Patents Assigned to National University Corporation, Hamamatsu University School of Medicine
  • Patent number: 8463360
    Abstract: Presentation of images showing with high resolution the state of a surgical field during surgery is realized with a simple configuration. When performing surgery, a three-dimensional model is generate based on MRI images of a patient captured prior to surgery, and three-dimensional coordinates of each location on a surface of the patient are measured by scanning a laser beam over the surface and detecting laser beam reflected therefrom, and correspondence is made with the MRI image for each location of the surface (frameless/markerless: steps 100 to 116), the position of a surgical instrument is also detected by the laser beam, the position of the surgical instrument derived, and images of the surgical instrument combined onto the MRI images and displayed during surgery (steps 120 to 130).
    Type: Grant
    Filed: January 31, 2007
    Date of Patent: June 11, 2013
    Assignee: National University Corporation Hamamatsu University School of Medicine
    Inventors: Seiji Yamamoto, Susumu Terakawa, Toshihisa Takai, Katsuhiro Sato
  • Patent number: 8445202
    Abstract: It is intended to provide a non-invasive and convenient method of detecting a tumor marker for diagnosing colon cancer which is superior in sensitivity and specificity to the existing fecal occult blood test. More specifically speaking, a method of detecting a tumor marker for diagnosing colon cancer which comprises collecting biological sample which is immediately frozen using liquid nitrogen in some cases, homogenizing the sample in the presence of an inhibitor of an RNA digesting enzyme to give a suspension, extracting RNA from the obtained suspension, subjecting the extracted RNA to reverse transcription to give cDNA. amplifying the obtained cDNA and then detecting the thus amplified cDNA. This method is characterized by involving no procedure of separating cell components from the biological sample.
    Type: Grant
    Filed: September 3, 2010
    Date of Patent: May 21, 2013
    Assignees: Olympus Corporartion, National University Corporation, Hamamatsu University School of Medicine
    Inventor: Shigeru Kanaoka
  • Publication number: 20130090258
    Abstract: An object of the present invention is to provide a method for detecting a colorectal tumor, and particularly advanced adenoma and early cancer, by using a component contained in stool as an indicator. Provided is a method for detecting colorectal tumor using a marker gene, comprising: (A) a step for extracting RNA contained in stool collected from a subject, (B) a step for measuring the amount of RNA derived from a marker gene present in the RNA obtained in step (A), and (C) a step for comparing the amount of RNA derived from the marker gene measured in step (B) with preset threshold values for each type of marker gene; wherein, the marker gene is creatine kinase B (CKB) gene.
    Type: Application
    Filed: June 3, 2011
    Publication date: April 11, 2013
    Applicant: NATIONAL UNIVERSITY CORPORATION HAMAMATSU UNIVERSITY SCHOOL OF MEDICINES
    Inventors: Shigeru Kanaoka, Kenichi Yoshida, Yasushi Hamaya
  • Patent number: 8395116
    Abstract: A mass spectrometer capable of obtaining a clear microscopic observation image with high spatial resolution in real time, even during a mass analysis, without affecting the analysis is provided. An aperture 1a is formed in a stage 1 on which a sample plate 2 to be placed. The sample plate 2 is transparent or translucent. A microscopic observation unit, including an observation optical system 20 and a CCD camera 21, is provided below the stage 1 to observe the reverse side of the sample 3 through the aperture 1a of the stage 1 as well as the transparent sample plate 2. The observed image is displayed on the screen of a display unit 27. If the sample 3 is a slice of biological tissue, the sample image taken from the reverse side will be substantially the same as an image taken from the obverse side.
    Type: Grant
    Filed: April 26, 2011
    Date of Patent: March 12, 2013
    Assignees: Shimadzu Corporation, National University Corporation Hamamatsu University School of Medicine
    Inventors: Takahiro Harada, Kiyoshi Ogawa, Mitsutoshi Setou
  • Patent number: 8388539
    Abstract: Surgical operation supporting apparatus and method is disclosed in which based on a high-definition tomographic images of an operation site produced before surgery, a three-dimensional model of the operation site is generated, and a surface of the operation site is optically measured during the surgical operation, and further, first position information that represents a three-dimensional position of each of points on the surface is acquired. An unexposed portion of the operation site is measured with ultrasonic waves during the surgical operation, and second position information that represents a three-dimensional position of each of points in the unexposed portion is acquired. Based on the first position information and the second position information, displacement and distortion at a portion whose three-dimensional position is not known in the three-dimensional model is estimated to obtain an estimated result.
    Type: Grant
    Filed: March 30, 2011
    Date of Patent: March 5, 2013
    Assignees: National University Corporation Hamamatsu University School of Medicine, National University Corporation Shizuoka University
    Inventors: Seiji Yamamoto, Susumu Terakawa, Toshihisa Takai, Katsuhiro Sato, Keiichi Abe, Baigalmaa Tsagaan
  • Publication number: 20130052127
    Abstract: The invention provides an antigen or drug delivery complex containing a complex of an antigen or drug and a cationic molecule, and an anionic molecule encapsulating the same. The antigen or drug delivery complex can be used as a main component of a drug delivery system that delivers various antigens and drugs to a particular cell or organ.
    Type: Application
    Filed: February 24, 2011
    Publication date: February 28, 2013
    Applicants: NAGASAKI UNIVERSITY, National University Corporation Hamamatsu University School of Medicine, KYUSYU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION
    Inventors: Hitoshi Sasaki, Tomoaki Kurosaki, Takashi Kitahara, Hideto To, Katsuyuki Yui, Kenji Hirayama, Kouichi Morita, Takahiro Mukai, Yasuhiro Magata, Mikako Ogawa, Kohei Sano
  • Patent number: 8365921
    Abstract: To provide a cell sorting method and a cell sorter which are capable of performing sorting focusing on various changes in each cell as stress on living cells is kept as low as possible, and proliferating the sorted cells. The present invention performs cell sorting using a fiber unit in which a plurality of fibers is bundled so as to be separable and unitable. In the present invention, a cell group containing target cells targeted for sorting is dispersed in a fluid into which the fiber unit is immersed, target fibers to which the target cells are attached among the plurality of fibers, are separated from the fiber unit, and the target cells are proliferated by use of the separated target fibers.
    Type: Grant
    Filed: September 17, 2009
    Date of Patent: February 5, 2013
    Assignee: National University Corporation Hamamatsu University School of Medicine
    Inventors: Takashi Sakurai, Susumu Terakawa, Remi Susuki, Hideo Mogami
  • Publication number: 20120295264
    Abstract: An object of the present invention is to provide a method of detecting an inflammatory bowel disease, and particularly ulcerative colitis, by using a component contained in fecal matter as an indicator. Namely, the present invention provides a method for detecting ulcerative colitis comprising: (A) a step of extracting RNA contained in fecal matter collected from a subject, (B) a step of measuring the amount of RNA derived from a marker gene in the RNA obtained in step (A), and (C) a step of comparing the amount of the RNA derived from the marker gene measured in step (B) with a preset threshold value, wherein the marker gene is one or more types of genes selected from the group consisting of COX-2 gene, B2M gene, MMP-7 gene, Snail gene, CD45 gene and CEA gene.
    Type: Application
    Filed: August 6, 2012
    Publication date: November 22, 2012
    Applicants: OLYMPUS CORPORATION, NATIONAL UNIVERSITY CORPORATION HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE
    Inventors: Shigeru Kanaoka, Yasushi Hamaya, Kenichi Yoshida
  • Publication number: 20120278037
    Abstract: Provided is a technique for using an optical microscope image of an area on a sample to collect area-specific information characterizing each kind of biological tissue from imaging mass analysis data. On an optical image of a two-dimensional target area on a sample, a difference is examined in the kind of tissue or other features and areas are specified, each regarded as the same kind of tissue. When data processing is initiated, peak information is extracted, for each specified area, from mass spectrum data of all the measurement points. A peak method is applied to each area to extract peak information. Then, when a command to compare a set of areas is given, the peak information of those areas is collected. By comparing the peak information of different areas by a machine learning algorithm or similar judging technique, area-specific peak information is obtained, and this information is stored in memory.
    Type: Application
    Filed: April 27, 2012
    Publication date: November 1, 2012
    Applicants: JAPANESE FOUNDATION FOR CANCER RESEARCH, SHIMADZU CORPORATION, National University Corporation Hamamatsu University School Of Medicine, KEIO UNIVERSITY
    Inventors: Masaaki MATSUURA, Masaru USHIJIMA, Masatoshi WAKUI, Mitsutoshi SETOU, Shigeki KAJIHARA, Kiyoshi OGAWA
  • Patent number: 8251893
    Abstract: A device for displaying assistance information for surgical operation 1 includes an endoscope 11, a CT device 30, an image pickup device 20, a surface shape calculating unit 43, a coordinate axis matching unit 44 that matches the shape of the patient surface by the CT device 30 and the shape of the patient surface calculated from the image, an endoscope optical axis calculating unit 45 that calculates a ray indicative of an optical axis in the image pickup direction of the endoscope 11, an intersection calculating unit 46 that calculates an intersection of the ray and the plane constituting the internal parts of the patient 60, and an outputting unit 47 that outputs the information indicative of the intersection after overlapping the information on the information indicative of the shape of the patient 60.
    Type: Grant
    Filed: January 9, 2008
    Date of Patent: August 28, 2012
    Assignee: National University Corporation Hamamatsu University School of Medicine
    Inventors: Seiji Yamamoto, Susumu Terakawa, Masanori Takaya
  • Patent number: 8241238
    Abstract: A cell sorting apparatus includes an extracorporeal circulation system 10 for sampling blood from a subject and circulating the blood outside a body; a cell measuring section 20 for supplying measurement light to cells contained in the blood to optically measure cell information on the physical quantity relating to the volume of the cell itself or a nucleus of the cell; and a cell separating section 30 for separating cells with reference to the cell information measured. The cell separating section 30 separates cells, based on the cell information measured by the cell measuring section 20 and a sorting condition set for sorting a specific type of cell such as a cancer cell, the cells satisfying the sorting condition, and returns the other cells into the body via the extracorporeal circulation system 10. Thus, a cell sorting apparatus can be realized which is capable of suitably sorting a target cell from the cells in blood.
    Type: Grant
    Filed: May 15, 2007
    Date of Patent: August 14, 2012
    Assignees: Hamamatsu Photonics K.K., National University Corporation Hamamatsu University School of Medicine
    Inventors: Teruo Hiruma, Hidenao Iwai, Susumu Terakawa
  • Patent number: 8208982
    Abstract: An evanescent catheter system having a tip portion of an optical fiber capable of securely measuring fluorescence intensity or fluorescence spectrum of a substance around a catheter tip, without being hindered by optical absorption of hemoglobin in erythrocytes in blood, and being capable of calculating an existing amount of a fluorescence-emitting substance existing in an evanescent light generating part. A protecting layer 22 and a cladding 23 are removed from an upper semicircular part of a core 24 at a tip of a catheter 21 to expose the core 24. When excitation light 29 is incident, evanescent light 25 is generated on the upper semicircular part of the core 24 and a fluorescent substance generates emits fluorescence 30. An interference filter 26 filters out the excitation light and only the fluorescence 30 reaches the photodiode 28, permitting a measuring device to measure an existing amount of the fluorescent substance.
    Type: Grant
    Filed: December 1, 2005
    Date of Patent: June 26, 2012
    Assignee: National University Corporation Hamamatsu University School of Medicine
    Inventors: Atsuo Miyakawa, Matsuyuki Doi, Seiji Yamamoto, Susumu Terakawa
  • Publication number: 20120133671
    Abstract: In an imaging mass analysis, image information of a sample allows users to grasp specific information about the sample, such as distribution of a portion with a particular function or effect. The mass spectrum intensity data are normalized for each pixel so that the sum of the intensities over the entire mass-to-charge ratio (m/z) is one. Entropy is calculated by totaling the product of the intensity normalizing at each m/z and the logarithm of that intensity over the entire m/z range. After the entropy is calculated for each pixel, the pixels are colored according to their entropy values to display a two-dimensional color image of entropy distribution. The entropy of a cancerous portion is relatively small because of a high content of a specific kind of substance and the simplified composition of the substances. Thus, the cancerous part and the normal part of the entropy image can be distinguished.
    Type: Application
    Filed: November 28, 2011
    Publication date: May 31, 2012
    Applicants: SHIMADZU CORPORATION, NATIONAL UNIVERSITY CORPORATION HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE
    Inventors: Mitsutoshi SETOU, Shigeki KAJIHARA
  • Publication number: 20120123254
    Abstract: The present invention has an object to measure lymphatic pressure with more safety and ease at lower cost. To achieve this, a lymphatic pressure-measuring system 1 includes: a manchette 11 fitted on a vital observation portion; a measurement unit 13 that measures and outputs pressure of the manchette 11; an infrared camera 21 that detects fluorescence emitted from fluorescent dye previously injected into a lymph vessel in the vital observation portion; and an image processing device 22 that generates and displays an image showing a position of the fluorescent dye in the lymph vessel based on a detection result of the infrared camera 21. The infrared camera 21 repeats the detection while the pressure of the manchette 11 decreases from first pressure to block a lymph flow in the vital observation portion to second pressure at restart of the lymph flow. The measurement unit 13 repeats the measurement during the period.
    Type: Application
    Filed: February 5, 2010
    Publication date: May 17, 2012
    Applicant: National University Corporation Hamamatsu University School of Medicine
    Inventor: Naoki Unno
  • Patent number: 8112144
    Abstract: Since cerebral atrophy does not occur only in a specific cross-section and reaches the brain in its entirety or arises prominently in a specific lobe (for example temporal lobe), for the assessment of cerebral atrophy, not only atrophy assessment for the frontal lobe but also an assessment also including atrophy of the temporal lobe, the parietal lobe and the occipital lobe is more desirable. The intracranial volume, the volume of the grey matter and the volume of the white matter are respectively extracted, computed and converted into numbers by image processing from a plurality of MRI slice images and the like. Ratios of these values from the conversions into numbers are taken to calculate the ratio of the grey matter and the ratio of the white matter with respect to the entire brain. Through comparison of a multitude of measurement data obtained by this automated computation, and a case, an objective cerebral atrophy assessment is carried out.
    Type: Grant
    Filed: March 29, 2007
    Date of Patent: February 7, 2012
    Assignees: National University Corporation Shizuoka University, National University Corporation Hamamatsu University School of Medicine
    Inventors: Seiji Yamamoto, Baigalmaa Tsagaan, Keiichi Abe
  • Publication number: 20120028879
    Abstract: Provided is a method that involves the detection of protein antigen (PA) and/or collagen-binding protein (CBP) of oral cavity bacteria in a sample, and in which oral cavity bacteria that exacerbate hemolysis are detected for and/or subjects at high-risk for hemolysis aggravation are screened anchor the level of risk of the hemolysis aggravation in a subject is assessed if a PA is not detected anchor a CBP is detected in the sample. Also provided are a detection reagent and kit for use in the method.
    Type: Application
    Filed: March 15, 2010
    Publication date: February 2, 2012
    Applicant: NATIONAL UNIVERSITY CORPORATION HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE
    Inventors: Kazuo Umemura, Kazuya Hokamura, Kazuhiko Nakano, Takashi Ooshima, Ryota Nomura, Koichiro Wada
  • Publication number: 20120010498
    Abstract: Surgical operation supporting apparatus and method is disclosed in which based on a high-definition tomographic images of an operation site produced before surgery, a three-dimensional model of the operation site is generated, and a surface of the operation site is optically measured during the surgical operation, and further, first position information that represents a three-dimensional position of each of points on the surface is acquired. An unexposed portion of the operation site is measured with ultrasonic waves during the surgical operation, and second position information that represents a three-dimensional position of each of points in the unexposed portion is acquired. Based on the first position information and the second position information, displacement and distortion at a portion whose three-dimensional position is not known in the three-dimensional model is estimated to obtain an estimated result.
    Type: Application
    Filed: March 30, 2011
    Publication date: January 12, 2012
    Applicant: National University Corporation Hamamatsu University School of Medicine
    Inventors: Seiji Yamamoto, Susummu Terakawa, Toshihisa Takai, Katsuhiro Sato, Keiichi Abe, Baigalmaa Tsagaan
  • Publication number: 20110266438
    Abstract: A mass spectrometer capable of obtaining a clear microscopic observation image with high spatial resolution in real time, even during a mass analysis, without affecting the analysis is provided. An aperture 1a is formed in a stage 1 on which a sample plate 2 to be placed. The sample plate 2 is transparent or translucent. A microscopic observation unit, including an observation optical system 20 and a CCD camera 21, is provided below the stage 1 to observe the reverse side of the sample 3 through the aperture 1a of the stage 1 as well as the transparent sample plate 2. The observed image is displayed on the screen of a display unit 27. If the sample 3 is a slice of biological tissue, the sample image taken from the reverse side will be substantially the same as an image taken from the obverse side.
    Type: Application
    Filed: April 26, 2011
    Publication date: November 3, 2011
    Applicants: National University Corporation Hamamatsu University School of Medicine, SHIMADZU CORPORATION
    Inventors: Takahiro HARADA, Kiyoshi OGAWA, Mitsutoshi SETOU
  • Publication number: 20110054300
    Abstract: Presentation of images showing with high resolution the state of a surgical field during surgery is realized with a simple configuration. When performing surgery, a three-dimensional model is generate based on MRI images of a patient captured prior to surgery, and three-dimensional coordinates of each location on a surface of the patient are measured by scanning a laser beam over the surface and detecting laser beam reflected therefrom, and correspondence is made with the MRI image for each location of the surface (frameless/markerless: steps 100 to 116), the position of a surgical instrument is also detected by the laser beam, the position of the surgical instrument derived, and images of the surgical instrument combined onto the MRI images and displayed during surgery (steps 120 to 130).
    Type: Application
    Filed: January 31, 2007
    Publication date: March 3, 2011
    Applicant: National University Corporation Hamamatsu University School of Medicine
    Inventors: Seiji Yamamoto, Susumu Terakawa, Toshihisa Takai, Katsuhiro Sato
  • Patent number: 7706060
    Abstract: The invention relates to a microscopic cell observation and inspection system that uses a total internal reflection cell illuminator that is capable of freely changing an observation position without recourse to any special slide glass, makes sure high SN-ratio observation and facilitates sample manipulation, thereby making high-sensitivity, fast detection of a lot of cell reactions on the same slide glass. While, in response to a command from personal computer (80), step motors (53, 54) are driven to sequentially scan observation positions of cell sample (S) on slide glass (21), one of shutter units (71) and (72) is closed and the other is opened at high speed, whereby either one of illumination optical paths for a TIRF microscope and a drop fluorescence microscope is selected to illuminate cell sample (S) on that observation position.
    Type: Grant
    Filed: September 13, 2006
    Date of Patent: April 27, 2010
    Assignees: National University Corporation Hamamatsu University School of Medicine, Keio University
    Inventors: Hideo Mogami, Yohei Sato, Yutaka Kazoe