Abstract: This invention relates to transgenic Brassica plants, plant material and seeds, characterized by harboring a specific transformation event, particularly by the presence of a male-sterility gene, at a specific location in the Brassica genome. The invention also relates to the seed deposited a the ATCC which will grow into a male sterile, herbicide resistant plant. The invention further provides a process producing a transgenic cell of a Brassica plant and a method of identifying a transgenic plant, or cells or tissues thereof. A kit for identifying the transgenic plants comprising the elite event of the prsent invention is also described. The Brassica plants of the invention combine the male-sterility phenotype with optimal agronomic performance, genetic stability and adaptability to different genetic backgrounds.

Abstract: This invention relates to transgenic winter oilseed rape (WOSR) plants, plant material and seeds, characterized by harboring a specific transformation event. It pertains to winter oilseed rape plants, more particularly to a pair of winter oilseed rape plants, which is particularly suited for the production of hybrid seed. More specifically, one plant is characterized by being male-sterile, due to the presence in its genome of a male sterility gene. The invention further provides a method for producing hybrid seed, a process for producing a transgenic WOSR plant oil or plant, and a method to identify a transgenic plant, cell or tissue. A kit for identifying the transgenic plants comparing the elite event of the present invention is also described. The WOSR plants of the invention combine the ability to form hybrid seeds with optimal agronomic performance, generic stability and adaptability to different generic backgrounds.

Abstract: A plant is provided which contains at least one dehiscence zone (DZ)-selective chimeric gene incorporated in the nuclear genome of its cells, wherein said DZ-selective chimeric gene comprises the following operably linked DNA fragments:

Abstract: This invention pertains to rice plants, plant material and seeds characterized by harboring a specific transformation event particularly by the presence of the bar gene under control of a CaMV 35S promoter, at a specific location in the rice genome. The rice plants of the invention combine glufosinate tolerance with optimal overall agronomic performance, genetic stability and adaptability to different genetic backgrounds.

Abstract: A plant is provided which contains at least one dehiscence zone (DZ)-selective chimeric gene incorporated in the nuclear genome of its cells, wherein said DZ-selective chimeric gene comprises the following operably linked DNA fragments: a) a transcribed DNA region encoding: 1) a RNA which, when produced in cells of a particular DZ of the plant, prevents, inhibits or reduces the expression in DZ cells of an endogenous gene of the plant encoding a cell wall hydrolase, particularly an endopolygalacturonase, or, 2) a protein or polypeptide, which when produced in DZ cells, kills or disables them or interferes with their normal metabolism, physiology or development; b) a plant expressible promoter which directs expression of transcribed DNA region at least in DZ cells, provided that if transcribed DNA region encodes a protein or polypeptide, or encodes an antisense RNA or ribozyme directed to a sense RNA encoded by an endogenous gene that is expressed in plant in cells other than DZ cells, plant expressible promot

Abstract: The invention relates to the use of a deacetylase coding sequence for obtaining conditional sterility in wheat. The invention relates to vectors comprising a deacetylase coding sequence under control of promoters which direct stamen-selective expression in wheat, which are particularly suited for the production of wheat plants which can be made male-sterile upon application of an acetylated toxin.

Abstract: Improved barstar DNAs, such as synthetic barstar DNAs, produce high amounts of barstar protein in eucaryotic cells, particularly in plant cells, and especially in stamen cells such as tapetum cells. Synthetic barstar DNAs have less than 40% A and T nucleotides. Other improved barstar DNAs encode improved barstar proteins which have an N-terminus which starts with Met-Xaa wherein Xaa is Alanine, Valine, Glycine, Aspartic acid or Glutamic acid. Plan cells and plants contain the improved barstar DNAs and/or express the improved barstar protein.

Abstract: The invention relates to an elite event glufosinate tolerant rice plant transformed with a bar gene, such as a 35S-bar gene, where the gene is inserted into the location defined by SEQ ID [No] NO: 9 within the rice genome, and methods of making and using the glufosinate tolerant rice plant.

Abstract: New pathogen-induced promoters are provided, particularly nematode-induced promoters, which are characterized by their selective induction of expression in the vicinity of the pathogen infection sites, such as the fixed feeding cells induced by infection of the plant by nematodes. Further provided are chimeric genes comprising these promoters as regulatory elements, as well as transgenic plants, comprising those chimeric genes, which are less susceptible to pathogen infections.

Abstract: A process for integrating a DNA fragment into the genome of a cell of a monocotyledonous plant, the process comprising the steps of:1) incubating, prior to contacting with the DNA fragment, a culture of untransformed monocotyledonous plant cells on a medium comprising a plant phenolic compound, for a period of time sufficient to stimulate cell division and enhance competence for integration of foreign DNA; and2) contacting the untransformed cells with the DNA fragment under conditions in which the DNA fragment is taken up by the untransformed cells and is stably integrated in the genome of the untransformed cells, to generate transformed cells.

Abstract: The invention concerns a process for producing transgenic plant cells, which comprises: contacting a culture of plant cells with an inhibitor of poly-(ADP-ribose) polymerase, prior to transformation, for a period of time sufficient to reduce the response of the cultured cells to stress and to reduce their metabolism. The untransformed cells are then contacted with foreign DNA comprising at least one gene of interest under conditions in which the foreign DNA is taken up by the untransformed cells and the gene of interest is stably integrated in the nuclear genome of the untransformed cells to produce the transgenic cells. The transgenic plant cells are recovered from the culture.

Abstract: Chimaeric DNA sequence which encodes: 1) a transit peptide of a cytoplasmic precursor of a chloroplast protein or polypeptide of a plant and 2) a protein or polypeptide that is heterologous to the transit peptide. The chimaeric DNA sequence can be used as a vector for transforming a plant cell so that a chimaeric precursor of the heterologous protein or polypeptide is produced in the cytoplasm of the cell and the chimaeric precursor then transports the heterologous protein or polypeptide in vivo into a chloroplast of the cell.

Abstract: A plant, the nuclear genome of which is transformed with a foreign DNA sequence encoding a product which neutralizes the activity of another product which disrupts the metabolism, functioning and/or development selectively of the plant's flower cells, particularly reproductive organ cells, or seed cells or embryo cells. The foreign DNA sequence also optionally encodes a marker.

Abstract: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as spodoptera spp. and Agotis ipsilon, against Pyralidae such as Ostrinia nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains nd that encodes its respective toxin, is resistant to Lipidoptera. Each strain, itself, or its crystal, crystal protein, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combating Lepidoptera.

Abstract: A plant having in the nuclear genome of its cells foreign DNA comprisinga male-sterility gene comprising:a male-sterility DNA encoding a sterility RNA, protein or polypeptide which, when produced or overproduced in a stamen cell of the plant, significantly disturbs the metabolism, functioning and/or development of the stamen cell, and,a sterility promoter directing expression of the male-sterility DNA selectively in specific stamen cells of said plant, the male-sterility DNA being in the same transcriptional unit as, and under the control of, the sterility promoter, anda coregulating gene comprising:a coregulating DNA encoding a coregulating RNA, protein or polypeptide which is capable, when produced in plant cells wherein said sterility RNA, protein or polypeptide is produced, of sufficiently preventing the activity of said sterility RNA, protein or polypeptide,whereby said coregulating DNA is in a transcriptional unit which is different from the transcriptional unit of said sterility DNA.

Abstract: The present invention provides a rapid and efficient method for transforming monocotyledonous plants. The invention particularly relates to the use of either intact tissue capable of forming compact embryogenic callus or compact embryogenic callus obtained from such tissue to obtain transgenic monocotyledonous plants. The present invention also provides novel transgenic plants obtained by the transformation method of the present invention.

Abstract: The invention relates to modified Bacillus thurigiensis genes with improved expression in plant cells, their preparation and uses. The invention relates more particularly to DNA fragments encoding all or part of a Bt insecticidal crystal protein, modified by translationally neutral modification(s) in cryptic promoter(s) and/or abortive intron(s). The invention also discloses method of preparing such modified DNAs, and methods of protecting plants from an insect pest.

Abstract: Plants made resistant to insects by transforming their nuclear genome with two or more DNA sequences, each encoding a different non-competitively binding B. thuringiensis protoxin or insecticidal part thereof, preferably the toxin thereof.

Abstract: Four novel Bacillus thurungienisis strains which are deposited at the BCCM-LMG under accesion nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinia nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystals proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.

Abstract: This invention is directed to a method to maintain male sterility in corn by the genetic linkage via genetic engineering of a homozygous male-fertility gene with a color linked restorer and plant comprising said genes. Color genes disclosed as useful in this invention include those involved in anthocyanin biosynthesis, which in corn are under the control of as many as 20 or more genes. In particular, the use of the anthocyanin color genes R, B and C1, and specific regulatory elements sequences are taught.