Patents Assigned to Roche Diagnostics Hematology, Inc.
  • Patent number: 10775282
    Abstract: The systems and methods disclosed herein permit automated preparation of biological specimens for examination. The disclosed systems and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a biological specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation cycles for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining biological specimens.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: September 15, 2020
    Assignee: Roche Diagnostics Hematology, Inc
    Inventors: Daniel Lapen, David Zahniser, Mark Licari, Brian McKeen, Eric D. Yeaton, Dennis Poole
  • Patent number: 10764538
    Abstract: Systems and methods analyzing body fluids contain cells including blood, bone marrow, urine, vaginal tissue, epithelial tissue, tumors, semen, and spittle are disclosed. The systems and methods utilize an improved technique for applying a monolayer of cells to a slide and generating a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and method for utilizing multi-color microscopy for improving the quality of images captured by a light receiving device.
    Type: Grant
    Filed: March 20, 2017
    Date of Patent: September 1, 2020
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: James Winkelman, Milenko Tanasijevic, David Zahniser, James Linder
  • Patent number: 10656056
    Abstract: The formulations, systems, and methods disclosed herein permit automated preparation of specimens (e.g., biological specimens) for examination. The disclosed formulations, systems, and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation phases for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining specimens.
    Type: Grant
    Filed: May 10, 2017
    Date of Patent: May 19, 2020
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Daniel Lapen, Kerri Horton
  • Patent number: 10466146
    Abstract: Methods for dispensing a fluid sample on a substrate include obtaining an image of a sample applicator in proximity to the substrate, where the image includes a first image of the sample applicator and a second image of the sample applicator, determining a height of the sample applicator relative to a surface plane of the substrate based on a distance between common portions of the first and second images, and dispensing the fluid sample onto the substrate using the sample applicator, where the dispensing includes: translating the sample applicator, translating the substrate, or translating both the sample applicator and the substrate to effect a relative translation between the sample applicator and the substrate; and maintaining the sample applicator within 2 microns of a target height relative to the surface plane of the substrate during the translating.
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: November 5, 2019
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Russell Zahniser, David Zahniser, Stephen Conroy, Michael Zahniser
  • Patent number: 10462351
    Abstract: The disclosure relates to methods and systems for automatically focusing multiple images of one or more objects on a substrate. The methods include obtaining, by a processor, a representative focal distance for a first location on the substrate based on a set of focal distances at known locations on the substrate. The methods also include acquiring, by an image acquisition device, a set of at least two images of the first location. The images are each acquired using a different focal distance at an offset from the representative focal distance. The methods further include estimating, by a processor, an ideal focal distance corresponding to the first location based on comparing a quality of focus for each of the images, and storing the estimated ideal focal distance and the first location in the set of focal distances at known locations.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: October 29, 2019
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventor: Michael Zahniser
  • Patent number: 10345205
    Abstract: Sample application systems can include an extraction mechanism to remove a sample from sample containers, a sample vessel disposed on a deployment mechanism, where the deployment mechanism is arranged to move the sample vessel to receive a sample, an extraction mechanism washing station to wash the extraction mechanism, a sample applicator to remove a portion of the sample in the sample vessel and apply it onto a sample carrier, where the deployment mechanism can move the sample vessel to a sample application position, a sample vessel washing station to wash the sample vessel, where the deployment mechanism can move the sample vessel to the sample vessel washing station, a sample applicator washing station to wash the sample applicator after the sample has been dispensed onto the sample carrier, and a fluid control system to control flow of a fluid provided to the extraction mechanism and the sample applicator.
    Type: Grant
    Filed: March 6, 2017
    Date of Patent: July 9, 2019
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Brian J. McKeen, Eric D. Yeaton, James L. Dowling, David Zahniser, Stephen Conroy
  • Patent number: 10281382
    Abstract: A method for determining a mean cell volume for a blood sample includes: illuminating the sample with incident light at a plurality of illumination wavelengths and obtaining a two-dimensional image of the sample at each of the plurality of illumination wavelengths; identifying a plurality of cells that appear in each of the images; for each one of the plurality of cells, determining an integrated optical density corresponding to each of the plurality of illumination wavelengths; for each one of the plurality of cells, determining a cell volume based on the integrated optical densities corresponding to each of the plurality of illumination wavelengths; and determining the mean cell volume for the blood sample from the cell volumes for each one of the plurality of cells.
    Type: Grant
    Filed: March 6, 2017
    Date of Patent: May 7, 2019
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Michael Zahniser, Russell Zahniser
  • Patent number: 10175153
    Abstract: The systems and methods disclosed herein permit automated preparation of biological specimens for examination. The disclosed systems and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a biological specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation cycles for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining biological specimens.
    Type: Grant
    Filed: August 24, 2015
    Date of Patent: January 8, 2019
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Daniel Lapen, David Zahniser, Mark Licari, Brian J. McKeen, Eric D. Yeaton, Dennis Poole
  • Patent number: 10094764
    Abstract: Systems and methods analyzing body fluids such as blood and bone marrow are disclosed. The systems and methods may utilize an improved technique for applying a monolayer of cells to a slide to generate a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and methods for utilizing multi color microscopy for improving the quality of images captured by a light receiving device.
    Type: Grant
    Filed: April 27, 2015
    Date of Patent: October 9, 2018
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: James Winkelman, Milenko Tanasijevic, David Zahniser
  • Patent number: 10083342
    Abstract: This document describes methods, systems and computer program products directed to imaging blood cells. The subject matter described in this document can be embodied in a method of classifying white blood cells (WBCs) in a biological sample on a substrate. The method includes acquiring, by an image acquisition device, a plurality of images of a first location on the substrate, and classifying, by a processor, objects in the plurality of images into WBC classification groups. The method also includes identifying, by a processor, objects from at least some classification groups, as unclassified objects, and displaying, on a user interface, the unclassified objects and at least some of the classified objects.
    Type: Grant
    Filed: June 26, 2017
    Date of Patent: September 25, 2018
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Michael Zahniser, Kam Lin Wong, David Zahniser, Robert Hohlfeld
  • Patent number: 10068126
    Abstract: Systems, methods and computer program products for mapping coordinates of various imaging stations are described. In some implementations, cells (e.g., red blood cells) in a biological specimen can be used for determining the mapping information between the imaging stations. The use of cells allows a target image (e.g., an image of a sub-region of cells in the biological specimen) taken by one imaging station to be pattern-matched to a reference image (e.g., an image showing a larger region of cells in the biological specimen that also includes the sub-region) taken by another imaging station. Once the target image is matched to the reference image, point by point correspondence (and therefore coordinates) between the target image and the reference image can be established for computing the coordinate transformation to map the imaging stations.
    Type: Grant
    Filed: February 17, 2016
    Date of Patent: September 4, 2018
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Michael Zahniser, Russell Zahniser
  • Patent number: 9891147
    Abstract: The apparatus disclosed herein features a substrate arm and gripper, a platform having a top surface located opposite the substrate when the substrate arm is in a specimen processing position and featuring at least one fluid port located on a surface of the platform facing the substrate and at least one vacuum port located on the surface of the platform facing the substrate, and during operation, the apparatus is configured to (a) dispense a first quantity of fluid from the at least one fluid port to fill a separation between the substrate and the platform, (b) dispense an additional quantity of fluid from the at least one fluid port into the separation to displace a portion of the first quantity of fluid, and (c) remove the portion of the first quantity of fluid from the separation through the at least one vacuum port.
    Type: Grant
    Filed: April 4, 2014
    Date of Patent: February 13, 2018
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Daniel Lapen, David Zahniser, Mark Licari, Brian J. McKeen, Eric D. Yeaton, Dennis Poole, Stephen Conroy
  • Publication number: 20180012062
    Abstract: This document describes methods, systems and computer program products directed to imaging blood cells. The subject matter described in this document can be embodied in a method of classifying white blood cells (WBCs) in a biological sample on a substrate. The method includes acquiring, by an image acquisition device, a plurality of images of a first location on the substrate, and classifying, by a processor, objects in the plurality of images into WBC classification groups. The method also includes identifying, by a processor, objects from at least some classification groups, as unclassified objects, and displaying, on a user interface, the unclassified objects and at least some of the classified objects.
    Type: Application
    Filed: June 26, 2017
    Publication date: January 11, 2018
    Applicant: Roche Diagnostics Hematology, Inc.
    Inventors: Michael Zahniser, Kam Lin Wong, David Zahniser, Robert Hohlfeld
  • Patent number: 9813608
    Abstract: The disclosure relates to methods and systems for automatically focusing multiple images of one or more objects on a substrate. The methods include obtaining, by a processor, a representative focal distance for a first location on the substrate based on a set of focal distances at known locations on the substrate. The methods also include acquiring, by an image acquisition device, a set of at least two images of the first location. The images are each acquired using a different focal distance at an offset from the representative focal distance. The methods further include estimating, by a processor, an ideal focal distance corresponding to the first location based on comparing a quality of focus for each of the images, and storing the estimated ideal focal distance and the first location in the set of focal distances at known locations.
    Type: Grant
    Filed: May 22, 2015
    Date of Patent: November 7, 2017
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventor: Michael Zahniser
  • Patent number: 9784652
    Abstract: Methods for dispensing a fluid sample on a substrate include obtaining an image of a sample applicator in proximity to the substrate, where the image includes a first image of the sample applicator and a second image of the sample applicator, determining a height of the sample applicator relative to a surface plane of the substrate based on a distance between common portions of the first and second images, and dispensing the fluid sample onto the substrate using the sample applicator, where the dispensing includes: translating the sample applicator, translating the substrate, or translating both the sample applicator and the substrate to effect a relative translation between the sample applicator and the substrate; and maintaining the sample applicator within 2 microns of a target height relative to the surface plane of the substrate during the translating.
    Type: Grant
    Filed: February 22, 2016
    Date of Patent: October 10, 2017
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Russell Zahniser, David Zahniser, Stephen Conroy, Michael Zahniser
  • Patent number: 9690976
    Abstract: This document describes methods, systems and computer program products directed to imaging blood cells. The subject matter described in this document can be embodied in a method of classifying white blood cells (WBCs) in a biological sample on a substrate. The method includes acquiring, by an image acquisition device, a plurality of images of a first location on the substrate, and classifying, by a processor, objects in the plurality of images into WBC classification groups. The method also includes identifying, by a processor, objects from at least some classification groups, as unclassified objects, and displaying, on a user interface, the unclassified objects and at least some of the classified objects.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: June 27, 2017
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Michael Zahniser, Kam Lin Wong, David Zahniser, Robert Hohlfeld
  • Patent number: 9677977
    Abstract: The formulations, systems, and methods disclosed herein permit automated preparation of specimens (e.g., biological specimens) for examination. The disclosed formulations, systems, and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation phases for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining specimens.
    Type: Grant
    Filed: March 12, 2013
    Date of Patent: June 13, 2017
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Daniel Lapen, Kerri Horton
  • Patent number: 9602777
    Abstract: Systems and methods analyzing body fluids contain cells including blood, bone marrow, urine, vaginal tissue, epithelial tissue, tumors, semen, and spittle are disclosed. The systems and methods utilize an improved technique for applying a monolayer of cells to a slide and generating a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and method for utilizing multi-color microscopy for improving the quality of images captured by a light receiving device.
    Type: Grant
    Filed: April 27, 2010
    Date of Patent: March 21, 2017
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: James Winkelman, Milenko Tanasijevic, David Zahniser, James Linder
  • Patent number: 9588026
    Abstract: Sample application systems can include an extraction mechanism to remove a sample from sample containers, a sample vessel disposed on a deployment mechanism, where the deployment mechanism is arranged to move the sample vessel to receive a sample, an extraction mechanism washing station to wash the extraction mechanism, a sample applicator to remove a portion of the sample in the sample vessel and apply it onto a sample carrier, where the deployment mechanism can move the sample vessel to a sample application position, a sample vessel washing station to wash the sample vessel, where the deployment mechanism can move the sample vessel to the sample vessel washing station, a sample applicator washing station to wash the sample applicator after the sample has been dispensed onto the sample carrier, and a fluid control system to control flow of a fluid provided to the extraction mechanism and the sample applicator.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: March 7, 2017
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Brian J. McKeen, Eric D. Yeaton, James L. Dowling, David Zahniser, Stephen Conroy
  • Patent number: 9588033
    Abstract: A method for determining a mean cell volume for a blood sample includes: illuminating the sample with incident light at a plurality of illumination wavelengths and obtaining a two-dimensional image of the sample at each of the plurality of illumination wavelengths; identifying a plurality of cells that appear in each of the images; for each one of the plurality of cells, determining an integrated optical density corresponding to each of the plurality of illumination wavelengths; for each one of the plurality of cells, determining a cell volume based on the integrated optical densities corresponding to each of the plurality of illumination wavelengths; and determining the mean cell volume for the blood sample from the cell volumes for each one of the plurality of cells.
    Type: Grant
    Filed: December 10, 2014
    Date of Patent: March 7, 2017
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Michael Zahniser, Russell Zahniser