Patents Assigned to Qiagen Gaithersburg, Inc.
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Patent number: 9885092Abstract: Provided are nucleic acids capable of hybridizing to HPV 16 and/or HPV 18 nucleic acids, in particular, mRNA encoding E2 and E6-7 gene products. Such nucleic acids are useful in methods of isolating RNA from a biological sample, methods and means for determining the presence of particular RNA splice-form variants in a biological sample, methods and means for determining the relative ratio of RNA ratios in a biological sample, methods and means for predicting the progression of precancerous cervical lesions, and methods and means for detecting disruption of genes or gene expression.Type: GrantFiled: February 23, 2012Date of Patent: February 6, 2018Assignee: QIAGEN GAITHERSBURG INC.Inventors: Brian Lowe, Anna K. Fulbright, Irina Nazarenko
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Patent number: 9873921Abstract: An ultrasonic sample evaluation process in which a vial containing a liquid medium is placed on a receptacle and a ultrasonic source signal is propagated upwards in the vertical direction through the liquid medium. A number of reflected ultrasonic signals is received at the bottom of the sample vial and evaluated to identify a pattern characteristic of a reflection off the meniscus, and the presence of a pattern characteristic of a collection implement located in the liquid medium. An estimate of the amount of liquid medium contained in the sample vial is generated based on a time characteristic of the pattern characteristic of a reflection off the meniscus, and a signal indicating the presence of a collection implement in the liquid medium is generated upon identifying such a pattern.Type: GrantFiled: October 22, 2012Date of Patent: January 23, 2018Assignee: Qiagen Gaithersburg, Inc.Inventor: Bradley Scott Thomas
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Patent number: 9797000Abstract: Provided are methods of isolating RNA from a biological sample, methods and means for determining the presence of particular RNA splice-form variants in a biological sample, methods and means for determining the relative ratio of RNA ratios in a biological sample, and methods and means for predicting the progression of precancerous cervical lesions.Type: GrantFiled: April 30, 2010Date of Patent: October 24, 2017Assignee: QIAGEN GAITHERSBURG INC.Inventors: Brian Lowe, Anna K. Fulbright, Irina Nazarenko
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Patent number: 9777264Abstract: The present disclosure relates to immobilization of a cell using a carboxylated surface by contacting the carboxylated surface with a sample comprising the cell for a sufficient time to permit the cell to bind to the carboxylated surface. The immobilized cell may then be separated from the remainder of the sample and further manipulated to isolate, concentrate, and/or analyze the cell or a component thereof.Type: GrantFiled: November 2, 2012Date of Patent: October 3, 2017Assignee: QIAGEN GAITHERSBURG, INC.Inventors: Brian Lowe, Irina Nazarenko, Szymon Rus
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Patent number: 9689047Abstract: Methods and materials for determining the presence of at least one nucleic acid in a sample are provided, said methods comprising (1) a purification step using sequence specific hybrid capture; (2) an amplification step; and (3) a detection step using two separate sequence-specific polynucleotide probes. Also provided are nucleic acids comprising SEQ ID NO: 1 to SEQ ID NO: 727 and nucleic acid probes and probe sets comprising the same.Type: GrantFiled: January 28, 2011Date of Patent: June 27, 2017Assignee: QIAGEN GAITHERSBURG INC.Inventors: Dominic O'Neil, Irina Nazarenko, Holly Basham, Arvind Virmani, Shiuli Agarwal, Mridula Shukla
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Patent number: 9605303Abstract: Methods are provided for genotyping a target nucleic acid in a sample. In various aspects, the methods comprise generating nucleic acid hybrids between probes specific for the genotypes of interest and the target nucleic acid and detecting hybridization in the sample. In other aspects, the methods comprise using multi-probe mixtures to reduce the volume of sample necessary to determine the genotype of the target nucleic acid.Type: GrantFiled: January 28, 2011Date of Patent: March 28, 2017Assignee: QIAGEN GAITHERSBURG, INC.Inventor: Irina Nazarenko
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Patent number: 9593366Abstract: Disclosed are compositions and methods for random amplification of nucleic acid sequences of interest using random-G-deficient primers. Also disclosed are methods of randomly amplifying a target nucleic acid sequence using random G-deficient primers alone or in combination with random, partially random, or specific primers.Type: GrantFiled: October 26, 2009Date of Patent: March 14, 2017Assignee: QIAGEN GAITHERSBURG, INC.Inventors: Irina Nazarenko, Dominic O'Neil, Ha Thai, Attila Lorincz
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Patent number: 9435718Abstract: An automated sample tube decanting system having one or more decanting grippers, a decant waste receptacle, a transporter, and means for rotating the decanting gripper. Each decanting gripper has a respective holder configured to selectively mate with a respective interface on a tube strip. The one or more decanting grippers are rotatable between a first position in which the tube strip is upright and a second position in which the tube strip is inverted. The transporter is configured to move the one or more decanting grippers in a lateral direction from a starting location to a location above the decant waste receptacle.Type: GrantFiled: October 22, 2012Date of Patent: September 6, 2016Assignee: Qiagen Gaithersburg, Inc.Inventors: Bradley Scott Thomas, Brian Austin Self, Andrew Leonard, Bert Jungheim, Eric Schneider, Franklin Regan, Bowen Cui, Shubing Liu, Sharon Fluss
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Patent number: 9410146Abstract: The present invention provides compositions and methods for improving nucleic acid or protein recovery from fixed biological samples.Type: GrantFiled: September 14, 2010Date of Patent: August 9, 2016Assignee: QIAGEN GAITHERSBURG INC.Inventors: Shubing Liu, Suganthi Ramachandran
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Patent number: 9400234Abstract: An automated sample tube decanting system having one or more decanting grippers, a decant waste receptacle, a transporter, and means for rotating the decanting gripper. Each decanting gripper has a respective holder configured to selectively mate with a respective interface on a tube strip. The one or more decanting grippers are rotatable between a first position in which the tube strip is upright and a second position in which the tube strip is inverted. The transporter is configured to move the one or more decanting grippers in a lateral direction from a starting location to a location above the decant waste receptacle.Type: GrantFiled: October 22, 2012Date of Patent: July 26, 2016Assignee: Qiagen Gaithersburg, Inc.Inventors: Bradley Scott Thomas, Brian Austin Self, Andrew Leonard, Bert Jungheim, Eric Schneider, Franklin Regan, Bowen Cui, Shubing Liu, Sharon Fluss
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Patent number: 9376727Abstract: Strategically truncated probes specific for high-risk HPV nucleic acids, and methods for making and using the same, are disclosed herein. The disclosed probes, and methods of use thereof, permit fast and reliable detection of human papillomavirus in clinical samples without significant cross-reaction.Type: GrantFiled: May 24, 2011Date of Patent: June 28, 2016Assignee: QIAGEN GAITHERSBURG, INC.Inventors: Richard Mallonee, Peter Qiu
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Patent number: 9310281Abstract: An automated sample tube blotting system having one or more rotating grippers, a supply of absorbent material, a transporter, and an absorbent material removal mechanism. Each rotating gripper has a respective holder configured to selectively mate with a respective interface on a tube strip, and the one or more rotating grippers are rotatable between a first position in which the tube strip is upright and a second position in which the tube strip is inverted. The transporter is configured to move the rotating gripper in a lateral direction from a starting location to a location above the absorbent material, and in a vertical direction towards and away from the absorbent material.Type: GrantFiled: October 22, 2012Date of Patent: April 12, 2016Assignee: Qiagen Gaithersburg, Inc.Inventors: Bradley Scott Thomas, Brian Austin Self, Eric Schneider, Franklin Regan
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Patent number: 9260763Abstract: An automated process for converting samples includes: receiving tube strips having a number of sample tubes and samples therein, transferring multiple tube strips to a tube strip holder, dispensing sample conversion buffer into each tube, shaking the tube strip holder a first time, centrifuging the tube strip holder, removing a liquid supernatant from each tube, simultaneously inspecting the contents of each tube, dispensing a specimen transport medium and a denaturation reagent into each tube, shaking the tube strip holder a second time, heating the tube strip holder for a first length of time, shaking the tube strip holder a third time, heating the tube strip holder for a second length of time, shaking the tube strip holder a fourth time, and transferring at least a portion of each sample to a respective well on an output plate.Type: GrantFiled: October 22, 2012Date of Patent: February 16, 2016Assignee: Qiagen Gaithersburg, Inc.Inventors: Bradley Scott Thomas, Suganthi Ramachandran, Jason Troxell, Bowen Cui, Carl Theodore Edens, Andrew Leonard, Bert Jungheim
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Patent number: 9238809Abstract: The present disclosure relates to methods for isolating, amplifying, and/or analyzing nucleic acids in the presence of an anion exchange material by performing the isolation, amplification and/or analysis step in the presence of at least one anionic compound.Type: GrantFiled: August 5, 2010Date of Patent: January 19, 2016Assignees: QIAGEN GAITHERSBURG, INC., QIAGEN GMBHInventors: Yuri Khripin, Dirk Loeffert, Roland Fabis, Nadine Krueger
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Patent number: 9212976Abstract: A method for removing supernatant from a sample tube. The method includes providing a sample tube having a pellet at a bottom of the sample tube and a supernatant liquid above the pellet, visually inspecting the sample tube to determine one or more geometric properties of the pellet, and determining an expected height of a top surface of the pellet based on the one or more geometric properties determined in the visual inspection step. The method also includes inserting an aspirator into the supernatant liquid, moving the aspirator downwards towards the expected height of the top surface of the pellet, and aspirating the supernatant liquid through the aspirator.Type: GrantFiled: March 15, 2013Date of Patent: December 15, 2015Assignee: Qiagen Gaithersburg, Inc.Inventors: Bradley Scott Thomas, Edward Medri, Carl Theodore Edens, Brian Austin Self
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Patent number: 9180461Abstract: A cover for covering an array of sample tubes during an incubation process. The cover has an upper panel and protrusions extending downward from the upper panel. Each protrusion has an upper seal and a lower seal. The upper seal includes a first conical section that tapers from a first diameter that is larger than the inside diameter of a corresponding sample tube to a second diameter that is smaller than the inside diameter of the corresponding sample tube. The lower seal includes a cylindrical section extending downward from the bottom of the first conical section. The cylindrical section has a diameter selected to create a capillary seal, in the presence of a liquid, between the cylindrical section and an adjacent portion of an inner wall of the corresponding sample tube.Type: GrantFiled: October 22, 2012Date of Patent: November 10, 2015Assignee: Qiagen Gaithersburg, Inc.Inventors: Carl Theodore Edens, Andrew Leonard, Bert Jungheim, Peter Haviernik, Bowen Cui
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Patent number: 9135515Abstract: A method for visually inspecting pelletized samples. The method generates an inspection image of the bottom of a sample tube holder. The sample tube holder has a number of sample tube locations, and each sample tube location is configured to receive a sample tube in it. The inspection image is evaluated to determine whether a tube image exists for each sample tube location, and each sample tube image is evaluated to determine whether a pellet is located in each sample tube.Type: GrantFiled: October 22, 2012Date of Patent: September 15, 2015Assignee: Qiagen Gaithersburg, Inc.Inventors: Brian Bean, Steve Marlow, Bradley Scott Thomas
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Patent number: 9115410Abstract: Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA:RNA hybrids which can be detected by a variety of methods.Type: GrantFiled: March 15, 2013Date of Patent: August 25, 2015Assignee: QIAGEN GAITHERSBURG, INC.Inventors: Irina Nazarenko, Attila Lorincz, Paul Eder, Brian Lowe, Richard Mallonee, Ha Thai
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Patent number: 9107652Abstract: An automated sample aspirating method in which a magnetic force is applied to a collection tube containing a sample carrier to move at least the top of the carrier to a location off the center axis of the collection tube. An aspirator is inserted into the tube at a vertical position along the sample carrier, and a fluid is aspirated from the tube. Also provided are a sample collector having a tip adapted to obtain a sample from a sample source, and a handle that extends from the tip and is magnetic at a location remote from the tip. Also provided is a sample processing system having a sample collector such as described above, a collection tube adapted to hold the sample collector with the tip at a closed lower end of the tube and the handle extending towards an open upper end of the tube, and a cap that seals the sample collector tip and at least a portion of the handle inside the tube.Type: GrantFiled: November 19, 2009Date of Patent: August 18, 2015Assignee: Qiagen Gaithersburg, Inc.Inventors: Yancy Kenan Ragin, Bert Jungheim
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Patent number: D735881Type: GrantFiled: October 22, 2012Date of Patent: August 4, 2015Assignee: Qiagen Gaithersburg, Inc.Inventor: Bradley Scott Thomas