Abstract: Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.
Type:
Grant
Filed:
October 28, 2013
Date of Patent:
November 15, 2016
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Philip D. Gregory, Jeffrey C. Miller, David Paschon, Edward J. Rebar, Siyuan Tan, Fyodor Urnov, Lei Zhang
Abstract: Disclosed herein are enhanced polypeptides, polynucleotides encoding these polypeptides, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.
Type:
Grant
Filed:
November 16, 2012
Date of Patent:
October 4, 2016
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Philip D. Gregory, Jeffrey C Miller, David Paschon, Edward J. Rebar, Siyuan Tan, Fyodor Urnov, Lei Zhang
Abstract: Disclosed herein are methods and compositions for inactivating CCR-5 genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided.
Type:
Grant
Filed:
October 15, 2013
Date of Patent:
September 6, 2016
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Dale Ando, Michael C. Holmes, Gary Ka Leong Lee
Abstract: Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into a predetermined target site in a plant genome.
Type:
Grant
Filed:
August 9, 2007
Date of Patent:
August 30, 2016
Assignees:
Dow AgroSciences LLC, Sangamo BioSciences, Inc.
Inventors:
Qihua Cai, Jeffrey C. Miller, William M. Ainley, Robbi J. Garrison, Joseph F. Petolino, Beth C. Rubin-Wilson, Lisa L. Schulenberg, Andrew F. Worden
Abstract: Nucleases and methods of using these nucleases for expressing a transgene from a safe harbor locus in a secretory tissue, and clones and animals derived therefrom.
Abstract: Disclosed herein are compositions for linking DNA binding domains and cleavage domains (or cleavage half-domains) to form non-naturally occurring nucleases. Also described are methods of making and using compositions comprising these linkers.
Abstract: Disclosed herein are methods and compositions for inactivating a glutamine synthetase (GS) gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
Abstract: Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
Type:
Grant
Filed:
April 29, 2015
Date of Patent:
June 28, 2016
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Russell DeKelver, Philip D. Gregory, Michael C. Holmes, Fyodor Urnov
Abstract: Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.
Abstract: Disclosed herein are methods and compositions for inactivating a FUT8 gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
Abstract: Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.
Type:
Grant
Filed:
October 28, 2013
Date of Patent:
April 26, 2016
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Philip D. Gregory, Jeffrey C. Miller, David Paschon, Edward J. Rebar, Siyuan Tan, Fyodor Urnov, Lei Zhang
Abstract: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.
Type:
Grant
Filed:
March 15, 2013
Date of Patent:
March 22, 2016
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Fyodor Urnov, Michael C. Holmes, Jeffrey C. Miller, Carl O. Pabo
Abstract: Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into the human PPP1R12C locus, for example, for expression of a polypeptide of interest.
Type:
Grant
Filed:
July 30, 2014
Date of Patent:
February 23, 2016
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Russell DeKelver, Philip D. Gregory, David Paschon, Phillip Tam, Fyodor Urnov
Abstract: Disclosed herein are methods and compositions for correction and/or mutation of genes associated with Parkinson's Disease as well as clones and animals derived therefrom.
Type:
Grant
Filed:
December 15, 2011
Date of Patent:
February 23, 2016
Assignees:
Sangamo BioSciences, Inc., Whitehead Institute for Biomedical Research
Inventors:
Rudolf Jaenisch, Josee Laganiere, Frank Soldner, Lei Zhang
Abstract: Disclosed herein are methods and compositions for targeted integration of a exogenous sequence into a predetermined target site in a genome for use, for example, in protein expression and gene inactivation.
Type:
Grant
Filed:
March 6, 2013
Date of Patent:
February 16, 2016
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Philip D. Gregory, Michael C. Holmes, Edward J. Rebar, Fyodor Urnov
Abstract: Disclosed herein are donor molecules comprising single-stranded complementary regions flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.
Type:
Grant
Filed:
February 9, 2011
Date of Patent:
February 9, 2016
Assignee:
Sangamo BioSciences, Inc.
Inventors:
Gregory J. Cost, Dmitry M. Guschin, Fyodor Urnov
Abstract: Cells and methods of using these cells for expressing a transgene expressing a protein that is aberrantly expressed in a metabolic disorders from a safe harbor locus.
Type:
Grant
Filed:
December 4, 2013
Date of Patent:
February 9, 2016
Assignees:
Sangamo BioScience, Inc., The Children's Hospital of Philadelphia
Abstract: Disclosed herein are compositions and methods for generating chromosomal translocations and targeted deletions of specific lengths and at specific locations the genome of cell.