Abstract: According to an exemplary embodiment, a microneedle probe device for measuring a sap flow rate of a plant includes: a substrate, of which at least a part is inserted into a plant, and a thickness and a width are microscales; a single metal wire provided on the substrate; a power source, which applies a current to the metal wire for a predetermined time and heats the metal wire; and a processor, which calculates a flow rate of sap through a movement of heat generated in the metal wire according to a flow of the sap within the plant.

Abstract: The present invention relates to fusion polynucleotides for biosynthesis of beta-carotene and a method for producing beta-carotene using the same. More particularly, it relates to fusion polynucleotides encoding phytoene synthase, connective sequences of FMDV-derived 2A sequence or internal ribosome entry site (IRES) and carotene desaturase, and a method for producing beta-carotenes using the same. Fusion polynucleotides and the recombinant vector using the same of the present invention have the effect of expression both phytoene synthase gene and carotene desaturase gene stably within cell transformants. Accordingly, fusion polynucleotides of the present invention can be used to regulate the biosynthetic metabolism of plant producing beta-carotene. Furthermore, it can be applied to effectively increase the content of beta-carotene, a useful metabolite.

Abstract: A step-down sucking method is provided for minimizing the weaning stress of newborn calves. The method features feeding newborn calves with a sufficient amount of milk corresponding to 15-25% of body weight for 3-5 weeks following birth, and then with a stepwise decreased amount of milk corresponding finally to 8-15% of body weight for 3-7 days, with the differential amount replaced with water and then with gradually decreased amounts of milk from 8-15% to zero % of the body weight and with solid feedstuffs for a period from 5 weeks to 6-8 weeks after birth, thereby inducing the calves to maximally take solid feedstuff with minimal weaning stress.

Abstract: The present invention relates to fusion polynucleotides for biosynthesis of beta-carotene and a method for producing beta-carotene using the same. More particularly, it relates to fusion polynucleotides encoding phytoene synthase, connective sequences of FMDV-derived 2A sequence or internal ribosome entry site (IRES) and carotene desaturase, and a method for producing beta-carotenes using the same. Fusion polynucleotides and the recombinant vector using the same of the present invention have the effect of expression both phytoene synthase gene and carotene desaturase gene stably within cell transformants. Accordingly, fusion polynucleotides of the present invention can be used to regulate the biosynthetic metabolism of plant producing beta-carotene. Furthermore, it can be applied to effectively increase the content of beta-carotene, a useful metabolite.