread, including: a movable loop-shaped rail configured to move in the longitudinal direction and to be held by the legs of a bagworm; a fixator configured to fix the bagworm; and an adhesion controller configured to store an adhesion control solution for washing away or removing a gummy component from the bagworm silk thread spun on the movable loop-shaped rail, wherein the apparatus makes it possible to cumulate the bagworm silk thread spun on the movable loop-shaped rail.

Abstract: A grafting device includes: a transfer unit configured to transfer, in a predetermined direction, a grafted seedling that is in a state where a cut surface of a graft and a cut surface of a rootstock are closely attached to each other; a tape supply unit configured to arrange a tape on a transfer path of the grafted seedling in such a manner that the tape is allowed to be pulled out; a pull-out member configured to transfer through the transfer path in advance of the grafted seedling to pull out the tape from the tape supply unit, and sandwich a closely attached part of the grafted seedling between a first part of the tape and a second part facing the first part; and a welding unit configured to cover a periphery of the closely attached part of the grafted seedling with the tape and weld the tape.

Abstract: Glucuronosyltransferase 1 gene which catalyzes glucuronic acid transfer to the hydroxyl group at the 3-position in an oleanane-type triterpenoid is identified. Glucuronosyltransferase 1 gene having a desired activity, derived from a Fabaceae plant (soybean, Glycyrrhiza, and Lotus japonicus), and containing nucleotide sequences represented by SEQ ID Nos: 2, 4, and 6, respectively, is provided.