Patents Assigned to Phyton Holdings, LLC
  • Patent number: 10822628
    Abstract: The present invention relates to a method of producing sesquiterpene lactones of the thapsigargin family, the method comprising the steps of: (a) culturing plant cells of the genus Thapsia in a nutrient medium in a suspension cell culture, wherein the cells produce one or more sesquiterpene lactones of the thapsigargin family; and (b) recovering one or more sesquiterpene lactones of the thapsigargin family produced in (a). The present invention further relates to a suspension cell culture comprising plant cells of the genus Thapsia, wherein the plant cells are capable of producing one or more sesquiterpene lactones of the thapsigargin family and to a plant cell biomass comprising plant cells of the genus Thapsia obtained from said suspension cell culture.
    Type: Grant
    Filed: December 2, 2014
    Date of Patent: November 3, 2020
    Assignee: Phyton Holdings, LLC
    Inventors: Gilbert Gorr, Harald Heckenmüller, David Alexander Ullisch, Jens Stefan Wilke, Yantree Devi Sankar-Thomas
  • Patent number: 10172306
    Abstract: The present invention relates to a method of producing Ingenol, Ingenol esters and/or Tiglian-3-one derivatives, the method comprising the steps of: (a) culturing plant cells obtained from a plant selected from the family Euphorbiaceae in a nutrient medium in a suspension cell culture, wherein the cells produce Ingenol, one or more Ingenol esters and/or one or more Tiglian-3-one derivatives; and (b) recovering the Ingenol, the one or more Ingenol esters and/or the one or more Tiglian-3-one derivatives produced in (a). The present invention further relates to a plant suspension cell culture, wherein the cells are obtained from a plant selected from the family Euphorbiaceae, and wherein the plant cells produce Ingenol and/or one or more Ingenol ester and/or one or more Tiglian-3-one derivatives.
    Type: Grant
    Filed: March 18, 2016
    Date of Patent: January 8, 2019
    Assignee: Phyton Holdings, LLC
    Inventors: Gilbert Gorr, David Alexander Ullisch, Yantree Devi Sankar-Thomas, Thomas Selge, Thomas Leibold, Harald Heckenmüller
  • Patent number: 9926287
    Abstract: A method of crystallizing docetaxel is disclosed. The method comprises (a) combining docetaxel and at least one solubilizing solvent to obtain a solution, wherein the solubilizing solvent is selected from the group consisting of acetone, methyl-tert-butyl ether, tetrahydrofuran (THF), and acetonitrile and (b) adding an anti-solvent to the solution to obtain a crystalline docetaxel, wherein the anti-solvent is water.
    Type: Grant
    Filed: November 18, 2016
    Date of Patent: March 27, 2018
    Assignee: Phyton Holdings, LLC
    Inventors: Brian A. Bucher, Edward M. Desimone, III, Roland R. Franke, Rex T. Gallagher, Barry J. Hand, Christopher D. Howe, James H. Johnson, John S. Juchum, Marc A. Plante, T.G. Sambandam, Dong S. Yang
  • Publication number: 20180042194
    Abstract: The present invention relates to a method of producing Ingenol, Ingenol esters and/or Tiglian-3-one derivatives, the method comprising the steps of: (a) culturing plant cells obtained from a plant selected from the family Euphorbiaceae in a nutrient medium in a suspension cell culture, wherein the cells produce Ingenol, one or more Ingenol esters and/or one or more Tiglian-3-one derivatives; and (b) recovering the Ingenol, the one or more Ingenol esters and/or the one or more Tiglian-3-one derivatives produced in (a). The present invention further relates to a plant suspension cell culture, wherein the cells are obtained from a plant selected from the family Euphorbiaceae, and wherein the plant cells produce Ingenol and/or one or more Ingenol ester and/or one or more Tiglian-3-one derivatives.
    Type: Application
    Filed: March 18, 2016
    Publication date: February 15, 2018
    Applicant: Phyton Holdings, LLC
    Inventors: Gilbert Gorr, David Alexander Ullisch, Yantree Devi Sankar-Thomas, Thomas Selge, Thomas Leibold, Harald Heckenmüller
  • Publication number: 20170275599
    Abstract: A plant cell having an animal-type sugar chain adding function is provided. The plant cell has an introduced gene encoding an enzyme derived from an animal, and the enzyme can transfer a fucose residue to a reducing terminal acetylglucosamine residue of a sugar chain of a glycoprotein.
    Type: Application
    Filed: November 21, 2016
    Publication date: September 28, 2017
    Applicant: Phyton Holdings, LLC
    Inventors: Kazuhito Fujiyama, Tatsuji Seki, Naoyuki Taniguchi
  • Patent number: 9574218
    Abstract: A method for the secretory production of a glycoprotein having a human-type sugar chain, comprising a step of introducing a gene of an enzyme capable of performing a transfer reaction of a galactose residue to a non-reducing terminal acetylglucosamine residue, and a gene of heterologous glycoprotein, to obtain a transformed plant cell, a step of culturing the plant cell, and a step of recovering the culture medium of the plant cell. The method further includes isolating the glycoprotein from the culture medium and contacting the isolated glycoprotein with a sialic acid transferase to add a sialic acid residue attached to the galactose residue in the N-glycan.
    Type: Grant
    Filed: April 14, 2014
    Date of Patent: February 21, 2017
    Assignee: Phyton Holdings, LLC
    Inventors: Kazuhito Fujiyama, Tatsuji Seki, Toshiomi Yoshida
  • Patent number: 9527825
    Abstract: Disclosed is a method of crystallizing docetaxel comprising combining docetaxel and at least one solubilizing solvent to obtain a solution, wherein the solubilizing solvent is methylene chloride and adding an anti-solvent to the solution to obtain a crystalline docetaxel, wherein the anti-solvent is water.
    Type: Grant
    Filed: February 25, 2016
    Date of Patent: December 27, 2016
    Assignee: Phyton Holdings, LLC
    Inventors: Brian A. Bucher, Edward M. Desimone, III, Roland R. Franke, Rex T. Gallagher, Barry J. Hand, Christopher D. Howe, James H. Johnson, John S. Juchum, Marc A. Plante, T. G. Sambandam, Dong S. Yang
  • Publication number: 20160312255
    Abstract: The present invention relates to a method of producing sesquiterpene lactones of the thapsigargin family, the method comprising the steps of: (a) culturing plant cells of the genus Thapsia in a nutrient medium in a suspension cell culture, wherein the cells produce one or more sesquiterpene lactones of the thapsigargin family; and (b) recovering one or more sesquiterpene lactones of the thapsigargin family produced in (a). The present invention further relates to a suspension cell culture comprising plant cells of the genus Thapsia, wherein the plant cells are capable of producing one or more sesquiterpene lactones of the thapsigargin family and to a plant cell biomass comprising plant cells of the genus Thapsia obtained from said suspension cell culture.
    Type: Application
    Filed: December 2, 2014
    Publication date: October 27, 2016
    Applicant: Phyton Holdings, LLC
    Inventors: Gilbert Gorr, Harald Heckenmuller, David Alexander Ullisch, Jens Stefan Wilke, Yantree Devi Sankar-Thomas
  • Patent number: 9303004
    Abstract: Disclosed is a method of crystallizing a taxane comprising combining the taxane and methylene chloride to obtain a solution and adding an anti-solvent to the solution to obtain a crystalline taxane, wherein the anti-solvent is selected from the group consisting of hexane and heptane.
    Type: Grant
    Filed: February 24, 2014
    Date of Patent: April 5, 2016
    Assignee: Phyton Holdings, LLC
    Inventors: James H. Johnson, T. G. Sambandam, Barry J. Hand, Christopher D. Howe, Roland R. Franke, Brian A. Bucher, John S. Juchum, Rex T. Gallagher, Marc A. Plante, Edward M. Desimone, III, Dong S. Yang
  • Publication number: 20150065714
    Abstract: The invention relates to the production of alkaloids from Liliaceae cell culture.
    Type: Application
    Filed: August 29, 2014
    Publication date: March 5, 2015
    Applicant: Phyton Holdings, LLC
    Inventors: Venkatesh SRINIVASAN, Jennifer Alford, Beth Slusar-Place, Charles Swindell, Michael Horn, Khisal Alvi, Gilbert Gorr
  • Publication number: 20150005517
    Abstract: Disclosed is a method of crystallizing a taxane comprising combining the taxane and methylene chloride to obtain a solution and adding an anti-solvent to the solution to obtain a crystalline taxane, wherein the anti-solvent is selected from the group consisting of hexane and heptane.
    Type: Application
    Filed: February 24, 2014
    Publication date: January 1, 2015
    Applicant: Phyton Holdings LLC
    Inventors: James H. Johnson, T.G. Sambandam, Barry J. Hand, Christopher D. Howe, Roland R. Franke, Brian A. Bucher, John S. Juchum, Rex T. Gallagher, Marc A. Plante, Edward M. Desimone, III, Dong S. Yang
  • Publication number: 20140377799
    Abstract: A method for the secretory production of a glycoprotein having a human-type sugar chain, comprising a step of introducing a gene of an enzyme capable of performing a transfer reaction of a galactose residue to a non-reducing terminal acetylglucosamine residue, and a gene of heterologous glycoprotein, to obtain a transformed plant cell, a step of culturing the plant cell, and a step of recovering the culture medium of the plant cell.
    Type: Application
    Filed: April 14, 2014
    Publication date: December 25, 2014
    Applicant: Phyton Holdings, LLC
    Inventors: Kazuhito Fujiyama, Tatsuji Seki, Toshiomi Yoshida
  • Patent number: 8877501
    Abstract: The present invention relates to the production of alkaloids from Liliaceae cell culture.
    Type: Grant
    Filed: June 18, 2010
    Date of Patent: November 4, 2014
    Assignee: Phyton Holdings, LLC
    Inventors: Venkatesh Srinivasan, Jennifer Alford, Beth Slusar-Place, Charles Swindell, Michael Horn, Khisal Alvi, Gilbert Gorr
  • Patent number: 8853370
    Abstract: The present invention provides a method for manufacturing a glycoprotein having a human-type sugar chain comprising a step in which transformed plant cell is obtained by introducing to a plant cell the gene of glycosyltransfetase and the gene of an exogenous glycoprotein, and a step in which the obtained transformed plant cell is cultivated.
    Type: Grant
    Filed: July 11, 2012
    Date of Patent: October 7, 2014
    Assignee: Phyton Holdings, LLC
    Inventors: Tatsuji Seki, Kazuhito Fujiyama
  • Patent number: 8735656
    Abstract: A method for the secretory production of a glycoprotein having a human-type sugar chain, comprising a step of introducing a gene of an enzyme capable of performing a transfer reaction of a galactose residue to a non-reducing terminal acetylglucosamine residue, and a gene of heterologous glycoprotein, to obtain a transformed plant cell, a step of culturing the plant cell, and a step of recovering the culture medium of the plant cell.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: May 27, 2014
    Assignee: Phyton Holdings, LLC
    Inventors: Kazuhito Fujiyama, Tatsuji Seki
  • Patent number: 8703982
    Abstract: Disclosed is a method of crystallizing a taxane comprising combining the taxane and methylene chloride to obtain a solution and adding an anti-solvent to the solution to obtain a crystalline taxane, wherein the anti-solvent is selected from the group consisting of hexane and heptane.
    Type: Grant
    Filed: February 12, 2010
    Date of Patent: April 22, 2014
    Assignee: Phyton Holdings LLC
    Inventors: Brian A. Bucher, Edward M. Desimone, III, Roland R. Franke, Rex T. Gallagher, Barry J. Hand, Christopher D. Howe, James A. Johnson, John S. Juchum, Marc A. Plante, T. G. Sambandam, Dong S. Yang
  • Publication number: 20130164782
    Abstract: A method for the secretory production of a glycoprotein having a human-type sugar chain, comprising a step of introducing a gene of an enzyme capable of performing a transfer reaction of a galactose residue to a non-reducing terminal acetylglucosamine residue, and a gene of heterologous glycoprotein, to obtain a transformed plant cell, a step of culturing the plant cell, and a step of recovering the culture medium of the plant cell.
    Type: Application
    Filed: September 14, 2012
    Publication date: June 27, 2013
    Applicant: Phyton Holdings, LLC
    Inventors: Kazuhito FUJIYAMA, Tatsuji Seki
  • Publication number: 20130040391
    Abstract: The present invention provides a method for manufacturing a glycoprotein having a human-type sugar chain comprising a step in which transformed plant cell is obtained by introducing to a plant cell the gene of glycosyltransfetase and the gene of an exogenous glycoprotein, and a step in which the obtained transformed plant cell is cultivated.
    Type: Application
    Filed: July 11, 2012
    Publication date: February 14, 2013
    Applicant: Phyton Holdings, LLC.
    Inventors: Tatsuji SEKI, Kazuhito Fujiyama
  • Publication number: 20130017582
    Abstract: This invention provides methods whereby taxol, baccatin III, and other taxol-like compounds, or taxanes, can be produced in very high yield from all known Taxus species, e.g., brevifolia, canadensis, cuspidata, baccata, globosa, floridana, wallichiana, media and chinensis. Particular modifications of culture conditions (i.e., media composition and operating modes) have been discovered to enhance the yield of various taxanes from cell culture of all species of Taxus. Particularly preferred enhancement agents include silver ion or complex, jasmonic acid (especially the methyl ester), auxin-related growth regulators, and inhibitors of the phenylpropanoid pathway, such as 3,4-methylenedioxy-6-nitrocinnamic acid. These enhancement agents may be used alone or in combination with one another or other yield-enhancing conditions. While the yield of taxanes from plant cell culture of T.
    Type: Application
    Filed: September 14, 2012
    Publication date: January 17, 2013
    Applicant: Phyton Holdings, LLC
    Inventors: Venkataraman BRINGI, Prakash G. Kadkade, Christopher L. Prince, Braden L. Roach
  • Patent number: 8338143
    Abstract: This invention provides methods whereby taxol, baccatin III, and other taxol-like compounds, or taxanes, can be produced in very high yield from all known Taxus species, e.g., brevifolia, canadensis, cuspidata, baccata, globosa, floridana, wallichiana, media and chinensis. Particular modifications of culture conditions (i.e., media composition and operating modes) have been discovered to enhance the yield of various taxanes from cell culture of all species of Taxus. Particularly preferred enhancement agents include silver ion or complex, jasmonic acid (especially the methyl ester), auxin-related growth regulators, and inhibitors of the phenylpropanoid pathway, such as 3,4-methylenedioxy-6-nitrocinnamic acid. These enhancement agents may be used alone or in combination with one another or other yield-enhancing conditions. While the yield of taxanes from plant cell culture of T.
    Type: Grant
    Filed: December 17, 2010
    Date of Patent: December 25, 2012
    Assignee: Phyton Holdings, LLC
    Inventors: Venkataraman Bringi, Prakash Kadkade, Christopher Prince, Braden Roach